• The Korean Journal of Ecology
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• v.27 no.1
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• pp.49-55
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• 2004

To verify growth properties of Carex kobomugi, above and belowground parts of C. kobomugi were surveyed on coastal sand dune at Sinduri, Choongnam Province from April, 2001 to June 2003. The results were the same as follows. In coastal sand dune, C. kobomugi followed Elymus mollis from mean high tide line. Density of C. kobomugi was the highest at unstable sand dune and 150 plants/㎡. And rates of flowering plant were 70% at unstable area and 10% at stable area. C.kobomugi is thus plant that adapted to unstable sand dune. Shoot distributed irregularly. The rhizome of C. kobomugi gradually decreased with the soil depth in unstable area, but mainly distributed to 20 ㎝ depth. New rhizome could be generated from the rhizome which was below 150 ㎝ depth or 5 years old. The number and length of new rhizome were related to biomass of previous year's shoot. That was, shoots which were below 0.5 and over 3 g/shoot in dry weight generated 1 and 5 rhizomes, respectively. And the mean lengths of rhizome from small (below 0.5 g) and large (over 3.0 g) shoots were 13.0 ㎝ and 57.6 ㎝, respectively.

• Korean Journal of Ecology and Environment
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• v.49 no.2
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• pp.110-123
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• 2016

In order to obtain scientific information for the conservation of Utricularia japonica Makino, rare plant species, we investigated life history, phenology, growth and distribution in the east coastal region of Korea. Seven habitats of U. japonica were confirmed in this study. Turion of U. japonica rests overwinter at the bottom and ripens at the water surface and then usually germinate and sprout when the water warms in spring. A single shoot grown from turion divided into several shoots. Shoot decomposition started in October and formed a new turion at the end of the decomposed shoot. Flowering period was from early July to late October. Percentage of flowering ramets was significantly low as 6.3%. U. japonica showed the fastest growth rate from April to July, the maximum growth in August and the highest biomass in October. U. japonica tended to be concentrated in larger ramets in the water depth of 50 cm~150 cm in Cheonjin lake. The main factors affecting the growth of U. japonica were water temperature and turbidity. The establishment and growth of U. japonica in Cheonjin lake were determined by responses to water temperature with seasonal change and to light conditions caused by the different plants. These conditions affect the temporal and spatial distribution of U. japonica and population change. The findings of this study would be helpful to provide the basic information needed for the conservation and restoration of U. japonica.

• Journal of Bio-Environment Control
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• v.18 no.2
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• pp.107-111
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• 2009

This experiment was conducted to select optimum hydroponic systems for clean and mass production for shoot and root of Echinacea. E. purpurea and E. angustifolia. were grown at 5 different kinds of hydroponic systems; NFT, modified NFT, DFT, aeroponics, and Ebb & Flow for 150 days. The lowest photosynthetic rate and transpiration rate were in Ebb & Flow system in E. purpurea and E. angustifolia at 120 days after transplanting. The shoot fresh and dry weight were high in aeroponic and DFT system of E. angustifolia and in aeroponic and NFT system of E. purpurea at 150 days after transplanting. The root fresh and dry weight of both species were the highest in aeroponic system and next to modified NFT system. They were increased in NFT and Ebb & Flow system for the root length and in aeroponic system for the number of tillering. The results demonstrate that aeroponic system was the most effective for enhancement shoot and root biomass of Echinacea spp. in hydroponics.

• The Plant Pathology Journal
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• v.31 no.3
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• pp.310-315
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• 2015

Certain bacterial species associate with plant roots in soil. The plant growth-promoting rhizobacteria (PGPR) stimulate plant growth and yield in greenhouse and field. Here, we examined whether application of known bacilli PGPR strains stimulated growth and asexual reproduction in the succulent plant Kalanchoe daigremontiana. Four PGPR strains B. amyloliquefaciens IN937a, B. cereus BS107, B. pumilus INR7, and B. subtilis GB03 were applied to young plantlets by soil-drenching, and plant growth and development was monitored for three months. Aerial growth was significantly stimulated in PGPR-inoculated plants, which was observed as increases in plant height, shoot weight, and stem width. The stimulated growth influenced plant development by increasing the total number of leaves per plant. Treatment with bacilli also increased the total root biomass compared with that of control plants, and led to a 2-fold increase in asexual reproduction and plantlet formation on the leaf. Collectively, our results firstly demonstrate that Bacillus spp. promote vegetative development of K. daigremontiana, and the enhanced growth stimulates asexual reproduction and plantlet formation.

