• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.719-722
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• 2001

Silk proteins from Nephila clavipes are fibrous proteins containing repetitive sequences with both crystalline and amorphous domains. In order to obtain high-level production of silk protein, the synthetic genes had 16 contiguous units of the consensus repeat sequence of the silk protein were expressed in Escherichia coli BL21(DE3) under the strong inducible T7 promoter. For production of recombinant silk protein in large amounts, pH-stat fed-batch cultures were carried out. The recombinant silk protein was produced as soluble forms in E. coli, and the recombinant silk protein content was as high as 11% of the total protein. When cells were induced at $OD_{600}$ of 60, the amount of silk protein produced was 6.49 g/L. After simple purification steps, 9.2 mg of silk protein that was more than 80% pure was obtained from a 50 mL culture, and the recovery yield was 26.3%.

• Journal of Sericultural and Entomological Science
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• v.42 no.1
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• pp.42-47
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• 2000

Structural and thermal characteristics of silk fibroin/poly(2-hydroxyethyl methacrylate)(PHEMA) blend films were investigated using FTIR, X-ray diffractometer, differential scanning calorimeter, thermogravimeter and scanning electron microscope. FTIR spectra showed that the conformation of silk fibroin prepared by dissolving in formic acid was $\beta$-sheet, which did not affected by blending with PHEMA. The X-ray diffraction patterns also showed that individual crystalline structure of silk fibroin and PHEMA was not affected for the blend films. The initial thermal decomposition temperature of silk fibroin/PHEMA blend film tends to be higher than either of silk fkbroin or PHEMA. Thermal stability of both polymers, more notably PHEMA, can be improved by blending two components. As a result of SEM observation, the phases separation for silk fibroin/PHEMA blend films occured regardless of blend ratio ; continuous and dispersed phase were silk fibroin and PHEMA component, respectively.

• Fashion & Textile Research Journal
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• v.3 no.1
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• pp.48-52
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• 2001

The study was conducted to find out suitable flame proofing on silk fabrics and to examine closely the flame retardancy effect and the physical properties changes of the silk, which was dyed by natural dyes and synthetic dyes, treated with agent (Diphenylbutylamidophosphate (DPBAP)). The results of the study were as follows: 1) Silk could be treated with DPBAP easily soluble in water by means of simple Pad-Dry-Cure. 2) The add-on of silk fabrics dyed by natural dyes was more than that of silk fabrics dyed by synthetic dyes. 3) The silk fabrics dyed by india ink among natural dyes has more flame retardancy effect in before treating with flame proofing agent than in after treating with it. 4) The physical properties (stiffness and tensile strength) of the silk fabrics treated with flame retardancy agent were little changed.

• Journal of Sericultural and Entomological Science
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• v.40 no.2
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• pp.131-135
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• 1998

Changes of silk fibroin molecular weight was studied by enzymatic proteolysis and reverse reaction of enzymatic proteolysis (plastein reaction) using chromatography, X-ray diffraction and thermal analysis methods. When the treatment of enzymatic proteolysis with $\alpha$-chymotripsin to silk fibroin solution, a precipitate of Fcp fractions was formed. And, this was dissolved in LiBr aqueous solution, the precipitate of PIFcp fractions was obtained again. Fcp and PIFcp fractions showed silk IIand silk Itype structure, respectively. Fcp fractions was about 6,900 in molecular weight, PIFcp fractions obtained by plastein reaction on the precipitate of Fcp fractions increased molecular weight to abort 15,000. The molecular weight of Fcp fractions was increased by plastein reaction, but Fcp fractions almost transited to silk I type crystal. The structure of silk I type of PIFcp fractions was steady identified by X-ray diffraction and thermal analysis. As molecular weight of Fcp fractions was gradually low, PIFcp fractions was become to macromolecule little by little.

• Journal of the Korean Society of Costume
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• v.56 no.1 s.100
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• pp.32-43
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• 2006

Excavated fabrics from Papyeung-yun's tomb totals at 208 in their numbers. They comprised 118 pieces of silk tabby(紬), 16 pieces of thin silk tabby, 8 pieces of thin filament silk tabby (絹), 43 pieces of satin damask(段), 8 pieces of compound satin(金線), 1 piece of twill(綾), 1 piece of complex gauze(羅), l piece of simple gauze(紗), 4 pieces of cotton(綿布), 4 pieces of hemp(麻布) and 3 pieces of ramie(苧布). As shown in the preceding list, $95\%$ of all the items are weaved from silk. To list a few characteristics of excavated fabrics from Papyoung-Yun's lineage, there are A vivid gold-colored compound satin, a uniquely patterned satin damask, various silk tabbies, and 4-end complex gauze of Neoul that is women's head cover. To note, names of the fabrics used in this article are based on the information from the literatures and are as following silk tabby(紬), thin silk tabby, filament silk tabby(絹), satin damask(段), compound satin(金線), complex gauze(羅), simple gauze(紗), cotton(綿布), twill(綾), hemp(麻布)and ramie(苧布).

