• 한국식품위생안전성학회지
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• 제14권1호
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• pp.45-54
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• 1999

The purpose of this study was to examine the increased bone loss caused by ovariectomy through monitoring the concentrations of the collagen and the pyridinoline crosslinks of collagen. The ovariectomized rats treated for 8 weeks, were divided at random into two or three groups of 10. Ovariectomies were carried out from the saline-treated group (Ovx), the estrogentreated group (Ovx+ES) and chondroitin sulfate-treated group (Ovx+CS). Sham operations were performed on the sham-operated group (Sham). Ovx+ES and Ovx+CS groups showed the remarkably increased collagen and pyridinoline amount in the bone and cartilage compared to Ovx group. And as the result of the measurement of SOD, Catalase and GPx which are antioxidant enzyme, SOD and Catalase activities in Ovx group were much higher than in Sham group. But they were significantly decreased in Ovx+CS group. Based on these results, it is supposed that estrogen and condroitin sulfate can enhance collagen synthesis and affect the pyridinoline formation in collagen fibrils through stimulating lysyl oxidase activity. And it is also thought that chondroitin sulfate can inhibit aging by reducing antioxidant enzyme.

• 국제물리치료학회지
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• 제8권1호
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• pp.1071-1076
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• 2017

The purpose of this study was to determine if a gentle form of manual lumbar traction could reduce painful lumbar motions associated with lumbar disc degeneration (LDD). This clinical trial incorporated 134 participants with painful active lumbar motion. Participants were randomly assigned to an experimental treatment or sham group. 67 participants received sidelying manual lumbar traction while the other 67 participants received a sham treatment. Pre and post treatment NPRS values for the painful active lumbar motion were recorded for each group. There was a statistically significant improvement (P=0.00) for decreased pain intensity during active lumbar motion in the experimental group as compared to the sham treatment group. The average percent decrease in numeric pain rating scale (NPRS) values was 52.1% for the experimental treatment group and 8.1% for the sham group. The results of the study suggest that side-lying manual lumbar traction can improve painful lumbar motion in patients with LDD.

• 한국식품영양과학회지
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• 제33권2호
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• pp.324-330
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• 2004

난소 절제시술 및 폐경으로 인한 여성 호르몬인 estrogen의 감소는 지속되는 골 손실 가속화 등으로 인한 골 질환의 문제점을 가지고 있다. 그러므로 본 연구에서 흰쥐의 난소를 절제하여 인위적인 폐경을 유도하여 골손실의 정도를 결합조직 중의 collagen 함량 및 collagen성숙가교인 pyridinoline함량을 측정하여 그 효과를 검토하였다 난소 절제 후 결합조직 중의 collagen함량은 연골과 피부에서 난소를 절제하지 않은 Sham군에 비하여 낮았으며, 김 추출물 투여군에서 유의적이지는 않으나 높은 경향을 나타내었다. 골 중 collagen성숙가교인 pyrldinoline 함량은 난소절제에 의해 감소하였으나 골에서는 김 추출물 투여에 의해 난소를 절제하지 않은 Sham군과 비슷한 수준으로 증가하였고, 연골에서는 김 추출물 투여에 의해 Sham군과 비슷한 수준으로 감소하는 경향을 나타내어 pyridinoline 생성에 유익한 효과를 나타내었다. 김 추출물이 collagen합성 및 성숙가교인 pyridinoline 생성을 회복시킨 본 실험의 결과로 보아 폐경기여성에게 김 추출물의 섭취는 폐경으로 인한 골손실을 감소시켜 줄 것으로 사료되어지며, 이러한 작용을 나타낸 김의 성분 및 그 기전에 대해서는 앞으로 더 많은 연구가 필요할것으로 생각된다.

