• Journal of Plant Biotechnology
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• v.6 no.4
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• pp.221-227
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• 2004

Protocol was established for production of virus free healthy seeds using meristem ($0.3-0.5\;\cal{mm}$ in size) culture and field management under net house condition in tomato. The isolated meristem was found well established in MS liquid medium containing $0.1\;\cal{mg}\;1^{-1}\;of\;GA_3$. For shoot and root development either from primary meristem or from nodal segment of meristem derived plants, semisolid MS medium having $0.5\;\cal{mg}\;1^{-1}$ of IBA was found most effective. The elimination of the studied viruses (ToMV, CMV, ToLCV) in meristem-derived plants was confirmed by DAS-ELISA test. For field management of the virus eradicated meristem-derived plants, use of net house was found very effective measures to check viral vector visit and eventually infection. The meristem-derived plants were vigor and high yielder than the native seed derived plants and produced healthy seeds. Due to stop vector visit, no viral symptoms were observed in both $R_1\;and\;R_2$ plants cultivated in net house condition. Starting of viral infestation was observed in $R_2$ generation when they were planted in open house condition without control of vector visit. Therefore, for management of viral diseases, use of virus free meristem derived plantlets and their subsequent cultivation in soil under net house condition without using any vector killing insecticide can be recommended for producing healthy seeds in tomato. The developed protocol for environmentally healthy tomato seed production in Bangladesh may be used in the countries having similar tropical like environment conducive for viral vector visit.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.37-37
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• 2017

Mechanistic studies of halophytes are urgent areas of agricultural research due to the increase in saline-contaminated and irrigated land worldwide. The halophyte Suaeda glauca (S. glauca) has advantages in terms of biomass and saline elimination due to its large mass and well-developed phenotype on seashores, although its mechanistic features and growing specificities still require systematic investigation. In this study, S. glauca was cultivated under various saline concentrations (0-400 mM) in Hoagland's solution in the absence or presence of indole derivatives to elucidate physiological features. The results confirmed the optimal growth and development of S. glauca in 50 mM NaCl, and morphologies such as the number of branches, shoot length, and fresh and dry weights were improved by indole-3-carboxylic acid (ICA) treatment. The cation concentrations in roots, shoots and leaves were investigated to examine the ionic imbalances in response to saline treatment, and the results demonstrated that sodium ions accumulated to high concentrations in leaves. The levels of calcium and potassium ions in roots were maintained or slightly decreased in the presence of 50 mM NaCl and proline concentration was increased significantly in roots at optimal concentrations. These results demonstrate that the concentrations of ions and metabolites are key regulators of optimal growth by regulating the physiology of halophytes.

• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.401-408
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• 2017

Herein, we report the meristem-tip culture from dormant buds of grape 'Kyoho' single-infected with Grapevine fleck virus (GFkV), which is phloem-limited and transmitted by graft inoculation. We produced GFkV-free shoots without thermo- or chemotherapy using meristem-tip explants approximately 0.3 mm (73 explants) and 0.8 mm long (five explants) including shoot apical meristem, 2-5 leaf primordia, and 1-4 uncommitted primordia from dormant buds of the infected woody cuttings (stored at $4^{\circ}C$). Explants were cultured on Murashige and Skoog (MS) medium supplemented with 3% sucrose, 3.0 mg/L benzyladenine (BA) and 0.1 mg/L indole-3-butyric acid (IBA). After 16 weeks of culture, shoot (10-mm long) regeneration frequency achieved from 0.3-mm explants was 4.1% and that obtained from 0.8-mm explants was 40.0%. Virus-free efficiency (expressed as the percentage of RT-PCR negative shoots regenerated) from 0.3- and 0.8-mm explants was 100% and 50%, respectively. Following in vitro multiplication, RT-PCR assays revealed identical results to assays of the first regenerated shoots. Our new methodological approach could be applied for eliminating other viruses in grapevines, as well as for producing virus-free plants in many other deciduous tree species, including fruit trees.