• 한국국방경영분석학회지
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• 제36권1호
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• pp.15-27
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• 2010

현대전에 있어서 대량의 화력, 신속한 기동력은 전승의 핵심요소이기 때문에 전쟁에서 승리하기 위해서는 이에 대한 과학적인 운용이 요구된다. 따라서 첨단기술을 이용한 치밀한 화력계획과 신속 정확한 타격으로 적에게 치명적인 피해를 줄 수 있어야 한다. 특히 사격이 작전개시 전에 미리 계획하여 수행하게 되는 경우는 표적별 사격 필요량에 의해 사격부대의 사격량을 할당함과 동시에 사격순서를 결정한 후 전체사격시간을 최소화함으로써 사격의 효과를 높일 수 있다. 따라서 본 연구에서는 이러한 사격완료시간을 최소화하기 위하여 동시에 표적을 타격하는 경우와 현재 야전부대에서 운용하고 있는 개별적으로 사격하는 문제 그리고 사격부대를 혼합하여 사격하는 경우의 최적의 사격완료시간을 비교하고, 사격시간 최소화를 위해 각 표적을 어떻게 할당할 것인지 수리모형을 이용하여 방안을 제시함으로써 작전계획에 맞는 사격계획표를 작성하는 것이다.

• 통상정보연구
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• 제9권2호
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• pp.19-40
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• 2007

In recent, T-commerce is widely dispersed as alternative type of commerce. It is forecasted that t-commerce system is used more than e-commerce system. Therefore more and more t-commerce-related industries are also recognizing that t-commerce is a critical business model. It is needed to understand the concept of t-commerce and develop the t-commerce marketing strategy. CEO analyses consumer's behaviors according to the data about buyers and applies the advantage of t-commerce to the communication with customers. This t-commerce system plays an important role in maximizing customer satisfaction and affecting their intention to reuse it. Therefore this paper attempts to identify T-commerce critical success factors and divide between use-intention group and unuse-intention group by taking out a discriminant function by the discriminant analysis. This lays a foundation in developing T-commerce strategy. According to the discriminant function extracted, convenience factor, amusement factor, system quality factor, product perception factor are significant in the sequence of influential degree. However, usefulness factor and speedy connection factor are not significant. In result, the target hitting rate is 77.9% in the first unuse-intention group and it is 95.2% in the second use-intention group. The total discriminant target hitting rate is computed to higher value, 86.55%. The statistic package, SPSS 12.0, is used to survey and analyse data and test the hypothesis. The validity and reliability of variables are verified by both reliability analysis and factor analysis. The discriminant analysis is used to tell the difference between use-intention group and unuse-intention group.

• 한국산학기술학회논문지
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• 제16권4호
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• pp.2757-2763
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• 2015

p-Snake는 기존의 동적윤곽모델(Active Contour Model)에 원형에너지를 추가로 적용한 에너지 최소화 알고리즘으로 에지 정보가 명확하지 않은 영역에서의 윤곽선 추출을 위해 사용된 방법이다. 본 논문에서는 원과 직선 프리미티브(primitive)의 조합으로 표현되는 가변 원형(prototype)과 퍼지 함수를 적용한 원형에너지장의 생성 기법을 제안하여 p-Snake의 윤곽선 추출 성능을 개선하였다. 제안 방법은 입력된 부품 코드를 기반으로 원형을 정의하고 전처리 과정을 통해 구해진 각 프리미티브 구간에서 대략적인 초기 윤곽을 검출한 후, 프리미티브들이 가변적으로 적응하여 원형을 생성하고 여기에 원형과의 거리에 따른 윤곽 확률을 퍼지 함수를 통해 계산하여 원형에너지 장을 생성하였다. 이를 p-Snake에 적용하여 다양한 소형부품들을 대상으로 준비한 200장의 영상에서 윤곽선을 검출하고, 원형과의 유사도를 비교한 결과 적응 원형을 사용한 p-Snake가 기존의 Snake에 비해 약 4.6% 가량 우수함을 보였다.

• Annals of Clinical Neurophysiology
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• 제2권2호
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• pp.119-124
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• 2000

Background : We studied EEG changes during pilocarpine-induced status epilepticus(SE), a widely used model whose EEG characteristics have not been fully described previously. Methods : Male Sprague-Dawley rats weighing 250-350 grams were used as subjects. SE was induced 5-7 days after placement of chronic epidural electrodes, using 360-380 mg/Kg pilocarpine IP. Rats were observed with continuous EEG recording following pilocarpine injection until end of the SE episode. Results : SE occurred in 10/12 rats studied. SE began with a series of discrete seizures $11.1{\pm}3.93$ minutes after pilocarpine injection. $5.2{\pm}2.71$ seizures occurred over $10.9{\pm}4.62$ minutes, until the EEG converted to a waxing and waning pattern, during which the amplitude and frequency of epileptiform activity increased. After $1.4{\pm}1.82$ minutes, a pattern of continuous high amplitude rapid spiking was established. Continuous spiking continued for $3.4{\pm}0.48$ hours with a very gradual decline in amplitude and frequency, until periodic epileptiform discharges(PEDs) began to occur. The EEG consisted primarily of PEDs for another $7.4{\pm}3.09$ hours, until electrographic generalized seizures began to occur. These continued for $5.8{\pm}4.82$ hours until death. Duration of SE was $17.0{\pm}5.88$ hours. Flat periods were a prominent feature during all EEG patterns in this model. Conclusion : EEG features distinctive in pilocarpine SE(but not unique to it) include flat periods during all patterns and resumption of continuous spiking episodes after the onset of PEDs. The sequence of discrete seizures to waxing and waning to continuous spiking to PEDs was identical to that which has been described in humans and other animal models.

