• 대한미생물학회지
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• 제14권1호
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• pp.63-69
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• 1979

Antibodies to Propionibacterium acnes(Corynebacterium parvum) serotype I and serotype II in normal human sera were measured using a microtitre bacterial agglutination test. Of 168 sera tested, 53 sera(31.0%) exhibited higher agglutinin titres to serotype I than to serotype II and 34 sera(20.2%) gave higher titers to serotype II than to serotype I. Eighty-one sera(48.3%), however, showed similar antibody titres to both types. Antibodies to serotype I(x) and serotype II(y) showed high correlation(r=0.73, p<0.01) and regression equation was Y=1,078+0.73X. The mean antibody titre($log_2$) of 529 normal sera(male 447 and female 82) to serotype I was $5.49{\pm}1.36$, but there was no significant difference between male($5.45{\pm}1.36$) and female($5.74{\pm}1.36$). Bacterial agglutinin to Propionibacterium acnes in normal sera belonged to a 2-mercaptoethanol resistant IgG class.

• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.121-135
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• 1996

Actinomyces are Gram-positive, non-acid-fast, anaerobic or microaerophilic filamentous bacteria. These organisms are frequently detected from infected root canals and periapical lesion. The purpose of this study was to use indirect immunofluorescence to determine the prescence of select Actinomyces species in a survey of teeth associated with periapical lesion, to clarify the relationship between clinical symptoms of periapical lesions and the Actinomyces species and to study on the cross reaction among Actinomyces. Actinomyces israelii serotype I (ATCC 12102), Actinomyces israelii serotype II (ATCC 29322), Actinomyces viscosus serotype II (ATCC 19246), Actinomyces naslundii serotype I (ATCC 12104) were cultured in anaerobic condition. Rabbit antisera were prepared by intravenous injection of formalized whole cells. Indirect immunofluorescence method was used to achieve the purpose. The following results were obtained. 1. There was a relationship between Actinomyces and periapical disease. 2. A. israelii serotype I, II were frequently identified with Indirect Immunofluorescence and most often assosiated with periapical disease. In culture finding, there was no significant difference between each group. 3. Indirect Immunofluoresence is both more sensitive and more rapid than culture for identification of Actinomyces species in patients with periapical lesion. 4. A. israelii serotype I, II was highly isolated in infected root canals with local swelling, A. naslundii serotype I was highly isolated in those with foul odor, and A. israelii serotype I was found in higher frequncy in those with exudate than other bacteria. 5. In the Indirect Immunofluorescence (1 : 320), A positive cross reaction was obtained between A. israelii serotype I and A. israelii serotype II, also, A. viscosus serotype II and A. naslundii serotype I. There was no cross reaction between A. israelii serotype I, II and A. viscosus serotype II, A. naslundii serotype I.

• 대한미생물학회지
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• 제18권1호
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• pp.23-29
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• 1983

An epidemiological survey was carried out to identify the serotypes of Streplococcus mutans isolated from carious lesions of 65 caries-active subjects(CAS) and sound tooth surfaces of 40 caries-free subjects(CFS). The autoclaved antigen extract was performed on each isolate, and then, serotypes of the isolates were determined in agar-gel immunodiffusion test. The results were as follows: 1. S. mutans was found in 78% of the samples of CAS, and of CFS. The difference of isolation frequency between CAS and CFS was not observed. 2. Only one serotype per single subject was detected in 61% of total samples, in remaining 39% of samples two or more serotypes were detected. 3. In 41.2% of CAS samples plural serotypes of S. mutans were found, whereas 35.5% of CFS samples showed plural serotypes distribution. 4. The most frequently identified serotype in each subject was serotype c; 69.5% of subjects harbored serotype c S. mutans. Serotype d was next most frequently isolated from subjects, comprising 23.2%. 5. Serotype c strain was found in 64.7% of CAS, 77.4% of CFS. 6. Of the isolates from CAS and CFS, serotype c was most commonly found, comprising 48.8%, serotype d was found in 16.3%, serotypes f. e, and g comprising 13.2%, 9.3%, and 7.8% respectively. Serotypes a and b were also found but in far lower frequencies(2.3%, 0.8%). 7. Serotype c strains were more found in CFS than in CAS, but serotypes d and e were more found in CAS.

