• Journal of Photoscience
• /
• v.9 no.2
• /
• pp.400-402
• /
• 2002

We collected seeds of Solidago virgaurea plants growing at different altitudes on the Mt Hakusan area in Japan and cultivated them in a naturally-lit green house. Three-week-old seedlings were irradiated with supplemental UV-B for 12 h each day for 1 and 2 weeks. After a week of itradiation the seedlings of the population collected from the higher altitude at Oh-nanjiho (ON) had accumulated more anthocyanins than those from the lower altitude at Bettoh-deai (BD). Levels of anthocyanins in the ON seedlings were highly correlated with the dose of UV-B radiation and the correlation was also observed after 2 weeks. The growth of the third leaves was retarded by UV-B radiation in both populations. The extent of growth retardation in the third leaves was correlated with the dose of UV -B radiation in both populations. However, no significant difference in the extent of leaf area growth was observed between the ON and BD populations. The increase in plant fresh weight was extensively inhibited in the ON seedlings after 1 week of UV-B radiation. The inhibition was recovered to those in the BD population by 2 weeks irradiation. These results indicate that these populations respond differentially to supplementary UV -B radiation during the first week. Because flavonoids such as anthocyanins play an important role in protection against UV-B radiation in many plants, populations growing at higher altitude may be better able to adapt to increased global levels of UV-B radiation.

• Journal of Plant Biotechnology
• /
• v.4 no.3
• /
• pp.117-122
• /
• 2002

Cotton Glutathione S-Transferase(GST: EC 2.5.1.18) was cloned and Gh-5 cDNA was overexpressed in tobacco (Nicotiana tabacum) plants. The transformation of cotton GST in tobacco plant was confirmed by northern blot analysis. Type I and Type II transcript patterns were identified in Gh-5 transgenic tobacco plants. Type I transcripts was only discussed in this paper. Glutathione and 1-chloro-2,4-dinitrobenzene (CDNB) were used as the substrates, and the activity of GST in the type I transgenic plants was about 2.5-fold higher than the non-expressers and wild type tobacco plants. The expression of cotton GST in tobacco plants proved that Gh-5 could be translated into functional protein. Type I transgenic plants produced functional GST in the cells. Type I showed higher GST specific activity than Type II in the transgenic plants. Control and transgenic seedlings were grown in the growth chamber and under the light at 15$^{\circ}C$, and the effects of cotton GST in the seedlings was evaluated. The growth rate of Gh-5 overexpressors was better than the control and non-transgenic tobacco plants. Salinity tolerance was also analyzed on the seeds of transgenic plants. Seeds of Gh-5 overexpressors and the wild type tobacco seedlings were germinated and grown at 0, 50, 100, 150, and 200 mM NaCl solution. Gh-5 transgenic seedlings showed higher growth rate over control seedlings at both 50 and 100 mM NaCl solution. But at 0, 150, and 200 mM NaCl concentration, the difference in growth rate was not detected.

• Journal of Korean Society of Forest Science
• /
• v.87 no.1
• /
• pp.98-105
• /
• 1998

We measured seedling growth, foliar nutrient and extract concentrations of 3-year-old Ginkgo biloba seedlings growing in a nursery following a single fertilization with nitrogen (N), phosphorus (P) and nitrogen plus phosphorus (N+P) fertilizers. Fertilization did not change foliage, stem and root biomass of the seedlings except for the high N+P treatment, Foliar N and P concentrations following fertilization varied according to the amount of fertilizers. In general, foliar N and P concentrations increased with fertilization, but fertilization with 400kg N/ha and 100kg P/ha decreased foliar N and P concentrations, respectively. Seedling growth and foliar nutrient concentrations showed that N and P were the growth-limiting nutrients in our study site. It was found that fertilization reduced the concentrations of secondary metabolites (Ginkgo flavon glycosides and terpene lactones) in foliages. It seemed there was a relationship between foliage biomass production and secondary chemicals in G. biloba seedlings.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.47 no.5
• /
• pp.356-360
• /
• 2002