• 천문학논총
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• 제30권2호
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• pp.201-204
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• 2015

The common-envelope process is a complicated phase in binary evolution. A lot of effort has been dedicated to study the common-envelope stage, but many questions related to this process are yet to be answered. If one member of the binary survives the common-envelope phase, the binary will emerge as a white dwarf accompanied by a low-mass main sequence star in close orbit, often referred as a post common-envelope binary (PCEB). SDSS J0745+2631 is among the list of newly found PCEBs from the Sloan Digital Sky Survey (SDSS). This star is proposed to be a strong eclipsing system candidate due to the ellipsoidal modulation in its light curve. In this work, we aim to confirm the eclipsing nature of SDSS J0745+2631 and to determine the stellar and orbital parameters using the software Binary Maker 3.0 (BM3.0). We detected the primary eclipse in the light curve of SDSS J0745+2631 in our follow-up observation from January 2014 using the ULTRASPEC instrument at the Thai National Observatory. The data obtained on 7th and 8th January 2014 in g filter show an evident drop in brightness during the eclipse of the white dwarf, but this eclipse is less prominent in the data taken on the next night using a clear filter. According to our preliminary model, we find that SDSS J0745+2631 hosts a rather hot white dwarf with an effective temperature of 11500K. The companion star is a red dwarf star with a temperature of 3800K and radius of 0.3100 $R_{\odot}$. The red dwarf star almost fills its Roche lobe, causing a large ellipsoidal modulation. The mass ratio of the binary given by the Binary Maker 3.0 (BM3.0) model is M2/M1 = 0.33.

• Journal of Biosystems Engineering
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• 제38권1호
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• pp.1-8
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• 2013

Purpose: Path planning and tracking algorithms applicable to various agricultural operations, such as tillage, planting, and spraying, are needed to generate steering angles for auto-guidance tractors to track a point ahead on the path. An optimal coverage path algorithm can enable a vehicle to effectively travel across a field by following a sequence of parallel paths with fixed spacing. This study proposes a path generation and tracking algorithm for an auto-guided Korean tractor with a tillage implement that generates a path with C-type turns and follows the generated path in a paddy field. A mathematical model was developed to generate a waypoint path for a tractor in a field. This waypoint path generation model was based on minimum tractor turning radius, waypoint intervals and LBOs (Limit of Boundary Offsets). At each location, the steering angle was calculated by comparing the waypoint angle and heading angle of the tractor. A path following program was developed with Labview-CVI to automatically read the waypoints and generate steering angles for the tractor to proceed to the next waypoint. A feasibility test of the developed program for real-time path tracking was performed with a mobile platform traveling on flat ground. The test results showed that the developed algorithm generated the desired path and steering angles with acceptable accuracy.

• 미생물학회지
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• 제41권4호
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• pp.239-245
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• 2005

Transformation-associated recombination (TAR) 클로닝 법은 복잡한 게놈으로부터 염색체 내의 특정부위나 유전자를 선택적으로 분리할 수 있다. 이 방법은 목적 유전자에 근접한 작은 게놈DNA 염기서열 정보를 필요로 한다. 이 기술은 효모의 spheroplast transformation을 시키는 동안 목적으로 하는 유전자의 5' 또는 3' 서열을 포함하고 있는 TAR vector와 게놈DNA사이에서 일어나는 상동성재조합에 의해 이루어진다. 본 연구에서는 plasmid 모델시스템을 이용하여 target hooks 내에 존재하는 비상동성 염기서 열이 상동성재조합에 미치는 영향을 조사하였다. plasmid에 존재하는HIS3유전자와 변형시킨 his3-TRP1-his3 단편 사이의 상동성재조합의 효율은 $Ura^+$ 형질전환체의 형질분석에 의해 이루어졌다. $Ura^+$ 형질전환체의 수는 7종류의 서로 달리 변형된 his3-TRP1-his3 단편들을 사용하였을 매 거의 동일하게 나타났다. 그러나 $Trp^+His^+$ positive recombinants의 빈도는 변형된 his3-TRP1-his3 단편 내에 비상동성 영역에 부정확한 간격을 지닐 때 현저한 감소를 나타내었다. 이러한 결과로서, 부정확한 간격이 target hook과 substrate DNA 사이에 일어나는 상동성재조합을 방해하는 것으로 사료된다. 그러므로 이종간의 상동유전자를 클로닝 할 때에는 target hook내의 비상동성 염기서열이 존재한다면 이것이 정확한 간격을 지니는지 여부를 중요란 요인으로 고려해야 한다.