• Journal of Nutrition and Health
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• 제38권1호
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• pp.40-47
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• 2005

This study was performed to determine the effect of three different concentrations of soy-isoflavones on calcium and phosphorus balance in either sham-operated or ovariectomized female rats. Seventy-two 16-week old Sprague-Dawley rats underwent sham operation or bilateral ovariectomy. They were provided diets containing different levels of soy iso-flavones for 6 weeks: 50 ppm (Jow isoflavone intake; LI) , 250 ppm (medium isoflavone intake; MI) and 500 ppm (high isoflavone intake; HI). The subsequent fecal and urinary excretions of calcium and phosphorus were then measured. In the sham-operated rats, body weight gains and food efficiency ratio of the MI and HI groups were significantly lower than the control group while food intake was not different. However, there was no significant difference in the ovariectomized rats. The fecal excretion of calcium was significantly lower in the LI, MI and HI groups than the control group in sham operated rats, and significantly lower in the HI group than the control group in ovariectomized rats. Also, apparent ab-sorption rate of calcium and phosphorus did not show any significant difference among groups. Urinary excretion of calcium and phosphorus was significantly lower in the HI group than the LI group in the sham-operated rats. Urinary excretion of calcium was significantly higher in the control ovariectomized rats than in the control sham-operated rats. Retention of calcium and phosphorus did not show any significant difference in both groups. From the above result, we see that isoflavone intake increases calcium retention through an increase in calcium absorption and also suppresses the increase of calcium excretion in urine in ovariectomy. Therefore, it is suggested that isoflavone intake is recommended for menopausal women who experience sharp bone loss due to the decrease in estrogen honnone.

• Journal of Acupuncture Research
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• 제23권6호
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• pp.133-143
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• 2006

Objectives : To Investigate Severity Measurement and the Correlation between Acupoints on the Face and the Upper limb in Bell's Palsy Patients by Using of DITI. Methods : We recreated the persons in the Wonkwang oriental hospital, KwangJu and the public health center, Hwasoon from April 2005 to October 2005. the 60 persons randomized allocated were not showed the acupuncture and treated in the acupuncture points used for treatment of the shoulder pain. the 30 persons were treated with real acupuncture and the others were treated with Kiln Sham Acupuncture. Conclusion : This report suggest that we must except Kyon-lyo(TE14), kyon-u(LI15), and hu-gye(SI03) from the shoulder pain study with Kim Sham Acupuncture because the persons significantly distinguished Real acupuncture from Sham acupuncture at the Kyon-lyo(TE14), kyon-u(LI15) and hu-gye(SI03) and so We cannot blind a person.

• 대한한방소아과학회지
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• 제20권2호
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• pp.19-34
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• 2006

Objective : The purpose of this study is to find out the effect of Jowonsangamibang(JWS) on the growth of rats. Methods : First of all, we divided male Sprague-Dawley rats into 4 groups(J1, J2, J3 and sham group). Then J1, J2 and J3 groups were administered with JWS water extracts(JWSE) once a day at a dosage 250, 500, 1,000mg/kg respectively for 3 weeks. The sham group was administered with normal saline in the same manner. And we measured body weight, amount of body weight increased, length of femur, serum GH, serum IGF-I, serum TSH and serum testosterone after each week. Results : There were no significant changes of body weight, the level of GH, TSH, and testosterone in J1, J2 and J3 groups. The change of body weight gained was increased significantly in the J3 group compared with the sham group after 1, 2 and 3 weeks. The length of the femur was increased significantly in the J2 group after 1 week and in the J3 group after 3 weeks compared with the sham group. The level of IGF-I in the serum was increased significantly in J1, J3 after 1 week compared with the sham group. Conclusion : According to the results above, JWS increased body weight, length of femur, serum GH, serum IGF-1 compared with the sham group. This study shows that JWS has an effect on promoting the growth of rats, thus it's expected to treat of growth delay in children.

• 한국수산과학회지
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• 제31권6호
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• pp.785-790
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• 1998

인체용 마취제인 염산리도카인을 사용하여 버들개, Rhynchocypris steindachneli의 운송실험을 실시하였다. 대조군. sham 대조군 및 염산리도카인 농도 2.5ppm군, 5ppm군, 10ppm군, 20ppm군을 대상으로 실험후 30분, 60분, 90분, 120분, 240분 및 360분에 용존산소, ventilation rate, ammonia성 질소 및 pH를 측정하였다. 실험후 시간 경과에 따라 고농도 염산리도카인군, sham 대조군, 대조군 순으로 용존산소량이 높았으며 ventilation rate도 용존산소량의 결과와 상응하였다. 염산리도카인군은 물질대사가 저하하였기에 물질대사물인 ammonia 배출이 대조군에 비해 낮았고, 염산리도카인군에서는 농도의존적으로 ammonia 농도가 낮아졌다. 실험후 시간경과에 따른 sham 대조군과 염산리도카인군의 pH 감소는 대조군의 pH 감소에 비해 그 감소 정도가 약하게 나타났다. 본 연구 결과 염산리도카인은 버들개의 운송실험시 진정제로 효과적이었다.