• Acta Via Serica
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• v.1 no.1
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• pp.119-135
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• 2016

The initial phase of the Mongol invasion resulted in the establishment of relative political stability in the vast expanses of Eurasia, which came under the control of a single political entity - the Mongol realm. This contributed to a fairly rapid restoration of the commercial links and trade routes between the East and the West. During this period, Chinese silk again became available in large quantities in the Western markets. At the same time, the beginning of silk production and manufacturing of silk fabrics in Italy and the fashion flash for these goods in Western countries affected trade between Europe and the Muslim world. The centers of silk production in the Ilkhanid Empire were some provinces of Azerbaijan and Persia, where from it was exported in large numbers along the trade routes of Anatolia and Syria to the Mediterranean ports and further to the west. There are numerous testimonies of European travelers, and Muslim authors related the international silk trade in 13th-15th centuries, ie in the era from the Mongol Ilkhanid Empire till the reign of the Turkman Aqqoyunlu dynasty. One of the most informative documentary sources on this issue are the legislative codes (kanuname) of sultan Uzun Hasan from the Aqqoyunlu dynasty regarding the eastern provinces of the Asia Minor. This article presents and analyzes the information from these documents concerning the whole range of goods related to silk and silk fabrics trade in the period under the consideration.

• Textile Coloration and Finishing
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• v.18 no.6 s.91
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• pp.37-42
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• 2006

Researches to preserve and restore the remaining fabrics as costume heritages have been carried out. In this study, in order to artificially restore an excavated silk fabrics, degummed silk fabrics and safflower dyed silk fabrics were prepared for an experiment. These fabrics were photo-degraded by the Xenon arc beam to have various strength retention(100%, 80%, 60%, 40%, 20%). The fine structure and physical properties of Xenon arc treated fabrics were investigated with various techniques such as tensile test, weight loss, wide-angle X-ray diffraction, yellowness, color, SEM etc. Tensile strength and the crystal diffraction intensity of silk fabrics decreased as Xenon arc hem treatment time increased. Weight loss increased slightly. Strength retention was decreased as the Xenon arc beam treatment time goes by. (Yellowness of the undyed silk fabrics and $L^*$ of the dyed silk fabrics increased. Whiteness of the undyed silk fabrics and $b^* of the dyed silk fabrics decreased.) SEM results of the silk fabrics treated Xenon arc beam show that surface was a little damaged.

• Journal of Sericultural and Entomological Science
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• v.41 no.2
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• pp.116-121
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• 1999

This study was aimed to explain the essence of Hydroxylammonium hydrochloride(H.A.) effect on degummed silk fiber increasing the colour sites due to oxime generating reaction. H.A. in aqueous solution caues to increase the amount of [H+] and reduce pH values as the concentration of H.A. increases. The rate of [H+] absorption of silk fiker in acidic solution differs on the basic of solution pH and shows a specific uptake in each pH, the lower the pH of solution, the higher the amount [H+] absorption. The pH of solution after treating of silk fiber in H.A. and HCl, showed more remaining [H+] in H.A. solution due to [H+] releasing under the procedure of oxime production. Also it was revealed that in higher concentration of H.A. the reaction for oxime fixation in silk fiber carried out stonger and as a result the bigger gap with acid uptake curve appeared. FT-IR analysis of silk fiber treated with H.A. revealed the creating of intermolecular H-bond at the 2,981-2.930 cm-1, which was not appeared for nontraeted silk fibers and shows H-bond between N-OH group in oue chain and C=) group in another chain of silk protein. Colourimetry of dyed silk fiber after H.A. tratment showed that the silk fiber treated with the high concentration of H.A. compare to low concentration, absorbed more dyeing molecules and so Showed less percontage of Whiteness.

• International Journal of Industrial Entomology and Biomaterials
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• v.28 no.2
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• pp.66-70
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• 2014

In order to the feasibility of silk materials as protein delivery system, silk beads incorporated with bovine serum albumin (BSA) were prepared by dropping silk fibroin extract into dope solution composed of ethanol and dichloromethane. Structural and morphological characteristics of silk beads were examined using scanning electron microscopy (SEM), infrared spectrometry, and X-ray diffractometry. Swelling ratio of silk beads was also measured. Release behavior of prototypical protein, BSA, was studied by observing the electropheretic phenomenon and release profile. SEM showed that silk beads are spherical with porous interior structure. Infrared spectrometry and X-ray diffraction confirm that the silk beads have a ${\beta}$-sheet conformation. The swelling capability of silk beads increased with the incorporation of the protein. The protein was released from the beads with slow release following an initial burst release. Therefore, silk beads show promise as materials for encasing protein drugs to be delivered to targets in the human body.

• International Journal of Industrial Entomology and Biomaterials
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• v.35 no.2
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• pp.83-88
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• 2017

Silk is appealing materials for many biomedical applications involving tissue engineering and implantable devices, because of its biocompatibility, environmental stability, controlled proteolytic biodegradability and morphologic flexibility. Silk matrix is required for the treatment of a wide wound area, but the present silk matrix is made by the second processing, and thus, the labor and the cost are high. In this work, we investigated the optimal production condition of natural silk 3D matrix using the silkworms and invented Automatic Silk Matrix Making Machine (ASMMM) for natural silk 3D matrix production. As a result, we determined that optimal production condition for making A4 paper size natural silk 3D matrix was used Rough aquarelle paper on surface at $25^{\circ}C$ and 30 silkworm larvae. These results are expected to provide basic data for the efficient production of the natural silk 3D matrix, and it is suggested that the produced natural silk 3D matrix is useful as a medical biomaterials.