• 제목/요약/키워드: silkworm larvae

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A Microsporidian Parasite - $Lb_{ms}$ (Protozoa - Microspora) Infecting Lamerin Breed of the Silkworm Bombyx mori L. (Lepidoptera - Bombycidae)

  • Bhat Shabir Ahmad;Nataraju B.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제12권1호
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    • pp.41-43
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    • 2006
  • Lamerin breed of the silkworm Bombyx mori L. is the only mulberry silkworm breed which survives with an associated microsporidian $(Lb_{ms})$ infection from generations. This present comparative investigation has produced information on the influence of $Lb_{ms}$ on the growth and development of the breed. The $Lb_{ms}$ transmits tranovarially as well as perorally at low level. Larvae developed from the eggs laid by female, infected with $Lb_{ms}$ were as normal as larvae developed from the eggs laid by healthy female. In infected tissues early developmental stages and mature spores located intracellularly but did not show hypertrophy of cells of the host. The meronts and sporonts measures $0.46{\pm}0.25,\;1.11{\pm}0.05{\mu}m$ in length and $0.32{\pm}0.05,\;0.78{\pm}0.10{\mu}m$ in width respectively. The mature spore measures $4.36{\pm}0.06{\mu}m$ in length and $2.14{\pm}0.01{\mu}m$ in width.

Effect of rearing season, host plants and their interaction on economical traits of tropical tasar silkworm, Antheraea mylitta Drury- an overview

  • Bhatia, Narendra Kumar;Yousuf, Mohammad
    • International Journal of Industrial Entomology and Biomaterials
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    • 제29권1호
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    • pp.93-119
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    • 2014
  • Tropical tasar silkworm, Antheraea mylitta (Lepidoptera: Saturniidae) is a polyphagous silk producing forest silkworm of commercial importance in India. Forest dependent people rear its larvae on different forestry host plants twice or thrice in a year for small household income. Larvae of A. mylitta feeds on many forest tree species, but always show a great degree of selectivity as a function of its behavioural responses to physical structure and chemical features of the host plants. Cocoon crop of A. mylitta is influenced by heterogeneity of tasar food plants and climatic conditions of the habitat. The role of host plants, temperature, humidity, rainfall, photoperiod and climatic variables on the growth and development of insects have clearly been demonstrated. This article entails an in-depth analysis on ecological and nutritional aspects of A. mylitta, which may provide selective information to researcher and forest managers, who are particularly associated with livelihood improvement of the poor people in forested area through location specific forest insect industry.

NADP-Dependent Malate Dehydrogenase Activity and Associated Biometabolic Changes in Hemolyinph and Fat Body Tissues of Silkworm Bombyx mori L. Following Baculovirus Infection

  • Krishnan, N.;Chaudhuri, A.;Sengupta, A.K.;Chandra, A.K.;Sen, S.K.;Saratchandra, B.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제2권2호
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    • pp.149-153
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    • 2001
  • The influence of baculovirus Bombyx mori Nuclear Polyhedrosis virus (BmNPV) infection on intermediary metabolic pathways in silkworm Bombyx mori L. was investigated. Studies revealed that NADP-linked malate dehydrogenase activity in hemolymph of infected silkworms at 96 hrs post infection (p.i.) with visible symptoms of infection was enhanced in comparison to healthy larvae of the same age. Also, NADP-dependent MDH activity was significantly lower in fat body cytosol of infected larvae at 96 hrs p.i. when compared to healthy larvae. Similarly, some biometabolic parameters like growth, protein content and cholesterol titer were observed to be influenced by baculovirus infection. While the growth of infected larvae was significantly retardedi protein content was also drastically reduced in both hemolymph and fat body tissues. Cholesterol titers however, was enhanced in infected larvae. The results observed herein point to a significant change in the normal biochemical and biometabolic pathways required for growth and development following BmNPV infection.

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Screening of mulberry varieties for larval growth, cocoon yield, and quality through silkworm rearing

  • Merlita A. Dacayanan;Crestilyn N. Damasco;Jarson P. Libunao;Mario B. Sampaga;Edna A. Galano
    • International Journal of Industrial Entomology and Biomaterials
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    • 제46권1호
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    • pp.1-8
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    • 2023
  • Mulberry (Morus spp.) production is a vital activity in sericulture along with rearing of silkworms (Bombyx mori L.) for silk production. The Sericulture Research and Development Institute (SRDI) conducted a study that evaluated 11 mulberry varieties, including nine newly-evolved mulberry hybrids and two check varieties. These mulberry varieties were fed to silkworm hybrid, DMMMSU 115 x 100. The rearing performance of DMMMSU 115 x 100 was determined based on the silkworm economic traits such as weight of 10 matured larvae, cocoon weight, shell weight, shell percentage, cocoon yield, reelability percentage, filament length and filament size. Six silkworm rearing trials were conducted with 50 silkworm larvae per treatment in three replicates. The data were analyzed in a one-way Analysis of Variance. Significant differences were tested further using Tukey's Honest Significant Difference. Mulberry varieties that garnered an evaluation index value of higher than 50 were identified and selected. Results of the study revealed significant variations among the mulberry varieties based on the parameters gathered except on the reelability of cocoons, filament length and size. Matured silkworm larvae fed with Alf 55 were significantly heavier and higher cocoon yield compared to those fed with Alfonso and S54. Alf 32, Alf 55, Alf 58, Bat 37 and Bat 73 were identified as potential varieties and highly recommended as feed for the silkworm hybrid, DMMMSU 115 x DMMMSU 100.

