• Korean Journal of Environmental Agriculture
• /
• v.33 no.1
• /
• pp.1-8
• /
• 2014

BACKGROUND: Recent studies using many amendments for heavy metal stabilization in soil were conducted in order to find out new materials. But, the studies accounting for the use of appropriate amendments considering soil pH remain incomplete. The aim of this study was to investigate the effects of initial soil pH on the efficiency of various amendments. METHODS AND RESULTS: Acid soil and alkali soil contaminated with heavy metals were collected from the agricultural soils affected by the abandoned mine sites nearby. Three different types of amendments were selected with hypothesis being different in stabilization mechanisms; organic matter, lime stone and iron, and added with different combination. For determining the changes in the extractable heavy metals, water soluble, Mehlich-3, Toxicity Characteristic Leaching Procedure, Simple Bioavailability Extraction Test method were applied as chemical assessments for metal stabilization. For biological assessments, soil respiration and root elongation of bok choy (Brassica campestris ssp. Chinensis Jusl.) were determined. CONCLUSION: It was revealed that lime stone reduced heavy metal mobility in acid soil by increasing soil pH and iron was good at stabilizing heavy metals by supplying adsorption sites in alkali soil. Organic matter was a good source in terms of supplying nutrients, but it was concerning when accounting for increasing metal availability.

• Structural Engineering and Mechanics
• /
• v.37 no.2
• /
• pp.149-162
• /
• 2011

The aim of this research is a comprehensive review and evaluation of beam theories resting on elastic foundations that used to model mode-I delamination in multidirectional laminated composite by DCB specimen. A compliance based approach is used to calculate critical strain energy release rate (SERR). Two well-known beam theories, i.e. Euler-Bernoulli (EB) and Timoshenko beams (TB), on Winkler and Pasternak elastic foundations (WEF and PEF) are considered. In each case, a closed-form solution is presented for compliance versus crack length, effective material properties and geometrical dimensions. Effective flexural modulus ($E_{fx}$) and out-of-plane extensional stiffness ($E_z$) are used in all models instead of transversely isotropic assumption in composite laminates. Eventually, the analytical solutions are compared with experimental results available in the literature for unidirectional ($[0^{\circ}]_6$) and antisymmetric angle-ply ($[{\pm}30^{\circ}]_5$, and $[{\pm}45^{\circ}]_5$) lay-ups. TB on WEF is a simple model that predicts more accurate results for compliance and SERR in unidirectional laminates in comparison to other models. TB on PEF, in accordance with Williams (1989) assumptions, is too stiff for unidirectional DCB specimens, whereas in angle-ply DCB specimens it gives more reliable results. That it shows the effects of transverse shear deformation and root rotation on SERR value in composite DCB specimens.

• Journal of the Korean Dietetic Association
• /
• v.17 no.3
• /
• pp.229-242
• /
• 2011

The purpose of this study was to investigate the status of the safety system and safety education in institutional foodservices in the Changwon and Masan areas. The survey was conducted from February 1 to March 31, 2009 via questionnaires that were sent to 300 dietitians, and 142 dietitians responded. It was determined that most of the safety managers were 'dietitians (87.1%)', whereas facilities/equipment managers consisted of 'dietitians (45.7%)', 'department of facilities management in the organization (36.4%)' and 'outsourced company of facilities management (17.9%)'. Out of the 11 safety practices, seven safety practices showed less than 50% of total implementation ratio, which meant that the safety systems were not functioning properly. Except for 'non-skid shoes (85.9%)', other safety equipment was seldom used. The survey respondents recognized that safety education was very necessary (4.47 points); however, they responded so-so (3.46 points) to the question of whether or not the actual frequency and time spent on safety education were enough. The average time spent on safety education was 28 minutes 11 seconds. Regarding the difficulties in performing safety education, 'not many safety education materials and media (3.44 points)', 'not many varieties in the subjects and contents for safety education (3.40 points)', and 'not much organizational support on safety education (3.33 points)' showed higher scores than 'lack of education time due to workload (3.20 points)'. The following were cited as education materials that should be developed as it is currently difficult to obtain relevant information and data: 'root causes of musculoskeletal injury and preventive measures (15.8%)', 'healthcare and disease preventive exercises for employees who do simple and repetitive works (14.9%)', and 'instruction on safe handling of chemicals (12.7%)'

