• Journal of Microbiology
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• 제33권3호
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• pp.260-266
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• 1995

Hepatitis C virus (HCV) has been considered as a mojor causative agent of post-transfusion related non-A, non-B hepatitis. In this study, the cDNA sequence of NS4 region of HCV (HCV-S) obtained from a Korean patient's plasms was determined. Comparative nucleotide sequence analysis between to type II. 67.2% homology to type III, and 66.4% homology to type IV. The putative amino acid sequence homologies to types I, II, III, and IV were 82.8-84.7%, 92.5-95.1%. 72.5, and 71.1%, respectively. This data strongly suggests that HCV-S should be classified as type II. Significant similarities of hydrophobicity profiles and putative transmembranous domains were found in HCV-S and four major prototypes, indicating that the protein structure is similar in spite of the heterogeneities of intertype homologies at the level of the psrimary nucleotide and amino acid sequences.

• Applied Biological Chemistry
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• 제36권3호
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• pp.208-214
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• 1993

엽록체 DNA 내에서 반복 염기서열의 존재와 이들에 의한 유전자 재배열 현상을 고찰하기 위하여 151bp Repeated Sequence 갖는 이질적인 유전인자군의 존재를 여러가지 품종의 벼 엽록체 DNA에서 관찰 하였다. 또한 쌀 DNA를 벼의 생장과 조직부위에 따라 분리하고, rp12 probe를 이용하여 Southern blot 분석하여 엽록체의 발달에 따르는 엽록체 DNA의 재배열 현상을 관찰하였다. 아울러 유전자 재배열 현상을 유발하는 반복염기서열을 database로부터 검색하여 유전자의 상호 비교 분석하였다. 그 결과 151bp Repeated Sequence와 유사한 염기 서열을 같는 rp123유전자를 포함하는 이질적인 유전인자군은 어느 특정한 품종의 벼에 국한되는것이 아니고 본 실험에 사용된 다양한 품종의 벼에 일반적으로 나타나는 현상임이 확인되었으며 또한 이들의 양상은 벼의 조직 부위에 따라 다르게 나타나고 있음을 확인하였다. 이러한 실험적 결과와 함께 엽록체 유전자 database의 검색과 유전자의 상호비교분석을 통하여 151bp 반복 염기 저열에 의한 벼 엽록체 DNA의 유전자 재배열현상은 식물 특히 단자엽 식물의 진화와 함께 발달된 현상으로 특히 151bp반복 염기 서열은 매우 다양한 유전자 재배열을 유발하는 변이유발 위치로 발달되어 왔음을 확인할 수 있었다. 따라서 이러한 반복염기서열에 의한 유전자 재배열 현상은 특히 벼에 있어서 plastid의 발달에 밀접하게 관여하고 있음을 제시하고 있다.

• Parasites, Hosts and Diseases
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• 제53권3호
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• pp.345-348
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• 2015

Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.

• Asian-Australasian Journal of Animal Sciences
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• 제6권4호
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• pp.541-547
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• 1993

A phage clone containing the partial ${\alpha}_{S1}$-casein gene was isolated from a bovine genomic library by using mixed probes of ovine ${\alpha}_{S1}$-, ${\beta}$- and ${\kappa}$-casein cDNAs. Restriction enzyme mapping analysis for 14.6 kb revealed that the map was in conflict with the report of Meade et al. (1990), especially in the 3'-end fragment. Sequence analysis of 12.6 kb revealed a high AT/GC ratio (1.64); we have identified eight exon sequences according to the bovine ${\alpha}_{S1}$-casein cDNA sequence. The same exon/intron splice junction sequence was observed between these exons. We suggest that the bovine ${\alpha}_{S1}$-casein gene night contain a minimum of 18 exons and the full length is approximately 18-19 kb.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권2호
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• pp.191-199
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• 2001

We have constructed a cDNA library from the whole body of the spider, Araneus ventricosus. Sequence analysis of randomly selected cDNA clones was performed to obtain genetic information on the spider A. ventricosus, of which genetic information is currently not available. We have partially sequenced randomly selected 385 clones of the cDNA library constructed from A. ventricosus. This expressed sequence tags (EST) analysis revealed 383 genes had high homologies to known genes in GenBank. In this report, 241 independent genes were analyzed in detail.

