• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.24-24
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• 2017

Heavy metals at toxic levels have the capability to interact with several vital cellular biomolecules such as nuclear proteins and DNA, leading to oxidative stress in plants. The present study was performed to explore the metal tolerance mechanism in Sorghum seedling. Morpho-physiological and metal ions uptake changes were observed prominently in the seedlings when the plants were subjected to different concentrations of $CuSO_4$ and $CdCl_2$. The observed morphological changes revealed that the plants treated with Cu and Cd displayed dramatically altered shoot lengths, fresh weights, and relative water content. In addition, the concentration of Cu and Cd was markedly increased by treatment with Cu and Cd, and the amount of interacting ions taken up by the shoots and roots was significantly and directly correlated with the applied level of Cu and Cd. Using the 2-DE method, a total of 24 and 21 differentially expressed protein spots from sorghum leaves and roots respectively, 33 protein spots from sorghum leaves under Cd stress were analyzed using MALDI-TOF/TOF MS. However, the over-expression of GAPDH plays a significant role in assisting Sorghum bicolor to attenuate the adverse effects of oxidative stress caused by Cu, and the proteins involved in resistance to stress helped the sorghum plants to tolerate high levels of Cu. Significant changes were absorbed in the levels of proteins known to be involved in carbohydrate metabolism, transcriptional regulation, translation and stress responses. In addition, the up-regulation of glutathione S-transferase and cytochrome P450 may play a significant role in Cd-related toxicity and stress responses. The results obtained from the present study may provide insights into the tolerance mechanism of seedling leaves and roots in Sorghum under heavy metal stress.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.104-104
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• 2017

Sorghum (Sorghum bicolor (L.) Moench.) is one of the most important crops for human and animal nutrition. Nonetheless, sorghum has a cyanogenic glucoside compound which can be degraded into hydrogen cyanide, toxic to humans and animals even with tiny amount. In consequence, breeding materials with a low cyanide level has been a top priority in sorghum breeding programs. To fulfill our long-term goal, we are screening sorghum accessions with low cyanide level, which would be an important breeding material for food safety. We collected seeds of various sorghum accessions and analyzed relevant metabolites to find useful breeding materials of sorghum accessions containing low cyanide. Fourteen wild relatives were obtained from the University of Georgia in US, a reference accession BTx623, and three local varieties from National Agrobiodiversity Center of Rural Development Administration in Korea, and one wild species from the Wild Plant Resources Seed Bank of Korea University in Korea. Sorghum plants were grown in plastic greenhouse under natural conditions. After growing, leaf samples were harvested at different developmental stages: seedling phase, vegetative phase (right before flowering), and reproductive phase (ripening). Using collected samples, quantification analysis were performed by an HPLC system for three metabolites (dhurrin, 4-hydroxybenzaldehyde, and 4-hydroxyphenylacetic acid) in sorghum plants. Prior to metabolome analysis, specific experimental condition for HPLC system was set to be able to separate three metabolites simultaneously. Under this condition, these metabolites were quantified in each accession by HPLC system. We observed that the metabolite contents were changed differently by developmental stages and accessions. We clustered these results into five groups as patterns of their contents by developmental stages. Most of accessions showed that 4-hydroxybenzaldehyde content was very high at seedling stage and decreased rapidly at vegetative phase. Interestingly, the patterns of dhurrin content were very different among clusters. However, 4-hydroxyphenylacetic acid content was maintained at low levels by developmental stages in most accessions. The results would demonstrate how dhurrin and alternative degradation pathways are differentiated in each accession.

• Journal of Korean Society of Forest Science
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• v.88 no.4
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• pp.469-476
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• 1999

This study was carried out to investigate the growth and physiological response of shade intolerant and intermediate tree seedlings to enhanced UV-$B_{BE}$(biologically effective UV-B) radiation. The seedlings of Betula platyphylla var. japonica Hara(shade intolerant species) and Fraxinus rhynchophylla Hance(shade intermediate species) were treated with one of the three levels of UV-$B_{BE}$ dosages - ambient UV-$B_{BE}$, ambient+3.2, and ambient+$5.2\;KJ\;m^{-2}day^{-1}$) for 14 weeks in the field condition. Height and root collar diameter growth, leaf area, and biomass production of the seedlings of two species were reduced by enhanced UV-B radiation. Leaf stomatal resistance to water vapor of the F. rhynchophylla seedling was increased by the UV-B increment. The reductions in total chlorophyll and carotenoid were more apparent in the F. rhynchophylla than B. platyphylla seedling. There was no statistically significant changes in the concentration of UV-B absorbing compound($A_{300}$) in the leaves of the two species among the UV-B treatment. However the $A_{300}$ tented to be increased in F. rhynchophylla by enhanced UV-B radiation. These results indicate that the growth and the physiological and biochemical responses between B. platyphylla and F. rhynchophylla were different to enhanced UV-B environment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.341-349
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• 2014

