• 한국식품영양과학회지
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• 제44권9호
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• pp.1249-1255
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• 2015

본 연구는 세리신을 이용해 만든 유기셀레늄의 생체이용률을 알아보고자 하였다. 그 결과를 요약하면 다음과 같다. 항산화지표인 환원형 글루타티온은 셀레늄 처치로 인해 증가 되었는데 유기셀레늄에 의한 증가는 무기셀레늄에 비해 큰 경향이었으며, 또한 혈중 과산화 지질도 유기셀레늄이 무기셀레늄에 비해 낮은 값을 나타내어 유기셀레늄은 무기셀레늄에 비해 항산화력 향상에 더 기여하는 것으로 나타났다. 셀레늄 결핍식이로 인해 저하된 셀레늄 흡수율과 보유율은 셀레늄 처치로 증가되는데, 특히 유기셀레늄 처치로 인한 흡수율과 보유율이 높아 생체 내 이용률은 증대될 것으로 사료되며 이는 항산화력 향상에 영향을 미칠 것으로 사료된다. 혈청과 간의 셀레늄의 농도는 셀레늄 처치로 유의하게 증가되는데 셀레늄 형태 중 유기셀레늄에 의한 증가가 가장 컸으나 통계적으로 유의한 수준은 아니었다. 결론적으로 본 연구의 결과 무기질을 함유한 펩타이드는 무기질의 생체이용률을 증가시키는 것으로 나타났는데 세리신을 이용한 유기화 형태로 섭취될 경우 혈액과 장기의 침착뿐 아니라 흡수율과 보유율 증가에 관여하여 각 무기질의 효능을 증진시키는 것으로 나타났다.

• 약학회지
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• 제29권3호
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• pp.144-151
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• 1985

Cathodic stripping voltammetric determination of traces of selenium in plant samples was studied. Stripping peak of selenium (IV) from Cu-Se intermettalic deposit in acidic media containing copper (II) ion is specific, highly sensitive and well defined, is successfully used for the quantitative determination of selenuin down to the level of 1ng/ml. Sample is burnt in a calorimeter bomb under the oxygen pressure of 40atm. and the selenium is absorbed in 0.1M NaOH. After the solution is filtrated, concentrated and acidified with HCl, then passed through a column of cation exchange resin in the $H^{+}$ form(Dowex 50X-8). The column eluate is analyzed for selenium by differential pulse cathodic stripping voltammetric method. Analytical results of selenium for NBS SMR is well agreement with the certified values. Results are given for a series of plant materials.

• Journal of Animal Science and Technology
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• 제59권4호
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• pp.7.1-7.13
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• 2017

Background: Selenium is one of the trace minerals whose deficiency is known to lead to complications of female reproduction. The identified gaps in researches regarding selenium and pregnancy include optimizing the dosage of selenium supplementation, timing of supplementation, finding the best form and type of selenium, and selenium administration combined with other antioxidants. Hence, this study was conceptualized to address one of the identified gaps, that is, to find out the best timing of selenium administration around the time of pregnancy. Specifically, this study aimed to assess the effects of maternal Selenium-supplementation, administered at various stages of periconception period, on murine blastocyst morphology, percent occurrence of good quality blastocysts, and implantation status. Methods: ICR female mice were randomly assigned into the unsupplemented group (Group I) receiving basal diet without selenium, and treatment groups given with $3.0{\mu}g$ selenium-supplement per day during pregestation only (Group II), pregestation-throughout-gestation (Group III) and gestation only (Group IV). Both blastocyst morphology and implantation status were assessed. Results: The morphometric measurements of blastocysts appeared to be unaffected by selenium-supplementation at different stages of periconception. Selenium-supplementation at pregestation only (Group II) and gestation only (Group IV) produced higher percent occurrence of good quality blastocysts and lower percent pre-implantation loss than Group III. Among all the treatment groups, Group III (Selenium-supplementation during pregestation-to-gestation) yielded the lowest quality blastocysts and highest percent pre-implantation loss. Conclusion: Maternal selenium-supplementation during pregestation and gestation stages of the periconception period yielded a high percent occurrence of good quality blastocysts and pre-implantation success.

