• Genomics & Informatics
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• v.2 no.2
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• pp.75-80
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• 2004

In a variety of animal species, the perinatal exposure of experimental animals to the 2,3,7,8-tetrachlorodibenzo­p-dioxin (TCDD) leads to the immune dysfunction, which is more severe and persistent than that caused by adult exposure. We report here the changes of gene expression and the identification of the marker-genes representing the dioxin exposure. The expressions of the transcripts were analyzed using the 11 K oligonucleotide­microarray from the bone marrow cells of male C57BL/6J mice after an intraperitoneal injection of $1{\mu}g$ TCDD/kg body weight at various time intervals: gestational 6.5 day(G6.5), 13.5 day(G13.5), 18.5 day(G18.5), and postnatal 3 (P3W)and 6 week (P6W). The type of self-organizing maps(SOM) representing the specific exposure dioxin could be identified as follows; G6.5D(C14), G13.5D(C0, C5, C10, C18), G18.5D(7): P3W(C2, C21), and P6W(C4, C15, C20). The candidate marker-genes were restricted to the transcripts, which could be consistently expressed greater than $\pm$2-fold in three experiments. The resulting candidates were 85 genes, the characteristics of that were involved in cell physiology and cell functions such as cell proliferation and immune function. We identified the biomarker-genes for dioxin exposure: smc -like 2 from SOM C14 for the dioxin exposure at G6.5D, focal adhesion kinase and 6 other genes from C0, and protein tyrosine phosphatase 4a2 and 3 other genes from C5 for G13.5D, platelet factor 4 from C7 for G18.5D, fos from C2 for P3W.

• Journal of Digital Convergence
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• v.11 no.7
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• pp.305-312
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• 2013

The purpose of this qualitative study is to analyze the experience of multicultural couples who participated in the movie making educational activity within the program "Communication Development among Multicultural and Indigenous Couples Through Intercultural Praxis." This program was held for 8 months from March 10, 2012 to November 11, 2012. Among the participants, 4 couples agreed for both individual and group interviews and provided data for this paper. The interview result can be categorized into the following four: quality relationship, acceptance and support, positive thinking, and accomplishment.

• 모자간호학회지
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• v.4 no.1
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• pp.80-90
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• 1994

Concept analysis is a strategy that examines the attributes or characteristics of a concept. It is a formal linguistic exercise to determine those defining attributes. The basic purpose of concept analysis is to clarify ambiguous concepts in theory, and to propose a precise operational definition which reflects the theoretical base of the concept. The concept of maternal role is too broad and variable in expression, therefore there has been no authentic definition nor standard opinion for this concept. The purpose of this study is to clarify the concept of maternal role which in turn will help in the development of a theory of maternal role attainment. In order to analyze the concept of maternal role, much effort was extended to look up "maternal role" in various dictionaries and literature, but only a few cases were found which listed this terminology. Consultation on the meaning of "mother" and "role" separately was then done and consequently a definition of "maternal role" was obtained through concept analysis of these two words independently, finding their attributes and unifying them. The attributes of the concept of maternal role which was analyzed based on the steps of concept analysis by Walker & Avant (1988) are : 1) Women who have pregnancies 2) Women who have a child 3) Women who rear a child 4) Expected behavior according to social status 5) Learning through social cultures 6) Social interrelationships. The antecedents of the maternal role are : 1) women who are pregnant and deliver a baby, must keep in contact with their child. 2) Circumstances for childrearing must be provided. 3) Formation of self-concept as mother should be developed, cognitive activity, which is necessary for the nurturing activity, financial support and preparation of childrearing should be provided. And the consequences of the maternal role are : 1) Improvement 2) Changeability 3) Ambiguity 4) Stability Through this concept analysis, the concept of the maternal role is defined as "expected behavior by social interrelationships and learned nurturing behavior through social cultures."

• Journal of Ginseng Research
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• v.39 no.4
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• pp.365-370
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• 2015

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been sufficiently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to $200{\mu}M$) for 1 h before treatment with 1 mg/mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

• Journal of Digital Contents Society
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• v.9 no.3
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• pp.389-398
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• 2008

There has been some research into a speech system for communications between those who are hearing impaired and those who hear normally, but the system has been pursued in inefficient teaching ways in which existing teachers teach each individual due to social indifference and a lack of marketability. In order to overcome such a weakness, there appeared to be a need to develop contents utilizing 3D animation and digital technology. For the investigation of a standard face and a standard spherical shape for the preparation of a character, the study collected sufficient data concerning students in the third-sixth grades in elementary schools in Seoul and Gyeonggi, Korea, and drew up standards for a face and a spherical shape of such students. This data is not merely the basic data of content development for the hearing impaired, but it can also offer a standard measurement and a standard type realistically applicable to them. As a system for understanding conversations by applying 3D character animation and educating self-expression, the character speech animation system supports effective learning for language education for hearing impaired children who need language education within their families and in special education institutions with the combination of 3D technology and motion capture.

