• 한국동물학회지
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• 제35권1호
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• pp.96-101
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• 1992

전기영동에 의하여 진도개와 삽사리의 혈청 단백질을 분석하였다. 이들의 혈청 단백질 전기영동상은 5개의 분획으로 나눌 수 있었다. 진도개의 총 혈청단백질은 8.40$\pm$1.63(g/dl)이었으며 albumin과 globulin의 비(A/G)는 0.81 $\pm$ 0.25이었다. 삽사리의 총 혈청단백질은 8.23 $\pm$2.20(g/dl)이었고 A/G는 0.78 $\pm$ 0.23이었다. 연령에 따라 Y-globulin의 양이 가장 크게 변했으며 진도개와 삽사리의 albumin과 globulin의 비는 다른 품종의 개들보다 낮았다.

• 대한한의학회지
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• 제30권2호
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• pp.63-78
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• 2009

Objective: The purpose of experimental study was to prove the effects of Boyangmakseong-bang (BYMSB) treatment on cBSA-induced in a MN mouse model. Methods: We divided mice into 4 groups. The Normal group had no treatment. We used cBSA and induced MN mouse model to the other 3 groups. The Control group was treated with cBSA (9mg/kg i.p) only. The second group, named 'BY-250', was treated with cBSA (9mg/kg i.p) and BYMSB extract (250mg/kg, p.o). The third group, named 'BY-500', was treated with cBSA (9mg/kg i.p) and BYMSB extract (500mg/kg, p.o). After cBSA and BYMSB extract treatment for 4 weeks, the increase in percentage of body weight, proteinuria, serum albumin, total cholesterol, creatinine and BUN of all groups were measured. The CD3+, CD19+, CD4+, CD8+ cell levels of spleen of all groups were analyzed. IgA, IgG, IgM, IL-$1{\beta}$, TNF-${\alpha}$, IL-6 and IFN-${\gamma}$ levels of all groups were gauged. H&E staining, immunofluorescence staining and electron microscopy of kidney were observed. Results: BYMSB showed significant decrease in the 24hrs proteinuria, serum total cholesterol, serum IgG levels and BUN levels, and showed significant increase in the serum albumin levels compared with the control group. BYMSB showed increase in the increasing percentage of body weight and IFN-${\gamma}$ levels compared with the control. BYMSB showed decrease in the CD3+ T cells, CD4+ Th cells, IL-$1{\beta}$, TNF-${\alpha}$ and IL-6 levels, but did not show significant change compared with the control. BYMSB showed considerable decrease in the thickening of the GBM on H&E staining, deposition of IgG on immunofluorescence staining and deposition of electron-density on electron microscopy of kidney compared with the control. Conclusions: According to the above results, it is suggested that BYMSB decreases the symptoms of MN induced by cBSA in a mouse model. Therefore BYMSB seems to be applicable to MN in clinical practice.

• 대한수의학회지
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• 제11권2호
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• pp.145-148
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• 1971

Serum Samples from adult of Korean cattles including 40 females and 20 males were analyzed by sodium salt precipitation and colorimetric method in the purpose of the determination of total serum protein, albumin, globulin, ${\alpha}$-globulin, ${\beta}$-globulin and ${\gamma}$-globulin. The results obtained arc summarized as follows: 1. Mean value of total serum protein showed a slight variation from 7.6%, and its regional and sex differences were not found to be significant. 2. Contents of albumin in serum showed lower level than that of globulin as low level of A/G ratio 0.4 in proportion. 3. Contents of Serum ${\alpha}$-globulin showed 1.4w/v% and $1.51{\pm}0.46$w/v% in each group of female, and $1.31{\pm}0.26$w/v%, in the group of male. 4. Contents of serum ${\beta}$-globulin showed 1.74w/v%, 1.95w/v%, in each group of female, and 1.82w/v% in the group of male. 5. Contents of serum ${\gamma}$-globulin showed 2.32w/v%, 2.30w/v% in each group of female, and 2.30w/v%, in the group of male.

