• Animal cells and systems
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• v.12 no.3
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• pp.149-155
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• 2008

We have isolated and characterized Agrius convolvuli cDNA encoding a c-type lysozyme. The cDNA sequence encodes a processed protein of 139 amino acid residues with 19 amino acid residues amino-terminal signal sequence and 120 amino acid residues mature sequence. The amino acid residues responsible for the catalytic activity and the binding of the substrate are conserved. Agrius lysozyme has a high identity to Manduca sexta. Recombinant A. convolvuli lysozyme was expressed in Escherichia coli BL21(DE3) pLysS cells for pGEX 4T-1 expression vector. Their optimal conditions for the fusion protein expression and purification were screened. Lysozyme gene amplified with primers ACLyz BamHI and ACLyz XhoI was ligated into the pGEX 4T-1 vector, which contained the glutathione S-transferase(GST) gene for fusion partner. The fusion protein was induced by IPTG and identified by SDS-PAGE analysis. Molecular weight of the fusion protein was estimated to be about 45 kDa. Recombinant lysozyme, fused to GST, was purified by glutathion-Sepharose 4B affinity chromatography. Western blot analysis of this protein revealed an immunoreactivity with the anti-Agrius lysozyme.

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1525-1535
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• 2014

The xynC gene, which encodes high molecular weight xylanase from Paenibacillus sp. DG-22, was cloned and expressed in Escherichia coli, and its nucleotide sequence was determined. The xynC gene comprised a 4,419bp open reading frame encoding 1,472 amino acid residues, including a 27 amino acid signal sequence. Sequence analysis indicated that XynC is a multidomain enzyme composed of two family 4_9 carbohydrate-binding modules (CBMs), a catalytic domain of family 10 glycosyl hydrolases, a family 9 CBM, and three S-layer homologous domains. Recombinant XynC was purified to homogeneity by heat treatment, followed by Avicel affinity chromatography. SDS-PAGE and zymogram analysis of the purified enzyme identified three active truncated xylanase species. Protein sequencing of these truncated proteins showed that all had identical N-terminal sequences. In the protein characterization, recombinant XynC exhibited optimal activity at pH 6.5 and $65^{\circ}C$ and remained stable at neutral to alkaline pH (pH 6.0-10.0). The xylanase activity of recombinant XynC was strongly inhibited by 1 mM $Cu^{2+}$ and $Hg^{2+}$, whereas it was noticeably enhanced by 10 mM dithiothreitol. The enzyme exhibited strong activity towards xylans, including beechwood xylan and arabinoxylan, whereas it showed no cellulase activity. The hydrolyzed product patterns of birchwood xylan and xylooligosaccharides by thin-layer chromatography confirmed XynC as an endoxylanase.

• Investigative Magnetic Resonance Imaging
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• v.13 no.1
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• pp.9-14
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• 2009

T1-, and T2-weighted imagings and FLAIR (fluid attenuated inversion recovery) imaging are fundamental imaging methods in the brain. T1-weighted imaging is a spin-echo sequence with short TR and short TE and produces the tissue contrast by different T1 relaxation times. In other words, short TR maximizes the difference of the longituidinal magnetization recovery between the tissues. T2-weighted imaging is a spin-echo sequence with long TR and long TE and produces the tissue contrast by different T2 relaxation times. Long TE maximizes the difference of the transverse magnetization decay between the tissues. FLAIR is an inversion recovery sequence using 180 degree inversion pulse. 2500 msec of inversion time is applied to suppress the CSF signal.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.12
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• pp.117-126
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• 2005

In this paper, we present a new method for the reduction of the PAPR (peak to average power ratio) of OFDM signals. The idea behind the proposed method is that IFFT is implemented often with software for a digital signal processor such that we may avoid the repeated calculations to reduce the computational operations: we define sub-sequences in the IFFT process and then multiply the optimum phase rotation factors to them to minimize the PAPR. The PAPR reduction performance of the proposed method is equal to that of the interleaved partition scheme of the PTS (partial transmit sequence) method with only 1/3 computational operations of it.

• Journal of the Korean Institute of Intelligent Systems
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• v.25 no.2
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• pp.139-146
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• 2015

This paper presents the solution to model matching of switched asynchronous sequential machines by corrective control. We propose a model of switched asynchronous sequential machines, in which the system can have different dynamics of asynchronous machines governed by a pre-determined sequence of switching. The control objective is to derive a corrective control law so that the stable state behavior of the closed-loop system can match that of a prescribed model. A new skeleton matrix is defined to represent the reachability of the switched asynchronous machine, and a novel control scheme is presented that interweaves the switching signal and the corrective control procedure. A design algorithm for the proposed controller is illustrated in a case study.

