• Journal of dental hygiene science
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• v.12 no.2
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• pp.109-113
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• 2012

One hundred seventy four Streptococcus pneumoniae clinical isolates were categorized depending on the types of specimens, the age and the gender, respectively. All isolates were analyzed the characteristics of the multi-drug resistance including levofloxacin antibiotics. In the results of analysis depending on the type of samples, it had been confirmed that sputum was the main source of pneumonia infection because 156 of 174 strains (89.7%) were isolated in sputum samples. The opportunity for isolating the S. pneumoniae that had tolerance to levofloxacin was increased in over 51 age patients group compared with other age and male group. Eight strains of isolates were evaluated higher resistant to levofloxacin, and those also showed multi-drug resistant including penicillin, tetracycline, erythromycin, clindamycin and trimethoprim-sulfamethoxazole. In the results of sequence analysis of quinolone resistance determining region in SP32 (MIC $64{\mu}g/mL$) and SP96 (MIC $8{\mu}g/mL$) which were levofloxacin resistant strains, an amino acid substitutions were found Ser-81$\rightarrow$Phe in both GyrA of SP32 and SP96, and Ser-11$\rightarrow$Gly in only SP96. A Ser-79$\rightarrow$Phe substitution of ParC was found in both.

• BMB Reports
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• v.32 no.1
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• pp.20-24
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• 1999

The first step of the branch pathway in tryptophan biosynthesis is catalyzed by anthranilate synthase, which is subjected to feedback inhibition by the end product of the pathway. The $trpE^{FBR}$ gene from a mutant Escherichia coli strain coding for anthranilate synthase that was insensitive to feedback inhibition by tryptophan has been cloned. To identify the amino acid changes involved in the feedback regulation of anthranilate synthase, the nucleotide sequence of the mutant $trpE^{FBR}$ gene was determined. Sequence analysis of the $trpE^{FBR}$ gene revealed that four bases were changed in the structural gene while alteration was not found in the 5' control region. Among these base changes, only two base substitutions caused the alterations in amino acid sequences. From the results of restriction fragment exchange mapping, the 61st nucleotide, C to A substitution, that changed $Pro^{21}{\rightarrow}Ser$ was identified as the cause of the desensitization to feedback inhibition by tryptophan. Additional feedback-resistant enzymes of the E. coli anthranilate synthases were constructed by site-directed mutagenesis to examine the effect of the $Ser^{40}\;{\rightarrow}\;Arg^{40}$ change found in the $trpE^{FBR}$ gene of Brevibacterium lactofermentum. From the feedback inhibition analysis, the $Pro^{21}{\rightarrow}Ser$ and $Ser^{40}{\rightarrow}Arg$ mutants maintained about 50% and 90% of their maximal activities, respectively, even at the extreme concentration of 10 mM tryptophan. From these results, we suggest that the $Pro^{21}$ and $Ser^{40}$ residues are involved in the tryptophan binding in the E. coli enzyme.

• Journal of Life Science
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• v.20 no.10
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• pp.1544-1548
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• 2010

Moxifloxacin (MF) - resistant mutants of Mycoplasma hominis (M. hominis) were generated by stepwise selection in increasing concentrations of MF, and six strains of MF resistant M. hominis mutants - M1, M4, M8, M16, M32, and M64 - in which MICs of MF were 0.5, 4, 8, 16, 32, 64 ${\mu}g$/ml, respectively, were generated. Compared to the sequence of M. hominis PG21, all mutants harbored amino acid substitutions of Arg-163 Thr in GyrA, and Pro-445 Gln in ParE. While the concentrations were getting higher, an additional amino acid substitution was found at Ser-153 Lys in GyrA (${\geq}4{\mu}g/ml$), Ser-91 Ile in ParC (${\geq}16{\mu}g/ml$), and Val-450 Phe (${\geq}64{\mu}g/ml$) in GyrB. These substitutions seem to have an impact on resistance to MF, and GyrB change was found only in the highest concentration and seems to be associated with high-level resistance to MF. This, as far as we know, is the first description of a relationship between MF resistance phenotype and genotype.

• Microbiology and Biotechnology Letters
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• v.26 no.6
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• pp.507-514
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• 1998

The strain K-54, the best producer of fibrinolytic enzyme, was isolated from Korean traditional food Chung Guk Jang and identified as Bacillus subtilis. Fibrinolytic enzyme was purified and characterized, and its molecular weight was determined. The fibrinolytic enzyme activity was increased about 66.9 times via purification with recovery yield of 10.1%. The optimum pH and temperature of this enzyme were 11 and $65^{\circ}C$. The enzyme was stable within a pH range 8-12 and unstable at 9$0^{\circ}C$. The molecular weight was estimated to be 29,000 dalton in the form of monomer with no other subunit. The isoelectric point was calculated 8.67. N-terminal sequence was identified Ala-Gly-Ser-Val-Pro-Try-Gly-Ser. Km value of the enzyme for $\alpha$-casein was calculated to be 0.31 (3.1 mg/$m\ell$). The enzyme activity highly inhibited by PMSF at 1 nM.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.7
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• pp.685-691
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• 2003

This paper newly analyzes the effect of the phase noise in the frequency synthesizer on the performance of SFH/M-NCFSK system by standard frequency deviation(equation omitted) when noncoherent FSK demodulation of the square-law detector is considered. We derive the SER in the SFH system and analyze the effect of phase noise on the SFH/M-NCFSK system performance according to the hopping frequency spacing (1/T$\_$h/) and the variation of the standard frequency deviation (equation omitted). The required SNR is about 13.4 dB to meet Ps=10$\^$-3/ when the standard frequency deviation is about 4.0 Hz and the hopping frequency spacing (1/T$\_$h/) in the SFH/2-NCFSK system is 30. So, there is about 2.4 dB power penalty than the phase noise-free system. If the hopping frequency spacing 1/T$\_$h/ is under 30, the error floor may happen and SER considerably grows up. We show that the analytic results closely match with the simulation results.