• 대한임상검사과학회지
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• 제38권2호
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• pp.111-116
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• 2006

One of the cardinal findings of the renal diseases is proteinuria, which appears in the early phase of kidney diseases and is very important in diagnosis, prognosis and decision making in the treatment process and results of the treatment. The study subjects were 126 patients who visited the nephrology department of Kangbuk Samsung Hospital. Serum was requested for urine protein electrophoresis. Total protein was measured with Bayer Advia 1650 (Biuret). Quantitation of each fraction was done by multiplying the percentage of each fraction by the total protein. Serum creatinine and BUN were also measured with Bayer Advia 1650 (Jaffe and Urease). Serum protein EP was done with REP(rapid electrophoresis) using Helena Kit reagents (REP Ultra SPE Kit, Ponceau S stain, Acetic acid, Methanol, EP Control). Concentrated urine was used for urine protein EP. The SPSS package was used for statistics analysis. Percentage and quantitation of the level of albumin in renal diseases were significantly lower than those in healthy controls. Total protein was correlated with albumin. In terms of proportion, ${\alpha}1$-globulin, ${\alpha}2$-globulin, ${\beta}$-globulin, and ${\gamma}$-globulin fractions were increased in the disease group. But, in the quantified level, ${\alpha}2$-globulin was increased and ${\beta}$-globulin and ${\gamma}$-globulin were decreased. ESRD patients showed an increased secretion of high molecular proteins in urine protein EP. A decreased level in serum total protein correlated with the decreased level of serum albumin and the total amount of urine total protein. This study revealed the variety in the level of serum and urine proteins and their subgroups by EP.

• Journal of Nutrition and Health
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• 제7권4호
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• pp.33-37
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• 1974

Changes in the concentration of total protein, albumin, ${\alpha}_1-,{\alpha}_2-,{\beta}-,$ and ${\gamma}-globulin$ in Serum from 138 healthy, normal pregnant woman were studied by the method of cellulose-acetate electrophoresis. 1. The concentration of total serum protein decreased gradually during the first 7 month, and showed a tendency to increase thereafter. 2. The concentration of serum albumin showed a steady continuous fall untill term. 3. During pregnancy,${\alpha}_1$ and ${\beta}-globulin$ value rose, ${\gamma}-globulin$ value fell and ${\alpha}_2-globulin$ value showed no significant change.

• 한국식품위생안전성학회지
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• 제2권4호
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• pp.201-208
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• 1987

The effects of ginseng on the blood biochemical components of heavy metals poisoning in mice were examined and following results were obtained. Albumin was decreased slightly in the heavy metal administration groups. But Serum Calcium, Glucose were almost the same as that of control group during the period. Heavy metal poisoning mice showed low levels of serum Total protein and A/G ratio. They also showed high levels of serum GOT, GPT, BUN, Cholesterol, Creatinine and Triglycerides. However, the administration of ginseng significantly inhibited the elevation of them.

• 대한한의학회지
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• 제21권3호
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• pp.199-208
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• 2000

Objectives : In order to investigate the toxicity of rats after oral administration of Eumcheonyijin-tang extract. Methods : The experimental animals were subdivided into control, short term administration group, and long term administration group. With changes of gross appearance, the histological changes of liver and kidney were observed. Blood chemical indexes used in this study were AST, ALT, total bilirubin, albumin, BUN and creatinine in serum. Results : In the long term administration group, histological changes were detected in the liver as centrolobular disposition of fatty tissue(adipose cell), and in serum test, AST, ALT increased at 21 days after administration group, serum total bilirubin were increased 21, 28 and 35 days after administration group. So it seems to induce toxicity. Kidneys of the long term administration group revealed histological changes : increasing of connective tissue and pyknosis of glomerulus cell were observed at 28 days after administration group, and in serum test, significant changes of albumin, BUN, and creatinine were admitted. So it seems to induce toxicity. Conclusions : In long term administration of Eumcheonyijin-tang toxicity was induced.

• Journal of Nutrition and Health
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• 제26권2호
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• pp.189-195
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• 1993

This study conducted to investigate the effect of school lunch prgram on the blood composition of 50 children at the age 11 to 12 years old. Concentration of serum total protein, albumin, zinc and copper were measured at the begining of school lunch program(pre-feeding group) and at the one year later taken school lunch program(post-feeding group). Serum total protein and albumin concentrations in post-feeding group were higher than those in pre-feeding group, but showed no significant difference between groups. While serum zinc and copper concentrations in post-feeding group were significantly lower than those in pre-feeding group. Results indicated that school lunch program effective for improvement of potein nutrition in school children and it is nessessary futher investigation to disease the concentrations of serum zinc and copper in this age.

