• Ocean Systems Engineering
• /
• v.8 no.4
• /
• pp.409-426
• /
• 2018

This paper presents the strengthening of tubular joint by wrapping Carbon fiber reinforced polymer (CFRP) and glass fiber reinforced polymer (GFRP). In this study, total number of layers, stacking sequence and length of wrapping are the different parameters involved when fiber reinforced polymers (FRP) composites are used for strengthening. For this, parameters where varied and results were compared with the reference joint. The best stacking sequence was identified which has the highest value in ultimate load with lesser deflections. For determining the best stacking sequence, numerical investigation was performed on CFRP composites; length of wrapping and number of layers were fixed. Later, the studies were focused on CFRP and GFRP strengthened joint by varying the total number of layers and length of wrapping. An attempt was done to propose a parametric equation from multiple regression analysis, which can be used for CFRP strengthened joints. Hashin failure criteria was used to check the failure of composites. Results revealed that FRP was having a greater influence in the load bearing capacity of joints, and in reducing the deflections and stresses of joint under axial compressive loads. It was also seen that, CFRP was far better than GFRP in reducing the stresses and deflection.

• Bioinformatics and Biosystems
• /
• v.1 no.1
• /
• pp.46-50
• /
• 2006

According to the advancement of experimental techniques in molecular biology, genomic and protein sequence databases are increasing in size exponentially, and mean sequence lengths are also increasing. Because the sizes of these databases become larger, it is difficult to search similar sequences in biological databases with significant homologies to a query sequence. In this paper, we present the N-gram indexing method to retrieve similar sequences fast, precisely and comparably. This method regards a protein sequence as a text written in language of 20 amino acid codes, adapts N-gram tokens of fixed-length as its indexing scheme for sequence strings. After such tokens are indexed for all the sequences in the database, sequences can be searched with information retrieval algorithms. Using this new method, we have developed a protein sequence search system named as ProSeS (PROtein Sequence Search). ProSeS is a protein sequence analysis system which provides overall analysis results such as similar sequences with significant homologies, predicted subcellular locations of the query sequence, and major keywords extracted from annotations of similar sequences. We show experimentally that the N-gram indexing approach saves the retrieval time significantly, and that it is as accurate as current popular search tool BLAST.

• Proceedings of the IEEK Conference
• /
• 2002.07c
• /
• pp.1539-1542
• /
• 2002

In layout of LSI and PWB, block pack- ing problem is very important in order to reduce chip area. Sequence-pair is typical one of conventional pack- ing method and can search nearly-optimal solution by using Simulated Annealing(SA). SA takes huge computation time due to evaluating of various packing results. Therefore, Sequence-pair is not effective enough for fast layout evaluation including estimation of wire length and rotation of every blocks. This paper proposes an efficient block packing method to minimize wire length and chip area. Our method searches an optimal packing efficient- ly by using a cluster growth algorithm with changing the most valuable packing score on packing process.

• Journal of information and communication convergence engineering
• /
• v.5 no.3
• /
• pp.229-232
• /
• 2007

This paper investigates on the use of constant-amplitude zero-autocorrelation (CAZAC) sequence for channel estimation in multiple-input multiple-output (MIMO) system over indoor wireless channel. Since the symbol-length of the conventional 4-phase CAZAC sequence is short, there is a limitation to use it for MIMO system in multipath environments. An algorithm which generates longer CAZAC sequences is proposed to overcome that problem. Flexible symbol-length of 4-phase CAZAC sequences can be made by the proposed algorithm. Therefore appropriate symbol-length of CAZAC sequences could be utilized as preambles in accordance with the number of transmit antennas and channel condition. The effect of the number of CAZAC sequences for channel estimation is presented in terms of mean square error (MSE).

• Physical Therapy Rehabilitation Science
• /
• v.8 no.4
• /
• pp.225-233
• /
• 2019

Objective: The purpose of this study was to examine the possible effects of incorporating short-term slow-abdominal respiration (SAR) into an exercise program, on balance and the cardiac-related autonomic nervous system (ANS). Design: Cross-over repeated measures design. Methods: Fifteen young and healthy adults were randomly assigned into two groups (7 in the C-R group, 8 in the R-C group), each of which carried out both control sequence (C) and respiration-experiment sequence (R) in the inverse order. In the C sequence, the subjects performed passive exercises and a general exercise program (P-GEP). In the R sequence, the subjects received a short-term SAR training session and then performed the respiration incorporated general exercises program (R-RGEP). Before and after both C and R sequences, the length and the area of the displacement of the center of pressure (COP) and heart rate variability parameters were measured. Results: The total length of the COP displacement in the left single-leg-standing condition showed a significantly greater reduction after R-RGEP in the respiration-experiment sequence than after the P-GEP in the control sequence (p<0.05). The mean heart rate was significantly reduced only after R-RGEP in the respiration-experiment sequence (p<0.05) Conclusions: The slow-abdominal-respiration, trained in a simple manner and integrated into the exercise program in a single session, showed partially positive immediate effects on balance stabilization. The decrease in heart rate indicated possible involvement of the parasympathetic ANS activation in the stability, although it is not enough to decide whether it is purely due to the controlled respiration.