• 한국동물위생학회지
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• 제35권4호
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• pp.283-288
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• 2012

Streptococcus(S.) suis is a pathogen, causing meningitis, septicemia and sudden death in weaning piglets as well as fattening pigs. Using multiplex PCR method based S. suis capsular genes, 61 S. suis isolates was classified as serotypes 2, 7, 9 and untypable. Genotyping of S. suis isolates was analysed by PFGE pattern with treated Sma I restricted enzyme. Of the 61 S. suis, 25 (40.9%) were serotype 2, 6 (9.8%) were serotype 7, 5 (8.2%) were serotype 9, and 25 (40.9%) were untypable, respectively. Twenty four PFGE patterns were detected in this study and also PFGE patterns were classified according to serotype; serotype 2 was classified as 6 genotypes, serotype 7 was 5 genotypes, serotype 9 was 3 genotypes, and untypable was 11 genotypes, respectively.

• Restorative Dentistry and Endodontics
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• 제8권1호
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• pp.31-44
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• 1982

Streptococcus mutans strains were isolated from dental plaques of carious lesions of 53 patients on mitis-salivarius-bacitracin (MSB) and mitis-salivarius(MS) medium as a supplement. The epidemiological investigation was carried out to determine the biotypes and serotypes of S. mutans isolates. For the serotyping, autoclaved extract antigens from the isolates and serotype-specific antisera against seven known serotypes of S. mutans were prepared. The serotypes of the isolates were demonstrated in immunodiffusion test. In addtition, the prevalence of ${\beta}$-hemolysis on 5% sheep blood agar plate in restricted anaerobic condition and yellow pigment production on 5% sucrose agar plate in less anaerobic condition among the isolates were investigated. The results were as follows: 1. Forty-eight S. mutans strains were isolated from dental plaque samples of 33 patients (62.3%) among 53 patients. 2. Of the isolates, some strains were not grown on MSB medium. 3. Serotype c S. mutans was found in 60.6%, serotype d was found in 30.3% of the patients who were known to harbor S. mutans. 4. Of. the isolates, serotype c isolates were most prevalent (43.8%), serotype d isolates were 25.0%, and serotype b, e, f and g isolates were also found but in lower frequencies. Serotype a S. mutans were not detected. 5. The correlation between serotype and biotype of the isolates was found in 78.6% of the typing cases. 6. Strains revealed ${\beta}$-hemolysis were in 52.1% of the isolates, strains produced yellow pigment were in 47.9% of the isolates, and with one exception, all the strains were belong to serotype c, e and f. 7. The majority of the isolates which revealed ${\beta}$-hemolysis appeared to be yellow pigmented, these isolates were belong to serotype c, e and f.

• Journal of Microbiology
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• 제40권2호
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• pp.166-169
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• 2002

Twenty strains of Cryptococcus neoformams isolated from environmental and clinical sources in Korea were examined for their serotypes. Two environmental isolates from pigeon excreta belonged to C. neoformans var. neoformans serotypes A. Of the 18 isolates from clinical specimens, 17 belonged to C. neoformans vats, neoiomans (serotype A : 16, serotype D : 1) and one belonged to C. neoformans vats, gattii serotype B, which was culturally unusual, producing mucous colonies. This is the first report of the identification of C. neoformans roar, gattii serotype B from a patient in Korea.

• 대한미생물학회지
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• 제21권3호
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• pp.337-343
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• 1986

To clarify the existing serological varieties of Leptospira interrogans in Korea, 15 isolates of 1985 were serologically studied by cross agglutinin adsorption test. Fourteen cultures except HS 7 belonged to serogroup Icterohemorrhagiae. Isolate HM 3 and HV 8 were considered to belong to serotype mwogolo by their low residual homologous antibody(0.08 to 6.25%) after cross adsorption with serotype mwogolo referene culture and antiserum. Both residual homologous antibody of isolate HS 7 and serotype canicola reference culture remained 1.56%, accordingly HS 7 proved to belong to serotype canicola. The agglutinogens of isolate HY2, AP3, AP4, AP7 and AP9 are considered to closely related to serotype mwogolo, and HM4 and HS6 to serotype birkini, which remained to be further studied. The remaining 5 strains(HY1, HS5, HY10, 310-9, 310-19) could not be attached to any known serotype of Icterohemorrhagiae serogroup by cross adsorption test.