• Animal Bioscience
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• 제35권11호
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• pp.1725-1732
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• 2022

Objective: This study was conducted to investigate the effects of selenium benefits on the physiological responses, litter performance, blood profiles and milk composition of lactating sows and tissue concentration of their progeny when mixed form of selenium was provided in a lactation diet. Methods: A total of 45 multiparous sows (Yorkshire×Landrace) with similar body weight, backfat thickness, and parity were assigned to one of three treatments with 15 sows per treatment in a completely randomized design. Organic and inorganic selenium were mixed and added to the diet at 0.15 ppm and 0.25 ppm, respectively. A non-Se-fortified corn-soybean meal basal diet served as a negative control. Treatments were as follows: i) Control: corn-soybean meal based diet, ii) ISOS15: control+ inorganic Se 0.15 ppm+organic Se 0.15 ppm, iii) ISOS25: control+inorganic Se 0.25 ppm+organic Se 0.25 ppm. Results: Serum selenium concentrations of sows and piglets were increased by the supplemental Se mixture at 7 days of lactation compared with the control (p<0.01, respectively). The kidney and muscle selenium concentrations of piglets were increased by the supplemental Se mixture at 21 days of lactation compared with the control (p = 0.03; p = 0.04, respectively). Conclusion: Consequently, supplementation with mixed inorganic and organic selenium in a lactating diet could improve the selenium status of sows and piglets; no differences were observed among the mixing levels.

• Asian-Australasian Journal of Animal Sciences
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• 제20권7호
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• pp.1135-1155
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• 2007

Selenium is considered to be one of the most controversial trace elements. On the one hand, it is toxic at high doses and there is a great body of information related to environmental issues of Se contamination. On the other hand, Se deficiency is a global problem related to an increased susceptibility to various diseases of animals and humans and decreased productive and reproductive performance of farm animals. Optimisation of Se nutrition of poultry and farm animals will result in increased efficiency of egg, meat and milk production and even more important, will improve quality. From the data presented in the review it is clear that the main lesson which we have to learn from nature is how to use organic selenium in animal and human diets. Selenium-enriched yeast (Sel-Plex) is the result of such a lesson and it is just a matter of time before animal nutrition moves completely from using ineffective sodium selenite to organic selenium. Other lessons from nature will follow. Recent advances in genomics and proteomics, in association with descriptions of new selenoproteins, will be a driving force in reconsidering old approaches related to Se nutrition. Probably 90% of all Se research has been conducted with sodium selenite and we now understand that the natural form of selenium is different. The main advances in Se status assessment and Se requirements were established based on the activity of glutathione peroxidase (GSH-Px), an enzyme which for many years was considered to be the main selenoprotein. Recently it was discovered that it is only one of at least 25 various selenoproteins. Se research and practical applications are developing quickly and they are very exciting and promising.

• Journal of Nutrition and Health
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• 제26권3호
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• pp.286-298
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• 1993

In order to investigate the effect of selenium (Se) on the liver damage, metallothionein synthesis and hepatic antioxidative detoxification system in cadmium(Cd) administered rats. Sprague-Dawley male rats(60\\5g) were divided into two diet groups, depending on with (CdS groups) or without (Cd groups) 0.5ppm Se supplementation and fed experimental diets ad libidum for 4 weeks. And then each group was again subdivided into five groups, depending on injection number of Cd, i.e., 0, 1, 2, 3, and 4 times of 2.5mg Cd/kg of body wt once a day. Hemoglobin concentration, hematocrit values, superoxide dismutase, glutathione peroxidase and glutathione S-transferase activite were decreased progressively with increasing number of Cd injection, but increased by the supplementation of Se. The reduced form of glutathione (GSH) contents in blood and liver and vitamin E content were decreased and oxidized form (GSSG) increased in Cd groups, but these of Se supplemented groups were not very different from controls. Cd reduced liver vitamin E content which was not restored by Se supplementation. Liver lipid peroxide values were elevated with increasing doses of Cd, but Se supplementation reduced these elevated levels. Accumulation of metallothionein in liver and kidney was increased with increasing number of Cd injection, but Se did not affect on them. Histological examination revealed that lysosomes were significantly increased and mitochondria and Golgi apparatus were enlarged by Cd, however, these changes were reduced by Se. It was concluded that Se administration promoted antioxidative detoxification and alleviated peroxidative damage in rat liver by Cd.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.182.1-182.1
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• 2003

Flavonoids with oxygen atoms are known to have potent biological effect.They have been studied long as major antioxidants which protect cell membranes. Recent medical surveys show that increased intake of selenium decreases the risk of breast, colon, lung and prostrate cancer by preventing free radical generation. The flavonoids, isoflavonoids, and coumarins which form the bulk of these compounds are very polar and have limited use as drugs which have to pass through BBB(Brain Blood Barrier)The non-polar property is increased by exchange oxygen to selenium as a part of heterocyclic compound. (omitted)

• 한국초지조사료학회지
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• 제33권4호
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• pp.281-289
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• 2013

