• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.582-586
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• 2006

This experiment was conducted to investigate the dietary effect of garlic powder (GP) and copper (Cu) on laying performances and the cholesterol content and quality characteristics of eggs during storage in laying hens. A total of one hundred and eighty, 50-wk-old, Hy-Line Brown layers were divided into 6 groups with 3 replicates per group (10 layers per replicate) and fed one of six diets containing GP 0%, GP 1%, GP 3%, GP 5%, Cu 200 ppm, or GP 3%-Cu 200 ppm for 5 wks. There were no differences in the laying performances and feed intakes bertween treatments. Eggshell strength, eggshell thickness and yolk color were also not affected by feeding of GP and Cu. With increasing dietary GP, Haugh unit was linearly increased after 2 wk of storage (p<0.05). The levels of serum total cholesterol in hens fed diets containing GP or Cu were lower than that of the control (p<0.05), but high density lipoprotein-cholesterol was not influenced by dietary GP or Cu. The content of egg yolk cholesterol from hens fed diets containing GP or Cu was significantly decreased from that of the control, except for the GP 1% group. Based on the results of this experiment, the decrease of Haugh unit during storage was alleviated by feeding of GP. The feeding of GP or Cu alone and in combination altered the cholesterol fractions in serum and reduced the content of egg yolk cholesterol in laying hens.

• Preventive Nutrition and Food Science
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• v.12 no.1
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• pp.23-28
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• 2007

The purpose of this study was to investigate the relationships of obesity, serum antioxidant mineral concentrations and blood lipid parameters in middle school students. Subjects were assigned to two groups, obese (BMI$\geq$25, 32 boys, 24 girls) and normal group (18.5

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.1
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• pp.99-105
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• 2000

The purpose of this study was to evaluat the Zn, Cu, Mn and Ni status and correlation of their concentrations in serum, urine, hair and fingernail in female college students. As the results are follows; The mean age of subjects was 22.5 years and height, weight and BMI were 160.1 cm, 51.0 kg and 20.0 respectively. The daily intake of energy 1769.5 kcal and the ratio of carbohydrate, fat and protein for energy is 60 : 20 : 15. The daily intake of energy (88.5%), vitamin B2 (86.1%), Ca (75.4%), Fe (58.3%) and Zn (63.0%) of subjects did not reach to Korean Recommended Dietary Allowance (RDA). The daily mean intake of Zn, Cu, Mn and Ni were 7.56 mg, 2.30 mg, 3.81 mg and 0.18 mg respectively. The concentrations of Zn in serum, urine, hair and nail were 85.6$\mu\textrm{g}$/dL, 391.2$\mu\textrm{g}$/day, 174.6$\mu\textrm{g}$/g and 102.4$\mu\textrm{g}$/g respectively and those Cu were 84.2$\mu\textrm{g}$/dL, 56.6 $\mu\textrm{g}$/day, 20.3$\mu\textrm{g}$/g and 4.3$\mu\textrm{g}$/g respectively. The concentrations of Mn in serum, urine, hari and nail were 0.2$\mu\textrm{g}$/dL, 1.1$\mu\textrm{g}$/day, 1.8$\mu\textrm{g}$/g and 1.6$\mu\textrm{g}$/g respectively and those of Ni were 0.6$\mu\textrm{g}$/dL, 24.5$\mu\textrm{g}$/day, 3.5$\mu\textrm{g}$/g and 3.1$\mu\textrm{g}$/g respectively. The daily intake of Mn showed the positive correlations with concentration of Mn in hair (p<0.05) and the daily intake of Ni was positively correlated with urinary exretion (p<0.05) and hair contents (p<0.001) of Ni. The more detailed studies about these trace mineral status should be required.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.6
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• pp.402-409
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• 2001

