• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.341-348
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• 2011

Sloan-Kettering virus gene product of a cellular protooncogene c-Ski is an unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. The aim of the present study was to locate Ski protein in rat ovaries in order to predict the possible involvement of Ski in follicular development and atresia. First, expression of c-Ski mRNA in the ovaries of adult female rats was confirmed by RT-PCR. Then, ovaries obtained on the day of estrus were subjected to immunohistochemical analysis for Ski and proliferating cell nuclear antigen (PCNA) in combination with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Ski was expressed in granulosa cells that were positive for TUNEL, but negative for PCNA, regardless of the shape and size of follicles. Expression of Ski in TUNEL-positive granulosa cells, but not in PCNA-positive granulosa cells, was also verified in immature hypophysectomized rats having a single generation of developing and atretic follicles by treatment with equine chorionic gonadotropin (eCG). These results indicate that Ski is profoundly expressed in the granulosa cells of atretic follicles, but not in growing follicles, and suggest that Ski plays a role in apoptosis of granulosa cells during follicular atresia.

• Korean Journal of Human Ecology
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• v.8 no.2
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• pp.327-337
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• 1999

Primitive men's primordial formative activity derives from incarnation contained in intensive vitality and strong creativity mind. The trend of modern fashion requiring the boundless imagination can be called that it, just, itself, has something in common with basic spiritual activity that primitive men had. What is concerning how modern fashion designers have developed formative language through enchanting primitive art is as follows. 1. The nature's mysterious power appears to be transformed into personified image. The incarnation for acquiring beauty, power, prestige of genius appears in the form of personification, making use of mask in modern fashion. 2. As for primitive men, the whole universe has a potentiality as a symbol. The symbolism of primitive art based on religion and myth appears in modern art as the form of presenting oppositional objects together in one space. 3. Primitive art is the purest form and the most unpolluted. This shows the natural quality being assimilated into nature, which is expressed in modern fashion as intensity, free-spirit, simplicity, etc. 4. The primitive men's anxiety to the outside world appears as impulse. The geometrical form of primitive art occurred in the shape of impulse appears in expressional form of modern fashion. 5. The real existence in primitive art inducing real materials and objects, in themselves, into formative world appears in modern art in the way of expression such as repetition, enlargement, exact reproduction.

• Reproductive and Developmental Biology
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• v.38 no.2
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• pp.79-84
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• 2014

MicroRNAs (miRNAs) are approximately 22 nucleotides of small noncoding RNAs that control gene expression at the posttranscriptional level through translational inhibition and destabilization of their target mRNAs. The miRNAs are phylogenetically conserved and have been shown to be instrumental in a wide variety of key biological processes including cell cycle regulation, apoptosis, metabolism, imprinting, and differentiation. Recently, a paper has shown that expression of the miRNA-302/367 cluster expressed abundantly in mouse and human embryonic stem cells (ESCs) can directly reprogram mouse and human somatic cells to induced pluripotent stem cells (iPSCs) efficiently in the absence of any of the four factors, Oct4, Sox2, c-Myc, and Klf4. To apply this efficient method to porcine, we analyzed porcine genomic sequence containing predicted porcine miRNA-302/367 cluster through ENSEMBL database, generated a non-replicative episomal vector system including miRNA-302/367 cluster originated from porcine embryonic fibroblasts (PEF), and tried to make porcine iPSCs by transfection of the miRNA-302/367 cluster. Colonies expressing EGFP and forming compact shape were found, but they were not established as iPSC lines. Our data in this study show that pig miRNA-302/367 cluster could not satisfy requirement of PEF reprogramming conditions for pluripotency. To make pig iPSC lines by miRNA, further studies on the role of miRNAs in pluripotency and new trials of transfection with conventional reprogramming factors are needed.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.11a
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• pp.231-235
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• 2009

The mainly objectives of this study was a combustion dynamics and instability characteristics in a model dump type combustor which is scale down of GE 7FA+e DLN 2.6 gas turbine combustor with running at Seo-Inchon combined cycle power plant. Model gas turbine injector has 2-stage swirl vane and it's reduced 1/3 size of the original one. The shape of plenum and combustor were designed for similar acoustic characteristics. As the result, this research have been shows the peak frequency of model combustor was changed quarter-wave mode to Helmholtz resonator mode in plenum and longitudinal mode in dump combustor at unstable flame conditions caused by the different of combustor temperature and fuel-air mixture distributions.

