• 유기물자원화
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• 제28권3호
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• pp.27-36
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• 2020

본 연구는 수입유박 대체 자재 개발을 위해 국내 유기자원을 배합하여 발효하는 과정에서 무기성분과 미생물수의 변화를 조사하여 혼합발효 유기질비료의 특성을 구명하고, 제조된 혼합발효 유기질비료의 기존 혼합유박 대체 가능성을 검토하고자 상추 생육에 미치는 영향을 조사하였다. 국내 부산물 자원 미강, 주정박, 참깨박, 어분, 버섯폐배지 등 5종을 혼합비율별로 혼합하고 수분함량 30%, 밀봉조건에서 90일간 발효과정 중의 성분을 분석하여 기존 혼합유박 성분함량 기준에 적합한 혼합발효 유기질비료를 제조하였다. 발효 90일 동안 비료의 pH 변화는 적으며, 수분함량은 발효 60일까지는 34~35%로 유지되다가 90일차에 30~31%로 감소하였다. 총질소 함량은 발효기간 동안 큰 변화 없이 유지되었으나 총탄소 함량은 발효 21일차에 유의적인 차이를 나타냈다. 제조된 혼합발효 유기질비료의 비료성분 (질소, 인산, 칼륨) 함량이 8.7% 이상으로 유기질비료에 함유된 주성분의 최소량 기준에 적합한 것을 확인하였다. 유기질비료 발효과정 중 세균과 방선균의 밀도는 각각 60일, 30일차까지 증가하다 이후 변화가 적었으며 사상균은 증가하는 경향을 나타냈다. 제조된 혼합발효 유기질비료를 시용하여 온실에서 상추를 재배한 시험 결과, 표준 질소시비량 기준 100% 시용시 혼합유박 대비 생육 및 수량이 대등한 결과를 나타냈다. 따라서 지역 내 발생되는 부산물자원으로 제조된 혼합발효 유기질비료는 수입유박을 대체하여 양분관리 자재로 농가 활용이 가능하며 친환경농산물의 안정적 생산에 기여할 수 있을 것으로 기대된다.

• 환경위생공학
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• 제22권3호
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• pp.1-12
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• 2007

본 연구는 인천항으로 유입되는 휴대농산물의 안전성 확보를 위하여 휴대량 초과로 인천본부세관에 유치되는 농산물 등을 검사하였다. 수거 검체는 건고추절편 또는 고춧가루, 곡류, 종실류, 콩류, 견과류, 한약재원료, 참기름 등 총 293건을 대상으로, 품목별로 잔류농약, 곰팡이수, 타르색소, 수단색소 I, II, III, IV, 아플라톡신, 납, 카드뮴, 수은, 비소, 잔류이산화황 분석을 실시하였다. 1. 건고추절편 또는 고춧가루는 잔류농약 동시다성분분석, 곰팡이수, 타르색소, 수단색소 I, II, III, IV 검사를 실시하였고 이중 곰팡이수와 타르색소에서 부적합이 있었다. 2. 종실류는 타르색소 검사에서 불검출이었고, 콩류와 견과류의 아플라톡신 검사결과도 불검출이었다. 3. 곡류는 납, 카드뮴, 수은의 중금속 검사와 흑미의 경우 타르색소 검사를 추가하였고, 쌀 2건, 찹쌀, 율무, 조에서 각 1건씩 납 기준을 초과하였다. 4. 한약재 원료는 납, 카드뮴, 수은, 비소, 잔류이산화황 검사에서 대추 2건과 건강 3건에서 잔류이산화황이 기준을 초과하였고, 건강 1건에서 카드뮴이 부적합하였다. 5. 추석 전에 대거 유입된 참기름의 규격검사 결과는 모두 적합한 것으로 나타났다. 6. 총 293건의 휴대농산물 가운데 건고추 절편 또는 고춧가루 78건, 곡류 5건, 한약재 5건 등 88건에서 규격기준을 초과하여 30% 부적합율을 나타내었다. 시험항목 중에서는 곰팡이수 부적합 76건, 타르색소 1 건, 곰팡이수와 타르색소 동시부적합 1건, 납 5건, 잔류이산화황 4건, 잔류이산화황과 카드뮴 동시부적합 1건으로 나타났다. 7. 중금속 측정결과는 곡류에서 $Pb\;0.000{\sim}0.740mg/kg(0.191{\pm}0.209),\;Cd\;0.000{\sim}0.108mg/kg(0.007{\pm}0.020),\;Hg\;0.000{\sim}4.201mg/kg(0.162{\pm}0.319)$으로 나타났으며, 한약재원료에서는 $Pb\;0.000{\sim}2.480mg/kg(1.130{\pm}0.730),\;Cd\;0.000{\sim}0.761mg/kg(0.044{\pm}0.122),\;Hg\;0.000{\sim}0.157mg/kg(0.022{\pm}0.033),\;As\;0.000{\sim}0.560mg/kg(0.040{\pm}0.082)$, 잔류이산화황은 $2.0{\sim}1459.4ppm(303.1{\pm}324.2)$으로 나타났다.

