• Title/Summary/Keyword: signal sequence

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3' end of putative sequences of the packaging signal in moloney-murine leukemia virus (Moloney murine Leukemia Virus에서 포장신호의 가능한 3' 끝의 염기서열)

  • 박종상
    • Korean Journal of Microbiology
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    • v.26 no.2
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    • pp.101-105
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    • 1988
  • 6M-MuLV mutants containing deldtions around the putative packaging signal were constructed by using recombinant DNA technique and transfected into NIH/3T3 cell. 2 of 6 mutants can not be packaged into virions even in the presence of the wild type helper virus. The boundary between the packagible and the non-packagible genome is located around Pvu I site, 421 nucleotide downstream from the 5' end of M-MuLV genome. 10 base pair inverted repeat sequence (GAGUCCAAAA) which can make stem structure around Pvu Isite could be the putative packaging signal.

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An Adaptive SLM Scheme Based on Peak Observation for PAPR Reduction in OFDM Systems (OFDM 시스템에서 PAPR 감소를 위한 피크 신호 관찰 기반의 적응적 SLM 기법)

  • Yang, Suck-Chel;Shin, Yo-An
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.12C
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    • pp.1142-1148
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    • 2007
  • In this paper, we propose ASLM (Adaptive Selective Mapping; ASLM) scheme based on peak observation for PAPR (Peak-to-Average Power Ratio) reduction of OFDM (Orthogonal Frequency Division Multiplexing) signals. The proposed scheme is composed of three steps: peak scaling, sequence selection, and SLM procedures. In the first step, the peak signal samples in the IFFT (Inverse Fast Fourier Transform) outputs of the original input sequence are scaled down. In the second step, the sub-carrier positions where the power difference between the original input sequence and the FFT output of the scaled signal is large, are identified. Then, the phase sequences having the maximum number of phase-reversed sequence words only for these positions are selected. Finally, the generic SLM procedure is performed by using only the selected phase sequences for the original input sequence. Simulation results show that the proposed scheme significantly reduces the complexity in terms of IFFT and PAPR calculation than the conventional SLM, while maintaining the PAPR reduction performance.

NaCl-dependent Amylase Gene From Badillus circulans F-2 Its Nucleotide Sequence (Bacillus circulans F-2의 NaCl 의존성 amylase 유전자의 DNA 염기배열 결정)

  • 김철호;권석태;타니구치하지메;마루야마요시하루
    • Microbiology and Biotechnology Letters
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    • v.18 no.3
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    • pp.309-316
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    • 1990
  • The sequence of a 1795 bp restriction fragment containing the B. circulans F-2 gene for NaC1- dependent $\alpha$-amylase (CI-amylase) is reported. The probable coding region of the gene is 1005 base pairs (335 amino acida) long. The NaC1-dependent $\alpha$-amylase (el-amy) sequence shows an open reading frame (ORF) with the translated molecular weight of about 38, 006, which correspond to a molecular weight of about 35, 000 (Mi). The gene is preceded by the sequence resembling promoter for the vegetative B, subtitis RNA polymerases. These are followed by the sequences resembling a B. subtilis ribosome binding site 5 nucleotides before the first codon of the gene. Homologous regions with other amylases were found. The N-terminal sequences of the mature proteins expressed in E. eoli were identical to the N-terminal sequences which are anaIysed.

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Nucleotide Sequence of an Extracellular Phospholipase D Gene from Streptomyces somaliensis and Transphosphatidylation Activity of Its Enzyme (Streptomyces somaliensis가 생산하는 세포외 Phospholipase D의 유전자 서열 분석과 Transphosphatidylation 활성 특성)

  • Jeong Sujin;Lee Sun-Hee;Uhm Tai-Boong
    • Korean Journal of Microbiology
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    • v.40 no.3
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    • pp.211-216
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    • 2004
  • A bacterial strain JE-ll found to produce active extracellular phospholipase D (PLD) was selected from the soil isolates. It was identified as Streptomyces somaliensis on the basis of 16S rDNA sequence analysis, morphological and physiological characteristics. The gene (sspld) encoding S. somaliensis PLD was isolated and characterized. The open reading frame was suggested to encode 538 amino acids with a signal peptide of 33 amino acids. The deduced amino acid sequence of the sspld shared a sequence similarity of 70-88% with PLDs of other Streptomyces sp. so far reported. The PLD converted phosphatidylcholine to phosphatidylglycerol or phosphatidylserine with the yield of 96 to 99% (㏖/㏖), but did not act on inositol or ethanolamine as a transphosphatidylation donor.

