• Title/Summary/Keyword: semi-continuous

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Improvement of $\gamma-Aminobutyric$ Acid (GABA) Production Using Cell Entrapment of Lactobacillus brevis GABA 057

  • Choi Soo-Im;Lee Jae-Won;Park Sang-Min;Lee Moo-Young;Ji Geun-Eog;Park Myeong-Soo;Heo Tae-Ryeon
    • Journal of Microbiology and Biotechnology
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    • v.16 no.4
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    • pp.562-568
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    • 2006
  • GABA $(\gamma-aminobutyric\;acid)$ is the principal inhibitory neurotransmitter in the brain. For the efficient production of GAB A, a semi continuous cell entrapment system using Lactobacillus brevis GABA 057 was optimized, including the substrate concentration, bead-stabilizing additives, and reaction conditions. The converted monosodium glutamate (MSG), which was added as a substrate for glutamic acid decarboxylase (GAD), increased from 2% (w/v) to 12% (w/v). The addition of isomaltooligosaccharide to the alginate beads also increased the stability of the entrapped L. brevis and GABA productivity. Consequently, when optimal conditions were applied, up to 223 mM GABA could be produced from 534 mM MSG after 48 h of reaction by using alginate-beadencapsulated L. brevis GABA 057.

Potential Use of Microalgae Scenedesmus acuminatus for Tertiary Treatment of Animal Wastewater (축산폐수 고도처리를 위한 미세조류 Scenedesmus acuminatus의 이용 가능성)

  • Park, Ki-Young;Lim, Byung-Ran;Lee, Ki-Say;Lee, Soo-Koo
    • Journal of The Korean Society of Agricultural Engineers
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    • v.53 no.1
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    • pp.63-69
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    • 2011
  • The green algae Scenedesmus acuminatus was cultured in different media: animal wastewater and an artificial culture medium in order to evaluate potential use for tertiary treatment. The experiments were conducted with air flowrate 1~2 L/min at $28{\sim}30^{\circ}C$. The nitrogen and phosphorus showed very similar removal efficiencies (68~77 % and 69~80 % for nitrogen and phosphorus respectively). The optimal fed period was estimated as three days in the semi-continuous experiment. The effects of $CO_2$ (4.5 %) injection on nutrient uptake from animal wastewater (biological treatment effluent) were compared to an air injection under the same conditions of light and photoperiod. The uptake rates of nutrient with air injection were observed 0.009 gN/gChl-a/day, 0.028 gN/gChl-a/day and T-P 0.003 gP/gChl-a/day for nitrate, total nitrogen and phosphorus respectively. The rates were enhanced by addition of $CO_2$ to 0.026 gN/gChl-a/day, 0.076 gN/gChl-a/day and T-P 0.018 gP/gChl-a/day. This study establishes that $CO_2$ addition during nutrient deprivation of microalgal cells may accelerate tertiary wastewater treatment.

A Study on Countermeasure Strategy on Risk of Human Errors driven by Advanced and Automated Systems Through Consideration of Related Theories (현대의 고도화, 자동화된 시스템이 파생한 휴먼에러에 관한 이론적 고찰을 통한 리스크 대응전략 설정)

  • Shin, In Jae
    • Journal of the Korean Society of Safety
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    • v.29 no.1
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    • pp.86-92
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    • 2014
  • This paper provides an integrated view on human and system interaction in advanced and automated systems, which adopting computerized multi-functional artifacts and complicated organizations, such as nuclear power plants, chemical plants, steel and semi-conduct manufacturing system. As current systems have advanced with various automated equipments but human operators from various organizations are involved in the systems, system safety still remains uncertain. Especially, a human operator plays an important role at the time of critical conditions that can lead to catastrophic accidents. The knowledge on human error helps a risk manager as well as a designer to create and control a more credible system. Several human error theories were reviewed and adopted for forming the integrated perspective: gulf of execution and evaluation; risk homeostasis; the ironies of automation; trust in automation; design affordance; distributed cognition; situation awareness; and plan delegation theory. The integrated perspective embraces human error theories within three levels of human-system interactions such as affordance level, psychological logic level and trust level. This paper argued that risk management process should dealt with human errors by providing (1) reasoning improvement; (2) support to situation awareness of operators; and (3) continuous monitoring on harmonization of human system interaction. This approach may help people to understand risk of human-system interaction failure characteristics and their countermeasures.