• The Korean Journal of Ecology
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• v.20 no.1
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• pp.43-49
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• 1997

Effects of addition of soil ameliorants on the growth and nutrient absorption of Zea mays, Miscanthus sinensis and Phytolacca americana were investigated. Acid soil from Yeocheon Industrial Complex were used for the control plot (AS). We made two treatment plots, acid soil + lime (AS + L) and acid soil + dolomite (AS+D). Ration of acid soil : soil ameliorants in freatment plot was 50 : 1(V:V). Acid soil and soil ameliorants were mixed thoroughly before use. Shoot length of corn in AS+L and AS+D was considerably increased by 141% and 137%, respectively, compared with that in AS. Pokeweed in AS+L and AS+D also increased by 183% and 152%, respectively, compared with that in AS. However, growth of Miscanthus sinensis showed slight difference between the control and the treatment plots. Biomass of corn and porkweed in the treatment plots were also greater than those in the control plots. During the growth experiment with corn, concentrations of Ca and Mg in soil were increased and A1 decreased with increased soil pH in the treatment plots. Amount of aluminum absorbed by corn in the control plot was greater than that in the treatment plots. In case of Miscanthus sinensis, however, aluminum absorption in the control plot was lower than those in the treatment plots.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.292-297
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• 1995

We developed an in vitro assay method for evaluating plant growth promotion and providing an evidence that the growth promotion is rendered by growth enhancing factors. The amendment of culture filtrates of Trichoderma harzianum T95 and Gliocladium virens G872 and G872B in Murashige and Skoog (MS) agar medium enhanced the cotyledon growth of cucumber in terms of fresh weight and primary leaf area of cucumber cotyledon cuttings, of which the treatment of G. virens G872B was the most effective. The mycelial culture filtrate of G872B was more effective in the growth promotion than its conidial germling filtrate. The addition of 1% sucrose in MS mineral medium with 0.1% culture filtrates of the antagonists (T95 and G872B) was optimum for enhancing the effect of the filtrates on the growth of cotyledon cuttings in vitro. When cucumber seeds treated with G872B, Pseudomonas putida Pf3 or the G872B-Pf3 mixture were planted in a greenhouse, the rate of seed germination, biomass of shoot and root, and yield of cucumber fruits were increased, especially by G872B or the G872B-Pf3 mixture. Correspondingly, cucumber fruit yields in early to middle-cycles of harvest were significantly greater in the plots of G872B than the control and Pf3-treated plots, and the final yield was highest in the plots of the G872B-Pf3 mixture applications.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2001.04a
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• pp.38-41
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• 2001

It has been reported that some plants have the potential to metabolize the 2,4,6-trintroluene (TNT) in contaminated soils, sediments and natural water. In this study, the effects of TNT on germination and early seedling development of Abutilion avicennae was characterized in a germination test. Concentration up to 80 mg/L TNT did not affect germination but root and shoot growth, and fresh biomass decreased as TNT concentration increased. A series of axenic hydroponical batch culture of Abutilion avicennae at various initial TNT concentration was used to determine its transformation kinetics, to identify products formed, and to evaluate phytotoxic effects on the TNT transformation process. At higher initial TNT concentrations, TNT removal rate constant decreased, however, total amount of TNT removed was increased in the culture media. Reductive transformation products of TNT were not detected in the plant culture media but higher concentrations of reduced metabolites were detected in the root and stem extracts of plant material at the 7 days of batch incubation. From these results we concluded that Abutilion avicennae has an intrinsic capacity for taking up and transforming TNT.

