• Asian-Australasian Journal of Animal Sciences
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• 제13권7호
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• pp.980-985
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• 2000

Influences of Japanese green tea powder (GTP) supplementation to commercial diet on laying performance and egg quality were studied by using 60 laying hens. The experimental diet with or without 0.6% GTP was given ad libitum to the birds during the period from 6 to 71 weeks of age. The birds started egg production from 21 wk of age regardless GTP feeding. Body weight, feed intake, egg weight tended to decrease with GTP supplementation, while egg production rate tended to increase. Haugh unit score was significantly increased with GTP, which accompanied with the increased albumen height. These were observed almost throughout the laying period over 50 wk. Gel proportion in thick albumen was decreased as storage time is prolonged, then higher values of the gel proportion were recorded in the eggs from GTP group. Thick albumen of the eggs from GTP-fed layers had more carbohydrate than that from control layers. All these indicate physical stability of thick albumen in the eggs from GTP group. Thiobarbituric acid content of egg yolk tended to remain lower in the eggs from GTP group during 5-10 days of storage at room temperature. Levels of egg yolk cholesterol and yolk lipid were significantly reduced by GTP feeding. There were no significant differences in eggshell weight, shell thickness and shell strength between the two groups. Thyroid gland and liver from hens slaughtered at 71 wk of age did not differ in weight irrespective of GTP feeding. The present results suggest that GTP could modify components of edible part of egg, leading to the characteristics favourable to consumers such as high durability of thick albumen and less cholesterol in yolk, without altering general performance of the layers throughout this year round experiment.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1638-1642
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• 2006

An experiment was conducted to elucidate the effect of graded levels of vitamin $D_3$ in White Leghorn (WL) layer breeders on egg production, shell quality, hatchability of eggs and juvenile performance of offspring during their late laying period (72-88 wk). White Leghorn breeder females were randomly divided into 5 groups of 50 each and were housed in individual California cages in an open-side housing system. Considering birds in five cages as a replicate, 10 such replicates were randomly allotted to each treatment. A basal diet was formulated containing all the nutrients as recommended for WL layers except vitamin $D_3$, which served as control. Another, four diets were formulated by supplementing graded levels of feed grade crystalline cholecalciferol to the basal diet that contained 300, 600, 1,200 and 2,400 ICU of vitamin $D_3$ per kg. Each diet was offered ad libitum to one of the above five treatment groups. The egg production, egg weight, daily feed consumption and the feed intake per dozen eggs or kg egg mass of the birds fed diet without any supplemental vitamin $D_3$ was comparable with those of supplemental groups. Similarly, the level of vitamin in the diet did not have any effect on any of the above parameters. However, the specific gravity of eggs laid by the birds fed the diet without supplemental vitamin $D_3$ was comparable with either 600 or 2,400 ICU supplemental groups but significantly higher when compared to the 300 and 1,200 ICU groups. The egg -shell breaking strength was significantly lowered in the 600 ICU supplemental groups as compared to the strength of other dietary groups. The Haugh unit, egg shell weight, shell thickness, tibia breaking strength, bone ash and calcium content were not influenced by vitamin $D_3$ concentration in the diet. Serum Ca concentration was influenced by vitamin $D_3$ level in the diet. The serum Ca concentration of birds fed either control or the vitamin supplemented diet up to 1200 ICU/kg diet was comparable. However, increasing the concentration of vitamin $D_3$ to 2,400 ICU/kg diet significantly enhanced the concentration of Ca in the serum, which was significantly higher compared to other dietary groups. The serum concentration of P and protein, however, was not influenced by level of vitamin $D_3$ in the diet. Neither fertility nor hatchability was influenced by vitamin $D_3$ concentration in the diet. Feeding a vitamin $D_3$ deficient diet or supplementation of vitamin to hens did not have any influence on their progeny chicks. It can be concluded that dietary supplementation of vitamin $D_3$ may not be essential for optimum production, shell quality, hatchability, and juvenile performance of WL breeders during 72 to 88 weeks of age.

• 한국패류학회지
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• 제17권2호
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• pp.79-84
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• 2001

동해안 가리비 (Patinopecten yessoensis)의 안정적인 인공종묘생산을 위한 기초 연구로서 수온과 염분에 따른 난 발생, 수온과 먹이생물에 따른 유생의 성장과 생존율을 조사하였다. 수정란은 난경 77.3$\pm$2.7 $\mu\textrm{m}$의 분리침성란으로서 산란 6시간 후에 4세포기로 발달하였으며, 수정 60 시간 후에 각장 117$\pm$3 $\mu\textrm{m}$의 D형 유생으로 발달하였다. 수온 10-2$0^{\circ}C$, 염분 28-34 ppt에서 정상 발생이 가능하였으며, 수온이 높을수록 난 발생 속도가 빨라졌다. D형 유생은 수정 10일 후에 각장 160 $\mu\textrm{m}$ 전후의 각정기 유생으로 발달하였으며, 수정 25일 후에 각장 250 $\mu\textrm{m}$로 성장하여 안점이 관찰되는 성숙유생으로 발달하였다. 유생기 동안 각장 (SL)에 대한 각고 (SH)의 상대 성장식은 SH=1.0425SL - 27.731 ($r^2$= 0.9749)였다. 수온별 유생사육에서는 8$^{\circ}C$와 12$^{\circ}C$에서 낮은 성장률을 보였으며, 2$0^{\circ}C$에서 낮은 생존율을 보였으나 16$^{\circ}C$에서 성장과 생존을 모두 양호하였다. 식물 먹이생물에 따른 성장과 생존율은 Isochrysis galbana, Pavlbava lutheri 및 Chaetoceros calcitrans를 혼합 공급하는 것이 가장 효과적이었다.