Development of Improved PC Stair Connections Using U-Rods (U형 강봉을 사용한 PC 계단 접합부 개발)

  • Chang, Kug-Kwan;Seo, Dae-Won;Chun, Young-Soo
    • Land and Housing Review
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    • v.2 no.4
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    • pp.509-516
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    • 2011
  • Compared with the traditional RC system, precast stairs can save construction time, reduce the cost of concrete casting, etc. This paper focuses on an investigation of improved continuous longitudinal joint details for PC stair systems. The performance of the precast concrete stair connections subjected to displacement control cyclic loading is compared with that of the monolithic connection. The developed connection is composed of U-rods and clamp joint metals. This paper proposes precast stair connection with improved structural performance and experimentally evaluates the structural performance of the proposed joints in terms of maximum load, displacement ductiliy, strain, crack and failure modes.

Examination of contact problem between functionally graded punch and functionally graded layer resting on elastic plane

  • Polat, Alper
    • Structural Engineering and Mechanics
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    • v.78 no.2
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    • pp.135-143
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    • 2021
  • In this study, continuous contact problem in the functionally graded (FG) layer loaded with a FG flat punch resting on the elastic semi-infinite plane was analyzed by the finite element method (FEM). It was assumed that the shear modulus and density of the layer and punch varied according to exponentially throughout their depth. FG layer's weight was included to the problem and additionally all surfaces were considered as frictionless. Analysis of FG materials was performed with a special macro which was added to the ANSYS program. Firstly, the shear modulus of the punch was considered to be very rigid and the results of initial separation load (λcr) and distance (xcr) were compared with the analytical solution. Afterwards, results obtained from the contact analysis made according to the inhomogeneity parameters (β, γ) between FG punch-FG layer which had been unprecedented in the literature were discussed. As a result, FG punch's stress values at the punch edges where stress accumulations occurred were found to be smaller than the rigid punch. The security of the structure, longer life of the material and ease of production are directly related to the reduction of the stress values. The results obtained in this study are important in this respect. Also this work is the first study that investigates the effect of FG punch on the FG layer.

Overview of Chlorophyll-a Concentration Retrieval Algorithms from Multi-Satellite Data

  • Park, Ji-Eun;Park, Kyung-Ae;Park, Young-Je;Han, Hee-Jeong
    • Journal of the Korean earth science society
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    • v.40 no.4
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    • pp.315-328
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    • 2019
  • Since the Coastal Zone Color Scanner (CZCS)/Nimbus-7 was launched in 1978, a variety of studies have been conducted to retrieve ocean color variables from multi-satellites. Several algorithms and formulations have been suggested for estimating ocean color variables based on multi band data at different wavelengths. Chlorophyll-a (chl-a) concentration is one of the most important variables to understand low-level ecosystem in the ocean. To retrieve chl-a concentrations from the satellite observations, an appropriate algorithm depending on water properties is required for each satellite sensor. Most operational empirical algorithms in the global ocean have been developed based on the band-ratio approach, which has the disadvantage of being more adapted to the open ocean than to coastal areas. Alternative algorithms, including the semi-analytical approach, may complement the limits of band-ratio algorithms. As more sensors are planned by various space agencies to monitor the ocean surface, it is expected that continuous monitoring of oceanic ecosystems and environments should be conducted to contribute to the understanding of the oceanic biosphere and the impact of climate change. This study presents an overview of the past and present algorithms for the estimation of chl-a concentration based on multi-satellite data and also presents the prospects for ongoing and upcoming ocean color satellites.

Enhanced and Balanced Microalgal Wastewater Treatment (COD, N, and P) by Interval Inoculation of Activated Sludge

  • Lee, Sang-Ah;Lee, Nakyeong;Oh, Hee-Mock;Ahn, Chi-Yong
    • Journal of Microbiology and Biotechnology
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    • v.29 no.9
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    • pp.1434-1443
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    • 2019
  • Although chemical oxygen demand (COD) is an important issue for wastewater treatment, COD reduction with microalgae has been less studied compared to nitrogen or phosphorus removal. COD removal is not efficient in conventional wastewater treatment using microalgae, because the algae release organic compounds, thereby finally increasing the COD level. This study focused on enhancing COD removal and meeting the effluent standard for discharge by optimizing sludge inoculation timing, which was an important factor in forming a desirable algae/bacteria consortium for more efficient COD removal and higher biomass productivity. Activated sludge has been added to reduce COD in many studies, but its inoculation was done at the start of cultivation. However, when the sludge was added after 3 days of cultivation, at which point the COD concentration started to increase again, the algal growth and biomass productivity were higher than those of the initial sludge inoculation and control (without sludge). Algal and bacterial cell numbers measured by qPCR were also higher with sludge inoculation at 3 days later. In a semi-continuous cultivation system, a hydraulic retention time of 5 days with sludge inoculation resulted in the highest biomass productivity and N/P removal. This study achieved a further improved COD removal than the conventional microalgal wastewater treatment, by introducing bacteria in activated sludge at optimized timing.