• Restorative Dentistry and Endodontics
• /
• v.30 no.3
• /
• pp.215-220
• /
• 2005

It is common for clinicians to encounter endodontically tl·treated teeth that contain posts within their roots. If endodontic treatment is failed, these posts must be removed to facilitate successful nonsurgical retreatment. There have been many techniques such as ultrasonic instrument, Ruddle post removal system, Eggler post remover and Masserann kit developed to facilitate removal of posts from the root canal space. But these methods may be disadvantageous because long length of time required for post removal and fracture of post or teeth. In now days new post removal technique using ATD automatic bridge remover was introduced. Advantages of this method are simple and short time consuming compare to others. This article served as a successful case report of post removal using ATD automatic bridge remover.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.2
• /
• pp.335-341
• /
• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

• Restorative Dentistry and Endodontics
• /
• v.25 no.3
• /
• pp.321-337
• /
• 2000

Dental caries, one of the most frequent dental disease, become larger because it can be thought as a simple disease. Further more, it can progress to unexpected root canal therapy with fabrication of crown that needs reduction of tooth structure. Base is required in a large caries and ZOE, ZPC, glass ionomer are used frequently as base material. They, with restorative material, can affect the longevity of the restoration. In this study, we assume that the mandibular 1st molar has deep class I cavity. So, installing the 3 base material, 3 kinds of fillings were restored over the base as follows; 1) amalgam only, 2) amalgam with ZPC, 3) amalgam with ZOE, 4) amalgam with GI cement, 5) gold inlay with ZPC, 6) gold inlay with GI cement, 7) composite resin only, 8) composite resin with GI cement. After develop the 3-dimensional model for finite element analysis, we observe the distribution of stress and temperature with force of 500N to apical direction at 3 point on occlusal surface and temperature of 55 degree, 15 degree on entire surface. The analyzed results were as follow : 1. Principal stress produced at the interface of base, dentin, cavity wall was smallest in case of using GI cement as base material under the amalgam. 2. Principal stress produced at the interface of base, dentin, cavity wall was smaller in case of using GI cement as a base material than ZPC under gold inlay. 3. Composite resin-filled tooth showed stress distributed over entire tooth structure. In other words, there was little concentration of stress. 4. ZOE was the most effective base material against hot stimuli under the amalgam and GI cement was the next. In case of gold inlay, GI cement was more effective than ZPC. 5. Composite resin has the small coefficient of thermal conductivity. So, composite resin filling is the most effective insulating material.

• Korean Journal of Plant Resources
• /
• v.28 no.6
• /
• pp.697-703
• /
• 2015

Twenty apple germplasm accessions from the Korean Genebank were successfully cryopreserved using two-step freezing to back up genetic resources maintained by field collections. This study examined the morphological and genetic stability of cryopreserved dormant apple buds that were stored in liquid nitrogen, and then rewarmed and regrown. Whole plants were regenerated directly from dormant buds through budding without an intermediary callus phase. The cryopreserved buds produced high levels of shoot formation (76.2-100%), similar to those of noncryopreserved buds (91.3-100%), with no observed differences between cryopreserved and noncryopreserved materials. Three of the twenty cryopreserved apple germplasm accessions were used to assess morphological and genetic stability. No differences in morphological characteristics including shoot length, leaf shape, leaf width/length ratio, and root length were observed between controls (fresh control and noncryopreserved) and cryopreserved plantlets. The genetic stability of regenerants (before and after cryopreservation) was investigated using inter simple sequence repeat (ISSR) markers. The ISSR markers produced 253 bands using four primers, ISSR 810, SSR 835, ISSR 864, and ISSR 899. These markers showed monomorphic banding patterns and revealed no polymorphism between the mother plant and regenerants before and after cryopreservation, suggesting that cryopreservation using two-step freezing does not affect the genetic stability of apple germplasm. These results show that two-step freezing cryopreservation is a practical method for long-term storage of apple germplasms.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
• /
• v.15 no.1
• /
• pp.75-83
• /
• 1985