• BMB Reports
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• v.33 no.6
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• pp.531-536
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• 2000

Tropomyosin (TM) is an important actin binding protein involved in regulation of muscle contraction. Unacetylated striated tropomyosin failed to bind to actin whereas unacetylated smooth tropomyosin bound well to actin. It has been demonstrated that high actin affinity of unacetylated ${\alpha}-tropomyosin$ was ascribed to the carboxyl terminal amino acid residues. In order to define the role of the carboxyl terminal residues of tropomyosin molecule on actin binding, two mutant tropomyosins were constructed. TM11 is identical to the striated tropomyosin except that the carboxyl terminal last three amino acids was replaced with $^{282}NNM^{284}$ whereas in TM14 $^{276}HA^{277}$ was substituted with smooth specific $^{276}QT^{277}$. TM11 and TM14 were overproduced in Escherichia coli and analyzed for actin affinity. The apparent binding constants (Kapp) of unacetylated tropomyosins were $2.2{\times}10^6M^{-1}$ for sm9, $1.03{\times}10^6M^{-1}$ for TM14, $0.19{\times}10^6M^{-1}$ for TM11, $>0.1{\times}10^6M^{-1}$ for striated, respectively. This result indicated that higher actin affinity of the unacetylated smooth tropomyosin was primarily attributed to the presence of QT residues in the smooth sequence. In case of the Ala-Ser (AS) dipeptide extension of the amino terminus of tropomyosin, Kapp were $21.1{\times}10^6M^{-1}$ for AS-sm9, $8.0{\times}10^6M^{-1}$ for AS-11, $4.7{\times}10^6M^{-1}$ for AS-14, $3.8{\times}10^6M^{-1}$ for AS-striated. AS-TM11 showed considerably higher actin affinity than AS-TM14, implying that interaction of Ala-Ser of the amino terminus with the carboxyl terminal residues. Since Kapp of AS-TM11 was significantly lower than that of AS-sm9, the presence of QT might be required for restoration of high actin affinity of the smooth ${\alpha}-tropomyosin$. These results suggested that the carboxyl terminal amino acid residues Glutamine275-Threonine276 are important for actin affinity of the recombinant smooth ${\alpha}-tropomyosin$, particularly of unacetylated smooth ${\alpha}-tropomyosin$.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.11C
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• pp.1045-1052
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• 2005

The symbol error probability of M-ary PSK (MPSK) and QAM (MQAM) systems using the branch with the largest signal-to-noise ratio (SNR) at the output of L-branch selection combining (SC) in frequency-nonselective slow Nakagami fading channels with an additive white Gaussian noise (AWGN) is derived theoretically For integer values of the Nakagami fading parameter m, the general formula for evaluating symbol error rate (SER) of MPSK signals in the independent branch diversity system comprises numerical analyses with the integral-form expressions. An exact closed-form SER performance of MQAM signals under the effect of SC diversity via numerical integration is presented. These performance evaluations allow designers to determine M-ary modulation methods for Nakagami fading channels.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.20 no.1
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• pp.95-100
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• 2020

This paper related with the performance evaluation of mSE-MMA (modified Signed Error-Multi Modulus Algorithm) adaptive equalization algorithm which is possible to reduce the distortion that is occurs in nonlinear communication channel like as additive noise, intersymbol interference and fading. The SE-MMA algorithm are emerged in order to reducing the computational load compared to the presently MMA algorithm, it has the degraded equalization performance by this. In order to improve the performance degradation of SE-MMA, the mSE-MMA controls the step size according to the existence of arbitrary radius circle of equalizer output is centered at transmitted symbol point. The performance of proposed mSE-MMA algorithm were compared to present SE-MMA using the same channel and noise environment by computer simulation. For this, the recoverd signal constellation which is the output of equalizer, residual isi and MD (Maximum Distortion), MSE learning curve which is represents the convergence performance and SER which is represents the roburstness of noise were used as performance index. As a result of simulation, the mSE-MMA has more superior to the SE-MMA in every performance index, and was confirmed that mSE-MMA has roburstness to the noise in the SER performance than SE-MMA especially.

• Proceedings of the Korea Information Processing Society Conference
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• 2023.11a
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• pp.526-527
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• 2023

Speech emotion recognition(SER) is one of the interesting topics in the machine learning field. By developing multi-modal speech emotion recognition system, we can get numerous benefits. This paper explain about fusing BERT as the text recognizer and CNN as the speech recognizer to built a multi-modal SER system.

• Journal of The Korean Astronomical Society
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• v.48 no.1
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• pp.1-7
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• 2015

The orbital period changes of the W UMa eclipsing binary AU Ser are studied using the (O-C) method. We conclude that the period variation is due to mass transfer from the primary star to the secondary one at a very low and decreasing rate $dP/dt=-8.872{\times}10^{-8}$, superimposed on the sinusoidal variation due to a third body orbiting the binary with period $42.87{\pm}3.16$ years, orbital eccentricity $e=0.52{\pm}0.12$ and a longitude of periastron passage ${\omega}=133^{\circ}.7{\pm}15$. On studying the magnetic activity, we have concluded that the Applegate mechanism failed to describe the cycling variation of the (O-C) diagram of AU Ser.