본 연구는 다양한 수준의 셀레늄 강화 청보리 사일리지와 무기셀레늄을 급여 시 비육기 거세한우의 생산성, 혈액 성상, 도체특성 및 조직 내 셀레늄 축적에 미치는 영향을 조사하였다. 시험은 거세비육우 20두를 공시하였고, 처리구는 셀레늄 수준에 따라 0.1 ppm Se(대조구), 0.3 ppm Se (T1), 0.9 ppm Se (T2) 및 무기셀레늄 0.9 ppm Se (T3) 급여구로 4처리를 나누어 처리구 구당 5두씩 완전임의 배치하여 90일간 사양시험을 실시하였다. 각 시험사료는 셀레늄 강화 및 셀레늄 무처리 관행 청보리 사일리지를 조합하여 건물기준 30% 함유하도록 배합하였다. 실험사료 내에 셀레늄 강화수준은 사료섭취량과 증체에 영향을 미치지 않았고, 혈중 총 지질과 중성지방농도는 대조구보다 T2구가 유의하게 낮았다(p<0.05). 혈중 면역글로불린은 셀레늄함유량이 많을수록 높게 나타나 T2와 T3구가 대조구보다 유의하게 높았다(p<0.05). 혈중 셀레늄농도는 유기태 셀레늄을 0.9 ppm 급여한 T2구가 가장 높았다(p<0.05). 도체율, 배최장근, 등지방두께, 근내지방도, 육색 및 지방색은 셀레늄급여로 인한 효과가 나타나지 않았다. 또한, 사료 내 셀레늄 수준 및 형태는 육질 및 육량등급 간에 관련성이 형성되지 않았다. 신장, 간장 및 후지 내 셀레늄 함량은 사료 내 셀레늄 강화수준이 증가함에 따라 유의하게 직선적으로 증가하였고(p<0.05), 무기셀레늄급여는 대조구와 유의한 차이가 나타나지 않아, 조직 내 셀레늄함량 증가에 도움이 되지 않았다(p>0.05). 이상의 결과에서 셀레늄 강화 청보리 사일리지 내 존재하는 셀레늄은 조직 내 셀레늄함량을 증가시키기에 유효한 셀레늄급원인 것으로 나타났고, 셀레늄 강화 청보리 최고수준(0.9 ppm Se)으로 급여하여 생산된 한우 후지육 100 g에 함유되어 있는 셀레늄은 성인기준 1일 최소 셀레늄 권장량($40{\mu}g$)의 약 69%를 충족시키는 것으로 조사되었다.

• IMMUNE NETWORK
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• 제9권6호
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• pp.236-242
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• 2009

Background: Melanoma is the most fatal form of skin cancer due to its rapid metastasis. Recently, several studies reported that selenium can induce apoptosis in melanoma cells. However, the precise mechanism remains to be elucidated. In this study, we investigated the effect of selenium on cell proliferation in murine melanoma and on tumor growth and metastasis in C57BL/6 mice. Methods: Cell proliferation was measured by MTT assay in selenium-treated melanoma cells. Cell cycle distribution was analysized by staining DNA with propidum iodide (PI). mRNA and protein expression related to cell cycle arrest was measured by reverse transcription PCR and western blot. Tumor growth and metastasis was measured by in vivo model. Results: Selenium was suppressed the proliferation of melanoma cells in a dose dependent manner. The growth inhibition of melanoma by selenium was associated with an arrest of cell cycle distribution at G0/G1 stage. The mRNA and protein level of CDK2/CDK4 was suppressed by treatment with selenium in a time-dependent manner. In vivo, tumor growth was not suppressed by selenium; however tumor metastasis was suppressed by selenium in mouse model. Conclusion: These results suggest that selenium might be a potent agent to inhibit proliferative activity of melanoma cells.

• 원예과학기술지
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• 제19권4호
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• pp.465-470
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• 2001

토양 중 Se 함량은 논보다 밭토양에서 높았으며 지역 간 차이가 있었다. Se 함량은 Se 처리농고가 높을수록 증가였으며, 엽초보다 인경에서 많았다. 마늘에 시용한 Sodium selenate는 sodium selenite보다 Se 흡수를 많이 시켰다. Se 처리에 따른 마늘 생육은 무처리와 차이가 없었다. Sodium selenate의 엽면시용은 농도가 높아질수록 인경에서 Se 함량이 높았으나, 토양처리는 농도에 따른 차이가 뚜렷하지 않아서 엽면시용이 효율적인 것으로 보인다. 무처리구의 Se 함량은 논토양에 비하여 강우가 차단된 하우스의 인공용토에 재배한 마늘에서 높았다. 무기성분에서 인이나 유황은 인경에 많으며, 유황, 마그네슘 및 칼륨은 Se 처리구에서 무처리보다 약간 높게 나타났으나 처리농도에 따른 일정한 경향은 없었다.