가잠유충의 행동반응성에 관한 연구 II. 행동반경에 대한 품종간 차이 (Studies on the Behavioral Response in Silkworm Larvae, Bombyx mori II. Strain Difference on Walking Distance)

  • 박연규
    • 한국잠사곤충학회지
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    • 제29권2호
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    • pp.15-23
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    • 1987
  • 경상남도잠종장에서 보유하고 있는 보유잠품종 23개 품종과 1986년 현재 우리나라 장려잠품종으로 지정되어 있는 16개 원종을 합한 39개 잠품종을 대상으로 유충의 행동반경을 조사한 결과는 다음과 같다. 1. 잠아의 행동반경은 품종간 차이가 현저하였으며 유충의 각령기에 따른 평균행동은 숙잠(9.24cm)이 가장 컸고 다음은 의잠(2.58cm)이었으며 3령(0.72cm)과 5령(1.18cm)은 극히 작았다. 2. 각 품종의 상대적 행동반경의 크기는 각 령기와 더불어 항상 일정하지는 않았다. 3. 1령, 3령 및 5령기에 있어서 각 품종의 계통별 행동반경의 분포범위는 유럽종계통의 경우 큰 것에서부터 작은 것에 이르기까지 폭넓게 분포하였으며 중국종계통은 그 범위가 좁았고 일본종계통은 그 중간이었다. 한편 숙잠기의 경우는 계통간 그 차이가 뚜렷하지 않았다.

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누에 전염성 연화병의 발병 억제제에 의한 방제 (Inhibitory Effect of Guanidine Hydrochloride on the Infectious Flacherie Virus of the Silkworm, Bombyx mori)

  • 강석권;김근영;이재창;조용섭
    • 한국잠사곤충학회지
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    • 제25권2호
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    • pp.37-43
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    • 1984
  • 누에병 중에 피해가 막대한 전염성 연화병을 방제하기 위하여 바이러스 증식을 특이적으로 억제하는 guanidine 유도체중에서 그 억제효과가 큰 guanidine hydrochloride(GH)를 도입하여 전염성 연화병 바이러스의 증식억제효과에 관하여 조사하였다. 1. 누에에 대한 GH의 약해시험 결과에서는 GH농도 0.01% 수준 이하에서는 약해가 전혀 없었다. 2. FV 감염잠 혼입에 따른 피해조사에서는 화용비율은 무처리(대조) 89.2% (100)에 대하여 1% 감염잠혼입구 70.7%(79), 5% 감염잠 혼입구 38.4%(43)이었으며 1만두수견양은 대조구 21.3kg(100)에 대하여 1% 혼입구 16.7kg(77), 5% 혼입구 8.8kg(41)으로서 전자와 같은 경향으로 나타났다. 3. FV에 대한 GH의 발병억제효과는 GH 0.01% 농도에서 대조구에 비하여 약 10배의 억제효과가 있었다. 4. GH의 투여회수와 발병억제효과에서는 FV접종후 계속 투여하는 것이 가장 효과적이었다. 5. FV 접종후 GH투여시간에 대한 발병억제 효과는 접종과 동시에 투여는 것이 가장 효과적이었다. 6. FV의 GH에 의한 시험관내 불활화조사에서는 GH가 시험관내에서 FV를 불활화시키는 작용이 인정되지 않았다.

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Synergetic Effect of Minerals Mixture of Potassium Bromide and Nickel Sulphate on the Economic Traits of $CSR_2,\;CSR_4\;and\;CSR_2{\times}CSR_4$ Crossbreed Races of the Silkworm, Bombyx mori L.