• Proceedings of the IEEK Conference
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• 2001.10a
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• pp.76-79
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• 2001

In Japan in of today, there is no aspect in which the quantity of waste glass bottle discharged from ordinary homes decreases. Moreover, the kind of shape and color of the waste glass bottles are increasing little by little. In the recycling of the glass bottle, it becomes a problem that many kind of the color is abounding. It is possible to use the transparent glass bottle as raw material of the glassware. Since the reproduction of the color is difficult, the colored glass bottle is not possible to use as a raw material. Therefore, the processing of these glass bottles entirely depends on the reclamation. In Japan, the security of the new reclaimed land is very difficult for the reason of the environmental damage. Moreover, the life expectancy in many reclaimed lands is anticipated with within two years. Therefore, the development of the processing method of glass bottle other than reclamation becomes a rapid problem. At present it is examined that it is used of an aggregate of the colored pavement, as one of the application methods of colored glass bottle. The particle size of produced glass aggregate is also 5mm or less, because the thickness is 5mm or less on almost glass bottles. And almost glass particles have the sharp tip. The application as the aggregate is limited for this sharpness of the particle. This study was carried out for the purpose of the establishment of sharpness measuring method of the glass aggregate particle. It is possible that the injury degree to human in the handling is known, if showing the sharpness of the aggregate particle at the objective numerical value is possible. And the application of the glass aggregate is spread. In this research, the balloon fracture method was used for the sharpness measurement of the glass particle. This method is based on the completely new idea, and it is possible to express completely the possibility of injury to the human. It is reported with the detailed result in full paper, because this study is continuous in order to get the JIS which is industrial standard of Japan.

• Smart Structures and Systems
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• v.26 no.5
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• pp.657-666
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• 2020

A rotational pulse-echo ultrasonic propagation imager that can inspect cylindrical specimens for material nondestructive evaluations is proposed herein. In this system, a laser-generated ultrasonic bulk wave is used for inspection, which enables a clear visualization of subsurface defects with a precise reproduction of the damage shape and size. The ultrasonic waves are generated by a Q-switched laser that impinges on the outer surface of the specimen walls. The generated waves travel through the walls and their echo is detected by a Laser Doppler Vibrometer (LDV) at the same point. To obtain the optimal Signal-to-Noise Ratio (SNR) of the measured signal, the LDV requires the sensed surface to be at a right angle to the laser beam and at a predefined constant standoff distance from the laser head. For flat specimens, these constraints can be easily satisfied by performing a raster scan using a dual-axis linear stage. However, this arrangement cannot be used for cylindrical specimens owing to their curved nature. To inspect the cylindrical specimens, a circular scan technology is newly proposed for pulse-echo laser ultrasound. A rotational stage is coupled with a single-axis linear stage to inspect the desired area of the specimen. This system arrangement ensures that the standoff distance and beam incidence angle are maintained while the cylindrical specimen is being inspected. This enables the inspection of a curved specimen while maintaining the optimal SNR. The measurement result is displayed in parallel with the on-going inspection. The inspection data used in scanning are mapped from rotational coordinates to linear coordinates for visualization and post-processing of results. A graphical user interface software is implemented in C++ using a QT framework and controls all the individual blocks of the system and implements the necessary image processing, scan calculations, data acquisition, signal processing and result visualization.

