• Korean Journal of Veterinary Research
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• v.58 no.4
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• pp.201-209
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• 2018

Canine mammary tumors are among the most frequently observed cutaneous tumors in female dogs. Cancer stem cells (CSCs), referred to as tumor-initiating cells, are thought to have properties similar to normal stem cells such as the ability to self-renewal and to differentiate into various cell types. Biological understanding of CSCs and the critical pathways involved in their maintenance are important in research and therapy for mammary tumors. We conducted the present study on sphere formation from REM134 cells by using methylcellulose to produce tumorspheres on a large scale and compared the specific markers of the spheres-formed and plating-cultured REM134 cells. The results revealed that the tumorspheres cultured in methylcellulose had higher seeding density and improved morphology compared to those produced in normal sphere formation medium. Expression levels of stemness markers and CSC-related markers were higher in tumorsphere-forming cells than in plating-cultured cells. Subsequently, we transplanted the tumorsphere-forming and plating-cultured cells into female nude mice to examine their tumorigenic potential. Tumor volume increased rapidly in mice transplanted with tumorsphere-derived cells compared to plating-cultured cells. We observed a novel sphere-forming condition for REM134 cells and showed that REM134 cell tumorspheres can exhibit improved CSC properties.

• Journal of the Korea Convergence Society
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• v.10 no.10
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• pp.267-274
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• 2019

The purpose of this study was to investigate the relationship between parenting attitude and child development. For this purpose, a survey was conducted to parents and children who visited child development centers and child-related welfare institutions located in Seoul and the Seoul metropolitan area. Total 230 pieces of survey papers were prepared, and 183 of them - except for 47 copies which were judged to be unreliable were used for the test analysis. Data analysis was performed using SPSS version 18.0 and descriptive statistical analysis was performed to analyze general characteristics of the subjects. In order to analyze the parenting attitude and developmental relationship of the children, the regression analysis was performed. Parents' parenting attitude - especially pressure and excessive expectation were found to have an effect on self-ridicule behaviors of children, and supervision and excessive expectation were found in small muscle exercise. Achievement pressure and excessive expectation were also the main variables in expression language and language understanding. Achievement pressure, director and excessive expectation were found to have effects on children's cognitive development.

• Molecules and Cells
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• v.44 no.5
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• pp.310-317
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• 2021

Cluster of differentiation 1 (CD1) is a family of cell-surface glycoproteins that present lipid antigens to T cells. Humans have five CD1 isoforms. CD1a is distinguished by the small volume of its antigen-binding groove and its stunted A' pocket, its high and exclusive expression on Langerhans cells, and its localization in the early endosomal and recycling intracellular trafficking compartments. Its ligands originate from self or foreign sources. There are three modes by which the T-cell receptors of CD1a-restricted T cells interact with the CD1a:lipid complex: they bind to both the CD1a surface and the antigen or to only CD1a itself, which activates the T cell, or they are unable to bind because of bulky motifs protruding from the antigen-binding groove, which might inhibit autoreactive T-cell activation. Recently, several studies have shown that by producing T_H2 or T_H17 cytokines, CD1a-restricted T cells contribute to inflammatory skin disorders, including atopic dermatitis, psoriasis, allergic contact dermatitis, and wasp/bee venom allergy. They may also participate in other diseases, including pulmonary disorders and cancer, because CD1a-expressing dendritic cells are also located in non-skin tissues. In this mini-review, we discuss the current knowledge regarding the biology of CD1a-reactive T cells and their potential roles in disease.

• Land and Housing Review
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• v.13 no.4
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• pp.91-103
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• 2022

Home has significant meaning in the real world. In contrast, there's a lack of interest in homes in the Metaverse compared to other architectural spaces. This study aims to establish the concept of a home in the Metaverse based on meaning, typology, and characteristics of real-world homes. For this purpose, previous research and existing models of real- world homes were analyzed and case studies and a survey were conducted of homes in the Metaverse. As a result of the research, a model of home in the Metaverse is proposed based on physical, social, and personal models. Modifying one's dwelling, a refuge from the outside world, and self-expression/personalized space was also identified as the most significant characteristic of a home in the Metaverse. The results of this study will be helpful in future Metaverse virtual world research and development of Metaverse service platforms.