• 동서간호학연구지
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• 제3권1호
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• pp.17-26
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• 1998

The purposes of this study were to identify the contents, frequency of use, and the rationales of folk remedies frequently used for first aid at home for stomachache, vomiting, strain, food poisoning, vaginal bleeding, or wounds of snake or dog bite, or bee sting. As the method of the study, data were collected by conducting a questionnaire of 185 persons, who filled it out or who were interviewed from May to August in 1998. The collected data was analyzed by using frequency and the rationales for the contents of the folk remedies were described base on literatures. The results were as follows,. 1. The folk remedies for stomachache in frequency order were 'warming the abdomen', 'rubbing the abdomen with a hand', or 'pricking a finger to bleed', which have the rationals. 2. The folk remedies for vomiting in frequency order are 'drinking water of watery radish' and 'pricking a finger to bleed', which have the rationales. 3. The folk remedies for strain in frequency order are 'soaking the affected area into cold water or applying an ice pack' and 'after soaking jasmine in water, making a dough with flour, and then attaching it on the affected area', which have the rationales, Additionally, although the frequencies are low, 'drinking brewed Achyranthis Radix' and 'applying brewed leaves and stalks of Sambuci Cortex' have the rationales. 4. The folk remedies for food poisoning in frequency order are 'drinking black beans and licorice brewed together' and 'eating mung beans', which have the scientific grounds. 5. The folk remedies for bloody discharge in frequency order are 'drinking brewed lotus root' and 'eating boiled chicken stuffed with root of bell-flower'. However, 'drinking brewed Thujae orientalis Folium' has the rationales. 6. The folk remedy for snake bite wounds that has the scientific grounds is 'sucking blood by a person without hurt in the mouth'. However, quite a lot of people apply alum or soy paste, which has no rationales. 7. The folk remedies for the bee sting in frequency order are 'after removing the sting, applying soy paste or sauce' and 'applying saliva'. No rationales for 'applying saliva' have been found. 8. The folk remedy for the dog bite wound that has the rationales is 'applying juice of leaves or stalks of Xanthii Fructus'. However, 85% of surveyed people use remedies that have no scientific grounds, such as 'applying burnt dog hair mixed with (sesame) oil' and 'applying soy paste or sauce'. Various materials are used in folk remedies for first aid treatment as shown above. Some of the folk remedies have the rationales since their medicinal actions have been found. However, the medicinal actions of the majority have not been found, but only known that they are effective, Especially for the materials except botanical drugs, they are used without knowing the effects since their components and pharmacognosies have not been described in any literature. The results of this research may be used for materials to educate the surveyed people or to consult them. Accordingly, the folk remedies that have the rationales are required to continue to study to find out the effects.

