• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.26 no.2
• /
• pp.192-197
• /
• 1981

Alfalfa (Medicago sativa L. cv. Luna) seeded in agar was inoculated with two strains of Rhizobium meliloti isolated from root nodules of alfalfa for assessment of nodulation. The seedling growth after six weeks was remarkably increased by adding each rhizobia strains into agar media and also by nitrate application (70ug N/ml), but there was no significant difference among them. Nodulations started one week after inoculation and increased its numbers and sizes as seedling grew. Therefore, the two strains isolated from alfalfa root were concluded to be effective strains. For determining seed inoculation method the same cultivar was inoculated with both rhizobia strains using different inoculation methods such as broth-vacuum, peat-adhesive, peat & lime pelleting. They were seeded in pots of river sand and supplied with culture solution excluded nitrogen. The peat & lime pelleting was recognized the best method in both of nodulation and seedling growth after eight weeks growth. There were significant correlations between the weight of nodules and the shoot or root dry weight of alfalfa in both rhizobia strains.

• ALGAE
• /
• v.37 no.2
• /
• pp.105-121
• /
• 2022

Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.41 no.6
• /
• pp.698-703
• /
• 1996

To elucidate the proper nitrogen application method for machine transplanting of rice infant seedling in no-tilled paddy, Dongjinbyeo was used and nitrogen was applied five methods including conventional application method. Leaf color was darker at 80% of total nitrogen was applied as basal and lighter at 20% of total nitrogen was reduced than conventional application method in tillering stage, but wasn't significantly different after panicle formation stage among the application method. And it was thinner at tillering stage and was darker after panicle formation stage in no-tilled paddy compared with tilled paddy. Tiller number, LAI and top dry weight were more at 80% of total nitrogen was ap-plied as basal than conventional application method, and more in tilled paddy than no-tilled one at tillering stage, but at heading stage they were highest at top dressing applied 25 days after transplanting. C.G.R was highest at top dressing for tillering was delayed and it was lower in no-tilled paddy at tillering stage but was higher after panicle formation stage than in tilled one. Yield was highest at top dressing time was delayed among nitrogen application method, but wasn't significantly different between tilled method in same nitrogen application method.

• Journal of Korean Society of Forest Science
• /
• v.86 no.4
• /
• pp.443-449
• /
• 1997

We examined the effect of pretreatments on seed germination, seedling growth, optimal seedling density of Phellodendron amurense. The seeds were carefully purified after collection in late Oct. from natural population at Mt. Chiri. For the evaluation of pretreatment effects, seeds were sowed in an experimental nursery after being stored in open ground($2{\sim}8^{\circ}C$) with soaking in 20% $H_2O_2$, 50-fold pon-pon solution, cold moist stratification in refrigerator($2{\sim}4^{\circ}C$) and dry storage in roam temperature($4{\sim}18^{\circ}C$), respectively, For the effect of growth density of seedlings, seedlings germinated in nursery were precisely controlled with 53, 104, 220, 380 seedlings per $m^2$. Among pretreatments for the promotion of germination rate, pon-pon treatment(68.8%) was highest, the others were higher than that in dry storage(2.3%), and these seed pretreatment methods were significantly different at 1% level. The height growth of seedlings showed the most vigor from Jun.12 to Jul. 8, and from Jul.20 to Aug. 2, and it reached to 72.8% of height growth. In case of 1-0 seedling, although seedling density per $m^2$ was increased, seedling growth was decreased. The optimal seedling density was 104 seedlings per $m^2$. The standard seedlings for plantation were above height 55cm, root collar diameter 8.1mm, and root length 21cm. These results demonstrated that seed of P. amurense requires stratification for the germination promotion.

• The Korean Journal of Ecology
• /
• v.27 no.5
• /
• pp.307-310
• /
• 2004

It was found that seed germination and seedling growth of selected species were inhibited by phytotoxic substance released from fir trees. The aqueous extracts of leaves caused significant inhibition in the seed germination of the receptor plants, Whereas seed germination of some species was not inhibited in extracts of leaves, but seedling elongation of the receptor plants was also decreased by the aqueous extract. Dry weight growth was slightly increased in lower concentration of the extract, while that was proportionally inhibited by higher concentration of the extracts. Chemical substances of fir trees were shown the biological toxicity. The GC method was employed for analysis of phytotoxic chemicals and sixteen chemical substances were identified such as benzoic, phenylacetic, etc. Also 33 volatile substances were identified from the fir tree. These chemical compounds are assumed the substances related to allelopathic potential of Abies holophylla plant species.

