• Proceedings of the Korean Society of Plant Pathology Conference
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• 한국식물병리학회 1999년도 정기총회 및 춘계 학술발표회
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• pp.30.2-30
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• 1999

• Journal of Korean Society of Forest Science
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• 제104권1호
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• pp.60-66
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• 2015

This study was conducted to determine the effects of seed storage method ($-20^{\circ}C$, $2^{\circ}C$ dry, $2^{\circ}C$ wet 30 days, $2^{\circ}C$ wet 60 days, stratification and room temperature) and shading treatment(control, 50%, 80%) on seed germination, seedling growth of endangered species, Iris dichotoma and Iris setosa. As a result, seed germination rate of I. dichotoma was the highest at 75% when seed were stored at $2^{\circ}C$ wet 60 days and then sown under non-shading condition. The seed of I. dichotoma belong to intermediate seed. Seed germination rate of I. setosa was the highest at 95% when seed were stored at $2^{\circ}C$ wet 60 days and then sown under 80% shading condition. The seed of I. setosa belong to recalcitrant seed. Seedlings of I. dichotoma and I. setosa showed not only the best seedling quality but also seedling vigor index in seed stored at $2^{\circ}C$ wet 60 days under non-shading condition, with the growth characteristics of plant height (6.4, 7.2 cm), number of leaves (3, 4), leaf width (4.6, 3.2 mm), leaf length (5.7, 6.8 cm), fresh weight (aerial/root part; 144/260, 97/153 mg), dry weight (aerial/root; 31/20, 17/17 mg) and seedling vigor index and modified seedling vigor index (13,895/9,479, 13,256/8,668). In this research, I. dichotoma and I. setosa seed were stored at $2^{\circ}C$ wet 60 days, and then sown in non-shading condition, seed germination rate was more than 75%, 90%, respectively, and production of superior quality seedlings.

• Journal of Forest and Environmental Science
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• 제35권4호
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• pp.232-239
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• 2019

This study was carried out to establish an efficient selection condition for cold-tolerant individuals among evergreen Quercus spp. To select higher cold-tolerant individuals among four species of evergreen Quercus spp. (Q. acuta, Q. glauca, Q. myrsinaefoila, and Q. salicina), an-year-old seedlings of each species were exposed to low temperature in serial, and then examined for the death of cell tissue and the surface temperature was monitored. It was shown that the higher numbers of seedlings of Q. myrsinaefolia were survived than the others when those seedlings are exposed to cold stress. Thus, selection of the cold tolerant individuals was conducted on Q. myrsinaefolia seedlings. The limit low temperature condition for selecting cold-tolerant individuals was -6℃ for 24 hrs because no seedling of Q. myrsinaefolia was survived after exposed to -7℃ for 24 hours. It was shown that the leaf surface temperature of the selected individuals was higher than those of the non-selected individuals when they were exposed to cold-stress while monitoring them using thermal graphic camera. The results in this study can be used for expanding afforestation area of tree species of the warm temperate zone for preparation of climate change.

• Ocean and Polar Research
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• 제42권3호
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• pp.225-231
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• 2020

The aim of this study was to examine optimal induction method for carpospore release from Agarophyton vermiculophyllum cystocarps for seedling production. We tested the effects of environmental factors on carpospore release by using five different induction methods; spontaneous, desiccation, low temperature, desiccation+low temperature, and osmotic shock. Also, carpospores release was estimated at three temperatures (20, 25, and 30℃), and then under combinations of three day lengths (8, 12, and 16h) and two irradiances (30 and 60 μmol photons m^-2 s^-1), after pretreatment at desiccation+low temperature for 2 hr. The number of carpospores released was between 113 ~ 682 spores /cystocarp/day and it was maximal in the desiccation+low temperature treatment. Optimal environmental conditions for carpospore release of A. vermiculophyllum were 25℃, 16 h, and 60 μmol photons m^-2 s^-1. The present results suggest that massive carpospores for seedling production of A. vermiculophyllum could be obtained under a combination of 25℃, 16 h, and 60 μmol photons m^-2 s^-1 after pretreatment in the desiccation+low temperature.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제2권2호
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• pp.134-138
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• 1969

Experiments were carried out to asses the impotance of environmental conditions upon the growth rate, length compositions seedling and spore liberation of a red alga Glacilaric verrucosa. The alga grown in water of low salinity mixed with fresh water shows better growth rate, lager length composition and excellent seedling, whereas the one vegetated in an area of tidal exposure shows a somewhat retarded growth rate and poor seedlings. The rate of growth Increases from August to October and begins to diminish from December continuing to diminish through February. Therefore the spore settling time extends from May to lune. Although the sun-drying method is the most eacient way of inducing spore liberation, further investigations are necessary in oiler to use this method for the application to artificial spore settling.