The seedlings of yacon (Polymnia sonchifolia Poeppig ＆ Endlicher), which were cuttings, plug seedling (PS), crown bud before sprouting (CBBS), crown bud after sprouting (CBAS), and divided seedling after budding (DSAB) were planted at 70$\times$50 cm planting distance on ridge; that was interow spacingintrarow spacing, and about 28,500$\pm$71 plants/ha. CBBS didn't need work and equipment to raise seedlings. PS and DSAB grew taller to 140.5 and 143.3cm, respectively, than others at 150 days after planting. In the changes of plant height, PS and DSAB showed taller than others during growth period, cuttings, CBBS, and CBAS grew rapidly in middle growth stage. Excepting main stem and petiole length, other characters were significant for seedling. Fresh weights were different among seedlings. Even though the yield of plants grown from CBAS and CBBS were lower with 34.7 and 36.4 ton/ha, respectively, than 3.6 ton/ha of DSAB; its yield index were over 95%, hence, those of plants grown from cutting and PS were lower with 73 and 87%, respectively. The ratio of tuberous roots over 200g to total tuberous roots per plant was the highest from DSAB. Most of tuberous roots were under 200g per tuberous root from cuttings. CBBS, CBAS, and DSAB are suitable to use seedlings for high yield of yacono. Yacon plant by DSAB much produced tuberous root of over 200g.

• Horticultural Science & Technology
• /
• v.35 no.1
• /
• pp.11-20
• /
• 2017

The objective of this study was to determine whether inoculation with Bacillus licheniformis MH48 as a plant growth-promoting rhizobacterium (PGPR) could promote nutrient uptake of seedlings of the ornamental plant Camellia japonica in the Saemangeum reclaimed coastal land in Korea. B. licheniformis MH48 inoculation increased total nitrogen and phosphorus content in soils by 2.2 and 20.0 fold, respectively, compared to those without bacterial inoculation. In addition, B. licheniformis MH48 produced auxin, which promoted the formation of lateral roots and root hairs, decreased production of growth-inhibiting ethylene, and alleviated salt stress. Total nitrogen and phosphorus uptake of seedlings subjected to bacterial inoculation was 2.3 and 3.6 fold higher, respectively, than the control. However, B. licheniformis MH48 inoculation had no significant effect on the growth of seedlings. Our results suggest that inoculation with B. licheniformis MH48 can be used as a PGPR bio - enhancer to stimulate fine root development, promote nutrient uptake and alleviate salt stress in ornamental plant seedlings grown in the high-salinity conditions of reclaimed coastal land.

• Plant Resources
• /
• v.4 no.1
• /
• pp.13-25
• /
• 2001

Growth and yield of licorice were investigated under the different conditions of photoperiod and shading in order to establish its cultural practice for the domestic production with the aim to substitute the import. The photoperiod was adjusted to 8,10, and 12 h by shielding plants from the light with blackout curtain. Large seedlings(11-20g) appeared to be affected by photoperiod since around 65 days. Most of growth parameters, including plant height, number of leaf, fresh and dry weight of plant and root, were the highest in 12 h photoperiod among all the photoperiod levels, excepting stem diameter which was the highest in 10 h photoperiod(4.5mm). Each photoperiod was similar to each other in root length and diameter. Small seedlings(4-l0g) showed a similar trend to large seedlings. The results from field photoperiod experiment demonstrated that 12 h photoperiod was also the best among three photoperiod treatments in plant height, stem diameter, number of leaf, root length, fresh and dry weight of plant and root. The effect of shading was tested under the three levels of control (0%), half-shading (55%), and full shading (90%). Shading remarkably suppressed the growth and yield, compared to no-shading. Although plant height and root length were little affected by the shading, stem and root diameters were heavily reduced.

• Journal of Bio-Environment Control
• /
• v.31 no.1
• /
• pp.60-66
• /
• 2022

This study was conducted to investigate growth characteristics of paprika seedlings according to various qualities of LED light (red : blue = 10 : 0, red : blue = 8 : 2, red : blue = 2 : 8, white). Plant height and stem were significantly longer or thicker as red light ratio increased. Leaf area of paprika seedlings with red light was larger or no significant differences in a mixed light of red and blue. Dry weight of seedling was in the same with the result of leaf area. Seedlings with White light was significantly less than others in all characteristics. As red light ratio was increased, relative growth rate increased. As blue light ratio was increased, the net assimilation amount increased. Considering plant height, leaf area and production ability of dry matter per unit leaf area, the using mixed red and blue lights was suitable, especially at a mixed red : blue = 8 : 2.