• 한국전자파학회:학술대회논문집
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• 한국전자파학회 1999년도 제3회 전자장의 생체영향에 관한 워크숍
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• pp.173-186
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• 1999

Several epidemiological studies have suggested that residential or occupational exposure to power frequency magnetic fields (MF) might increase the risk of cancer. The objective of this study is to elucidate the possible carcinogenic effects of MF exposure using female Sprague-Dawley (Crj:CD)rats. A total of 360rats was randomly divided into 6 groups of 60 rats each. Two groups were served as a negative control (vehicle: sesame oil only) or a positive control (single oral administration of 7, 12dimethylbenz(a)anthracene; DMBA, 90mg/kg body weight at 50-52days of age). Other four groups were simultaneously exposed to 0 (sham-exposed) 7, 70 or 350 $\mu$T(rms), continuous circularly polarized 50HzMF, 22 hrs/day, 7 days/weeks for 30weeks from 8weeks of age. Experiment was conducted under SPF condition and in a blinded manner, Ten animals in each grout were served as satellite animals and their several hormonal concentrations in sera, such as melatonin and prolactin, collected at the midnight were measured. In addition, complete histopathological examination were performed in other 50 animals per each group. In the positive control group, the first mammary nodule was palpated at the 7th weeks of experiment in 5 out of 59 animals. Afterward, the incidence of palpable mammary nodules increased steadily and reached at 76% and 98% of live animals at 14weeks and the end of experiment in sham and 350$\mu$ T-exposed groups were not significantly different from those in the sham-exposed and negative control groups. Histopathologocally, most of palpable nodules were mammary tumors. The incidences of animals with mammary tumors per animals survived ant the end of experiment were 4.1 and 100% in the negative and positive control groups, and 0.0, 6.0, 8.0 and 6.0% in the sham-, 7, 70and 350 $\mu$T-exposed groups, respectively. These incidences in three MF-exposed groups were not significantly different from those in both the sham- exposed and negative control groups. Based on these results, it was not supported that continuous circularly polarized 50 Hz magnetic fields at up to 350$\mu$T affect the incidence of spontaneous mammary tumors in female SD rats under the present experimental conditions.

• Asian-Australasian Journal of Animal Sciences
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• 제20권9호
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• pp.1407-1416
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• 2007

Ten, first lactation, 87.5%HF dairy cattle were used to investigate effects of long-term administration of recombinant bovine somatotropin (rbST) on nutrient uptake by the mammary gland at different stages of lactation. Measurements of arterial plasma concentrations and arterial-venous differences of metabolites across the mammary gland were performed in combination with measurment of mammary blood flow to estimate the mammary uptake. Animals in experimental groups were injected subcutaneously every 14 days from day 60 of lactation with a prolonged-release formulation of 500 mg of rbST (POSILAC, Monsanto, USA) or with sterile sesame oil without rbST in the control group. During early lactation, the milk yield of rbST-treated animals was higher than that of the control animals (p<0.05). The peak milk yield in both groups of animals declined from the early period of lactation with progression to mid- and late-lactation. No significant changes were observed in the concentration of milk lactose, while the concentrations of milk protein significantly increased as lactation advanced to mid- and late-lactation in both groups. Milk fat concentrations were significantly higher in rbST-treated animals than in control animals, particularly in early lactation (p<0.05). Mammary blood flow (MBF) markedly increased during rbST administration and was maintained at a high level throughout lactation. The mean arterial plasma concentrations for glucose and acetate of rbST-treated animals were unchanged. The net mammary glucose uptake of rbST-treated animals increased approximately 20% during early lactation, while it significantly decreased (p<0.05), including the arteriovenous differences (A-V differences) and extraction ratio across the mammary gland, as lactation advanced to mid- and late-lactation. A-V differences, mammary extraction and mammary uptake for acetate increased during rbST administration and were significantly higher (p<0.05) than in the control animals in early and mid-lactation. Mean arterial plasma concentrations for ${\beta}$-hydroxybutyrate and free glycerol were unchanged throughout the experimental periods in both groups. A-V differences and extraction ratio of ${\beta}$-hydroxybutyrate across the mammary gland did not alter during rbST administration. Mean arterial plasma concentrations for free fatty acids ($C_{16}$ to $C_{18}$), but not for triacylglycerol, increased in rbST-treated animals and were significantly higher than in control animals during early lactation (p<0.01). These findings suggest that an increase in MBF during rbST administration would not be a major determinant in the mediation of nutrient delivery and uptake by the mammary gland for increased milk production. Local changes in biosynthetic capacity within the mammary gland would be a factor in the utilization of substrates resulting in the rate of decline in milk yield with advancing lactation.

