• Journal of the Institute of Convergence Signal Processing
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• v.10 no.1
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• pp.13-22
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• 2009

In this paper, we propose the 3D shape reconstruction method that combine the mosaic method and the active stereo matching using the laser beam. The active stereo matching method detects the position information of the irradiated laser beam on object by analyzing the color and brightness variation of left and right image, and acquires the depth information in epipolar line. The mosaic method extracts feature point of image by using harris comer detection and matches the same keypoint between the sequence of images using the keypoint descriptor index method and infers correlation between the sequence of images. The depth information of the sequence image was calculated by the active stereo matching and the mosaic method. The merged depth information was reconstructed to the 3D shape information by wrapping and blending with image color and texture. The proposed reconstruction method could acquire strong the 3D distance information, and overcome constraint of place and distance etc, by using laser slit beam and stereo camera.

• Journal of the Institute of Convergence Signal Processing
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• v.5 no.4
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• pp.294-300
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• 2004

According to the development of digital media technologies various algorithms for video clip matching have been proposed to match the video sequences efficiently. A large number of video search methods have focused on frame-wise query, whereas a relatively few algorithms have been presented for video clip matching or video shot matching. In this paper, we propose an efficient algorithm to index the video sequences and to retrieve the sequences for video clip query. To improve the accuracy and performance of video sequence matching, we employ the Cauchy function as a similarity measure between histograms of consecutive frames, which yields a high performance compared with conventional measures. The key frames extracted from segmented video shots can be used not only for video shot clustering but also for video sequence matching or browsing, where the key frame is defined by the frame that is significantly different from the previous frames. Experimental results with color video sequences show that the proposed method yields the high matching performance and accuracy with a low computational load compared with conventional algorithms.

• Korean Journal of Plant Tissue Culture
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• v.28 no.4
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• pp.201-204
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• 2001

Acyl-CoA binding proteins (ACBP) are small highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A cDNA encoding ACBP was identified from cDNA library constructed from hairy root poly $A^{＋}$ RNA in expressed sequence tags (EST) analysis. The cDNA clone was 453 bp long and carried an open reading frame of 264 bp (10 kDa). The ginseng ACBP amino acid sequence was compared with other reported plant ACBPs using the CLUSTALW. Ginseng ACBP is 89%, 81%, 80%, and 73% identical with ACBP from castor bean, lilly, Digitalis and Arabidopsis, respectively. However, ginseng ACBP is 5 amino acids smaller than Arabidopsis and rape seed ACBPs. Also there is no any known signal peptide sequence in ginseng ACBP.

• The Journal of the Acoustical Society of Korea
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• v.41 no.1
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• pp.16-29
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• 2022

This paper presents a Doppler shift estimation method and signal processing schemes for Direct Sequence Spread Spectrum (DSSS) transmission to overcome the Doppler shift due to the moving of the underwater communication unit. The proposed method estimates a Doppler shift via 2 step procedures using the preamble with the two 64-length Frank sequences which has a good self-correlation characteristic and is insensitive to the Doppler shift. Furthermore, a packet of DSSS underwater mobile communication and a RAKE receiver are designed using the proposed Doppler shift estimation method. Due to the modulation scheme of the designed DSSS underwater mobile communication using Differential-Quadrature Phase Shift Keying (DQPSK) for the data symbol transmission, the RAKE receiver dose not need a phase tracking and easily makes coherent signals among the combining RAKE branches. The designed RAKE receiving scheme including the proposed Doppler shift estimation method successfully decides information data using the DSSS signal transmitted from the pseudo-moving transmitter with velocity upto about 17.5 m/s.

• Clinical and Experimental Vaccine Research
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• v.12 no.2
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• pp.156-171
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• 2023

Purpose: The development of vaccines that confer protection against multiple avian influenza A (AIA) virus strains is necessary to prevent the emergence of highly infectious strains that may result in more severe outbreaks. Thus, this study applied reverse vaccinology approach in strategically constructing messenger RNA (mRNA) vaccine construct against avian influenza A (mVAIA) to induce cross-protection while targeting diverse AIA virulence factors. Materials and Methods: Immunoinformatics tools and databases were utilized to identify conserved experimentally validated AIA epitopes. CD8⁺ epitopes were docked with dominant chicken major histocompatibility complexes (MHCs) to evaluate complex formation. Conserved epitopes were adjoined in the optimized mVAIA sequence for efficient expression in Gallus gallus. Signal sequence for targeted secretory expression was included. Physicochemical properties, antigenicity, toxicity, and potential cross-reactivity were assessed. The tertiary structure of its protein sequence was modeled and validated in silico to investigate the accessibility of adjoined B-cell epitope. Potential immune responses were also simulated in C-ImmSim. Results: Eighteen experimentally validated epitopes were found conserved (Shannon index <2.0) in the study. These include one B-cell (SLLTEVETPIRNEWGCR) and 17 CD8⁺ epitopes, adjoined in a single mRNA construct. The CD8⁺ epitopes docked favorably with MHC peptidebinding groove, which were further supported by the acceptable ∆G_bind (-28.45 to -40.59 kJ/mol) and Kd (<1.00) values. The incorporated Sec/SPI (secretory/signal peptidase I) cleavage site was also recognized with a high probability (0.964814). Adjoined B-cell epitope was found within the disordered and accessible regions of the vaccine. Immune simulation results projected cytokine production, lymphocyte activation, and memory cell generation after the 1st dose of mVAIA. Conclusion: Results suggest that mVAIA possesses stability, safety, and immunogenicity. In vitro and in vivo confirmation in subsequent studies are anticipated.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.11
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• pp.1539-1548
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• 2016

Beamforming technique at the ground station is known to be effective in obtaining coverage extension or SNR gain for communication with unmanned aerial vehicle (UAV). When a UAV moves, periodic beam tracking is necessary to maintain beam gain. In order to track beams for multiple UAVs, the ground station needs to receive different preamble sequences from multiple UAVs. In this paper, a preamble sequence design technique is proposed for beam tracking in a GMSK-based communication system with multiple UAVs. Hadamard sequence is considered for the design of preamble sequence due to its ideal cross-correlation property. A preamble sequence appropriate for a GMSK communication system with multiple UAVs is proposed after analyzing the properties of received signal in a GMSK system with the input of Hadamard sequence.