Technological Factors Facilitating B40's Motivation in Malaysia to Continue Using Online Crowdsourcing Platform

  • NA'IN, Nuramalina;HUSIN, Mohd Heikal;BAHARUDIN, Ahmad Suhaimi
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.8
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    • pp.117-126
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    • 2021
  • The increasing number of retrenchments because of the current global pandemic, Covid-19, has led many to shift to the digital economy, especially among the low-income group (B40) in Malaysia. Crowdsourcing is the collection of information, opinions, or work from a group of people, usually sourced via the Internet. Fueled by the development of Internet-based platforms that provided its technological foundation, and the need for an agile and uniquely skilled workforce, crowdsourcing has grown from the grassroots, with a burgeoning body of research investigating its many aspects. However, very few studies examined crowd workers' motivation for continuous participation on online crowdsourcing platforms. Thus, this paper aims to explore the technological factors that facilitate B40's group motivation in Malaysia to continue to participate in online crowdsourcing platforms. This paper employed a qualitative approach, using a semi-structured interview. The thematic analysis method was used to decode the data extracted from the interview transcript. The finding of this study identified four main themes and seven sub-themes: (1) Technology efficacy, (2) Platform Management: client-worker management, safety net, payment mechanism, (3) Platform Design: UI design, rating feature and (4) Infrastructure: Internet connection, technology infrastructure. This study can provide a guideline for managing crowdsourcing practices in Malaysia, especially for the crowdsourcing platform developer.

Effect of bolt preloading on rotational stiffness of stainless steel end-plate connections

  • Yuchen Song;Brian Uy
    • Steel and Composite Structures
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    • v.48 no.5
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    • pp.547-564
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    • 2023
  • This study investigates the effect of bolt preloading on the rotational stiffness of stainless steel end-plate connections. An experimental programme incorporating 11 full-scale joint specimens are carried out comparing the behaviours of fully pre-tensioned (PT) and snug-tightened (ST) flush/extended end-plate connections, made of austenitic or lean duplex stainless steels. It is observed from the tests that the presence of bolt preloading leads to a significant increase in the rotational stiffness. A parallel finite element analysis (FEA) validated against the test results demonstrates that the geometric imperfection of end-plate has a strong influence on the moment-rotation response of preloaded end-plate connections, which is crucial to explain the observed "two-stage" behaviour of these connections. Based on the data obtained from the tests and FE parametric study, the performance of the Eurocode 3 predictive model is evaluated, which exhibits a significant deviation in predicting the rotational stiffness of stainless steel end-plate connections. A modified bi-linear model, which incorporates three key properties, is therefore proposed to enable a better prediction. Finally, the effect of bolt preloading is demonstrated at the system (structure) level considering the serviceability of semi-continuous stainless steel beams with end-plate connections.

Post-buckling analysis of sandwich FG porous cylindrical shells with a viscoelastic core

  • Foroutan, Kamran;Dai, Liming
    • Steel and Composite Structures
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    • v.45 no.3
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    • pp.349-367
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    • 2022
  • In this research, an approach combining a semi-analytical method and an analytical method is presented to investigate the static and dynamic post-buckling behavior of the sandwich functionally graded (FG) porous cylindrical shells exposed to external pressure. The sandwich cylindrical shell considered is composed of a viscoelastic core and two FG porous (FGP) face layers. The viscoelastic core is made of Kelvin-Voigt-type material. The material properties of the FG porous face layer are considered continuous through each face thickness according to a porosity coefficient and a volume fraction index. Two types of sandwich FG porous viscoelastic cylindrical shells named Type A and Type B are considered in the research. Type A shell has the porosity evenly distributed across the thickness direction, and Type B has the porosity unevenly distributes across the thickness direction. The FG face layers are considered in two cases: outside metal surface, inside ceramic surface (OMS-ICS), and inside metal surface, outside ceramic surface (IMS-OCS). According to Donnell shell theory, von-Karman equation, and Galerkin's method, a discretized nonlinear governing equation is derived for analyzing the behavior of the shells. The explicit expressions for static and dynamic critical buckling loading are thus developed. To study the dynamic buckling of the shells, the governing equation is examined via a numerical approach implementing the fourth-order Runge-Kutta method. With a procedure presented by Budiansky-Roth, the critical load for dynamic post-buckling is obtained. The effects of various parameters, such as material and geometrical parameters, on the post-buckling behaviors are investigated.