• 한국식품조리과학회지
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• 제26권5호
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• pp.530-536
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• 2010

이 연구에서는 단백 분해 작용이 있는 키위와 무화과를 과실 자체로 조리에 이용할 때 연육작용이 있는지 살펴보았다. 또한 우리나라 조리방법에 의거하여 양념과 부재료의 영향을 측정하였다. 과실의 단백 분해 효소활성도를 측정한 결과 키위의 경우 36,513 uM/g fruit이었고, 무화과의 경우 24,131 uM/g fruit으로 나타났다. 고기 조리시에 과실을 각각 3%, 5%, 10% 첨가하여 관능검사를 실시한 결과 키위 3~5%와 무화과 5%가 가장 효율적인 첨가량으로 평가되었다. 키위와 무화과를 혼합 사용할 때의 연육에 관한 관능검사를 실시한 결과 키위만을 5% 첨가한 경우 가장 효과적인 것으로 나타났으며 키위와 무화과를 3:0, 2:1, 1:1로 섞은 처리구간의 유의적인 차이는 보이지 않았다. 연육제 처리시간별 연육효과를 관찰한 결과 냉장온도에서 6시간 방치하여 조리할 때 연도와 기호도가 우수한 것으로 나타났다. 온도에 의한 영향을 보면 $25^{\circ}C$ (상온)에서 전처리하는 경우가 $4^{\circ}C$ 처리보다 더 좋은 효과를 나타내었다. 기본양념인 간장, 설탕, 올리고당, 참기름이 첨가된 고기용 과일 sauce의 pH는 무화과의 경우 4.64였고 키위의 경우 4.23이었다. 간장과 올리고당, 맛술의 첨가는 과실 sauce의 역가를 감소시키는 경향을 보여주었다. 갈은 과실 sauce에 양념, 마늘, 생강, 양파, 파 등을 첨가한 sauce의 pH는 무화과의 경우 4.71, 키위의 경우 4.43이였다. 다진 마늘, 다진 파, 갈은 양파, 생강, 배즙은 모두 단백분해활성을 증진시키는 것으로 나타났다. 이렇게 제조된 과실 연육 sauce에 의한 소고기는 연도, 기호도, 바람직하지 않은 과실 맛, 바람직하지 않은 신맛, 모두에서 과실을 넣지 않은 일반 소스에 의한 소고기보다 더 우수한 것으로 나타났다.

• 한국수정란이식학회지
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• 제28권2호
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• pp.157-162
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• 2013

Methoxychlor (MXC) was developed to be a replacement for the banned pesticide DDT. HPTE [2,2-bis (p-hydroxyphenyl)-1,1,1-trichloroethane], which is an in vivo metabolite of MXC, has strong oestrogenic and anti-androgenic effects. MXC and HPTE are thought to produce potentially adverse effects by acting through oestrogen and androgen receptors. Of the two, HPTE binds to sex-steroid receptors with greater affinity, and it inhibits testosterone biosynthesis in Leydig cells by inhibiting cholesterol side-chain cleavage enzyme activity and cholesterol utilisation. In a previous study, MXC was shown to induce Leydig cell apoptosis by decreasing testosterone concentrations. I focused on the effects of MXC on male mice that resulted from interactions with sex-steroid hormone receptors. Sex-steroid hormones affect other organs including the kidney and liver. Accordingly, I hypothesised that MXC can act through sex-steroid receptors to produce adverse effects on the testis, kidney and liver, and I designed our experiments to confirm the different effects of MXC exposure on the male reproductive system, kidney and liver. In these experiments, I used pre-pubescent ICR mice; the puberty period in ICR mice is from postnatal day (PND) 45 to PND60. I treated the experimental group with 0, 100, 200, 400 mg MXC/kg b.w. delivered by an intra-peritoneal injection with sesame oil used as vehicle for 4 weeks. At the end of the experiment, the mice were sacrificed under anaesthesia. The testes and accessory reproductive organs were collected, weighed and prepared for histological investigation. I performed a chemiluminescence immune assay to observe the serum levels of testosterone, LH and FSH. Blood biochemical determination was also performed to check for other effects. There were no significant differences in our histological observations or relative organ weights. Serum testosterone levels were decreased in a dose-dependent manner; a greater dose resulted in the production of less testosterone. Compared to the control group, testosterone concentrations differed in the 200 and 400 mg/kg dosage groups. In conclusion, I observed markedly negative effects of MXC exposure on testosterone concentrations in pre-pubescent male mice. From our biochemical determinations, I observed some changes that indicate renal and hepatic failure. Together, these data suggest that MXC produces adverse effects on the reproductive system, kidney and liver.