Different bioligands carrying synthetic adsorbents have been reported in the literature for protein separation, We have developed a novel and new approach to obtain high protein ad-sorption capacity utilizing 2-methacrylamidohistidine(MAH) as a bioligand. MAH was synthe-sized by reacting methacrylocholride and histidine, Spherical beads with an average size of 150-200㎛ were obtained by the radical suspension polymerization of MAH and 2-hydrosyethyl-methacrylate(HEMA) conducted in an aqueous dispersion medium. p(HEMA-co-MAH) beads had a specific surface area of 17.6㎡/g . Synthesized MAH monomer was characterized by NMR. p(HEMA-co-MAH) beads were characterized by swelling test, FTIR and elemental analysis. Then Cu(II) ions were incorporated onto the beads and Cu(II) loading was found to be 0.96 mmol/g.These affinity beads with a swelling ration of 65% and containing, 1.6 mmol MAH/g were used in the adsorption/desorption of human serum albumin(HSA) from both aqueous solutions and hu-man serum. The adsorption of HSA onto p(HEM-co-MAH) was low(8.8 mg/g). Cu(II) chelation onto the beads significantly increased the HSA adsorption (56.3 mg/g). The maximum HSA ad-sorption ws observed at pH 8.0 Higher HSA adsorption was observed from human plasma(94.6 mgHSA/g) Adsorption of other serum proteins were obtained as 3.7 mg/g for fibrinogen and 8.5mg/g for γ-globulin. The total protein adsorption was determined as 107.1mg/g. Desorption of HSA was obtained using 0.1 M Tris/HCl buffer containing 0.5 M NaSCN, High desorption rations(up to 98% of the adsorbed HSA) were observed. It was possible to reuse Cu(II) chelated-p(HEMA-co-MAH) beads without significant decreases in the adsorption capacities.

• Journal of the Korean Society of Food Science and Nutrition
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• v.16 no.2
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• pp.63-68
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• 1987

The effects of various levels of copper(Cu) intake on the concentrations of copper, iron (Fe) and 3inc(Zn) in rat tissues were studied in growing rats. For different groups the drinking water was supplemented with 0(control), 25, 50, 100 and 200ppm Cu(as copper sulphate) for 1 day respectively. All animal groups were fed with the control diet (Cu contents, 12.8%mg/kg diet) during the experiment. At the end of the 4 week experiment, body weight gain was slightly lower in the Cu supply groups than in control group. Liver and serum Cu were significantly higher in 50, 100 and 200ppm Cu of male and in 200ppm Cu of female than in control groups. Spleen Cu was significantly increased by the supplementation of Cu. Liver and heart Fe of male and heart Fe of female were increased by incresing supplementary Cu levels. In 50ppm Cu group, liver, spleen and kidney Fe of female increased but the others did not. Fe of tissues was different in male and female rats according to Cu levels supplied. Serum Zn of male and female was significantly lower in 50, 100 and 200ppm Cu groups than in control and 25ppm Cu groups. When supplemented with Cu levels there were no significant differences among groups for liver, kidney, spleen and heart Zn as well as heart and kidney Cu.

• Journal of Animal Science and Technology
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• v.63 no.2
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• pp.295-304
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• 2021

This study was aimed to investigate the Cu bioavailability, growth response, digestibility of nutrients, and blood metabolites of broiler chicks fed CuSO4 in nano or common forms. A total of 720 broiler chickens were distributed between eight treatments according to a completely randomized design. There were 8 treatments and 6 replicates in each treatment with 15 birds/replicate. The treatments were divided into common copper sulfate at the doses of 16 ppm, 40 ppm, 80 ppm, and 120 ppm (INO) and hot-melt extruded copper sulfate at the doses of 16 ppm, 40 ppm, 80 ppm, and 120 ppm (HME-Cu). The experiment was operated for 35 days in 2 phases (phase 1, d 0 to 14; and phase 2, d 15 to 35). No significant differences were shown in growth performance, feed intake, FCR, and nutrient digestibility among the treatments. The concentration of Cu in the serum was increased in the HME-Cu broilers compared with the INO broilers at phase 2. A linear increase was observed in the concentration of Cu in the liver in broilers fed INO diets, however, no significant differences were observed by the supplementation of HME-Cu levels. The linear increase was detected in the content of Cu in excreta in the INO and HME-Cu treatments by increasing the dietary Cu content. The HME-Cu treatments showed a lower Cu concentration in the excreta compared with the INO treatments. The higher bioavailability of Cu in HME form can decrease the recommended dose of Cu in broiler diets.

