• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.313-315
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• 2007

Seventeen haplotypes were detected from the complete mitochondrial DNA control region sequences analyzed from eighty individuals of two Tibetan domestic sheep breeds. The nucleotide composition of all the sequences was 33.0% A, 29.7%T, 22.9%C and 14.4%G; G+C was 37.3%. The length of the sequences ranged from 1,107 bp to 1,259 bp. The difference between them was primarily due to 3-5 copy numbers of a 75 bp tandem repeat sequence. The NJ phylogenetic tree (the number of replications of bootstrap test is 1,000) presented three major domestic sheep lineages, which suggested that modern Tibetan sheep breeds are derived from three maternal sources.

• 한국균학회지
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• 제48권1호
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• pp.47-53
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• 2020

During a survey of fungal diversity in different provinces of South Korea in 2017, a new fungal isolate was discovered. This fungal isolate was identified as Westerdykella reniformis, based on its morphological characteristics and phylogenetic analysis, using internal transcribed spacer (ITS) and 28S ribosomal DNA (28S rDNA) sequence data. To our knowledge, W. reniformis has not previously been reported in South Korea. Thus, in this study, we report a new record of a species from the Dothideomycetes class in Korea, and provide a detailed description with morphological illustrations.

• Mycobiology
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• 제42권4호
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• pp.416-421
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• 2014

In this study, we examined arbuscular mycorrhizal fungal (AMF) community structure colonizing field-cultivated ginseng roots according of different ages, such as 1- to 5-year-old plant, collected from Geumsan-gun, Korea. A total of seven AMF species namely, Funnelliformis caledonium, F. moseae, Gigaspora margarita, Paraglomus laccatum, P. occultum, Rhizophagus irregularis, and Scutellospora heterogama were identified from the roots using cloning, PCR-restriction fragment length polymorphism and sequence analysis of the large subunit region in rDNA. AMF species diversity in the ginseng roots decreased with the increase in root age because of the decreased species evenness. In addition, the community structures of AMF in the roots became more uniform. These results suggest that the age of ginseng affects mycorrhizal colonization and its community structure.

• ETRI Journal
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• 제26권5호
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• pp.405-412
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• 2004

MC-CDMA, a multicarrier (MC) modulation scheme based on code division multiple access (CDMA), is the most likely candidate for the next generation of mobile radio communications. The rate compatible punctured turbo (RCPT) coded hybrid automatic repeat request (HARQ) has been found to give improved throughput performance in a direct sequence (DS) CDMA system. However, the extent to which the RCPT HARQ improves the throughput performance of an MC-CDMA system has not been fully understood. In this paper, we apply the RCPT HARQ to MC-CDMA and evaluate by computer simulations its performance in a frequency selective Rayleigh fading channel. We found that the performance of RCPT HARQ MC-CDMA is almost insensitive to channel characteristics. The performance can be drastically improved with receive diversity combined with space-time transmit diversity. In addition, the comparison of RCPT HARQ MC-CDMA, orthogonal frequency division multiplexing, and DS-CDMA shows that under similar conditions the throughput of MC-CDMA is the best in a frequency selective fading channel.

• Journal of Species Research
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• 제6권3호
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• pp.207-213
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• 2017

In order to investigate the indigenous prokaryotic species diversity of the Nakdong River system in Korea, fresh water samples from Hapcheonho Lake and Jinyangho Lake were analyzed for bacterial taxonomic diversity. The isolated bacterial strains were identified based on 16S rRNA gene sequences, and those exhibiting at least 98.7% sequence similarity with known bacterial species, but never reported in Korea, were selected as unrecorded species. Eleven unrecorded bacterial species were discovered in this study. The isolates were identified as Aquabacterium citratiphilum, Clostridium ghonii, Curvibacter delicates, Deinococcus depolymerans, Eubacterium moniliforme, Flavobacterium nitrogenifigens, Kineosporia mesophila, Luteibacter jiangsuensis, Microbacterium terricola, Rhizobium larrymoorei, and Sediminicoccus rosea belonging to the phyla Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria and Deinococcus-Thermus. The selected isolates were further characterized for cellular and colonial morphologies, growth conditions, physiological properties, and enzymatic activities. Descriptive information of these previously unrecorded species is also provided.

