• Title/Summary/Keyword: semi-micro bore column

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Determination of Vitamin $B_{12}$ in Foods Using Column-Switching Technique in $\mu$-HPLC ($\mu$-HPLC의 Column-Switching 기술을 이용한 식품중 비타민 $B_{12}$의 분석)

  • 박성진;김혜경;함태식;김병용
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.6
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    • pp.1208-1211
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    • 1999

  • Semi HPLC using a column switching technique was used to determine the trace content of vitamin B12 in various foods. Total analytical time required less than 20 mins per sample and the recovery ratio was 99.9, 99.6, 100.1 and 99.8% for 1.0, 10.0, 100.0 and 1,000 g/kg, respectively. The content of vitamin B12 in various foods obtained using column switching method showed higher levels compared to labels in dried milk(0.5 g/100g) and in grain products(0.51~34.36 g/100g). Thus, this column switching method was more sensitive, effective and precise than the microbiological analysis currently used to determine the trace compounds like a vitamin B12.

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