• Parasites, Hosts and Diseases
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• 제49권3호
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• pp.323-326
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• 2011

While the presence of pinworm eggs in archaeological samples has been reported by many researchers in the New World, those have been detected very scarcely in the Old World, especially in East Asian countries. In fact, many parasite species were recovered from the archeological remains in Korea, eggs of Enterobius vermicularis had not been found. Recently, a female mummy buried in the 17th century was discovered in the Joseon tomb from Dangjin-gun, Chungcheongnam-do, Korea. After rehydration process for 12 days, investigations were carried on the luminal surface of the colon. From them, 3 eggs of E. vermicularis were recovered. They were elliptical, transparent with a thin egg shell, $50.3{\pm}5.2\;{\mu}m$ (length) and $28.2{\pm}3.9\;{\mu}m$ (width) in size. This is the first discovery of E. vermicularis eggs in East Asia.

• 한국발생생물학회지:발생과생식
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• 제16권3호
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• pp.205-217
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• 2012

The ovarian cycle, the biological minimum size, and artificial spawning frequency by artificial spawning induction of the female hard clam, Meretrix petechialis, were investigated by histological observations and morphometric data. The ovarian cycle of this species can be classified into five successive stages: early active stage, late active stage, ripe stage, partially spawned stage, and spent/inactive stage. The spawning period was from June to September, and the main spawning occurred between July and August when the seawater temperature exceeds over $20^{\circ}C$. The biological minimum size (shell length at 50% of first sexual maturity) in females were 40.39 mm in shell length (considered to be two years of age), and all clams over 50.1 mm in shell length sexually matured. In this study, the mean number of the spawned eggs by spawning induction increased with the increase of size (shell length) classes. In case of artificial spawning induction for the clams > 40.39 mm, the number of spawned eggs from the clams of a sized class was gradually decreased with the increase of the number of the spawning frequencies (the first, second, and third spawning). In the experiments of artificial spawning induction during the spawning season, the interval of each spawning of this species was estimated to be 15-18 days (approximately 17 days).

• 한국패류학회지
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• 제26권1호
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• pp.97-105
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• 2010

제주 항 내에 서식하는 대수리 Thais clavigera를 조직학적 방법을 이용하여 1998년 3월부터 1999년 2월까지 생식소 발달과 생식주기 그리고 imposex 현상에 대해 조사하였다. 조직학적 관찰 결과, 배우자형성은 암,수 모두 10월에 처음 시작 되었고, 성숙한 알들은 주로 5월에서부터 7월 사이에 관찰되었다. 생식소단면적 지수 역시 알이 성숙하는 5월과 7월 사이 급격히 증가하였으며, 8월에 급격히 낮아져, 7월과 8월 사이에 집중적인 산란이 있었던 것으로 사료되었다. 유기주석화합물에 의한 imposex 현상의 진행 정도를 나타내는 RPSI는 월 평균 59.5-173.4%로 다른 지역에 비하여 비교적 높게 나타났으며, 이는 제주 항 내의 유기주석화합물에 의한 것으로 추정되었다.

• 한국패류학회지
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• 제21권2호
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• pp.81-93
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• 2005

Reproductive cycle with the gonadal phases, first sexual maturity, artificial spawning amount by the size and spawning interval of the hard clam, Meretrix lusoria were investigated by histological observations and morphometric data by artificial spawning induction. Meretrix lusoria is dioecious and oviparous. The reproductive cycle of this species can be classified into five successive stages: early active stage (January to March), late active stage (February to May), ripe stage (April to August), partially spawned stage (June to September), and spent/inactive stage (September to February). The spawning period was from June to September, and the main spawning occurred between July and August when the seawater temperature exceeds over $20^{\circ}C$. Percentage of first sexual maturity of female and male clams ranging from 40.0 to 45.0 mm in shell length was over 50%, and all clams over 50.0 mm in shell length sexually matured. Female and male clams ranging from 40.0 to 45.0 mm in shell length are considered to be two years old. Therefore, we assume that the hard clams of both sexes begin reproduction from two years of age. The mean number of the spawned eggs increased with the increase of size (shell length) classes. In case of artificial spawning induction, the number of spawned eggs from the clams of a sized class was gradually decreased with the increase of the number of the spawning frequencies (the first, second, and third spawnings). In the experiments of artificial spawning induction during the spawning season, the interval of each spawning was estimated to be 15-18 days (average 17 days).