• Korean Journal of Clinical Laboratory Science
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• v.41 no.1
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• pp.31-36
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• 2009

The exposure of human beings to toxic trace metals (Cu, Zn) continues to be an important public health issue and concern. This study was conducted to assess the exposure to trace metals (Cu, Zn) in the general Korean population by inductively coupled plasma mass spectrometry (ICP-MS) and Atomic absorption spectrometry (AAS). Serum samples were obtained from 40 healthy volunteers. Specimens were collected in special container and we applied sample processing to minimize contamination. We used ICP-MS and AAS to analyze simultaneously the concentration of metals including copper, zinc. Distribution of trace metal levels in the general healthy population showed lower values. The results in this study can provide background data for clinical studies associated with trace metal exposure in the korean population.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.5
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• pp.920-927
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• 1998

The purpose of this study was to investigate the correlation between serum component and immune function in Korean female college students according to the body mass index. There were significant increased in weight, body fat, LBM, TBW, WHR in subjects as their BMI increase(p<0.001). It was found obese group had higher levels of serum components such as total cholesterol, LDL-cholesterol and Cu levels than those of the normal group. There was a significant positive correlation between HDL-cholesterol level and NK cell number(p<0.01), between A/G ratio and T lympocytes number(p<0.05), between Cu levels and B lympocytes(p<0.01) in normal group. There was a significant positive correlation between TG level and CD4/8 ratio(p<0.001), between and IgG(p<0.001) in obese group.

• Nutritional Sciences
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• v.2 no.2
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• pp.88-92
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• 1999

The purpose of this study was to study the intake of heavy metals such af arsenic, lead and cobalt and the relationship of dietary heavy metals with serum iron, topper, and zinc, which play important roles in hematopoiesis, in healthy college women living in Choongnam Korea, where we have detected heavy metals (As, Pb, Co) in some marine products in previous studies. The nutritional status of the subjects (35 women) was evaluated by anthropometric measurements, 24-hr dietary recall for 3 days. And 3-day diets (by weighing method) and blood were collected to analyze As, Pb, Co, Fe, Cu, Zn, Hb, Hct, and MCHC. The mean age, height, weight, and BMI were 20 years, 158 cm, 55 kg and 22.42 kg/$m^2$, respectively. The mean daily energy intake was 85.85% of RDA for Koreans. The ratio of energy from carbohydrate, protein, and fat was 60 : 24 : 16. The mean daily intake of heavy metals (As, Pb, Co) was 1.77 mg/day, 75.21 $\mu$g/day and 21.12 $\mu$g/day. And the mean daily intake of iron, copper, and Zinc concentrations were 97, 68, and 92% of normal values. The mean serum heavy metals (As, Pb, Co) were 16.14 $\mu$g/dl, 4.32 $\mu\textrm{g}$/dl and 0.02 $\mu$g/dr, respectively Mean blood levels of Fe, Cu, Zn, Hb, Hct, and MCHC were at normal levels. Dietary heavy metals except Co were not significantly different from serum Fe, Cu, Zn and Hb, Hct, and MCHC. However, there was a tendency toward lower serum concentration of Fe, Hb, Hct, and MCHC in the subjects with higher heavy metals (As) intake. Among heavy metals, only dietary Co showed a significant negative correlation with Hb (p< 0.001) and Hct (p < 0.001).

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.5
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• pp.1124-1130
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• 1999

The purpose of this study was to investigate the effect of copper supplementation on mineral utilizations in rats. Intakes, excretions, and tissue levels of Fe, Zn, Cu, Ca, Mg, Na, K were compared in rats fed diet with 100, 200, 400% of copper requirement(1Cu, 2Cu and 4Cu, respectively) for 6 weeks. The feed intake was significantly higher in 2Cu compared to 1Cu group, however, body weight gain was not significantly different among the three groups. While the serum level of Fe was significantly decreased, that of Cu was significantly elevated by Cu supplementation. The liver and kidney levels of Mg and Na, intakes of all the minerals, urinary excretions of Fe, Cu, Ca and Mg were significantly higher in 2Cu and/or 4Cu compared to 1Cu. The fecal excretions, retentions and absorptions of all the minerals were not affected by Cu supple mentation. In summary, these results suggest that dietary Cu supplementation has no effect on mineral utilizations, but effect on distribution in tissues of rats.