• The Korean Journal of Ecology
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• v.21 no.3
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• pp.179-186
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• 1998

Effects of lead(Pb) and calcium(Ca) on growth responses, accumulation of heavy metals and nitrogen fixation activities of Melilotus suaveolens seedlings were quantitatively analyzed during growing period. Pb contents of the root treated with 30, 100 ppm Pb and 100 ppm Pb added 100 ppm Ca were 54.1, 90.9 and 26.1 folds higher than that of the control, respectively, at pH 4.2 in 28th days, and heavy metal content of plant increased with increasing of pH and Pb concentration. The melilot plant was classified as a Pb accumulator by higher accumulation of Pb in shoot than that of root. Pb treatments resulted in inhibiton of height and chlorophyll contet, and Ca treatment increased height and chlorophyll content insignificantly at pH 4.2 in 28 days. The plant biomass reduced 49, 60 and 54% at pH 4.2 and 47,53 and 50% at pH 6.5, respectively, by 30, 100 ppm Pb and 100 ppm Pb added 100 ppm Ca treatment. Specific nitrogen fixation of nodules reduced 68.4% and 46.6% by 100 ppm Pb treatment and 3.7% and 24.9% by 100 ppm Pb added 100 ppm Ca at pH 4.2 and pH 6.5, respectively, so Ca inhibited significantly Pb activity and toxicity in acdic pH. Nodule formation were reduced to 33, 33 and 50% at pH 4.2 and 50, 33 and 38% at pH 6.5 by 30, 100 ppm Pb and 100 ppm Pb added 100 ppm Ca, respectively.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.3
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• pp.67-74
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• 2012

This study was carried out to cultivate evergreen-leaved tree container seedlings having low cost of equipment and easy to control. Two species of V. odoratissimum var. awabuki and Q. glauca planting in respective underground container were showed survival rate above 90%. The Relative growth rates of height was good in non-treatment and root collar diameter was no significant difference between treatments. The T/R ratio was respectively 1.1, 1.0 in container type of F, G. and root's biomass products was more effective than others. A container type of F showed better Quailty index than others with 16.2 values. Q. glauca was higher T/R ratio in root development than shoot and the quality index was high in container type of E, D with 4.0. 3.5, respectively. On the basis of the results obtained in this study, we can concluded that the underground container type was suitable to F in V. odoratissimum var. awabuki and to D or E in Q. glauca.

• Journal of Plant Biotechnology
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• v.7 no.3
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• pp.155-160
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• 2005

Haloxylon recurvum (Locally known as Khar) is drought and salt tolerant plant of Thar Desert. This plant is a major biomass producer and has economic and ecological importance for the region. There is need for study on biology, propagation and genetic improvement for utilization of this plant for reclamation of saline soils. We report here on in vitro propagation of Haloxylon recurvum (Moq.) using nodal explant. Secretion of phenolic compound from explants was a major constraint for establishment of culture. This was checked by thorough washing and quick transfer of explant on fresh culture medium. Juvenile nodal explant with leaves was found suitable for culture establishment. Benzy-ladenine($4.0\;{\mu}M$) incorporated in Murashige and Skoog (MS) medium with additives (50 mg/L ascorbic acid and 25 mg/L each of adenine sulphate, arginine and citric acid) induced multiple shoots from nodal explant. Addition of $1.0\;{\mu}M$ naphthalene acetic acid (NAA) in combination with $4.0\;{\mu}M$ BAP improved the growth of axillary shoots. Further shoot amplification was achieved by repeated subculture of mother explants on fresh medium. Forty percent of the micropropagated shoots rooted on half-strength MS medium with $4.0\;{\mu}M$ indolebutyric acid (IBA) and 100 mg/L activated charcoal, at $28{\pm}2^{\circ}C$ and $60\%$ RH. Sixty percent of these plantlets were hardened in green house.