• 한국과학교육학회지
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• 제27권2호
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• pp.144-152
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• 2007

In this study, high school science teachers' and students' various conceptions related to osmosis phenomena were compared with and analyzed in relation to the content of science textbooks used in high school science classrooms and college science courses. The questionnaires developed by the researchers were administered to science teachers and students. Differences can be found between the explanations of science textbooks on osmotic pressure and semi-permeable membranes. Many science teachers and students thought of osmotic pressure as 'membrane pressure occurred by the movement of a solvent'. Moreover, the types of teachers of semi-permeable membranes were similar regardless of their academic majors. Many of the teachers thought of a semi-permeable membrane as a membrane that 'passes small-size particles'; however, many students thought of this type of membrane as being 'selectively permeable'. Also, the salt-pickling cabbage phenomenon seemed to cause significant confusion to science teachers and students. These study results show that teachers and students possess various conceptions related to the osmosis phenomena. These different conceptions related to osmosis phenomena might cause confusion and diverse conceptions including misconceptions among teachers and students.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제13권2호
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• pp.123-140
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• 2009

The effect of a solute concentration difference on the osmotic transport of water through the semi-permeable membrane of a simple cell model is investigated. So far, most studies on osmotic phenomena are described by simple diffusion-type equations ignoring all fluid motion or described by Stokes flow. In our work, as the governing equations, we consider the coupled full Navier-Stokes equations which describe the fluid motion and the full transport equation that takes into account of convection and diffusion effects. A two dimensional finite difference model has been developed to simulate the velocity field, concentration field, and semi-permeable membrane movement. It is shown that the cell swells to regions of lower solute concentration due to the uneven water flux through the semi-permeable membrane. The simulation is applied on a red blood cell geometry and the relevant results are presented.

• Molecules and Cells
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• 제22권1호
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• pp.70-77
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• 2006

Platelets are anucleate cytoplasmic fragments derived from bone marrow megakaryocytes, and endothelial cells constitute the barrier between bloodstream and adjacent tissues. Although platelets are thought to regulate the biological functions of endothelial cells, the molecular mechanisms involved are poorly understood. With human umbilical vein endothelial cells and freshly isolated platelets, we established an in vitro model of platelet-induced endothelial cell proliferation. Platelets stimulated endothelial cell proliferation in a dose-dependent manner and transwell experiments with semi-permeable membranes suggested that direct cell-to-cell contacts were required. We developed mAbs against platelets and selected a mAb that blocks their proliferative effect. We purified the antigen by immunoprecipitation and identified it by Q-TOF MS analysis as the tetraspanin CD9. Since both platelets and endothelial cells expressed CD9 strongly on their surfaces we carried out a pre-treatment experiment that showed that CD9 molecules on the endothelial cells participate in the mitogenic effect of the platelets. The inhibitory effect of our mAb was comparable to that of a well-known functional anti-CD9 mAb. These results suggest that the tetraspanin CD9 plays an important role in endothelial regeneration.