• Title/Summary/Keyword: silica-gel

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Lupane Triterpenoids from Pyrus pyrifolia

  • Yoo, Ji-Hye;Yang, Ki-Sook
    • Natural Product Sciences
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    • v.18 no.1
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    • pp.13-15
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    • 2012
  • Three lupane type triterpenoids were isolated from the methanol extract of Pyrus pyrifolia fruit peel through repeated silica gel column chromatography. Based on the spectroscopic methods, their structures were determined to be lupeol (1), betulin (2), and betulinic acid (3).

The Structure and Antibiotic Activities of Hydroxy Acid of Lanostenol Compound in Daedalea dickinsii

  • Bae, Gang Gyu;Min, Tae Jin
    • Bulletin of the Korean Chemical Society
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    • v.21 no.12
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    • pp.1199-1201
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    • 2000
  • 31-Hydroxycarbowyacetylquercinic acid, a lanostenoid hydroxyl acid has been isolated from D. dickinsii by solbent extraction, silica gel column chromatography and recrystallization. The structure of this compound has been determined to be 31-hydroxycarboxyacetylquercinic acid by a combinationof spectral data and by HM-BC. This compound showed antimicrobial activities against human pathogenic fungi and bacteria.

Isolation and Identification of Antimicrobial Compounds from Licorice Extracts (감초 추출물로부터 항균성 물질의 분리 및 동정)

  • Lee, Jin-Man;Lee, Yoon-Won;Hur, Sang-Sun
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.2
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    • pp.255-263
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    • 2016
  • Antimicrobial properties of Licorice(Glycyrrhizae radix L.) against food spoilage microorganism, Bacillus subtilis KCTC 1021 was investigated. Antibacterial activity of the essential oil was as equivalent as Potassium metabisulfite and myconazole. The licorice extracts was fractionated to hexane, chloroform, ethyl acetate, butanol and water fraction. Chloroform fraction showed the highest inhibitory effect on the Bacillus subtilis KCTC 1021. Chloroform fraction was further fractionated by silica gel column chromatography and thin layer chromatography(TLC). The antibacterial compound was isolated from their fractions and its chemical structures was identified as (R)-glabridin by ESI-MS, $^1H$-NMR and $^{13}C$-NMR.

A Nucleoside with Lipid Peroxidation Inhibitory Activity from Agrocybe cylindracea. (버들송이로부터 분리한 Nucleoside계 화합물의 지질과산화 저해활성)

  • 이인경;윤봉식;유익동
    • Microbiology and Biotechnology Letters
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    • v.26 no.6
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    • pp.558-561
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    • 1998
  • In the screening for lipid peroxidation inhibitors from edible mushroom, Agrocybe cylindracea, a bioactive compound AG 8 was isolated. The AG 8 was purified from methanol extract of its fruit body by Diaion HP-20 column chromatography, ethyl acetate extraction, and silica gel column chromatography, consecutively. Based on various NMR studies including $^1$H irradiation and HMBC experiments, the AG 8 was identified as MTA, 5'-deoxy-5'-methylthioadenosine. This compound inhibited lipid peroxidation with an $IC_{50}$/ value of 3.2 $\mu\textrm{g}$/$m\ell$. The MTA was isolated for the first time from basidiomycetes.

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