• 동의생리병리학회지
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• 제28권1호
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• pp.107-112
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• 2014

This study aimed to prove the effects of acupuncture treatment on acupoint Joksamni(ST36) related to gastrointestinal movement. Electrogastrogram (EGG) data were obtained in 11 healthy subjects treated by real or sham needle in single-blinded condition for 40 min, including before, during acupuncture treatment, and after removing needle. According to frequency analysis of EGG, median frequency(MF) was increased by real needle in 4 subjects, MF for another 2 subjects were increased by real and sham needle and no trend shift in MF showed in other 5 subjects. Our result showed that acupuncture treatment on Joksamni has some effects on gastrointestinal movement. In this study we have applied both real acupuncture and sham acupuncture on the same subjects. So its blinding was regarded as good but was effective in about 36%~55%. Two subjects were assumed that they showed placebo effects on both real and sham acupuncture. Therefore it is necessary to perform further study on case-control and a larger number of subjects with functional dyspepsia, in order to confirm our findings.

• 대한수의학회지
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• 제40권4호
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• pp.755-762
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• 2000

The aim of this investigation was to examine the effects of osteocalcin, bone-specific alkaline phophatase, estrogen, insulin-like growth factor-I (IGF-I), Ca, P and bone mineral density on osteoporosis induced by ovariectomy in rats. Female Sprague-Dawley 30 rats of three-forth's birth, weighing $215{\pm}10g$, were divided into two groups including the sham operation group(5 heads) and ovariectomy group(25 heads). They were fed normal diets for 2 weeks before the experimental operation and for 8 more weeks after operation. The level of osteocalcin, TALP, BALP, estrogen, bone mineral density and IGF-I were increased in experimental group, but a little increased in sham operation group at same period. The change of rates of osteocalcin, TALP, BALP, estrogen, bone mineral density and IGF-I were significantly higher in experimental group than sham operation group. $Ca^{2+}$ was not changed between two groups and P was significantly decreased in experimental group and Ca/P ratio was higher in experimental group than sham operation group. Body weights were increased in all two groups and growth rate per day was higher in experimental group than sham operation group. However, femur weight I body weight ratio was lower in experimental group than sham operation group.

• 대한한의학방제학회지
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• 제9권1호
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• pp.251-271
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• 2001

In order to investigate the effect of the oral administration of Guirokyisun-Hwan on the estrogen-deficiency osteoporosis induced by ovariectomy in the rats, serum osteocalcin, estradiol, progesterone, follicular-stimulating hormone (FSH), calcium (Ca) and phosphorous (P) levels were monitored. And body weight changes were also monitored throughout whole experimental periods. The results were as follows : 1. Body weight of Control and Treatment group was significantly increased from 6 weeks after ovariectomy but after 2 days of drug administration, body weight of Treatment group were significantly (p<0.01 or 0.05) decreased compared with those of Control group. 2. Serum osteocalcin levels were significantly (p<0.01) increased in control groups compared with those of Sham group but significantly (p<0.01) decreased in the Treatment group. 3. Serum estradiol levels were significantly (p<0.01) decreased in control groups compared with those of Sham group but significantly (p<0.01) increased in the Treatment group. 4. Serum progesterone levels were significantly (p<0.01) decreased in control groups compared with those of Sham group but significantly (p<0.01) decreased in the Treatment groups. 5. Serum FSH levels were significantly (p<0.01) increased in control groups compared with those of Sham group but significantly (p<0.05) decreased in the Treatment group. 6. Serum Ca levels were significantly (p<0.01) decreased in control groups compared with those of Sham group but significantly (p<0.01) increased in the Treatment group. 7. Serum P levels were significantly (p<0.01) increased in control groups compared with those of Sham group and Treatment group. No significant was detected between Control group and Treatment group. According to these results, it is considered that the oral administration of Guirokyisun-Hwan have favorable effect on treatment and prevention of osteoporosis induced by ovariectomy in rats.