• The Korean Journal of Mycology
• /
• v.11 no.4
• /
• pp.147-150
• /
• 1983

To investigate a possibility of producing the antitumor component by shake culture method, the mycelia of Flammulina velutipes were cultured in flasks on a shaker at $26{\sim}28^{\circ}C$ at 180 rpm for seven days. The extract of the mycelia was concentrated under vacuum. The precipitate obtained by adding a three-fold volume of ethanol was centrifugated and freeze-dried after dialysis. The fraction was tested against sarcoma 180 in the mice. The inhibition ratio of the fraction against the tumor was 68.0% at the dose of 20 mg/kg/day for the period of ten days and the tumors in three of the mice were completely regressed. The results showed, therefore, that the antitumor component was produced by the shake culture method.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.20 no.3
• /
• pp.279-286
• /
• 2010

In this paper, we provide a robust track-following controller design method for the stable coarse seek control. Due to the inaccurate velocity control during a coarse seek, the shake of fine actuator is generated and thus a gain-up track-following control is required to complete stably the coarse seek. To this end, a loop gain adjustment algorithm is introduced to estimate accurately the shake of fine actuator. A weighting function can be properly selected from a minimum tracking gain-up open-loop gain, calculated from the estimated shake quantity of fine actuator. A robust tracking gain-up controller is designed by considering a robust $H_{\infty}$ control problem using the weighting function. The proposed design method is applied to the coarse seek control system of an optical rewritable drive and is evaluated through the experimental results.

• Proceedings of the Earthquake Engineering Society of Korea Conference
• /
• 2006.03a
• /
• pp.571-578
• /
• 2006

Research using multiple shake tables present new and unique challenges in controls. Typical single shake table tests with large specimens must cope with significant specimen force feedback that can increase tracking error due to specimen gain, damping, and non-linearity. Multiple shaking tables with distributed specimens can produce cross-coupling forces due to inertial and response effects and forces due to static differential displacements. Although many various control architectures exist, basic simplified techniques can yield excellent results without risk to control stability. Off-line simulation techniques can also prove invaluable for studying system response before the real system is operated.

• Earthquakes and Structures
• /
• v.16 no.3
• /
• pp.321-327
• /
• 2019

This study investigated the possibility of using the recorded micro tremor data on ground level as ambient vibration input excitation data for investigation and application Operational Modal Analysis (OMA) on the bench-scale earthquake simulator (The Quanser Shake Table) for model chimney. As known OMA methods (such as EFDD, SSI and so on) are supposed to deal with the ambient responses. For this purpose, analytical and experimental modal analysis of a model chimney for dynamic characteristics was performed. 3D Finite element model of the chimney was evaluated based on the design drawing. Ambient excitation was provided by shake table from the recorded micro tremor ambient vibration data on ground level. Enhanced Frequency Domain Decomposition is used for the output only modal identification. From this study, best correlation is found between mode shapes. Natural frequencies and analytical frequencies in average (only) 1.996% are different.

• Earthquakes and Structures
• /
• v.16 no.6
• /
• pp.665-677
• /
• 2019

This study utilizes large-scale shake table test to investigate the seismic performance of an isolated bridge with lead rubber bearings crossing an active fault. Two transverse restraining systems with and without shear keys are tested by applying spatially varying ground motions. It is shown that the near-fault span exhibits larger bearing displacement than the crossing-fault span. Bridge piers away from the fault rupture are more vulnerable than those adjacent to the fault rupture by attracting more seismic demand. It is also verified that the shear keys are effective in restraining the bearing displacement on the near-fault span, particularly under the large permanent ground displacement.

• Proceedings of the KSME Conference
• /
• 2001.06c
• /
• pp.361-368
• /
• 2001

This paper introduces optimization techniques to design engine mount properties for passenger vehicle. The design targets are divided into three cases such as optimal positioning of powertrain modes, minimizing vibration of deriver's seat in idling and driving conditions. The proper models, mechanisms of vibration, and characteristics of optimization problems are discussed.

