• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 춘계학술대회
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• pp.1678-1681
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• 2004

The light-transmission technique has been applied to a slit rheometer for measuring red blood cell aggregation as well as blood viscosity over a range of shear rates. For measurement of blood viscosity and aggregation, instantaneous pressure and transmit-light intensity are measured with time. Using a precision pressure measurement, one can determine the shear stress and shear rate. In addition, a transmitted light through a blood sample indicates degree of RBC aggregation. With abruptly flowing with high shear rate, RBCs rapidly disaggregate and the intensity of the transmitted light becomes low. When continuously flowing with decreasing shear rate, RBCs tend to re-aggregate and the corresponding transmit-intensity gradually increases with time. The light intensity as a degree of RBC aggregation is plotted against shear rate and compared with blood viscosity. The advantages of this design are dual measurement at a time, simplicity, i.e., ease of operation and no moving parts, low cost, short operating time, and the disposable kit which is contacted with blood sample.

• Biotechnology and Bioprocess Engineering:BBE
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• 제1권1호
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• pp.32-35
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• 1996

Amoving aeration-membrane (MAM) bioreactor was employed for the production of 2$\mu$g/mL of tissue type Plasminogen Activator (tPA)in serum free medium from normal human fibroblast cells. This system could maintain high cell density for long periods of steady state conditions in perfusion cultivation. Under normal operating condition, shear stress was as low as 0.65 dynes/$\textrm{cm}^2$ at the agitation speed of 80 rpm. Even though cell density gradually decreased with increasing agitation speed, tPA production increased linearly with increasing shear stress within a moderate range. This culture system allowed production of 2$\mu$g tPA/mL while maintaining a high cell denisty of 1.0$\times$107 viable cell/mL.

• International Journal of Aeronautical and Space Sciences
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• 제4권1호
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• pp.9-18
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• 2003

In this work, a closed-form analysis is performed for the structural response of coupled composite blades with multi-cell sections. The analytical model includes the effects of shell wall thickness, transverse shear, torsion warping and constrained warping. The mixed beam approach based on Reissner's semi-complementary energy functional is used to derive the beam force-displacement relations. The theory is validated against experimental test data and other analytical results for coupled composite beams and blades with single-cell box-sections and two-cell airfoils. Correlation of the present method with experimental results and detailed finite element results is found to be very good.

• KSBB Journal
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• 제13권6호
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• pp.700-705
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• 1998

Studies were made to investigate effects of the scale-up of stirred tank bioreactors on cell growth and alkaloids production for suspension cultures of Eschscholtzia californica. In the 1.5 L STR, cell lysis was observed at 110 rpm or higher agitation speed. The agitation speed of 30 L STR was 43.7 rpm to maintain the same shear stress developed in 1.5 L STR of 100 rpm. As a result of scale-up from 1.5 L to 30 L STR, the specific growth rate was decreased from 0.12 to 0.07 day-1. The alkaloids productivity was also decreased from 0.24 to 0.14 mg/L-day. Changes of mixing performance and oxygen transfer were studied to explain the decrease of cell growth and alkaloids production. Decreased oxygen transfer rate coefficient(KLa) and increased mixing time by the scale-up was observed at various aeration rates.

• Imaging Science in Dentistry
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• 제48권3호
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• pp.161-165
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• 2018

Purpose: To evaluate the usefulness of shear wave elastography in the diagnosis of oral and maxillofacial diseases. Materials and Methods: Ten patients with oral and maxillofacial diseases and 28 volunteers drawn from our student doctors were examined by shear wave elastography with a 14-MHz linear transducer using an Aplio 300 apparatus (Canon Medical Systems, Otawara, Japan). A statistical analysis of the shear elastic modulus(kPa) of healthy tissue (the sublingual gland, submandibular gland, anterior belly of the digastric muscle, and geniohyoid muscle) in the 28 volunteers was performed using 1-way repeated measures analysis of variance with the Tukey honest significant difference test. The maximum shear elastic modulus(kPa) of 8 patients with squamous cell carcinoma (SCC) and 2 patients with benign lesions was evaluated with the Mann-Whitney U test. The analysis used a 5% significance level. Results: The mean shear elastic modulus of the sublingual gland ($9.4{\pm}3.7kPa$) was lower than that of the geniohyoid muscle ($19.2{\pm}9.2kPa$, P=.000) and the anterior belly of the digastric muscle ($15.3{\pm}6.1kPa$, P=.004). The maximum shear elastic modulus of the SCCs($109.6{\pm}14.4kPa$) was higher than that of the benign lesions($46.4{\pm}26.8kPa$, P=.044). Conclusion: Our results demonstrated the usefulness of shear wave elastography in the diagnosis of oral and maxillofacial diseases. Shear wave elastography has the potential to be an effective technique for the objective and quantitative diagnosis of oral and maxillofacial diseases.