• Mycobiology
• /
• v.28 no.4
• /
• pp.180-184
• /
• 2000

In order to find an environment-friendly method to suppress astragal stem rot caused by the isolates of Rhizoctonia solani AG 1 and AG 4, we tested an antagonistic fungus Gliocladium virens G1 was evaluated as a biocontrol agent and estimated inorganic compounds and organic materials were tested for their effect of the disease suppression. G. virens G1 effectively inhibited mycelial growth in a dual culture and caused mycelial lysis in the culture filtrate. No adverse effect was observed when examined for seed germination and seedling growth. Promoted seedling growth was observed with the seed treatment. Seeds of astragal plant were germinated higher in the sterile soil than the natural soil. Of 14 inorganics tested, alum, aluminum sulfate and calcium oxide significantly suppressed the mycelial growth and sclerotial germination. Milled pine bark and oak sawdust also suppressed the mycelial growth. Soil amended with 1% of G. virens G1 composted with pine bark (w/v) significantly controlled astragal stem rot in the glasshouse experiments.

• Journal of Species Research
• /
• v.6 no.1
• /
• pp.68-75
• /
• 2017

We investigated the effects of sodium hypochlorite (NaOCl) and culture medium on embryo swelling and germination of Calanthe discolor Lindl., and established a method for determining the swelling and protocorm formation of C. discolor seeds via in vitro examination of immature seeds. Treatment of immature seeds with NaOCl greatly enhanced the extent of embryo swelling and protocorm formation of immature zygote embryos compared to seeds without NaOCl treatment. The effects of the culture media were also evaluated with regard to embryo swelling and protocorm formation of in vitro cultured seeds with and without NaOCl treatment. Additionally, the effects of white fluorescent light and red and blue LEDs lights on seedling growth in in vitro culture were examined. The most suitable condition for seedling growth after 12 weeks of culture was the red LEDs light with POM medium. These results show effective asymbiotic germination and growth of C. discolor.

• Korean Journal of Food Science and Technology
• /
• v.34 no.1
• /
• pp.128-131
• /
• 2002

'Manita', a red pepper cultivar being cultivated currently and 'HL', a cultivar bred for single-harvest by National Horticultural Research Institute were grown by direct sowing and raising seedling methods, respectively, and all the fruits on the tree were harvested once. Fruits were grouped into 6 grades by color and appearance and their chemical compositions were analyzed. Generally, 'HL' showed less redness and contained less amounts of capsaicin, organic acid, Vit. C, and sugar than 'Manita'. Regardless of cultivar and cultivation method, red-old fruits, ripened and partially dried on the plant, had more red color (21-30%) and more capsaicin (40.0-78.3 mg% vs. 33.2-52.7 mg%), but less sugar (12.70-16.69% vs. 14.46-17.43%) than red-fresh fruits. No difference was found between direct sowing and raising seedling.

• Mycobiology
• /
• v.30 no.1
• /
• pp.47-50
• /
• 2002

Seed-borne fungi of some maize cultivars/lines grown during the months from May to September of 2001, collected from Dongguk University farm, Go Young City, IL Sang Gu, Korea were detected by blotter method. In all six fungi namely Alternaria alternata(Fr.) Keissler, Aspergillus niger Van Tiegh, Fusarium moniliforme Sheldon, Fusarium sp., Penicillium sp. and Ustilago zeae Unger. were found to associated with maize seeds. Prevalence of seed-borne fungi also varied. The highest percentages of seed-borne fungi were recorded with Fusarium moniliforme and the lowest in Penicillium sp. Transmission of all seed-borne pathogens from seeds to seedlings were also detected by test tube seedling symptom test. Among the seed-borne fungi, Alternaria alternata, Fusarium moniliforme and Fusarium sp. produced distinct seed rot and seedling infection symptoms. All the transmitted seed-borne fungi might be caused primary source of infection to the maize crop.

• Korean Journal of Organic Agriculture
• /
• v.19 no.spc
• /
• pp.135-138
• /
• 2011

In traditional organic rice culture practices, control of weeds is a big problem. This study was conducted to increase the rice production. Results showed that Plant height, SPAD, root length and weight were higher in pot raised seedling than broadcasting method except number of panicles. High plant density caused reduction in plant height, number of culm and chlorophyll content of the rice. No lodging was observed in both methods. Thickness of third internode and culm length was more in pot raised seedlings at both sites. When organic material was used rice yield increased by 3.81%. Higher rice production (10%) was recorded in the fields planted with pot raised seedlings. From the above study it could be conclude that the pot raised seedlings perform better than the seedlings raised by broadcasting methods.