Molecular Cloning and Characterization of Calumenin in Rabbit Skeletal Sarcoplasmic Reticulum

  • Jung, Dai-Hyun;Kim, Do-Han
    • Proceedings of the Korean Biophysical Society Conference
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    • 2003.06a
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    • pp.53-53
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    • 2003
  • Calumenin was previously identified as a high affinity Ca$\^$2+/ binding protein in mouse cardiac sarcoplasmic reticulum (SR). For the present study, a 48 kDa skeletal homologue of calumenin was identified by sucrose-density gradient of rabbit skeletal SR membranes, concanavalin A treatment, 2D-gel electrophoresis, $\^$45/Ca$\^$2+/ overlay, Stains-all staining, and MALDI-TOF analysis. We attempted to clone the skeletal calumenin by RT-PCR based on mouse cardiac and human calumenin sequences. The deduced amino acid sequence (315 residues) of the skeletal calumenin showed high identity to mouse cardiac calumenin (90%). As seen in the cardiac calumenin, the deduced sequence contains a 19 amino acid N-terminal signal sequence and a HDEF C-terminal sequence, a putative retrieval signal to ER. Also, the skeletal calumenin contains one N-glycosylation site, three PKC phosphorylation sites, eight casein kinase 2 phosphorylation sites, and 6 EF-hand domains. GST-calumenin showed a conformational change and increased mobility in the presence of Ca$\^$2+/ in SDS-PAGE. Three calumenin interacting proteins (ryanodine receptor 1, glycogen phosphorylase, and phosphofructo kinase) were identified by pull-down assay with GST-calumenin and solubilized SR. All the interactions were Ca$\^$2+/dependent. The present results suggest that calumenin plays an important role in Ca$\^$2+/ homeostasis of muscle cells.

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Lossless Coding Scheme for Lattice Vector Quantizer Using Signal Set Partitioning Method (Signal Set Partitioning을 이용한 격자 양자화의 비 손실 부호화 기법)

  • Kim, Won-Ha
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.38 no.6
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    • pp.93-105
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    • 2001
  • In the lossless step of Lattice Vector Quantization(LVQ), the lattice codewords produced at quantization step are enumerated into radius sequence and index sequence. The radius sequence is run-length coded and then entropy coded, and the index sequence is represented by fixed length binary bits. As bit rate increases, the index bit linearly increases and deteriorates the coding performances. To reduce the index bits across the wide range of bit rates, we developed a novel lattice enumeration algorithm adopting the set partitioning method. The proposed enumeration method shifts down large index values to smaller ones and so reduces the index bits. When the proposed lossless coding scheme is applied to a wavelet based image coding, the proposed scheme achieves more than 10% at bit rates higher than 0.3 bits/pixel over the conventional lossless coding method, and yields more improvement as bit rate becomes higher.