• Microbiology and Biotechnology Letters
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• v.35 no.4
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• pp.352-356
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• 2007

A Lactobacillus sp. has been isolated from infant feces and characterized according to its physiological properties and identified as Lactobacillus acidophilus KLA1012. A gene coding surface layer protein (SLP) has been cloned and the sequence has been determined. The nucleotide sequence of slpA was 1,338 bp in size and was identical to that of L. acidophilus ATCC 4356 (100%). Amino acid sequence of SLP-A was deduced from the nucleotide sequence and it had signal sequence at N-terminal, consisting of positively charged amino acid mainly lysine. slpA was cloned and heterologously expressed in E. coli M15 and the 45.2 kDa surface-layer protein band was examined by SDS-PAGE and confirmed by Western blotting using polyclonal antibody against L. acidophilus KLA 1012 SLP-A protein.

• Journal of Plant Biotechnology
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• v.1 no.1
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• pp.46-55
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• 1999

The expressed sequence tags(ESTs) from immature seed of rice, Oryza sativa cv Milyang 23, were partially sequenced and analyzed by homology. As of 1998, the partial sequences of about 6,600 cDNA clones were analyzed from normal and normalized immature seed cDNA libraries. About 2,200 ESTs were putatively identified by BLASTX deduced amino acid sequence homology analysis. About 20% of them were putatively identified as storage proteins. Also the clones were highly homologous to genes involved particularly in starch biosynthesis, glycolysis, signal transduction and defenses. Compared to 35% of redundancy in the ESTs of normal cDNA library, that from the substracted library was 15%. The Korea Rice Genome Network is maintained to provide the updated information of sequences, their homologies and sequence alignments of ESTs. For the stable expression of transgene in rice, diverse vectors were developed for overexpression, targeting and gene dosage effect with transit peptides (Tp) and matrix attachment region (MAR) sequence from chicken lysozyme locus. The rice calli were transformed via Agrobacterium tumefaciens LBA4404(pSB1) with the triparental mating technique and selected by herbicide resistance. The green fluorescent protein(GFP) gene in expression vector under the control of rbcS promoter-Tp was overexpressed upto 10 % of the total soluble protein. In addition, the Tp-sGFP fusion protein was properly processed during translocation into chloroplast. The expression of sGFP in the presence of MAR sequences was analyzed with Northern and immunoblot analysis. All the lines in which sGFP transgene with MAR sequence, showed position independent and copy number-dependent expression, while the lines without MAR showed the varied level of expression with the integration site. Thus the MAR sequence significantly reduced the variation in transgene expression between independent transformants.

• Journal of Broadcast Engineering
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• v.13 no.3
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• pp.350-364
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• 2008

Single frequency network (SFN) design based on the Advanced Television Systems Committee (ATSC) specification, a terrestrial digital television (DTV) system, normally causes a interference problem, among signals from multiple transmitters or repeaters. To solve this, the ATSC recommended practice (RP) introduces a transmitter identification (TxID) signal embedded in a signal from each transmitter or repeater. A TxID signal analyzer is then used to detect the TxID signal, and following the analysis results, a SFN design can be adjusted. This paper discusses the generation and usages of Kasami sequence, is used the TxID signal. The configuration of the TxID signal analyzer to efficiently detect TxID signal is proposed and the results of theoretical performance analysis are provided. Moreover, computer simulation and laboratory test results are provided to evaluate the performance of TxID signal analyzer and the theoretical performance analysis.

• International Journal of Computer Science & Network Security
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• v.24 no.4
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• pp.206-210
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• 2024

Industrial facilities that use modern IT technologies require the ensured reliability and security of information in automated enterprise management. Concurrently, so as to ensure a high quality of communication, it is necessary to expand the bandwidth of communication channels, which are limited by the physical parameters of the radio frequency spectrum. In order to overcome this contradiction, we propose the application of technology fundamental to ultra-wideband signals, in which the ratio between the bandwidth and its central part is greater than "one". For this reason, the information signal is emitted without a carrier frequency - simultaneously within the entire frequency band - provided that the signal level is lower than the noise level. For the transmission of information content, the method of positional-time coding is used, in which each information bit is encoded by hundreds of ultrashort pulses that arrive within a certain sequence. Mathematical models of signals and values observed in wireless communication systems with autocorrelation reception of modulated ultra-wideband signals are furthermore recommended. These assist in identifying features of the dependence of the error probability on the normalized signal-to-noise ratio and the signal base. Comparative analysis has shown that the best noise immunity of the systems considered in this paper is the communication system, which uses the time separation of the reference and information signals. During the first half of the bit interval, the switch closes the output of the transmitter directly to the generator of the ultra-wideband signal - forming a reference signal. In the middle of the bit interval, the switch alternates the output to one of two possible positions depending on the encoding signal - "zero" or "one", forming the information part of the ultra-wideband signal. It should also be noted that systems with autocorrelation reception and separate transmission of reference and information signals, provide a high level of structural signal secrecy. Furthermore, they provide the reliable transmission of digital information, especially in interference conditions.