• 생물환경조절학회지
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• 제31권3호
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• pp.152-162
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• 2022

본 연구는 데이터를 기반으로 한 인공지능 기계학습 기법을 활용하여 온실 내부온도 예측 시뮬레이션 모델을 개발을 수행하였다. 온실 시스템의 내부온도 예측을 위해서 다양한 방법이 연구됐지만, 가외 변인으로 인하여 기존 시뮬레이션 분석방법은 낮은 정밀도의 문제점을 지니고 있다. 이러한 한계점을 극복하기 위하여 최근 개발되고 있는 데이터 기반의 기계학습을 활용하여 온실 내부온도 예측 모델 개발을 수행하였다. 기계학습모델은 데이터 수집, 특성 분석, 학습을 통하여 개발되며 매개변수와 학습방법에 따라 모델의 정확도가 크게 변화된다. 따라서 데이터 특성에 따른 최적의 모델 도출방법이 필요하다. 모델 개발 결과 숨은층 증가에 따라 모델 정확도가 상승하였으며 최종적으로 GRU 알고리즘과 숨은층6에서 r² 0.9848과 RMSE 0.5857℃로 최적 모델이 도출되었다. 본 연구를 통하여 온실 외부 데이터를 활용하여 온실 내부온도 예측 모델 개발이 가능함을 검증하였으며, 추후 다양한 온실데이터에 적용 및 비교분석이 수행되어야 한다. 이후 한 단계 더 나아가 기계학습모델 예측(predicted) 결과를 예보(forecasting)단계로 개선하기 위해서 데이터 시간 길이(sequence length)에 따른 특성 분석 및 계절별 기후변화와 작물에 따른 사례별로 개발 모델을 관리하는 등의 다양한 추가 연구가 수행되어야 한다.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2003년도 제2차 연례학술대회 발표논문집
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• pp.277-287
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• 2003

Acetolactate synthase (ALS, EC 4.1.3.18; also referred to as acetohydroxy acid synthase) catalyzes the first common step in the biosynthesis of valine, leucine, and isoleucine in microorganisms and plants. Recently X-ray structure of yeast ALS was available. Pair-wise alignment of yeast and tobacco ALS sequences revealed 63% sequence similarity. Using Deep View and automatic modeling on Swiss model server, we have generated reliable models of tobacco ALS based on yeast ALS template with a calculated pair-wise RMSD of 0.86 Angstrom. Functional roles of four residues located on the subunit interface (H142, El43, M350, and R376) were examined by site-directed mutagenesis. Seven mutants were generated and purified, of which three mutants (H142T, M350V, and R376F) were found to be inactivated under various assay conditions. The H142k mutant showed moderately altered kinetic properties. The E143A mutant increased 10-fold in K$_m$ value while other parameters remained unchanged. The M350C mutant was strongly resistant to three tested herbicides, while the R376k mutant can bind with herbicide carder at similar affinity to that of wild type enzyme, as determined by tryptophan quenching study. Except M350V mutant, all other mutants were ate to bind with cofactor FAD. Taken together, it is likely that residues H142 and E143 are located at the active site, while residues M350 and R376 are possibly located at the overlapping region of active site and herbicide binding site of the enzyme. Our data also allows us to hypothesize that the interaction between side chains of residues M350 and R376 are probably essential for the correct conformation of the active site. It remains to be elucidated that, whether the herbicide, upon binding with enzyme, inactivates the enzyme by causing change in the active site allosterically, which is unfavorable for catalytic activity.

• 한국작물학회지
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• 제52권4호
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• pp.458-468
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• 2007

To tolerate environmentally adverse conditions such as cold, drought, and salinity, plants often synthesize and accumulate proline in cells as compatible osmolytes. ${\Delta}^1$-Pyrroline-5-carboxylate synthetase(P5CS) catalyzes the rate-limiting step of proline biosynthesis from glutamate. Two complete genes, MtP5CS1 and MtP5CS2, were isolated from the model legume Medicago truncatula by cDNA cloning and bacterial artificial chromosome library screening. Nucleotide sequence analysis showed that both genes consisted of 20 exons and 19 introns. Alignment of the predicted amino acid sequences revealed high similarities with P5CS proteins from other plant species. The two MtP5CS genes were expressed in response to high salt and low temperature treatments. Semi-quantitative reverse transcription-polymerase chain reaction showed that MtP5CS1 was expressed earlier than MtP5CS2, indicating differential regulation of the two genes. To evaluate the reverse genetic effects of nucleotide changes on MtP5CS function, a Targeting Induced Local Lesions in Genomes approach was taken. Three mutants each were isolated for MtP5CS1 and MtP5CS2, of which a P5CS2 nonsense mutant carrying a codon change from arginine to stop was expected to bring translation to premature termination. These provide a valuable genetic resource with which to determine the function of the P5CS genes in environmental stress responses of legume crops.