• Journal of Communications and Networks
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• v.8 no.3
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• pp.267-274
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• 2006

Self-spreading (SSP) is a spread spectrum technique where the spreading sequence is generated from data bits. Although SSP allows communications with low probability of interception by unintended receivers, despreading by the intended receiver is prone to error propagation. In this paper, we propose both a new transmitter and a new receiver based on SSP with the aim to a) reduce error propagation and b) increase the concealment of the transmission. We first describe a new technique for the generation of SSP spreading sequence, which generalizes SSPs of existing literature. We include also coding at the transmitter, in order to further reduce the effects of error propagation at the receiver. For the receiver, we propose a turbo architecture based on the exchange of information between a soft despreader and a soft-input soft-output decoder. We design the despreader in order to fully exploit the information provided by the decoder. Lastly, we propose a chip decoder that extracts the information on data bits contained in the spreading sequence from the received signal. The performance of the proposed scheme is evaluated and compared with existing spread-spectrum systems.

• Journal of Korea Foundry Society
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• v.20 no.2
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• pp.97-103
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• 2000

In this study, a design of experiment, Taguchi method, was applied to optimize gating system design of escalator step die casting parts. Six shape factors which affect filling sequence of melt are adopted and divided into two levels respectively. Initial feeding differences of melt which were calculated by using S/N(signal-to-noise) ratio in each condition were demonstrated with the simulation of Flow-3D software program. Variations of S/N ratio according to shape factors were obtained and the optimal condition of gating system could also be obtained. It could be found that width of gate, contact angle of gate, thickness of runner are more effective factors on the filling sequence of melt than the others in this case of escalator step die casting parts. It showed that the economical gating system and sound filling sequence of melt were obtained by using Taguchi method.

• International Journal of Industrial Entomology and Biomaterials
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• v.21 no.1
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• pp.127-132
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• 2010

We have characterized full-length cDNA encoding Gall-6-tox protein, which was cloned from the fat body of the immunized Galleria mellonella larvae. The cloned cDNA of Gall-6-tox consists of 1301 nucleotides and contained an open reading frame of 891 nucleotides corresponding to a protein of 296 residues that includes a putative 16-residue signal sequence and a 280-residue mature peptide with a calculated mass of 30,707.73 Da. The deduced mature peptide contains conserved tandem repeats of six cysteine-stabilized alpha beta ($Cs{\alpha}{\beta}$) motifs, which was detected in scorpion toxins and insect defensins. In the sequence homology search, mature Gall-6-tox showed 34% and 28% amino acid sequence homology with Bomb-6-tox from Bombyx mori and Spod-11-tox from Spodoptera frugiperda, respectively. Gall-6-tox orthologs were only found in Lepidopteran species, indicating that this new immune-related gene family is specific to this insect order. RT-PCR analysis revealed that Gall-6-tox was expressed primarily in the larval fat bodies, hemocytes, and midgut against invading bacteria into hemocoel. Moreover, the expression time course of Gall-6-tox was examined up to 24 h in the fat bodies and midgut after injection of E. coli. Altogether, these results suggest that Gall-6-tox is derived from defensins and Gall-6-tox may play a critical role in Lepidoptera immune system.

• The Journal of the Acoustical Society of Korea
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• v.28 no.3
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• pp.208-212
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• 2009

Ground wave is an acoustic wave propagating at a sediment sound speed in the case that sediment sound speed is constant with depth, which is explained by modal dispersion effects. In this paper, the ground wave in time domain is simulated using the ray-based approach, which is possible because the modal dispersion can be explained by the guiding of energy caused by reflection and refraction in the waveguide geometry. For a Pekeris waveguide, the ground wave can be interpreted as a sequence of head waves, called a head wave sequence [Choi and Dahl, J. Acoust. Soc. Am. 119, 3660-3668 (2006)]. The ground wave is simulated by convolution of the source signal with a channel impulse response of the head wave sequence, which is compared with simulated signals obtained via a Fourier synthesis of a complex parabolic equation (PE) field.

• Journal of IKEEE
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• v.13 no.1
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• pp.65-70
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• 2009

We propose an ultrashort pulse optical code-division multiple-access (O-CDMA) scheme based on a pseudorandom binary M-sequence spectral phase encoding and decoding of coherent mode-locked laser pulses and perform a numerical simulation to analyze its feasibility. We demonstrate the ability to properly decode any of the multiple (eight) 10 Gbit/s users by the matched code selection of the spectral phase decoder. The peak power signal to noise ratio of properly and improperly decoded $8{\times}10 Gb/s$ signals could be greater than 15 for 127 M-sequence coding.