• Clinical and Experimental Reproductive Medicine
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• 제31권2호
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• pp.119-131
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• 2004

연구 목적: 난소에서 분비되는 스테로이드 호르몬인 에스트로젠과 프로게스테론은 포유동물의 생식기관 발달과 정상적인 생식 기능, 수정과 배아의 착상에 중요한 역할을 한다. 특히 에스트로젠은 자궁내액을 내강으로 분비하여 자궁부종 기작에 중요한 역할을 한다. 자궁내액은 정자의 수정능력 획득과 착상전 배아의 발달에 매우 중요하다. Aquaporin (AQP)은 막관통 물수송 단백질로서 여러 조직에 넓게 분포되어 있으며, 세포간 또는 상피세포간 물의 이동에 중요한 역할을 한다. 본 연구에서는 생쥐 자궁에서 스테로이드 호르몬에 의해 조절되는 자궁내액의 이동에 AQP 유전자가 관여하는지를 알아보았다. 연구 재료 및 방법: 난소 절제술을 시행한 생쥐에 스테로이드 호르몬을 피하주사하고 6, 12, 24시간 간격으로 자궁조직을 적출하였다. 대조군은 sesame oil만을 주사한 후 6시간째에 수획한 자궁조직을 사용하였으며, 실험군은 시간대별과 스테로이드 처리별로 채취한 자궁조직에서 역전사중합효소반응을 수행하였다. 역전사중합효소반응을 통해 막관통 단백질인 AQP-4, -5, -8 mRNA의 발현양상을 살펴보았다. 또한 mRNA의 위치를 살펴보기 위해 laser microdissection을 이용하여 RT-PCR을 수행하였다. 마지막으로 자궁조직내에서의 단백질 발현 부위를 관찰하기 위해 면역조직화학염색을 실시하였다. 결 과: AQP-4, -5, -8 mRNA은 프로게스테론을 처리한 군보다 에스트로젠을 처리한 군에서 많이 발현되었으며, 에스트로젠을 주사한 지 6시간째 발현정도를 대조군과 비교할 때 AQP-4, -5, -8 mRNA가 각각 7.9배, 2.8배, 3.8배로 나타났다. AQP-4, -5, -8 mRNA는 간충조직보다 자궁내 상피조직에서 스테로이드 호르몬의 영향을 받아 발현양상의 차이가 나타났으며, 주로 에스트로젠의 영향을 받아 발현이 증가하였다. AQP-4 단백질은 에스트로젠을 24시간 처리한 후 프로게스테론을 처리한 군의 자궁내 상피조직에서 많이 발현되었으며, AQP-5와 -8 단백질은 에스트로젠을 처리한 군의 자궁내 상피조직에서 발현이 증가하였다. 결 론: 이상의 결과를 통해 AQP-4, -5, -8은 주로 에스트로젠에 의해 자궁내 상피세포에서 발현이 증가되는 것으로 보아 에스트로젠의 영향하에 일어나는 자궁내액의 이동으로 인한 자궁부종기작에 이동통로로서 관여하는 것으로 사료된다.