• Journal of The Korean Association of Information Education
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• v.3 no.1
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• pp.134-142
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• 1999

The demand for recognizing the color as well as the object shape is increasing to use the detailed information, because-the expense of color input/output devices become cheap. The research on the color correction should be researched for the exact color presentation and color reproduction of color input/output systems. In this paper, we researched on the color correction of color scanner. The characterization of color scanner is a two step process of gray-balancing and color transformation. The decoupling of the gray-balancing from the color transformation enables the portability of the scanner characterization. We used the least square methods for the line fitting and the Neural Network for the storage space and computation speed. The output of Neural Network is similar to the target value in three-dimensional tristimulus space. The proposed color correction method can be used for all scanners of a manufacturer's model because of the portability.

• Development and Reproduction
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• v.20 no.4
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• pp.283-288
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• 2016

The purpose of the present study was to investigate the morphological characteristics of the developing vallate papillae (VP) of Korean native goats using scanning electron microscopy. In prenatal development of the VP, primordia of the VP were observed and the moat was shallowly spread in 60-day-old fetuses. The moat of the vallate papillae was shallowly spread and still undifferentiated in 90-day-old fetuses. The trench wall of the moat of the VP was well developed in 120-day- old fetuses. In neonates, the moat of the VP was more widely and deeply engraved and VP were developed as completely as those of adults. In postnatal development, VP were observed to have continually increased in size with slight morphological changes until 90-days after birth. Taste pores of the VP were shaped like flower leaves in 120-days after birth. The microridges and microplicaes were well developed on the epithelial surface of the VP in goats ranging from 120-day-old fetuses to 120-day-old postnatal animals. These results suppose that the sensing ability for gestation of VP was already well developed by the time of its birth and VP were differentiated into a variety of different shape and size during development.

• Journal of Life Science
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• v.18 no.6
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• pp.759-763
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• 2008

Halophila ovalis (R. Br.) Hook. fil. was first collected from the Ando, Yeosu, Korea in 2007. H. ovalis is Widely distributed from sub-tropical to even tropical areas and produces the seeds using bisexual reproduction. Its leaf shape was oblong to ovate. Its leaf blades were rigid in texture, with a strong support to the leaf. Erect shoots arose at irregular intervals along the rhizome. The distance between the intramarginal vein and leaves margin was small. Nucleotides in ITS 1 and ITS 2 regions between the Korean and Japanese H. ovalis were found to be 100% similar, whereas Korean H. ovalis was found to have four nucleotides in the positions of 202 bp to 206 bp for 5.8S. In the analysis of the phylogenetic relationship using NJ method, Korean H. ovalis had a monophyletic genetic tree with Japanese H. ovalis, but no phylogenetic relationship with types from the Philippines, Australia, Malaysia, and Vietnam. The first occurrence of H. ovalisin Korea was associated with a strong migration of gene flow from Japan and high water temperature caused by the variations in climate.

• Development and Reproduction
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• v.19 no.4
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• pp.235-242
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• 2015

Black soybean teata is helpful to preventing obesity through enhancing energy expenditure and suppressing accumulation in mesenteric adipose tissue. The ethanol testa-extract of Cheongja #3 black soybean (ETCBS) is also have similar effects on obesity. So far, it is not clear whether the ethanol testa extract of black soybean can have effect on the characters of subcutaneous adipose stem cells such as proliferation, activity, and adipogenicity. The doubling time was different between subcutaneous adipose-derived stem (ADS) and visceral ADS cells. By the in vitro culture and passage, the doubling time was increased both of them. The shape was not different between groups and their passages were not cause the change of shapes. In the case of visceral ADS cells, the doubling time was 62.3 h or 40.3 h in control or high fat diet administrated mice, respectively, but not modified in subcutaneous ADS cells. ETCBS administration caused of increased the doubling time from 62.3 h to 84.2 h. ETCBS had suppressive effects on the cellular activity of subcutaneous ADS cells. The intensity of Oil Red O staining was very faint in 100 and $200{\mu}g/mL$ ETCBS treated groups. The amounts of accumulated triglyceride were also significantly low in 100 and $200{\mu}g/mL$ treated groups. From these results we know that the doubling times and the effects of ETCBS are different by the anatomical origin of ADS cells. It also suggested that ETCBS may suppress the differentiation of subcutaneous ADS cells into the precursors and maturing of adipocytes.