• 생명과학회지
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• 제10권2호
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• pp.157-163
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• 2000

One hundred eight strains of Pseudomonas aeruginosa isolated from the patients (sputum, urine, burn skin, stool and blood) of Pusan National University hospital were tested for exonezyme production, antimicrobial susceptibility and serotyping. The results obtained were as follow: In exonezyme production test, 50 strains (46.30%) produced both protease and elastase. Thirty three strains (30.55%) did not produce any exoenzyme, 18 strains (16.67%) produced only protease and 7(6.48%) stains only produced elastase. As the result of antimicrobial susceptibility by the disc diffusion method, most strains were resistant to sulfamethoxazole (96.30%). But the resistant rate against gentamicin and ticarcillin were 47.23% and 46.30% respectively. The resistant rate to other antibiotics were less than 40%. All strains could be serologically typed. Most strains were identified as type Ⅲ: among them, 51 strains were belonged to serotype E. The correlation of serotype and exoenzyme production was not found.

• 한국동물위생학회지
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• 제25권3호
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• pp.237-243
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• 2002

The study was performed to investigate the antibody distributions of 4 swine respiratory disease including M hyopneumoniae, P multocida, A pleuropneumoniae serotype 2 and 5 in Daegu area by enzyme-linked immunosorbent assay(ELISA). 1. The overall sero-positive rates were 55.6% in June, 48.0% in August, 51.3% in October and 25.4% in November. 2. The positive reaction rates to M hyopneumoniae, P multocida, A pleuropneumoniae serotype 2 and 5 were found to be 50.0%, 36.5%, 55.0%, and 42.0% respectively. 3. The antibody titers were distributed on range 20～80 in M hyopneumoniae, 20～80 in P multocida, 160～640, 20～80 in A pleuropneumoniae serotype 2 and 5.

• 한국미생물·생명공학회지
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• 제51권3호
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• pp.314-316
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• 2023

Colistin is one of the last-resort antibiotics used to treat multidrug-resistant Gram-negative bacterial infection in both human and animals. Here, we report the complete genome sequence of colistin-resistant Salmonella enterica serotype Enteritidis strain CRSE-01 isolated from poultry carcass in South Korea. The assembled genome consists of a 4,783,907-bp circular chromosome containing numerous antimicrobial resistance genes and a 59,372-bp plasmid.

• 한국환경보건학회지
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• 제23권4호
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• pp.45-49
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• 1997

Clostridium perfringens A형이 생산하는 장독소를 검색해본 결과, RPLA법에 있어서는 2배로 희석한 용액으로부터 64배로 희석한 용액 (NCTC 8239 Hobbs serotype 3 CPE$^+$)에서까지 양성반응을 보였으며 PCR 기법에 있어서는 10 pg 희석 용액까지 364 bp의 장독소 DNA fragment(NCTC 8238 Hobbs serotype 2 CPE$^+$)를 확인 할수 있었다. 그러므로 장독소를 검색하기 위해서는 PCR기법이 RPLA법에 비하여 훨씬 감도가 높음을 확인할 수 있었다.

• Clinical and Experimental Pediatrics
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• 제52권4호
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• pp.508-511
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• 2009

폐구균(Streptococcus pneumoniae)은 소아, 특히 어린 영아에서 심한 침습성 질병을 일으키는 중요한 원인균이다. 2003년부터 우리나라에서 접종하기 시작한 7가 폐구균백신(PCV7)은 소아에서 침습성 폐구균성 폐렴과 수막염을 예방하는 것으로 알려져 왔다. 그러나 최근 7가 폐구균백신을 접종한 이후부터 비7가 폐구균 혈청형(non-PCV7 serotype) 감염 발생률이 증가하고 있다. 14개월 된 여아가 내원 3일 전부터 열과 구토가 있었으나 증상 치료만 하고 있던 중 갑작스런 의식불명과 기면(lethargy)으로 응급실로 내원하였다. 입원 후 즉각적인 진단과 치료를 시행했음에도 불구하고 환자는 빠르게 혼수와 뇌사 상태로 진행하였다. 환자는 이전에 3회의 7가 폐구균백신을 접종하였으나 최종 진단은 비7가 폐구균 혈청형인 폐구균 19A 수막염이었고, 입원 20일째 사망하였다. 이는 한국에서 문헌상 보고된 적이 없는, 폐구균 19A 혈청형 수막염으로 사망한 첫번째 증례이므로 이에 보고하는 바이다.