Field emergence of Azuki bean is poor due to hard seed coat as compared to other legumes. In this study, an attempt was made to develop prediction method with regression analysis based on various seed vigor tests in laboratory for field emergence of azuki bean. Azuki bean seeds artificially aged to provide various levels of seed quality were evaluated by the standard germination test (SGT), cold germination test (CT), cool germination test (CGT), complex stressing vigor test (CSVT), tetrazolium(TZ) vigor test and electroconductivity test. The SGT was suitable for predicting the field emergence in the unaged high vigor seeds. The abnormal seedling percentage and shoot length in the CGT were highly correlated with field emergence of moderate vigor seeds artificially aged for 2 days. Electroconductivity, seed viability in the CSVT, and vigor and predicted germinability in the tetrazolium vigor test were also useful for predicting field emergence. Percent of ungerminated seed in the CSVT was correlated with field emergence in the low vigor seeds artificially aged for 4 days. In a stepwise multiple regression analysis, seed viability in the SGT, normal seedling percentage and dry matter weight in the CGT accounted for 86.9% of the predicted value of field emergence in azuki bean.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.2
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• pp.126-133
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• 1988

To know the optimum harvest time for seed yield and seed quality a local variety of rye 'Paldang-homil'was harvested at seven different harvest times from 25 to 55 day after heading (DAH) at five-day intervals in 1984 ani 1986. Seed development, seed germination and seedling growth were observed. The l000-grain weight increased as harvest time delayed until 50 DAH in both years. Although grain yield tended to increase with delay of harvest time, the yield differences between succeding harvest time was highest between 40 DAH and 45 DAH. Germination rate of seeds harvested before 30 DAH were lower than those after 35 DAH at 20 C, but at 10 and 30 C before 35 DAH were lower after 40 DAH. Plant height and dry weight of seedlings increased with delay of harvest time up to 45 DAH in pot. Heading stages were similar among the seeds harvested 40-55 DAH. Culm length was not different among the harvest times. The optimum harvest time for seed production of rye seems to be 45 DAH (38 days after flowering).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.198-208
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• 1992

Recently, a nursery mat made from rock wool has realized transplanting of the younger seedlings with the ordinary transplanting machines for Chibyo and Chubyo(3 and 4～5 leaved seedling, respectively). The seedlings defined as the 'Nyubyo' or 'Nursling seedlings' became possible to achieve economic profits from the reduction in both working time and costs. It being widely noticed as a strategy to solve the difficulties in current rice cultivation. The nursling seedlings are 1.4 to 2.5 leaves and height at 4.5 to 7cm, grown 4 to 7 days after seeding. They maintain still up to 50 to 80% of their nutrients in the endosperm, and can grow by using only their own nutrients for a certain period of time after transplanting. Nursling seedlings take 2 days in the nursery chamber at 32$^{\circ}C$ after seeding, and 2 days in the greening house at $25^{\circ}C$. This is only 4 days, all together, to make the nursling seedlings of 1.5 leaves which are ready for transplanting. Watering is only needed once at the sowing time. It only takes 1 or 2 waterings even to raise a seedlings for a period of 7 days. The number of nursery boxes can be reduced because it is possible to sow more densely(220 to 240g per box), thus it only needs seedlings of 15 to 16 boxes per 10 a which leads to a reduction in facilities and space needed. Temperature during the nursery period can be artificially adjusted more precisely which may lead to the prevention of temperature stress. The nursling seedlings can root rapid by because the crown roots from the coleoptile node begin to emerge immediately after transplanting. They show strong resistance to low temperature (12$^{\circ}C$) and deep-planting. There is no danger in the rooting of the seedlings even if half of their height is buried into the soil. Moreover, it can root at a rate of up to 65 to 80% even if the full height of the seedlings is buried. They show also strong resistance to submergence (10～15cm). The nursling seedlings tend to grow by producing tillers from lower nodes. It is therefore, necessary to control to keep the proper numbers of tillers per unit area. They have no fear in the delay of heading and their yield components can be so well balanced that the same level of yield was achieved with the nursling seedlings compared to that with Chibyo. It was further suggested that if the surplus tillers can be avoided by such cultivation practices, the number of grain per panicle can be kept greater and higher yield can be realized. Practical experiments with the nursling seedlings conducted in 1989 and 1990 by farmers in various areas showed exciting results. The nursling seedlings will become widely spread, or at least occupy an important position in Japanese and also in Korean rice cultivation techniques.tivation techniques.