• International Journal of Computer Science & Network Security
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• v.21 no.3
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• pp.21-30
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• 2021

Economic transformations have led to an increase in the role of creative assets and their central role in public life. Changes in creative activity have led to a change in the organization of the work of institutes engaged in the training of specialists, in particular teachers of labor education. Methods and approaches to training determine the development of creative industries, being the basis for models of professional training of future teachers of labor training. The purpose of an article was to develop a modern model of professional training of future teachers of labor training based on the concept of creative economy. The methodology is based on the concepts of holistic craft and creative economy. Based on the integration of pedagogical learning models "Craft as design and problem-solving", "Craft as skill and knowledge building", "Craft as product-making" and "Craft as self-expression" developed and experimentally confirmed the conceptual model of professional training of future teachers of labor training. The proposed model forms a practitioner with professional, technical, digital and creative skills who is able to transfer the experience to students. The training course "Creativity and creative thinking" has been developed. The model provided for the development of a course based on the strategy of developing professional creativity, flexibility, improvisation, openness, student activity, joint practice, student-oriented approach. The practical value implies the adaptation of the developed model of professional training of future teachers of labor education during the training of teachers in higher education, which is confirmed in the experiment.

• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.4
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• pp.177-183
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• 2020

The Hippo-YAP signaling pathway is critical for cell proliferation, survival, and self-renewal in both Drosophila and mammals. Disorder of Hippo-YAP pathway leads to tumor development, progression and poor prognosis in various cancers. YAP/TAZ are the key downstream effectors of the Hippo pathway and they can be inhibited through LATS1/2, core kinases in the Hippo pathway, mediated phosphorylation. In this study, we investigated the effect of Angelica gigas Nakai extract (AGNE) on Hippo-YAP/TAZ pathway. First, ANGE induced YAP/TAZ phosphorylation and dissociation of the YAP/TAZ-TEAD transcription complex. By qRT-PCR, we found that ANGE inhibits the expression of YAP/TAZ-TEAD target gene, CTGF and CYR61. In addition, the transcriptional activity of YAP/TAZ was not suppressed significantly in LATS1/2 double-knockout (DKO) cells by ANGE compared to LATS1/2 wild-type (WT) cells, which means AGNE inhibits YAP/TAZ signaling through direct action on LATS1/2. Further, it was confirmed that AGNE-induced activation of LATS1/2 inhibited the migration potential of the vector-expressing cells by suppressing YAP/TAZ activity. The reduced migration potential was restored in active YAP-TEAD expressing cells. Taken together, the results of this study indicate that ANGE downregulates YAP/TAZ signaling in cells through the activation of LATS1/2.

• Journal of Industrial Convergence
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• v.20 no.6
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• pp.125-133
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• 2022

The purpose of this study was to identify the mediating effects of positive thinking in the relationship between emotional expressivity and public speaking anxiety. Participants were recruited from one university freshmen located in Gwangju city. Data were collected from 146 university freshmen. The self-reported questionnaire was used to assess the level of positive thinking, emotional expressivity, public speaking anxiety. The SPSS WIN 23.0 version program was used with Baron and Kenny's 3-Step mediating effect and Sobel test. It was examined whether there was a mediating effect of positive thinking on emotional expression and presentation anxiety. In the second stage, emotional expressivity showed a significant effect on presentation anxiety. In stage 3, emotional expressivity was not statistically significant in presentation anxiety, and positive thinking was statistically significant in presentation anxiety. Positive thinking had a total mediating effect(z=-4.57, p<.001) in the relationship between emotional expressivity and public speaking anxiety. To reduce the public speaking anxiety among university freshmen, it is necessary to develop interventions that enhance positive thinking in addition to providing emotional expressivity.