• Journal of Crop Science and Biotechnology
• /
• v.10 no.2
• /
• pp.98-105
• /
• 2007

Phylogenetic relationships in Korean watermelons were evaluated by genetic similarity coefficients using 15 SSR(simple sequence repeat), 14 SCAR(sequence characterized amplified region) and 14 CAPS(sequence characterized amplified region) markers. The SSR markers were selected from previously reported melon and watermelon SSRs through testing polymorphisms within a set of commercial $F_1$ varieties. The SCAR and CAPS markers were developed from polymorphic AFLP(amplified fragment length polymorphism) markers between inbred lines 'BN4001' and 'BN4002'. From the AFLP analysis, 105 polymorphic fragments were identified between the inbred lines using 1,440 primer combinations of EcoRI+CNNN and XbaI+ANNN. Based on the sequencing data of these polymorphic fragments, we synthesized sequence specific primer pairs and detected clear and reliable polymorphisms in 27 primer pairs by indels(insertion/deletion) or RFLP(restriction fragment length polymorphism). A total of 43 sequence-based PCR markers were obtained and polymorphic information content(PIC) was analyzed to measure the informativeness of each marker in watermelon varieties. The average PIC value of SCAR markers was 0.41, which was similar to that of SSR markers. Genetic diversity was also estimated by using these markers to assess the phylogenetic relationships among commercial varieties of watermelon. These markers differentiated 26 Korean watermelon varieties into two major phylogenetic groups, but this grouping was not significantly correlated with their morphological and physiological characteristics. The mean genetic similarity was 66% within the complete set of 26 commercial varieties. In addition, these sequence-based PCR markers were reliable and useful to identify cultivars and genotypes of watermelon.

• The Journal of the Korean Society for Microbiology
• /
• v.34 no.6
• /
• pp.561-571
• /
• 1999

Diagnosis of mycobacterial infection is dependent upon the isolation and identification of causative agents. The procedures involved are time consuming and technically demanding. To improve the laborious identification process mycobacterial systematics supported by gene analysis is feasible, being particularly useful for slowly growing or uncultivable mycobacteria. To complement genetic analysis for the differentiation and identification of mycobacterial species, an alternative marker gene, rpoB encoding the ${\beta}$ subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 52 reference strains of mycobacteria including Mycobacterium tuberculosis H37Rv (ATCC 27294) and clinical isolates by the PCR. The nucleotide sequences were directly determined (306 bp) and aligned using the multiple alignment algorithm in the MegAlign package (DNASTAR) and MEGA program. A phylogenetic tree was constructed with a neighborhood joining method. Comparative sequence analysis of rpoB DNA provided the basis for species differentiation. By being grouped into species-specific clusters with low sequence divergence among strains belonging to same species, all the clinical isolates could be easily identified. Furthermore RFLP analysis enabled rapid identification of clinical isolates.

• Journal of Photoscience
• /
• v.7 no.2
• /
• pp.73-78
• /
• 2000

To analyze molecular traits determining pigmentation between Pharbitis nill violet and white, Amplified Fragment Length Polymorphism(AFLP) and Differential Display Reverse Transcription(DDRT) experiments were carried out with either genomic DNAs or total RNAs isolated from both plants. Results of AFLP experiment in combination of 8 EcoRⅠ primers with 6 MseⅠ primers showed 41 violet-and 60 white-specific DNA bands. In the subsequent experiment, 22 violet-and 22 white-specific DNA fragments were amplified by PCR with DNAs eluted. The sizes of the fragments range from 200 to 600bp. DDRT using total RNA produced 19 violet-and 17 white-specific cDNA fragments, ranging from 200 to 600bp. The fragments obtained by both AFLP and DDRT had been cloned into pGEM T-easy vector, amplified and subjected to the nucleotide sequence analyses. As a result of Blast sequence analysis, most of them sequenced up to date showed no similarity to any Known gene, while few has similarity to known animal or plant genes. An AFLP clone V6, for example, has a strong sequence similarity to the human transcription factor LZIP-alpha mRNA and a DDRT clone W19 to Solanum tuberosum 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA.

• The Plant Pathology Journal
• /
• v.24 no.3
• /
• pp.361-364
• /
• 2008

The complete nucleotide sequences of a Korean isolate of Potato virus X(PVX-Kr) has been determined. Full-length cDNA of PVX-Kr has been directly amplified by long template reverse transcription and polymerase chain reaction(RT-PCR) using virus specific 5'-end primer and 3'-end primer, and then constructed in a plasmid vector. Consecutive subclones of a full-length cDNA clone were constructed to identify whole genome sequence of the virus. Total nucleotide sequences of genome of PVX-Kr were 6,435 excluding one adenine at poly A tail, and genome organization was identical with that of typical PVX species. Comparison of whole genome sequence of PVX-Kr with those of European and South American isolates showed 95.4-96.8% and 77.4-77.9%, in nucleotide similarity, respectively. Sequenced PVX-Kr in this study and twelve isolates already reported could be divided into two subgroups in phylogeny based on their complete nucleotide sequences. Phylogenetic tree analysis demonstrated that PVX-Kr was clustered with European and Asian isolates(Taiwan, os, bs, Kr, S, X3, UK3, ROTH1, Tula) in the same subgroup and South American isolates(CP, CP2, CP4, HB) were clustered in the other subgroup.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.25 no.1A
• /
• pp.10-17
• /
• 2000

In this paper, we evaluate the correlation properties and the optimization of PN sequence phase for multi-media DS/CDMA system with variable spreading gain (VSG) scheme. In multi-media multi-rate DS/CDMA systems, the optimization of PN sequence phase is not a tractable problem, since the sequences should be optimized against both sequences of the same length and other sequences with different length. Hence, we verify the correlation properties of PN sequence phase in multi-rate system environment and furthermore, we propose the new phase criterion, MIN-AIP (minimum-average interference parameter), to minimize the bit error rate (BER). As the results of performance evaluations, it is shown that the performance of MIN-AIP criteria gives the best results.