• Journal of Microbiology and Biotechnology
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• 제18권5호
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• pp.815-820
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• 2008

Two culture-independent methods, namely ribosomal DNA libraries and denaturing gradient gel electrophoresis (DGGE), were adopted to examine the microbial community of a Malaysian light crude oil. In this study, both 16S and 18S rDNAs were PCR-amplified from bulk DNA of crude oil samples, cloned, and sequenced. Analyses of restriction fragment length polymorphism (RFLP) and phylogenetics clustered the 16S and 18S rDNA sequences into seven and six groups, respectively. The ribosomal DNA sequences obtained showed sequence similarity between 90 to 100% to those available in the GenBank database. The closest relatives documented for the 16S rDNAs include member species of Thermoincola and Rhodopseudomonas, whereas the closest fungal relatives include Acremonium, Ceriporiopsis, Xeromyces, Lecythophora, and Candida. Others were affiliated to uncultured bacteria and uncultured ascomycete. The 16S rDNA library demonstrated predomination by a single uncultured bacterial type by >80% relative abundance. The predomination was confirmed by DGGE analysis.

• Mycobiology
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• 제45권4호
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• pp.353-361
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• 2017

During a survey of fungal diversity of the order Hypocreales in Korea, two Acremonium isolates, CNUFC-1YSRS2-4 and CNUFC-GSNPF3-1, were isolated from soils collected on a bank of the Yeongsan River, Naju, and in a forest on the Mt. Daegak located on Sinsi Island, Gunsan, South Korea, respectively. Based on the morphological characteristics and sequence analysis of the internal transcribed spacer and D1/D2 domains of 28S ribosomal DNA, the isolates CNUFC-1YSRS2-4 and CNUFC-GSNPF3-1 were identified as A. variecolor and A. persicinum, respectively. These 2 species represent novel Hypocreales isolates in Korea.

• The Plant Pathology Journal
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• 제35권4호
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• pp.389-392
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• 2019

The studies on detection of the Strawberry mottle virus (SMoV) have been conducted in Poland for breeding programme purpose and for producers of strawberry plant material. Leaf samples collected from infected strawberry plants were grafted on Fragaria sp. Indicators which were maintained in greenhouse for further study. Seven Fragaria vesca var. semperflorens 'Alpine' indicators infected by SMoV were used for the study aimed on molecular characterization of virus isolates. Partial RNA2 was amplified from total nucleic acids using the RT-PCR method. The obtained amplicons separately digested with BfaI, FauI, HaeIII, HincI, and TaqI enzymes showed different restriction profiles. The nucleotide sequences analysis of RNA2 fragment confirmed the genetic diversity of the SMoV isolates as their similarity ranged from 94.7 to 100%. Polish isolates shared 75.7-99.2% identity with sequence of the virus strains from the Czech Republic, the Netherlands, and Canada. Phylogenetic analysis resulted in grouping of the isolates found in Poland together with one of the Czech strain whereas two other from the Czech and the strains from the Netherlands and Canada created the separate cluster.

• Journal of Microbiology
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• 제44권5호
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• pp.572-576
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• 2006

To obtain primary idea on oral bacterium species that are generally present in periodotally healthy Koreans, the oral bacterial flora in the saliva of four periodontally healthy Koreans at different ages (5, 32, 35, 65) was investigated in this study. For this investigation, 16S rRNA gene clone libraries were generated from the saliva of the four healthy Koreans, and 50 clones were randomly selected from each saliva clone library and sequenced. Totally, 37 different kinds of bacterial 16S rRNA gene sequences were identified based on sequence homology search through GenBank database. The 37 kinds of saliva clone sequences were classified to 14 genera and 2 uncultured and 1 unidentified bacteria. Among the 14 identified genera, Streptococcus, Prevotella, and Veillollella were common genera, and Streptococcus was dominant genus that accounted for 7 different species. Among the seven Streptococcus species, S. salivarius appeared as the most common species. More numbers of species belonging to the genera Streptococcus and Prevotella was present in saliva from ages 32 and 35. While saliva from ages 5 and 65 showed more numbers of species belonging to the genera Rothia, including potential pathogenic species. Overall, saliva of a young child and a senior showed higher bacterial diversity than that of young adults.

• Journal of Forest and Environmental Science
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• 제24권1호
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• pp.27-34
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• 2008

Level and distribution of genetic diversity in seven populations of Albizia lucida Benth. in eastern region of the Indian sub-continent were estimated using ISSR markers. Relatively higher level of genetic diversity within populations was observed in seven populations of A. lucida (mean of 0.38). From the result of AMOVA, majority of genetic diversity was allocated within populations (96.2%) resulting in a moderate degree of population differentiation. The observed distribution pattern of I-SSR variant among the populations was coincided with the typical pattern of long-lived woody tree species. Genetic relationships among the populations, reconstructed by UPGMA method, revealed two genetic groups. The population of Anugul and Bargarh turned out to be the most closely related despite a distance location between them. These formations will be of great value in the development of conservation plans for species exhibiting high levels of genetic differentiation due to fragmentation, such as indication of conservation unit size, which populations should be chosen as priority in conservation plans and which samples should be introduced in areas with a low number of individuals of A. lucida.