• Smart Structures and Systems
• /
• v.25 no.2
• /
• pp.155-167
• /
• 2020

This paper demonstrates a real-time hybrid substructuring (RTHS) shake table test to evaluate the seismic performance of a base isolated building. Since RTHS involves a feedback loop in the test implementation, the frequency dependent magnitude and inherent time delay of the actuator dynamics can introduce inaccuracy and instability. The paper presents a robust stability and performance analysis method for the RTHS test. The robust stability method involves casting the actuator dynamics as a multiplicative uncertainty and applying the small gain theorem to derive the sufficient conditions for robust stability and performance. The attractive feature of this robust stability and performance analysis method is that it accommodates linearized modeled or measured frequency response functions for both the physical substructure and actuator dynamics. Significant experimental research has been conducted on base isolators and dampers toward developing high fidelity numerical models. Shake table testing, where the building superstructure is tested while the isolation layer is numerically modeled, can allow for a range of isolation strategies to be examined for a single shake table experiment. Further, recent concerns in base isolation for long period, long duration earthquakes necessitate adding damping at the isolation layer, which can allow higher frequency energy to be transmitted into the superstructure and can result in damage to structural and nonstructural components that can be difficult to numerically model and accurately predict. As such, physical testing of the superstructure while numerically modeling the isolation layer may be desired. The RTHS approach has been previously proposed for base isolated buildings, however, to date it has not been conducted on a base isolated structure isolated at the ground level and where the isolation layer itself is numerically simulated. This configuration provides multiple challenges in the RTHS stability associated with higher physical substructure frequencies and a low numerical to physical mass ratio. This paper demonstrates a base isolated RTHS test and the robust stability and performance analysis necessary to ensure the stability and accuracy. The tests consist of a scaled idealized 4-story superstructure building model placed directly onto a shake table and the isolation layer simulated in MATLAB/Simulink using a dSpace real-time controller.

• Earthquakes and Structures
• /
• v.12 no.6
• /
• pp.689-697
• /
• 2017

Nowadays, there are a great number of various structures that have been retrofitted by using different FRP Composites. Due to this, more researches need to be conducted to know more the characteristics of these structures, not only that but also a comparison among them before and after the retrofitting is needed. In this research, a model steel structure is tested using a bench-scale earthquake simulator on the shake table, using recorded micro tremor data, in order to get the dynamic behaviors. Beams of the model steel structure are then retrofitted by using CFRP composite, and then tested on the Quanser shake table by using the recorded micro tremor data. At this stage, it is needed to evaluate the dynamic behaviors of the retrofitted model steel structure. Various types of methods of OMA, such as EFDD, SSI, etc. are used to take action in the ambient responses. Having a purpose to learn more about the effects of FRP composite, experimental model analysis of both types (retrofitted and no-retrofitted models) is conducted to evaluate their dynamic behaviors. There is a provision of ambient excitation to the shake table by using recorded micro tremor ambient vibration data on ground level. Furthermore, the Enhanced Frequency Domain decomposition is used through output-only modal identification. At the end of this study, moderate correlation is obtained between mode shapes, periods and damping ratios. The aim of this research is to show and determine the effects of CFRP Composite implementation on structural responses of the model steel structure, in terms of changing its dynamical behaviors. The frequencies for model steel structure and the retrofitted model steel structure are shown to be 34.43% in average difference. Finally, it is shown that, in order to evaluate the period and rigidity of retrofitted structures, OMA might be used.

• The Journal of the Acoustical Society of Korea
• /
• v.11 no.4
• /
• pp.5-13
• /
• 1992

The vibration of a vehicle, which is caused by and transmitted from the engine, has significant effect on the ride comfort and the dynamic characteristics of the engine mount system has direct influence on the vibration and noise of the vehicle. This paper examines the body shake caused by the engine excitation force on engine key-off of a jeep by experiment and computer simulation using a general purpose mechanical system program, DADS. The computer simulation model consists of the engine, body including frame, and front and rear axles and each axle has right and left tires. The force element between body and suspension is modeled as a combination of suspension spring and damper, and the unsprung mass has roll and pitch motion. The body shake obtained from experiment was compared with the result of computer simulation. Parametric study of the body shake on engine key-off is performed with changing the stiffness of engine mount rubber, the engine mount installation angle and position of engine mounts by using the verified computer simulation model.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.1
• /
• pp.128-134
• /
• 2008

The kinetics of cell growth and Cyclosporin A (Cyc A) production by Tolypocladium inflatum were studied in shake flasks and bioreactors under controlled and uncontrolled pH conditions. In the case of the shake flask, the production time was extended to 226 h and the maximal antibiotic concentration was 76 mg/l. When scaling up the cultivation process to a bioreactor level, the production time was reduced to only 70h with a significant increase in both the cell growth and the antibiotic production. The maximal dry cell weights in the case of the controlled pH and uncontrolled pH cultures in the bioreactor were 22.4g/l and 14.2g/l, respectively. The corresponding maximal dry cell weight values did not exceed 7.25g/l with the shake flask cultures. The maximal values for Cyc A production were 144.72 and 131.4 mg/l for the controlled and uncontrolled pH cultures, respectively. It is also worth noting that a significant reduction was observed in both the dry cell mass and the antibiotic concentration after the Cyc A production phase, whereas the highest rate of antibiotic degradation was observed in the stirred tank bioreactor with an uncontrolled pH. Morphological characterization of the micromorphological cell growth (mycelial/pellet forms) was also performed during cultivation in the bioreactor.