Bitewing and periapical radiographic techniques are used in clinical and epidemiological studies to assess crestal alveolar bone levels. The purpose of the present study investigated relationships between these techniques by assessing alveolar crest location at the same site. Bitewing and periapical radiographs were available from posterior quadrants of 120 subjects. The distance from cemento-enamel junction to the alveolar crest (CEJ -CR) was measured for each proximal surface from the distal of the cuspid to the distal of the second molar. Data were arranged according to the proximal surface examined, and bitewing and periapical measurements were compared using paired tests. The obtained results were as follows; 1. In maxilla, a significant t ratio with a P value of 0.05 or less was reached for 78% and in mandible reached for 39%. 2. Pearson correlations were calculated 32 surfaces (89%) had values below 0.70 and so, data obtained by these techniques can not be used interchangeably on an individual or group basis. 3. In situations where the periapical measure was greater than the bitewing, it ranged up to 20.7% greater with a mean 9.8%. Where the bitewing was greater than periapical, it ranged up to 51. 9% greater, with a mean 18.2%. 4. The percentage difference was greater in maxillary posterior areas than in mandibular molar, premolar areas. A lower prevalence of significant differences was attributed to relatively more simple root and favorable radiographic conditions in mandibular molar and premolar areas. 5. The anatomical limitations imposed on periapical radiographic technique, most often result in somewhat foreshortened radiographic images. This situation would tend to be accentuated by the anatomical restrictions of the hard palate. 6. Consequently, since the significant differences frequently exist between measurements obtained from bitewing and periapical techniques, it is important to define which technique is used.

• Korean Journal of Environment and Ecology
• /
• v.10 no.1
• /
• pp.49-57
• /
• 1996

To figure out the relation between the shade hours and the landscape tree growth in the apartment housing areas, the present sizes and planting positions of 4 tree species in Gwacheon-si apartment housing areas were surveyed. Then, shade hours were analyzed and the data were analyzed by simple linear regression method. As a whole, the R$^{2}$ was too low to generalize the regression equation. Therefore, it was presumed that the gravity of shade hours in landscape tree growth in this sample site was relatively lower than that of any other environmental factors. However, it was presumed that the characteristics of shade intolerant and tolerant tree were turned up, because Pinus strobus showed a low negative correlation with shade housm and Acer palmatum and Magnolia denudata showed a low positive correlation with shade hours generally. And, it was proved that the statistically significant cases were the tree diameter at root collar and tree sidth of Acer palmatum and tree width of Magnolia denudata with shade hours showing a low correlation coefficient less than 0.4.

• Journal of rehabilitation welfare engineering & assistive technology
• /
• v.10 no.3
• /
• pp.199-206
• /
• 2016

Most Koreans walk having their toes in or out, because of their sedentary lifestyles. In addition, using smartphone while walking makes having a desirable walking posture even more difficult. The goal of this study is to make a simple system which easily analyze and inform any person his or her personal walking habit. To discriminate gait patterns, we developed a gait monitoring system using a 3-axis accelerometer and a foot pressure monitoring system. The developed system, with an accelerometer and a few pressure sensors, can acquire subject's foot pressure and how tilted his or her torso is. We analyzed the relationship between type of gate and sensor data using this information. As the result of analysis, we could find out that statistical parameters like standard deviation and root mean square are good for discriminating among torso postures, and k-nearest neighbor algorithm is good at clustering gait patterns. The developed system is expected to be applicable to medical or athletic fields at a low price.