• Food Science and Biotechnology
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• 제27권6호
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• pp.1755-1760
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• 2018

The objective of this study was to perform genetic diversity analysis of 13 strains isolated from South Korean foods by multilocus sequence typing (MLST). For typing, seven housekeeping loci (atpA, dnaA, dnaK, gyrB, pheS, pyrG, and rpoA) were selected, amplified and analyzed. Fifty-one polymorphic sites varying from 1 to 22 in each species were identified. Thirteen sequence types were generated with allele numbers ranged from 2 to 10. The overall relationship between strains was assessed by unweighted pair group method with arithmetic mean dendrogram and minimum spanning tree. In addition, combined spits tree analysis revealed intragenic recombination. No clear relationship was observed between the isolation sources and strains. The developed MLST scheme enhanced our knowledge of the population diversity of Leu. citreum strains and will be used further for the selection of industrially important strain.

• 한국정보과학회 언어공학연구회:학술대회논문집(한글 및 한국어 정보처리)
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• 한국정보과학회언어공학연구회 2020년도 제32회 한글 및 한국어 정보처리 학술대회
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• pp.414-417
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• 2020

최근 한국어 형태소 분석 및 품사 태깅에 관한 연구는 주로 표층형에 대해 형태소 분리와 품사 태깅을 먼저하고, 추가 언어자원을 사용하여 후처리로 형태소 원형과 품사를 복원해왔다. 본 연구에서는 형태소 분석 및 품사 태깅을 두 단계로 나누어, Sequence-to-Sequence를 활용하여 형태소 원형 복원을 먼저 하고, 최근 자연어처리의 다양한 분야에서 우수한 성능을 보이는 BERT를 활용하여 형태소 분리 및 품사 태깅을 하였다. 본 논문에서는 두 단계를 파이프라인으로 연결하였고, 제안하는 형태소 분석 및 품사 태깅 파이프라인 모델은 음절 정확도가 98.39%, 형태소 정확도 98.27%, 어절 정확도 96.31%의 성능을 보였다.

• 정보보호학회논문지
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• 제27권1호
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• pp.91-101
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• 2017

API(Application Program Interface) 콜 시퀀스 분석은 분석 대상 프로그램에서 API 콜 정보를 추출한 후 분석하는 기법으로 다른 기법들에 비해 대상의 행위를 특징할 수 있는 장점이 있다. 하지만 기존의 API 콜 시퀀스 분석기법은 동일한 기능을 수행하는 함수를 상이한 함수로 잘못 식별하여 분석을 수행하는 문제점이 존재한다. 본 연구에서는 API 각각을 추상화시키는 방식을 추가하여 기존의 식별 문제를 해결하고 분석 성능을 향상시키고자 한다. 그 후 분석 대상들에서 획득한 추상화된 API 콜 시퀀스에 LSH(Locality Sensitive Hashing) 기법을 적용하여 각 분석 대상들 간의 유사도를 계산하고 유사한 유형끼리 클러스터를 형성하는 과정을 수행하였다. 본 연구는 악성코드 분석 시 악성코드의 유형을 파악하는 데 요긴하게 사용할 수 있으며, 최종적으로는 해당 유형 정보를 기반으로 악성코드 분석의 정확도를 향상시키는 데 기여할 수 있다.

• Structural Monitoring and Maintenance
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• 제5권2호
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• pp.173-187
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• 2018

Performance evaluation of RC frame building by nonlinear static pushover analysis that accounts for elastic and post elastic behavior is becoming very popular as a valid decision making tool in seismic hazard resistant designs. Available literature suggests great amount of interest has shown by researchers in suggesting refinements to geometric and material modelling to bridge the gap between analytical predictions and observed performances. Notwithstanding the attempts gaps still exists. Sequence of plastic hinge formation which has great influence on pushover analysis results is an area less investigated. This paper attempts to highlight the importance of hinge sequence considerations to make analysis results more meaningful. Variation in analysis results due to different hinge sequences have been quantified, compared and bounds on analysis results have been presented.

• 대한미생물학회지
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• 제34권6호
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• pp.561-571
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• 1999

Diagnosis of mycobacterial infection is dependent upon the isolation and identification of causative agents. The procedures involved are time consuming and technically demanding. To improve the laborious identification process mycobacterial systematics supported by gene analysis is feasible, being particularly useful for slowly growing or uncultivable mycobacteria. To complement genetic analysis for the differentiation and identification of mycobacterial species, an alternative marker gene, rpoB encoding the ${\beta}$ subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 52 reference strains of mycobacteria including Mycobacterium tuberculosis H37Rv (ATCC 27294) and clinical isolates by the PCR. The nucleotide sequences were directly determined (306 bp) and aligned using the multiple alignment algorithm in the MegAlign package (DNASTAR) and MEGA program. A phylogenetic tree was constructed with a neighborhood joining method. Comparative sequence analysis of rpoB DNA provided the basis for species differentiation. By being grouped into species-specific clusters with low sequence divergence among strains belonging to same species, all the clinical isolates could be easily identified. Furthermore RFLP analysis enabled rapid identification of clinical isolates.