• 한국어병학회지
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• 제1권1호
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• pp.45-50
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• 1988

1985년(年) 7월(月)부터 1986년(年) 12월사이에 양식(養殖)방어, 참돔 및 돌돔으로 부터 분리(分離)한 26균주(菌株)의 Vibrio anguillarum에 대한 혈청학적(血淸學的) 동정(同定)의 실시(實施)하였다. 이 분리균(分離菌)들을 Kitao et al. (1983)이 보고(報告)한 6가지 혈청형(血淸型)의 V. anguillarum에 대하여 내열성(耐熱性) O항원(抗原)을 이용(利用)하여 상호응집반응(相互凝集反應)시켜본 결과(結果), 1. 본(本) 분리균(分離菌)은 모두 하나의 동일(同一)한 혈청형(血淸型)을 가지고 있었으며 Kitao et al. (1983)이 보고(報告)한 혈청형(血淸型)C(대표균주(代表菌株) PT-213)와 만이 응집반응(凝集反應)하였다. 2. 본(本) 분리균(分離菌)은 모두 PT-213혈청(血淸)에 대하여 동일(同一)한 2중(重)의 침강선(沈降線)을 형성(形成)하였다. 3. 본(本) 분리균(分離菌)은 모두 PT-213의 인자항혈청(因子抗血淸)에 대하여만 특이반응(特異反應)을 하였다. 따라서 본(本) 분리균(分離菌)들의 혈청형(血淸型)은 모두 Kitao et al.(1983) 이 보고(報告)한 혈청형(血淸型)C와 동일한 것으로 판단되었다.

• 대한수의학회지
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• 제34권3호
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• pp.511-516
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• 1994

이 실험은 도축돈의 폐에서 Pasteurella multocida 감염의 발생빈도를 조사하고 분리된 Pasteurella multocida의 항생제에 대한 약제감수성, 생화학적특성, 협막혈청형의 분류 및 독소생성능에 대해서 조사하였다. 실험재료로는 영남지방의 돼지 450두가 사용되었으며, 재료채취는 1992년 4월부터 1993년 3월 사이에 시행되었다. P multocida는 도축돈의 폐 450예에서 80주가 분리되어 17.7%의 분리율을 나타내었다. 분리균 대부분의 생화학적 및 배양성상은 reference strain의 것과 일치하였으며, 분리균 80주의 협막혈청형을 동정한 결과 77주가 type A, 나머지 3주가 type D로 나타나 각각 96.3%, 3.8%의 분포비를 나타내었다. 항생제에 대한 감수성검사에서 모든 분리균주는 ampicillin, ceftiofur, cephalothin, ciprofloxacin, penicillin-G 등에 대해서는 매우 감수성이 높았으며 이들 분리균중 일부는 sulfadimethoxine과 streptomycin에 내성을 나타내었다. 분리균 80주중 61주가 독소생성능이 있는 것으로 나타났으며, type A 77, type D 3주중 76.6%가 각각 독소생성능이 있는 것으로 나타나 협막혈청형간에 독소생성능의 뚜렷한 차이가 인정되지 않았다.

• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.789-797
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• 2010

The limited information on differential gene expression in the different serotypes of Actinobacillus pleuropneumoniae has significantly hampered the research on the pathogenic mechanisms of this organism and the development of multivalent vaccines against A. pleuropneumoniae infection. To compare the gene expressions in the A. pleuropneumoniae strains CVCC259 (serotype 1) and CVCC261 (serotype 3), we screened the differentially expressed genes in the two strains by performing representational difference analysis (RDA). Northern blot analyses were used to confirm the results of RDA. We identified 22 differentially expressed genes in the CVCC259 strain and 20 differentially expressed genes in the CVCC261 strain, and these genes were classified into 11 groups: (1) genes encoding APX toxins; (2) genes encoding transferrin-binding protein; (3) genes involved in lipopolysaccharide (LPS) biosynthesis; (4) genes encoding autotransporter adhesin; (5) genes involved in metabolism; (6) genes involved in the ATP-binding cassette (ABC) transporter system; (7) genes encoding molecular chaperones; (8) genes involved in bacterial transcription and nucleic acid metabolism; (9) a gene encoding protease; (10) genes encoding lipoprotein/membrane protein; and (11) genes encoding various hypothetical proteins. This is the first report on the systematic application of RDA for the analysis of differential gene expression in A. pleuropneumoniae serotypes 1 and 3. The determination of these differentially expressed genes will serve as an indicator for future research on the pathogenic mechanisms of A. pleuropneumoniae and the development of a multivalent vaccine against A. pleuropneumoniae infection.

• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.369-377
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• 1998

Rotavirus is a major cause of severe gastroenteritis in young children and animals throughout the world. The VP7 of rotavirus is thought to induce the synthesis of neutralizing antibodies and to be responsible for determining viral serotypes. The cDNA coding for the VP7 capsid protein of human rotavirus, obtained from Korean patients (HRV-Y14), was cloned and its nucleotide sequence was determined. Comparative analysis of the nucleotide sequences between VP7 of Y14 and that of other foreign isolates showed $92.7~95.2\%$ homology to G1 serotypes (RV-4, KU, K8, WA), $74.2\%$ homolgy to G2 serotype HU-5, $76.4\%$ homology to G3 serotype SA-11, and $77.6\%$ homology to G4 serotype A01321. These data suggest that HRV-Y14 can be classified as a G1 serotype. cDNA coding for VP7 of HRV-YI4 was subcloned into the baculovirus vector and the VP7 glycoprotein was expressed in insect cells. The expressed proteins in Sf9 cell extract and tissue culture fluid were separated on SDS-PAGE, and Western blot analysis with monoclonal antibody raised against the synthetic peptide containing 21 amino acids within the VP7 conserved region was performed. The molecular weight of recombinant VP7 was estimated to be 36 kDa which is about the same size as the native VP7. Addition of tunicamycin in the culture media caused a reduction of the molecular weight of the recombinant VP7 indicating that the expressed protein was glycosylated.

• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.487-496
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• 1995

Previous studies have demonstrated that not all A. actinomycetemcomitans produced significant level of leukotoxic factor and its leukotoxicity have associated with serotype and genetic variation. Our aim was to investigate on the interrelationship between serotype and leukotoxicity of an A. actinomycetemcomitans consisting of 13 clinically well characterized. Korean isolates and to evaluate if particular virulent clonal types of A. actinomycetemcomitans are associated with periodontal disease. For this study, 13 strains of A. actinomycetemcomitans from 6 patients with periodontal disease were isolated and identified by using a selective medium(tryptic soy agar supplemented with 10% serum, $75{\mu}g$ of bacitracin and $5{\mu}g$ of vancomycin per ml) in 10% C02 incubator for 3days with routine Gram staining, colony morphology and biochemical test..For serotyping, antisera were prepared from reference strains of 5 serotypes. (ATCC 29523,Y4, SUNY aB 67, IDH 781, IDH 1705) and then ammonium sulfate precipitation, immunoabsorption and indirect immunofluoroscent procedures were done. For analysis of leukotoxicity, sonic extract of A. actinomycetemcomitans exposed to PMN, and trypan blue was stained for counting the cell viability. Finally Southern blot analyses of genomic DNA digested with the restriction enzyme Tag I was done and the Southern blots were hybridized with the 530bp fragment, termed delta 530, originating from the ltx promoter of strain 652 and deleted from strain JP2. Also ltxA-3.1 and SC2 probe from strain JP2 were hybridized with genomic DNA fragments. Results reveal that strains isolated showed approximately equal proportions of 3 serotypes(b, d, e) and serotype b was not detected. 2 patients harbored 2 different serotypes in the same disease site. The prevalence of leukotoxic strain was 23% and there was no relationship between serotype, leukotoxicity and clinical observations. Especially virulent clonal types of Actinobacillus actinomycetemcomitan (JP2 strain) could not found. Further studies are necessary on the genetic polymorphism of leukotoxin and its relations to clinical status.