• Korean Journal of Environment and Ecology
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• v.17 no.4
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• pp.337-345
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• 2004

The objective of this study was to monitor vegetation recovery process after timber harvesting at (Mt.) Baekwoonsan Seoul National University Forests, Korea. Two monitoring plots were established in 1994 and woody plant were monitored in 1997, 1999, 2001, and 2003. Vegetation development pattern during last ten years (1994-2003) after timber harvesting were as follows; Styrax obassia, Styrax japonica and Lindera erythrocarpa as of the existing tree were competitive species in the first year after clear-cut, Styrax japonica and Lindera erythrocarpa as of sprout tree and Aralia elata as of seedling were dominant species in the sixth year after clear-cut, and Lindera erythrocarpa, Styrax japonica and Quercus serrata were dominant species from the eighth year to the tenth year after clear-cut. Species diversity index of harvested forest interior was decreased at the southwestern slope while it was increased in the northeastern slope till 6th year and decreased after the 8th year). According to DBH distribution pattern, No. of individuals of Quercus serrata, Styrax japonica and Lindera erythrocarpa showed high frequency in the southwestern slope, and Acer pseudosieboldianum, Styrax obassia, Magnolia sieboldii, Lindera erythrocarpa, and Aralia elata showed good growth in the northeastern slope. There was a difference between slopes in Basal area. It was decreased at the southwestern slopes during the 10th year continuously and it was increased the sixth year however, was decreased after the eighth year at the northeastern slope.

• Journal of Ginseng Research
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• v.33 no.2
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• pp.127-131
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• 2009

The plant protection rate of the marketed microbial products for ginseng cultivation was investigated against Rhizoctoina solani and Pythium sp. in a seedling pot experiment. A significant difference was found among the mean plant protection rates (Pm) of the microbial products, including Tolclofos-methyl (Rhizolex). The best microbial product, C-ISR2, showed a 33% and 33.6% net Pm (total Pm-control Pm) in the two tests against Rhizoctonia solani. In one test with a 58.6% control plot Pm, the total pm was 91.6%, indicating that plant protection can be done only with a microbial product in a well-conditioned field. The net Pm of C-ISR2 against Pythium sp. was 26.4%. The net Pm of a microbial product against a pathogen seems to be fixed. A significant negative linear correlation was found between the Pm and the coefficient of variation (CV) of the protection rate in all the three experiments. This indicates that the protection processes of control, microbial products, and chemical pesticides are in the same system. Pm was only dependent on CV, probably due to each seedling's microenvironment. In the linear correlation equation between the Pm and the CV of the microbial products and the control plot, the intercept of the vertical axis will be the theoretical Pm when CV is zero.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.598-607
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• 2016

This study was conducted to evaluate the amount of nitrogen (N) top dressing based on the normalized difference vegetation indices (NDVI) by ground based sensors for leaf perilla under the polyethylene house. Experimental design was the randomized complete block design for five N fertilization levels and conventional fertilization with 3 and 4 replications in Gumsan-gun and Milyang-si field, respectively. Dry weight (DW), concentration of N, and amount of N uptake by leaf perilla as well as NDVIs from sensors were measured monthly. Difference of growth characteristics among treatments in Gumsan field was wider than Milyang. SPAD-502 chlorophyll meter reading explained 43.4% of the variability in N content of leaves in Gumsan field at $150^{th}$ day after seedling (DAS) and 45.9% in Milyang at $239^{th}$ DAS. Indexes of red sensor (RNDVI) and amber sensor (ANDVI) at $172^{th}$ day after seedling (DAS) in Gumsan explained 50% and 57% of the variability in N content of leaves. RNDVI and ANDVI at $31^{th}$ DAS in Milyang explained 60% and 65% of the variability in DW of leaves. Based on the relationship between ANDVI and N application rate, ANDVI at $172^{th}$ DAS in Gumsan explained 57% of the variability in N application rate but non significant relationship in Milyang field. Average sufficiency index (SI) calculated from ratio of each measurement index per maximum index of ANDVI at $172^{th}$ DAS in Gumsan explained 73% of the variability in N application rate. Although the relationship between NDVIs and growth characteristics was various upon growing season, SI by NDVIs of ground based remote sensors at top dressing season was thought to be useful index for recommendation of N top dressing rate of leaf perilla.

• Journal of Bio-Environment Control
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• v.6 no.1
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• pp.15-25
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• 1997

Plug seedlings of bell pepper(Capsicum annuum L.) were grown for 50 days in controlled environment chambers under 12 hrs per day photoperiodic condition with sixteen different day and night temperature regimes to investigate the possibility of height control. The seedlings were then transplanted to greenhouse to investigate the growth, flowering, and yield afterward. Plant height and stem length of seedlings were mainly affected by day temperature rather than night temperature. Internode elongation was suppressed by a negative DIF and was enhanced by a positive DIF even with the same average daily temperature (ADT). Leaf unfolding rate was influenced more by ADT than by DIF. Fresh and dry weights increased as ADT increased. Leaf area and stem diameter increased until temperature increased up to 24$^{\circ}C$ day and night temperature and decreased above 24$^{\circ}C$, The position at which the first flower was initiated was lowered as ADT increased. The first flower degeneration was not obvious up to 24$^{\circ}C$ ADT but increased rapidly above 24$^{\circ}C$ ADT. Seedling compactness(Dry weight per plant height :mg.mm$^{-1}$ ) was greater under -DIF than +DIF condition. In conclusion, DIF treatment was an applicable technique to control stem elongation and growth rate such as leaf unfolding rate and position at which first flower was initiated could be controlled by ADT.