• 한국식생활문화학회지
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• 제28권1호
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• pp.78-88
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• 2013

Marinated beef galbi is a traditional Korean dish cooked with soy sauce, pear juice, onion, sesame oil, and sugar. However, there are many differences in beef galbi, including flavor and physicochemical aspects, depending on cooking conditions. Therefore, the physicochemical characteristics of marinated beef galbi prepared through various recipes was evaluated for its effects on pH, texture, aging, proteolysis, heating conditions, cooking time, and flavor compounds (pyrazines, IMPs, or FAAs). There were significant differences in salt concentration (0.8~3.03%), pH (4.89~6.22), and solid soluble contents (1.34-6.31 Brix) between recipes in this study. In the Pearson assay for sensory evaluation, overall preference correlated well with texture (a well-known sensory attribute in meat evaluation). Controlling the pH of meat through soaking in lemon solution, alkali water, phosphate, and baking powder solution, improved water holding capacity as much as 9 to 15% compared with the control. The myofibril index (MFI) of marinated meat stored at $4^{\circ}C$ increased 32% with 24 hours of aging and reached 39% at 48 hours of aging, and its fragmentation was observed through microscopy. SDS-PAGE showed hydrolysis of acid-soluble collagen by the pear juice, possibly related to meat tenderness. On the basis of surface temperature, the cooking time was estimated to be 8 minutes with pan heating at $170^{\circ}C$, 6 minutes at $270{\sim}300^{\circ}C$, and 4 minutes with charcoal at $700{\sim}900^{\circ}C$. Different pyrazine compounds, such as 2-methyl-3-phenylpyrrol(2,3-b) pyrazine (the typical product of the browning reaction) was mainly detected, and IMP (one of the main taste compounds in beef) was in higher amounts with the charcoal treatment, potentially related to its flavor preference among treatments. Our results demonstrate an effective case study and cooking system for beef galbi.

• 한국식품조리과학회지
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• 제2권1호
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• pp.1-10
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• 1986

This sudy was conductted for the purpose of determining the optimal amounts of soy tauce, soy bean paste and hot pepper paste, in 3 different Korean soups and comparing the effect of condiments such as salt, soy sauce, black pepper, and Mono Sodium Glutamate, on the taste of two basic Korean Stocks, The results estimated by Palatability Test were as follows: 1. The average NaCl contents of two kinds of commercial soysauce were 15.9%, 25.6%, respectively. 2. The NaCl contents of 2% sardine stock, and 19% beef stock were 0.16~1.17%, 0.17~0.18% respectively. 3. @ According to Saltiness in sardine stock, there was no difference between the soy sauce-added group and the salt-added group. However, for the removing effect of Off-flavor, and Overall Taste, the soy sauce-group had higher score than salt-added group respectively. (p>.0.1) ⓑ In beef stock, there was no difference between the soysauce-added group and the salt-added group in Saltiness test. The soy sauce-added group had higher score in removing effect of Off-flavor (p>.0.1). On other hand, in Overall Taste, the salt- added group was more effective than the soy sauce-added (p>.0.1). 4 @ According to Saltiness test in beef stock, there was no difference between the black pepper-added and non-added group. However, for the removing effect of Off-flavor and, Overall Taste, the added group had higher score than the non-added group, respectively. (p>.10, p>.0.5) ⓑ Also, according to Saltiness test in beef stock, there was no diffetrence between the MSG-added group and non-added group. However for the removing effect of Off-flavor, and Overall taste, the MSG-added group was more effective than the non-added group. (p>.01) 5. The best recipes of soybean sprouts soup for S persons, in this experiment, were soybean sprouts 180 g, sardine 28 g, soysauce 1/2 T.S., salt 7 g, green onion 15 g, choppedgarlic It. s., against 7 C of water, and total cooking time was 20 minutes. 6. The best recipes of clear-beef soup were beef 200 g, radish 200 g, tangle weed 10 g, sesame oil 1/2 t.s., green onion 15 g, chopped garlic 1 t.s., black Pepper 1/8 1.s., and soy sauce 1 T.S., salt 7 g or soy sauce 2 T S., salt 3.5 g. The total cooking time was minutes. 7, The best recipes of soybean pasted Chinese cabbage soup were Chinese cabbage 300 g, sardine 28 g, green onion 15 g, chopped garlic 1 t.s., and bean paste 50 g, hot pepper paste 13 g, salt 3g or bean paste 65 g, hot pepper paste 16 g, salt 1.5 g. And the total cooking time was 25 minutes. 8. Soy sauce, black pepper, M.S.G., bean paste and hot pepper paste were effective on removing Off-flavor of experimental soups.