  • Kochi S. C.;Kaliwal B. B.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제10권2호
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    • pp.107-117
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    • 2005
  • Oral supplementation of minerals mixture of potassium bromide and nickel sulphate on $CSR_2,\;CSR_4\;and\;CSR_2{\times}CSR_4$ crossbreed races with different concentrations (70, 110 and $160\;{\mu}g/ml$) were fed to the fifth instar larvae. Two of the four normal feeds per day was substituted with treated leaves fed to silkworm at fifth instar larvae. The controls were fed with the leaves sprayed with distilled water and normal leaves. The $CSR_4$ race treated larvae showed a significant increase in silkgland weight, cocooning percentage, female cocoon weight, male cocoon weight, male cocoon shell weight, male cocoon shell ratio, filament length, filament weight, denier, fecundity, number of eggs per ovariole and length of the ovariole when compared with those of the corresponding parameters of the carrier control, whereas in $CSR_2\;and\;CSR_2{\times}CSR_4$ crossbreed races showed a significant increase in larva1 weight and other parameters similar to that of $CSR_4$ race. The results suggested that the silkgland showed good response to minerals mixture of potassium bromide and nickel sulphate only in $CSR_4$ race when compared with those of other two races of the silkworm, B. mori.

$F(ab)_2$-ELISA for the Detection of Nuclear Polyhedrosis Virus of Silk-worm, Bombyx mori L.

  • Sivaprasad, V.;Nataraju, B.;Baig, M.;Samson, M.V.;Datta, R.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제6권2호
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    • pp.179-181
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    • 2003
  • $F(ab`)_2$-ELISA and direct antigen coating-ELISA (DAC-ELISA) were evaluated in the detection of purified Bombyx mori nuclear polyhedrosis virus (BmNPV) and nuclear polyhedrosis virus infection in silkworm larvae inoculated with BmNPV polyhedra. Although nanogram levels of BmNPV was detected in both DAC- and $F(ab`)_2$-ELISA, similar concentrations of antigen was detected in case of F(ab’)$_2$-ELISA even at higher dilution of antibody (up to 1 : 20 K). One hundred percent nuclear polyhedrosis infection was detected 6 hrs after inoculation in BmNPV infected silkworm larvae by $F(ab`)_2$-ELISA. On the other hand, detection of 100% infection was observed only three days after inoculation in DAC-ELISA. In this study, it was observed $F(ab`)_2$-ELISA was more sensitive than DAC-ELISA in the detection of purified BmNPV as well as nuclear polyhedrosis infection in silkworm larvae.

곤충성장조절제(IGR)가 누에의 발육생리에 미치는 영향 I. Fenoxycarb가 누에의 유충기 발육과 용화에 미치는 영향 (Effect of Insect Growth Regulators on Development and Physiology of the Silkworm, bombyx mori, L. I. Effect of fenoxycarb, juvernile hormone Analog, on larval growth, pupation and cocoon characters by oral application)

  • 김삼은;황석조
    • 한국잠사곤충학회지
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    • 제37권2호
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    • pp.121-126
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    • 1995
  • 1000배로 희석한 "인세가"9주성분 : fenoxycarb)를 분무한 뽕잎을 일정기간 누에에 섭식시킨후 각 령의 경과일수, 5령 유충무게, 화용비율, 전견중 및 견층중 등에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 1. fenoxycarb 첨식은 유충경과 일수를 연장시킨다. 특히 4령 3일 이후의 처리에서는 5령 경과일수가 20일 이상 길어지고 토사하지 못한채 모두 폐사하였다. 2. fenoxycarb 첨식은 5령 유충무게를 증가시킨다. 처리구의 유충무게 최대치는 대조구에 비해 2령 전기간 처리구에서 약 20%, 3령, 4령, 5령기의 전기간 처리구에서 각각 40%, 70%, 20% 정도 증가하였다. 3. 화용비율은 1령 처리구에서는 90% 이상으로 대조와 큰 차이는 없었으나 2~3령 처리구에서는 70~8-0%, 4령 후반기 이후 처리구에서는 0%였다. 4. 전견중 및 견층중은 1~3령 처리구에서 대체로 증가하였고 특히 전견중은 2령 전령 처리구에서 대조대비 47% 견층중은 3령 전기간 처리구에서 41% 증가하였다.41% 증가하였다.

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Effect of Testosterone Propionate on the Economic Traits of the Silkworm, Bombyx mori L.

  • Magadum, V.B.;Magadum, S.B.
    • 한국잠사곤충학회지
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    • 제35권1호
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    • pp.69-72
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    • 1993
  • Testosterone Propionate가 가잠의 실용형질에 미치는 영향 다화성계통의 1종인 Pure Mysore 품종에 대한 testosterone propionate(TP)의 농도별 (1, 5, 10$\mu$g/ml) 투여효과를 조사하였다. TP의 처리는 유충 3, 4, 5령의 36시간째 투여구(처리-1), 4, 5령의 48시간째 투여구(처리-2) 및 5령 72시간째 투여구(처리-3)의 3개 시험구를 설정하여 농도별로 피부에 침투하였다. 실험 결과 모든 시험구에서 TP 처리는 산란성에 증대효과를 보였으나, 화용비율 및 발아비율은 대조구에 비해 떨어지고 있었다.

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