• 대한수의학회지
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• 제32권1호
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• pp.63-76
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• 1992

A study on the isolation of Yersinia from the feces of healthy pigs and the biotype and serotype and susceptibility to 16 antimicrobials was carried. Out of 853 pigs, Yersiniae were isolated from 349 pigs(40.9%). Of 349 isolates, 289 isolates(82.8%) were Yersinia enterocolitica and 54(15.5%) were Y kristensenii, 3(0.9%) were Y pesudotuberculosis and the rest 3(0.9%) were Y prederiksenii. Out of 289 isolates of Y enterocolitica, the predominants biotype was 3B comprising of 165 isolates(57.1%) and followed by biotype 2, comprising of 49 isolates(17.0%), bioptype 3A, comprising of 41 isolates(14.2%) and biotype 4 comprising of 23 isolates(8.0%). And the predominant serotype was 0 : 3 comprising of 231 isolates(79.9%) and followed by serotype 0 : 9 comprising of 42 isolates(14.5%) and 0 : 21 comprising of 10 isolates(3.5%). Y. enterocolitica were resistant to cephalothin(99%), novobiocin(99%), erythromycine(83%), ampicillin(83%) and carbenicillin(81%) and susceptible to amikacin(100%), colistin(100%), gentamicin(100%), kanamycin(100%), polymyxin B(100%), tobramycin(100%), chloramphenicol(99%), nalidixic acid(99%), neomycin(99%), streptomycin(99%) and tetracycline(99%). Most strains of biotype 2/serotype 0 : 9 were susceptible to carbenicillin(100%) and ampicillin(61%) but the other biotype/serotypes were resistant to these antibiotic.

• 대한소아치과학회지
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• 제38권1호
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• pp.9-16
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• 2011

최근 우리와 다른 민족 또는 다른 문화적 배경을 가진 사람들이 포함된 다문화가정이 증가하였고 그들에 대한 사회적 관심이 증가하고 있으나, 우리는 아직 이들의 구강상태에 대한 기초적인 자료도 없는 실정이다. 본 연구는 부모가 모두 한국인인 가정과 다문화 가정에서, 어머니와 어린이의 구강 상태를 검사하고, polymerase chain reaction(PCR)을 이용하여 치아우식증의 주요 원인균인 Streptococcus mutans의 serotype 분포와 전이에 대한 실험을 통해 다음과 같은 결과를 얻었다. 1) 한국인 가정에서는, serotype c는 95%, serotype e, f, k는 10%의 비율로 나타났으며, 다문화가정을 대상으로 하였을 때에는 serotype c는 68.8%, serotype e는 31.25%, serotype f는 43.75%, serotype k는 18.8%의 비율로 나타났다. 2) 어머니와 어린이 둘 다 serotype이 확인된 경우, 92.86%에서 어머니와 어린이가 하나 이상의 같은 serotype을 포함하는 것으로 나타났다. 3) 한 가지 serotype만 나타난 경우보다 두 종류 이상의 serotype이 확인된 경우에서 평균 S. mutans level이 높게 나왔으나 통계적으로 유의한 차이는 없었다(p>0.05). 4) 우식 위험이 높다고 볼 수 있는 S. mutans level 2를 기준으로 비교하였을 때, 각 그룹 간에 우식 위험도는 통계적으로 유의한 차이가 없었다(p>0.05). 5) 그룹에 따른 serotype의 수, 우식 이환율, S. mutans level 간에는 유의한 상관관계가 없었다(p>0.05).

• 대한미생물학회지
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• 제17권1호
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• pp.57-66
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• 1982

The correlation between the serotypes and sensitivity distribution of Pseudomonas aeruginosa was studied with stock strains of department, strains isolated from various clinical sources and strains of haspital environment of the past two years. Two hundred and fifty seven strains were typable and 38 strains were untypable out of 295 clinical sorces. Serotype B was most provalent(27.6%) followed by serotype G(24.9%), serotype E(23.3%) and serotype A(7.8%) among 257 typable strains. However, no serotype J,L and M were observed. Serotype B,G,E and A were isolated from pus, sputum, wound, burn site and urine. Serotypes E were most frequently isolated from nasal discharge and serotypes K were isolated from pus. There were no apparent differences in sensitivity distribution of streptomycin, carbenicillin and ampicillin between the clinical and The environmental origin. However, the strains of environmental origin were found to be relatively more susceptable to tetracycline, gentamicin and fradiomycin than the strains of clinical sources in high concentration. The strains of clinical sources of serotype B,G,E and A showed different resistant patterns to all antimicrobial agents except for carbenicillin. Serotype E showed the highest percentage resistance followed by serotype G,B and A.