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A Novel Calibration Method Using Zadoff-Chu Sequence and Its FPGA Implementation (Zadoff-Chu sequence를 이용한 실시간 Calibration 알고리즘과 FPGA 구현)

  • Jang, Jae Hyun;Sun, Tiefeng;Yang, Hyun Wook;Choi, Seung Won
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.9 no.3
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    • pp.59-65
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    • 2013
  • This paper presents a novel calibration method for a base station system adopting an antenna array. The proposed technique utilizes Zadoff-Chu sequence, which is included in the LTE pilot signal periodically, in order to compute the phase characteristic of each antenna channel. As the Zadoff-Chu sequence exhibits an excellent autocorrelation characteristic, it is possible for the receiving base station to retrieve the Zadoff-Chu sequence transmitted from each mobile terminal. In addition, we can obtain the phase characteristic of each antenna channel, which is the ultimate goal of the calibration procedure. The proposed calibration algorithm has been implemented using an FPGA (Field Programmable Gate Array). We have applied the proposed algorithm to an array consisting of 2 antenna elements for simplicity. the phase value implied to the first and second antenna path is very accurately calculated from the proposed procedure. From the experimental test, the proposed method provides accurate calibration results.

A Design Method of Discrete Time Learning Control System (이산시간 학습제어 시스템의 설계법)

  • 최순철
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.13 no.5
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    • pp.422-428
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    • 1988
  • An iterative learning control system is a control system which makes system outputs follow desired outputs by iterating its trials over a finite time interval. In a discrete time system, we proposed one method in which present control inputs can be obtained by a linear combination of the input sequence and time-shifted error sequence at previous trial. In contrast with a continous time learning control system which needs differential opreration of an error signal, the time shift operation of the error sequence is simpler in a computer control system and its effectiveness is shown by a simulation.

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Dummy Sequence Insertion for PAPR Reduction of OFDM Communication System (OFDM 통신시스템의 PAPR 저감을 위한 더미 시퀀스 삽입)

  • 이재은;유흥균;정영호;함영권
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.14 no.12
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    • pp.1239-1247
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    • 2003
  • OFDM(orthogonal frequency division multiplexing) communications system is very attractive for the high data rate transmission in the frequency selective lading channel. Since OFDM has high PAPR(peak-to-average power ratio), OFDM signal may be distorted by the nonlinear HPA(high power amplifier). In this paper, we propose the DSI(dummy sequence insertion) method for OFDM communication system. Some sub-carriers are inserted for PAPR reduction. They carry the specified dummy data sequence which are used for only PAPR reduction and do not work as side information like the conventional PTS(partial transmit sequence) or SLM(selected mapping) method. We use the complementary sequence and the combination of the correlation sequence as the dummy sequence. Flipping technique is used for the DSI method to get the effective PAPR reduction. It is important that BER of the proposed method is independent of the damage of the dummy data sequence. And DSI method has better spectral efficiency than the conventional block coding. On the other hand, threshold PAPR method is applied to cut down the processing time. However, this DSI method is not better than the conventional PTS method in the respect of the PAPR reduction performance. The DSI method includes the threshold PAPR lower than the PAPR of the OFDM signal, reduces the processing time and improves the BER performance.

An Analysis of Optimal Sequences for the Detection of Wake-up Signal in Disaster-preventing Broadcast (재난방송용 대기모드 해제신호 검출을 위한 최적 부호 성능 분석)

  • Park, Hae Yong;Jo, Bonggyun;Kim, Heung Mook;Han, Dong Seog
    • Journal of Broadcast Engineering
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    • v.19 no.4
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    • pp.491-501
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    • 2014
  • Recently, the need for disaster-preventing broadcast has increased gradually to cope with natural disaster like earthquake and tsunami causing enormous losses of both life and property. In disaster-preventing broadcast system, the wake-up signal is used to alert user terminal and switch the current state of channel to the emergency channel, which is for the fast and efficient delivery of emergency information. In this paper, we propose the detection method of wake-up signal for disaster-preventing broadcast systems. The wake-up signals for disaster-preventing broadcast should have a good auto-correlation property in low power and narrow-band conditions that does not affect the existing digital television (DTV) system. The suitability of the m-sequence and complementary code (CC) is analyzed for wake-up signals according to signal to noise ratio. A wake-up signal is proposed by combining the direct sequence spread spectrum (DSSS) technique and pseudo noise (PN) sequences such as Barker and Walsh-Hadamard codes. By using the proposed method, a higher detecting performance can be achieved by the spreading gain compared to the single long m-sequence and the Golay code.