• The Journal of the Korea Contents Association
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• v.22 no.7
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• pp.361-369
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• 2022

The purpose of this study was to review the effectiveness of drama program for the people with developmental disabilities in Korea by using method of meta-analysis. At first, master's theses, doctoral dissertations, and journal articles published in Korea up to January, 2022 were reviewed. Resultingly a total of 13 studies were eligible base upon the inclusion criteria. The mean effect sizes and test for homogeneity of effect size (Q-statistic) were analyzed by using Comprehensive Meta-Analysis Software 2.0. The main findings of this study are as follows. First, the average effect sizes for drama program for people with developmental disabilities were ES= 0.8335 for sociality, ES= 0.6512 of self-expression. Second, by analysing the moderate variables of the effect size for drama program for people with developmental disabilities, 'age', 'the number of session' 'the type of drama program', 'the major of drama director' were statistically significant. The research and practice implications of drama programs for people with developmental disabilities in Korea were suggested.

• Molecules and Cells
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• v.45 no.10
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• pp.695-701
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• 2022

Homeostatic regulation of meristematic stem cells accomplished by maintaining a balance between stem cell self-renewal and differentiation is critical for proper plant growth and development. The quiescent center (QC) regulates root apical meristem homeostasis by maintaining stem cell fate during plant root development. Cell cycle checkpoints, such as anaphase promoting complex/cyclosome/cell cycle switch 52 A2 (APC/C^CCS52A2), strictly control the low proliferation rate of QC cells. Although APC/C^CCS52A2 plays a critical role in maintaining QC cell division, the molecular mechanism that regulates its activity remains largely unknown. Here, we identified SCF^FBS1, a ubiquitin E3 ligase, as a key regulator of QC cell division through the direct proteolysis of CCS52A2. FBS1 activity is positively associated with QC cell division and CCS52A2 proteolysis. FBS1 overexpression or ccs52a2-1 knockout consistently resulted in abnormal root development, characterized by root growth inhibition and low mitotic activity in the meristematic zone. Loss-of-function mutation of FBS1, on the other hand, resulted in low QC cell division, extremely low WOX5 expression, and rapid root growth. The 26S proteasome-mediated degradation of CCS52A2 was facilitated by its direct interaction with FBS1. The FBS1 genetically interacted with APC/C^CCS52A2-ERF115-PSKR1 signaling module for QC division. Thus, our findings establish SCF^FBS1-mediated CCS52A2 proteolysis as the molecular mechanism for controlling QC cell division in plants.

• Journal of The Korean Society of Integrative Medicine
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• v.10 no.2
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• pp.95-104
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• 2022

Purpose : This study aimed to investigate the effects of health science majors' communication skills on their adjustment to college life. Methods : The subjects were 336 college students majoring in health science at colleges located in Busan. The survey's questionnaire comprised 38 items, including 15 items for communication skills and 19 items for adjustment to college life. Results : In the gender-based comparison of communication skills and adjustment to college life, female students had better communication skills, with overall higher scores than male students for the understanding others and communication. In the age-based comparison of communication skills and adjustment to college life, students aged 21 to 22 showed the best communication skills, while students aged 18 to 20 exhibited the least communication skills. In terms of adjustment to college life, those aged 25 or older scored the highest, and those aged 21 to 22 scored the lowest. In the school-year-based comparison of communication skills and adjustment to college life, third- and first-year students showed the best and least communication skills, respectively. Overall, third- and fourth-year students were more adjusted at adjusting to college life than first- and second-year students. Third-year students also scored the highest in academic adjustment, whereas second-year students scored the lowest. In terms of relationships between the students' communication skills and their adjustment to college life, communication skills were positively correlated with the following subdomains: understanding of others, self-expression, and communication. Communication skills also showed a positive correlation with adjustment to the college environment, including personal-emotional adjustment. Conclusions : The present study found that communication skills did not significantly affect health science majors' adjustment to college life. However, given that communication skills are an essential factor for effective work performance and greater job satisfaction, it is recommended that colleges provide students with relevant education and experiences to help them enhance communication skills while in school.