• Journal of Periodontal and Implant Science
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• 제24권1호
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• pp.178-184
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• 1994

This study was undertaken to examine the protein content of GCF and serum from noraml population in order to standardize the sample loading on SDS/PAGE gels. The resulats were as follows ; 1. The protein concentration of serum was not different between normal group and diseased group. 2. In GCF, the bands of lower molecular weight than albumin were heavily stained, but in serum, the protein bands of higher molecalar weight were found. 3. The profile of protein in normal GCF was characterized by heavily staining bands at 77, 66, 55, 26 KDa corresponding to the positions of transferrin, albumin, heavy and light chains of Ig G. Also 47, 37 KDa nonplasma proteins were found.

• Journal of Nutrition and Health
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• 제24권6호
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• pp.506-515
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• 1991

Serum concentration of selenium were measured ; Studying 36 patients with Hepatoma. 19 with Hepatitis 15 with Liver cirrhosis and 67 Normal control to discover the correlation between serum selenum and cancer. And it have tested also the serum protein and albumin to compare the nutrition status by researching the same persons above The results of those two experiments are as follow : 1) The mean of each group of patients resulting from mearsuing the serum protein content showing 6.91$\pm$0.10g/dl in the patients with hepatoma 7.05$\pm$0.17g/dl in the ones with hepatitis 6.42$\pm$0.13g/dl in the ones with liver cirrhosis and 7.46$\pm$0.06g/dl in the normal control is all being within the normal range such as 6.0-0.8(g/dl) while the distribution is revealin 10.5%, 13.3% in the patients with hepatitis and liver cirrhosis which are below the normal range and 47.2%, 96.9%, 80.0% in the low range of normal one ; therefore in could see that patientsw compared with normal control are in low level in the serum protein content 2) The mean based on the consquence of serum albumin measurement is 3.74$\pm$0.11g/dl. 4.26$\pm$0.13g/dl 4.73$\pm$0.06g/dl in the patients with hepatoma hepatits and normal control which are in between the normal range such as 3.5-5.5(g/dl) except the patients with liver cirrhosis(3/05$\pm$0.12g/dl) while the distribution of the patients with hepatoma an liver cirrhosis is showing 27.8% 73.3% which are below the normal one and 55.5%, 52.6% and 26.7% in the patients with hepatoma hepatits and liver cirrhosis which are in the low area of normal range ; as a result It could find that patients. compared with the normal control are in low level in serum albumin measurement. 3) The mean consulting the serum concentration is 430.86$\pm$26.06$\mu\textrm{g}$/1, 192.37$\pm$18.15$\mu\textrm{g}$/1 186.73$\pm$23.99$\mu\textrm{g}$/1, 196.94$\pm$8.94$\mu\textrm{g}$/1 in the patients with hepatoma hepatitis liver cirrhosis and noramal control ; it shows 2.19 times increasing of serum selenium concentra-tion in the patients with hepatoma 'compared with the normal control and patients with hepatoma spread out at a still higher range in serum selenium concentration test. 4) The serum selenium concentration is not quite dependent on sexes and doesn't show any correlation with age, serum protein, serum albumin. The nutrition status of patients, rested on the results of those experiments, stays in low level, which is caused by that they are in low stage of serum protein and serum albu¬min. In serum selenium concentration, patients with hepatitis and liver cirrhosis, specially, show the condition as good as normal control's; while patients with hepatoma are indicating a significant increasing compared with the three groups, which can be reasoned into that there is a possibility for the patients to take selenium and chemotherapy or radiation treatment, and have high serum selenium concentration by poor excreting of seleno methionine, which is caused by low serum protein content And another thing can be reasoned is that the difference in origination mechanism hetween korean and western people. As a result, It showed that the correlation between serum selenium and seses, age, serum protein, and serum albumin neither exists nor affects on each other.