• The Journal of Korean Academic Society of Nursing Education
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• v.21 no.3
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• pp.320-329
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• 2015

Purpose: The purpose of this study was to identify the relationship among hesitation factor of questions, critical thinking disposition, and problem solving. Methods: Data were collected from 370 students at a university in D city using a structured questionnaire from April 1 to April 15, 2015. It was analyzed with frequency analysis, descriptive statistics, t-tests, ANOVA, and correlation analysis. Results: Subjects scored an average of $2.62{\pm}.52$ for hesitation factor of questions, $3.53{\pm}0.42$ for critical thinking disposition, and $3.44{\pm}0.44$ for problem solving. There were significant differences in the three variables according to satisfaction with major, self-expression, and interpersonal relationships. In addition, hesitation factor of questions exhibited a significant difference between genders. Hesitation factor of questions had a negative relationship with critical thinking disposition (r=-.23, p<.001) and with problem solving (r=-.34, p<.001). In addition, there was a statistically significant positive correlation between critical thinking disposition and problem solving (r=.69, p<.001). Conclusion: In order to improve critical thinking disposition and problem solving, we need to generate a lot of questions to the student's inside. We also need to develop appropriate teaching methods so that this can be expressed by the actual question coming from the control hesitation factor of questions.

• Genomics & Informatics
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• v.2 no.1
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• pp.45-52
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• 2004

The self-splicing group I intron from Tetrahymena thermophila has been demonstrated to perform splicing reaction with its substrate RNA in the trans configuration. In this study, we explored the potential use of the trans-splicing group I ribozymes to replace a specific RNA with a new RNA that exerts any new function we want to introduce. We have chosen thymidine phosphorylase (TP) RNA as a target RNA that is known as a valid cancer prognostic factor. Cancer-specific expression of TP RNA was first evaluated with RT-PCR analysis of RNA from patients with gastric cancer. We determined next which regions of the TP RNA are accessible to ribozymes by employing an RNA mapping strategy, and found that the leader sequences upstream of the AUG start codon appeared to be particularly accessible. A specific ribozyme recognizing the most accessible sequence in the TP RNA with firefly luciferase transcript as a 3' exon was then developed. The specific trans-splicing ribozyme transferred an intended 3' exon tag sequence onto the targeted TP transcripts, resulting in a more than two fold induction of the reporter activity in the presence of TP RNA in mammalian cells, compared to the absence of the target RNA. These results suggest that the Tetrahymena ribozyme can be a potent anti-cancer agent to modify TP RNAs in tumors with a new RNA harboring anti-cancer activity.

• Archives of design research
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• v.17 no.3
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• pp.91-100
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• 2004

Natural forms could be ultimate visual expression power that affects on the industrial design. The rule of power in nature as nature formal characteristics have a direct influence and can be also applied to productional and artificial form. All organic forms and structures in nature have unique form and shape to be self controlled and good in order. Such an order in nature comes from regular and ratio principles which has aesthetical order by mathematics. The specialty of beauty in nature can be revealed not only visual form but also the ratio, balance and rhythm of structural principles. As we examine the aesthetic source, embodied some object can be developed into basic principles. Furthermore, through this study we can find out that the form theory in nature forms share the quality attribute with geometrical form to be shown in industrial product design. In this context, this study aims to make dear visual quality of organic form and structure that is immanent in nature about a form as formative principles of industrial product design, the significancy of this study is to prove the application into functional and structural form of product design.

• Archives of design research
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• v.19 no.2 s.64
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• pp.301-312
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• 2006

The advancement of IT technology and the distribution of PC makes a change on the web contents expressions and user's experiments. The advanced web environment makes virtual web museum. We anticipate that demand of virtual web museum is likely to increase. However, most of studies on web museum are case studies about visual expression or graphic interface of virtual space. This paper will be considered about the functions of digital storytelling on web museum and try to develop an self-evaluation model which can evaluate the value of digital storytelling on web museum. The ultimate goal of this study is to verify the validity of the model to evaluate new storytelling of web museum and to classify the digital storytelling based on the evaluated results, with a view to presenting the direction of development by type. The results of this study may provide a reference of applying digital storytelling into web museum and also it can be a checklist for developing the user experience for navigating a web museum.

• Proceedings of the Korea Contents Association Conference
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• 2008.05a
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• pp.743-748
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• 2008

Design is objective and universal human aesthetic activity. Design originally began with the purpose 'to make public's life abundant and increase their quality of living." However, design education has bee developed to foster human resource to maximize production of company in the name of 'important production method for company.' Therefore, common people has been had many opportunities to learn about design. General people could not be satisfied their demand to learn about design due to narrow opportunities. Public has had resistance feeling for design that it has been exclusively occupied by certain designers. Therefore, it is urgent to expand the object of design education and change the contents which have been focused on fostering design professionals. This research examined about the goal of public design education (self-expression, enjoying quality of life, culture, and wisdom of living) as a way to increase our life quality by recognizing the need of public design education based on various social phenomena which strongly shows people's desire to pursue aesthetic life.