• 대한기계학회논문집A
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• 제20권9호
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• pp.2782-2791
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• 1996

The effective longitudinal shear modulus(LSM) of continuous composites is studied theoretically and numerically using 3-phase unit cell model. Circular, hexagonal and rectangular shapes of reinforced fiber are considered to predict the shear modulus as a function of elastic modulus of each phase and volume fraction of interphase and reinforced fiber. It is found that rectangular fiber shape in low fiber volume fraction($v_f$<30%) and circular fiber shape in high volume fraction($v_f$>40%) shows the higher longitudinal shear modulus. Also the obtained values of LSM for rectangular array and by numerical analysis are higher than those of hexagonal array and by theoretical analysis respectively. The reinforcing effects of interphase are more significant in cases of higher fiber volume fraction and circular fiber shape. Not only the spatial distribution and shape of reinforcing fiber but also the volume of interphase have a pronounced effects on the overall LSM. It is also found that the tangent moduous of 2-and 3-phase polymer matrix composites is insensitive to the shape and distribution of reinforcing fibers.

• Journal of Mechanical Science and Technology
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• 제14권8호
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• pp.861-866
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• 2000

Flow induced shape change is important for spatial interpretation of vascular response and for understanding of mechanotransduction in a single cell. We investigated the possible shapes of endothelial cell (EC) in a mathematical model and compared these with experimental results. The linearized analytic solution from the sinusoidal wavy wall and Stokes flow was applied with the constraint of EC volume. The three dimensional structure of the human umbilical vein endothelial cell was visualized in static culture or after various durations of shear stress (20 $dyne/cm^2$ for 5, 10, 20, 40, 60, 120min). The shape ratio (width: length: height) of model agreed with that of the experimental result, which represented the drag force minimizing shape of stream-lining. EC would be streamlined in order to accommodate to the shear flow environmented by active reconstruction of cytoskeletons and membranes through a drag force the sensing mechanism.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1487-1491
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• 2008

Detailed knowledge on the motion of blood cells flowing in micro-channels under simple shear flow and the influence of blood flow is essential to provide a better understanding on the blood rheological properties and blood cell aggregation. The microscopic behavior of red blood cell (RBCs) is numerically investigated using a fluid-structure interaction (FSI) method based on the Arbitrary-Lagrangian-Eulerian (ALE) approach and the dynamic mesh method (smoothing and remeshing) in FLUENT (ANSYS Inc., USA). The employed FSI method could be applied to the motions and deformations of a single blood cell and multiple blood cells, and the primary thrombogenesis caused by platelet aggregation. It is expected that, combined with a sophisticated large-scale computational technique, the simulation method will be useful for understanding the overall properties of blood flow from blood cellular level (microscopic) to the resulting rheological properties of blood as a mass (macroscopic).

• 한국해안·해양공학회논문집
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• 제29권2호
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• pp.83-91
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• 2017

이상기후에 따른 대형 태풍 내습 시 고파랑 대응을 위한 항만구조물의 안정성 향상 기술이 대두되면서 신형식 인터로킹 케이슨의 설계 및 시공기술에 관한 연구가 활발히 진행되고 있다. 기존 인터로킹 방식에서 응력집중현상에 대한 대안으로 채움재를 이용한 오픈 셀 케이슨은 채움재 마찰에 의한 저면(일부) 및 인접케이슨과의 결속력을 증대시켜 평활화효과를 가져온다. 이러한 오픈 셀 케이슨은 시계열 수평파력에 대한 전단 횡격벽의 응력집중을 파악함으로써 부재 설계 시 안정성을 확보하기 위해 오픈 셀 케이슨의 내 외부벽에 의한 사석 전단저항을 살펴볼 필요가 있다. 이에 본 연구에서는 포항북방파제에 설치되었던 케이슨의 제원 및 설계조건을 바탕으로 해석대상을 선정하였으며, 채움재는 사석으로 가정하여 인터셀에 작용하는 전단력을 산정하고 내 외부벽 제원에 따른 전단거동을 수치해석을 통해 살펴보았다. 내 외부벽 배치 개수 및 연결부 사석두께 대비 외부벽의 길이를 변수로 저항력을 분석하였다. 제원에 따라 내 외부벽이 분담하는 비율을 분석한 결과, 외해 측에 있는 외부벽이 전체 전단력의 30% 이상을 저항하며 하중을 가장 많이 분담하였고, 내부벽이 거의 유사하게 60~70% 하중을 분담하였다.

• Korea-Australia Rheology Journal
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• 제16권3호
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• pp.129-134
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• 2004

The aggregation characteristics of red blood cells (RBCs) were measured using a newly developed light-transmission slit rheometer. Conventional methods of RBC disaggregation such as the rotational Couette system were replaced with a pressure-driven slit flow system with a vibrational mechanism. Using a vibration generator, one can disaggregate the RBC aggregates stored in the slit. While shear stress decreases exponentially, instantaneous pressure and the transmitted light intensity were measured over time. Applying an abrupt shearing flow after disaggregation caused a rapid elongation of the RBCs followed by loss of elongation with the decreasing shear stress. While the shear stress is further decreasing, the RBCs start to re-aggregate and the corresponding transmitted intensity increases with time, from which the aggregation indices can be obtained using a curve-fitting program.