• The Korean Journal of Ecology
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• 제17권1호
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• pp.23-35
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• 1994

To investigate phytotoxic substances in Artemisia argyi, the donor plant, and their biological activities, seed germination and seedling growth of receptor plants such as Arundinella hirta, Echinochloa crus-galli, Rumex crispus and Lactuca sativa were examined at different concentrations of aqueous extracts of the donor plant. Germination of four receptor species was inhibited by the extracts, while seedling growth was decreased to a lesser degree than in the germintion test. Germination, seedling growth and dry weight growth of Achyranthes japonica grown in pot were proportionally inhibited by the extracts. Volatile substances emitted from A, argi plant caused slight inhibition in the germination and seedling growth of the receptor species. Essential oil of the plant extracted by Karlsruker's apparatus inhibited growth of microorganisms and callus growth of Pinellia ternata and Oryza sativa. The GC /MS method was employed for analysis and identification of allelochemicals from A. argyi leaves. Sixty-one chemical substances such as a-pinene, camphene, 1. 8-cineol, etc. were identified from essential oil of A. argyi. The results of this experiment on seed germination, seedling growth, microorganism culture and tissue culture indicated that naturally occurring chemical substances from A. argyi would be responsible for the growth inhibition of plants studied.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제35권4호
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• pp.320-327
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• 1990

Experiments were conducted to investigate the method to raise seedling establishment rate and the optimum seeding dates and rates in direct seeding culture using transplanter under submerged field condition in Chuncheon area during 1986 to 1988. Direct seeding on submerged field using transplanting machine after sowing on seedling growing box contained with paddy or upland soil was possible. Higher rate of seedling establishment was obtained in case of sowing sprouted seed and intermittent irrigation after sowing. Seedling establishment rate was higher both in April 30 or May 10 seeding plot. Minimum daily average air and soil temperature to ensure more than 70 percent seedling establishment was 14.8$^{\circ}C$ and 16.3$^{\circ}C$ respectively. Optimum sowing date in the direct sowing was early May and optimum sowing rate was 200g per box, 6kg per l0a. In this case rice grain yield was 481kg per l0a and this is almost same yield level compared hand and machine transplanting.

• Journal of Bio-Environment Control
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• 제32권3호
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• pp.210-216
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• 2023

Conventionally, the seeds of Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. have been directly sown at the field without using the plug seedling method. Plug seedlings have the advantage of promoting germination and convenient transplanting. However, there is little information about propagation of R. glutinosa using the plug seedling method. This study was conducted to investigate the optimal seed rhizome length, diameter, and sowing direction of R. glutinosa for establishing the plug seedling method. Seed rhizome length and diameter were separated by 1, 2, 3 cm and 0.3-0.5, 0.6-1.0, 1.1-1.5 cm, respectively. And seed rhizomes were sown in vertical and horizontal directions. The survival rate in 1 cm length of seed rhizomes was lower than in other treatments. The leaf length, leaf width, number of leaves, SPAD, leaf area, and fresh and dry weights of shoot and roots were the greatest in 3 cm length of seed rhizomes. As the seed rhizome diameter decreased, the growth characteristics of R. glutinosa tended to increase. When R. glutinosa was sown horizontal direction, the leaf length, leaf width, number of leaves, and leaf area were significantly higher than in the vertical direction. In conclusion, when sowing seed rhizome in a plug tray, using a length of 3 cm, a diameter of less than 1 cm, and sowing in a horizontal direction is considered an appropriate sowing method for R. glutinosa.

• Journal of The Korean Society of Grassland and Forage Science
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• 제11권3호
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• pp.145-152
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• 1991

This experiment was carried out to investigate the effects of liming, manure application, and inoculation with Rhizobium on the early growth, nodulation, seedling establishment, winter survival, and forage yield and quality of alfalfa(Medicago sativa L.) at the Experimental Livestock Farm, College of Agriculture, Seoul National University, Suweon in 1985 and 1986. The results obtained are as follows: 1. Seedling vigor and nodule formation of alfalfa at early stage were the best in the treatments of liminginoculation( L1) and liming-manure-inoculation(LM1). 2. Seedling establishment was not significantly affected by the treatments of liming, manure application, nodulation and the combination of these. 3. The LI and LMI treatments showed the highest winter survival of 94.3 and 83.5%, respectively, but the lowest winter su.rvival of 55.9 and 58.5% was found in no treatment(None) and inoculation( I ), respectively. 4. No differences in crude protein(CP1, acid detergent fiber(ADF) and neutral detergent fiber(NDF) contents were found among the treatments. 5. There were significant differences in the total dry matter yield of alfalfa among the treatments. The total dry matter yields of 10,618, 11,830, 11,855 and 13,456 kg/ha from the treatments of L, LM, LI and LMI, respectively, were significantly higher than those of None, M and I treatments. 6. Based on the results of the experiment, it appears that the dry matter yield of alfalfa could be enhanced by the treatments of liming with Rhizobium inoculation and also liming, manure application and Rhizobium inoculation as a practical method.

• Korean Journal of Medicinal Crop Science
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• 제20권5호
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• pp.365-371
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• 2012

This study was carried out to know the characteristics of flowering and bearing fruit, the optimum period, regions and methods for seed harvesting, the optimum temperatures for seed storage and germination, and the optimum period for sowing at nursery bed and seedling transplanting of Valeriana fauriei Briquet. The flowering and bearing fruit of Valeriana fauriei was developed from the before-year root. Optimum period for seed harvest of Valeriana fauriei was from late July to middle August, and optimum areas were the high elevated areas over 500 m above the sea level as Jinbu-myeon, Pyeongchang-gun, Gangwon-do. Using of net-bag for seed harvesting was the effective method to gather the full ripe seed, and bagging of net-bag was necessary from the season of middle May that was the flowering middle-stage. Germination rates don't show the difference among the different temperatures of storage as approximately 41% at $-20^{\circ}C$, $2^{\circ}C$ or $20^{\circ}C$ of seed storage temperatures. The optimum temperature range was in $15{\sim}30^{\circ}C$ for seed germination at nursery bed. The optimum period for seed sowing at nursery bed was the late February, and the optimum period for seedling transplanting was the middle April.