• Asian-Australasian Journal of Animal Sciences
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• 제25권11호
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• pp.1611-1616
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• 2012

The aim of the study was to evaluate the effect of sodium nitrate consumption on egg quality and quantity, and some blood parameters of native breeder hens of West Azerbaijan province. One hundred native hens were used from wk 25 to 32 of age. These birds were divided into two groups. One group was fed the control diet (CD) but the other fed the same diet supplemented with 4.2 g/kg sodium nitrate (ND). After 2 wks of adaptation, eggs were collected daily and egg mass and egg production were measured weekly for five weeks. To assess the egg quality parameters, two eggs from each replicate pen were collected for three consecutive days each week. At the end of experimental period (wk 32 of age), blood samples of 5 birds per replicate were collected from the wing vein into anticoagulant tubes. Dietary sodium nitrate didn't affect the egg production, shell stiffness, shell thickness and Haugh unit (p>0.05) but it decreased the both egg production and egg mass during the last three weeks (wks 30, 31 and 32) (p<0.05). Furthermore, a treatment effect was observed for yolk colour (p<0.05). Both the egg production and egg mass were increased over time (p<0.05). No significant treatment${\times}$time interaction was observed for egg weight, egg production and egg mass (p>0.05). No effect of time or treatment${\times}$time were observed for shell stiffness (p>0.05). Over time, shell thickness was decreased while Haugh unit increased (p<0.05). None of the blood TP and TG or the activity of ALT, AST and LDH enzymes were affected by dietary consumption of sodium nitrate at wk 32 of age (p>0.05). Sodium nitrite decreased both the TAC and TC at wk 32 of age (p<0.001). It was concluded that the lower body antioxidant capacity of nitrate fed birds resulted in the lower performance (egg weight, egg production and egg mass).

• Ocean and Polar Research
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• 제38권1호
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• pp.21-33
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• 2016

Gametogenesis of the comb pen shell, Atrina pectinata (Linnaeus, 1767) (Bivalvia: Pinnidae) on the southern coast of Ulleungdo Island, Korea was assessed monthly (November 2013 to October 2014) using histology. Gametogenesis commenced in January when the surface water temperature was $12.6^{\circ}C$ and pen shells evidenced an early development phase with small oogonia from January to April, although few females exhibited ripe eggs in their follicular epithelium. In April, the oocyte diameter increased rapidly, and fully mature eggs appeared in May. First spawning males and females were observed in June as the surface water temperature reached $19.3^{\circ}C$ and July ($23.2^{\circ}C$) respectively. The spawning activity continued until the end of September. Histology indicated that the spawning peak of the females in Ulleungdo Island was July to August. During October to January, most of the pen shells were in spent and resting stages. Our data suggested that A. pectinata is a summer spawner, and their annual gametogenesis is closely associated with the seasonal variation in the surface water temperature. The present study is the first provided fundamental information on the life history of A. pectinata in Ulleungdo Island, and this can be put to good use in the management of this pen shell in the study area.

• 한국유기농업학회지
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• 제16권2호
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• pp.205-218
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• 2008

본 연구는 산란계에 대한 유기사료 첨가제로서 소나무 껍질 추출물, 피타민의 첨가효과를 조사하기 위하여 산란계(Hyline brown) 150마리를 이용하여 산란성적과 계란품질 평가를 실시하였다. 대조구, 피타민 0.1%, 피타민 0.2%로 구분된 3개의 처리구를 완전 임의배치하여 6주 동안 사육하였다. 산란성적은 피타민 0.1% 첨가구와 대조구가 서로 비슷하였으나 피타민 0.2% 첨가구는 가장 낮았다(p<0.05). 호우유니트는 피타민 0.1% 및 0.2% 첨가구가 대조구에 비해서 높게 나타났으며(p<0.05), 난각두께 및 파란강도는 피타민 0.1% 첨가구와 대조구 사이에 있어서 유의차가 없었다. 계란의 콜레스테롤은 피타민 0.1% 첨가구가 가장 낮았으며 통계적 유의차가 있었다(p<0.05). 계란의 포화지방산 조성은 피타민 0.2% 첨가구가 가장 낮았고 불포화지방산은 피타민 0.2% 첨가구가 가장 높았다(p<0.05). 저장 일수에 따른 계란의 호우유니트, 난황지수 및 난백지수는 피타민 0.1% 및 피타민 0.2% 첨가구가 대조구에 비해서 유의적으로 높았다(p<0.05). 결론적으로 소나무 껍질추출물, 피타민을 산란계 사료 내 유기사료 첨가제로서 0.1% 수준으로 첨가해주면 산란능력 및 계란의 품질을 일정하게 유지하면서 계란 저장성을 높일 수 있을 것으로 나타났다.