• Korean Journal of Environmental Agriculture
• /
• v.42 no.4
• /
• pp.382-388
• /
• 2023

A liquid-type chlorella (LC) produced by self-cultivation is used for strawberry cultivation by farmers in Korea. This study aimed to investigate seedling growth in two strawberry cultivars 'Soraya' and 'Haruhi' after applying solid-type chlorella (SC) in the greenhouse for 30 d. The treatments were as follows: Control, LC (0.2% of 1.0 × 10⁷ cells/mL), ¼ SC (12.5 g/m²), ½ SC (25 g/m²), and SC (50 g/m²). Compared to the control, in the ½ SC treatment, dry weight of the seedlings increased at 30 days after treatment (DAT). Phosphorus content in the leaves of 'Soraya' seedlings of the SC treatment increased compared to that of the control. At 10 and 30 DAT, photosynthetic pigments including chlorophylls and carotenoids in the seedlings of both cultivars increased with the ½ SC treatment. These results indicate that the application of SC improved the growth of strawberry seedlings and could replace LC.

• Journal of Ecology and Environment
• /
• v.32 no.3
• /
• pp.149-158
• /
• 2009

American sycamore seedlings were grown in chambers with two different ozone concentrations ($O_3$-free air and air with additional $O_3$) for 45 days. Both the control and the $O_3$ chambers included non-fertilized and fertilized plants. After 18 days of $O_3$ fumigation, seedlings were placed in a clean chamber for 27 days. Seedlings under ozone fumigation showed a significant decrease in pigment contents and photosynthetic activity, and a significant increase in lipid peroxidation. Fertilization enhanced physiological damage such as the inhibition of photosynthetic activity and the increase of lipid peroxidation under ozone fumigation. During the recovery phase, the physiological damage level of seedlings increased with ozone fumigation. In addition, physiological damage was observed in the fertilized seedlings. Superoxide dismutase (SOD) and glutathione reductase (GR) activities of $O_3$-treated seedlings increased up to 33.8% and 16.3% in the fertilized plants. The increase of SOD activity was higher in the fertilized plants than in the non-fertilized plants. Negative effects of ozone treatment were observed in the biomass of the leaves and the total dry weight of the fertilized sycamore seedlings. The $O_3$-treated seedlings decreased in stem, root and total dry weight, and the loss of biomass was statistically significant in the fertilized plants. In conclusion, physiological disturbance under normal nutrient conditions has an effect on growth response. In contrast, in conditions of energy shortage, although stress represents a physiological inhibition, it does not seem to affect the growth response.

• Korean Journal of Organic Agriculture
• /
• v.8 no.1
• /
• pp.89-99
• /
• 1999

To do mass multiplication of plug seedlings in potatoes, apical stem cuttings originated from virus-free microtubers were cut to one-two internodes and transplanted into the plug-tray. After 10days, we applied Chitosan and Wood Extraction on rooting and tuber formation of plug seedlings. To improve field adaptability of plug seedlings, rooted cuttings with a height of 20cm after 20days of cutting were transplanted ito the fields, We applied 500~2000ppm Chitosan on growth characteristics and tuber formation of that. The above and underground growths, such as plant height and number of leaves were significantly more vigorous after treatment with 500~1,000ppm Chitosan and 2,000ppm Wood Extraction, the spray treatment was carried out five times at intervals of four days after ten days of transplanting. T-N, K, P, Mg and Na, were higher as the concentrations of chitosan and Wood Extraction were higher. The growth and tuber yield in plug seedlings planting plot and seed potatoes planting plot were effectively highter as foliar application of Chitosan(500~2,000ppm) was done after planting the plot. T-N content in leaves and tuber was higher as the concentration of Chitosan was high. A similar tendency was shown in K, P and Mg. In the small tuber(under 30g), the number of tubers and tuber yield were relatively increased in the seed potatoes planting plot, but the large tubers(over 80g) yield was higher in the plug seedlings planting plot, and in order to increase tuber yield in plug seedlings it was necessary to add plant density to the field.