Park, Dong-Bae;Myung, Jae-Woo;Na, Bong-Kwon;Kang, Chan Hyoung
Journal of the Korean institute of surface engineering
/
v.46
no.4
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pp.145-152
/
2013
The growth behavior of nanocrystalline diamond (NCD) film has been studied for three different substrates, i.e. bare Si wafer, 1 ${\mu}m$ thick W and Ti films deposited on Si wafer by DC sputter. The surface roughness values of the substrates measured by AFM were Si < W < Ti. After ultrasonic seeding treatment using nanometer sized diamond powder, surface roughness remained as Si < W < Ti. The contact angles of the substrates were Si ($56^{\circ}$) > W ($31^{\circ}$) > Ti ($0^{\circ}$). During deposition in the microwave plasma CVD system, NCD particles were formed and evolved to film. For the first 0.5h, the values of NCD particle density were measured as Si < W < Ti. Since the energy barrier for heterogeneous nucleation is proportional to the contact angle of the substrate, the initial nucleus or particle densities are believed to be Si < W < Ti. Meanwhile, the NCD growth rate up to 2 h was W > Si > Ti. In the case of W substrate, NCD particles were coalesced and evolved to the film in the short time of 0.5 h, which could be attributed to the fact that the diffusion of carbon species on W substrate was fast. The slower diffusion of carbon on Si substrate is believed to be the reason for slower film growth than on W substrate. The surface of Ti substrate was observed as a vertically aligned needle shape. The NCD particle formed on the top of a Ti needle should be coalesced with the particle on the nearby needle by carbon diffusion. In this case, the diffusion length is longer than that of Si or W substrate which shows a relatively flat surface. This results in a slow growth rate of NCD on Ti substrate. As deposition time is prolonged, NCD particles grow with carbon species attached from the plasma and coalesce with nearby particles, leaving many voids in NCD/Ti interface. The low adhesion of NCD films on Ti substrate is related to the void structure of NCD/Ti interface.
In order to study the biolgiocal effects of dry back following EMS (Ethyl methane sulfonate) treatment on seeds of 'Dunghan Shali' that belongs to indica-type rice with various presoaking(24, 36, and 40 hrs respectivily) and treatment time(l, 2 and 3 hrs respectivily), this experiment was done. Seedling heights of wet seeds presoaked for 36 hrs was only greatly decreased with EMS treatment compared with other periods. In germination rate of wet seeds, all of these damage was not changed. For the drying back seeds, otherwise, seedling height and germination rate were greatly reduced depend upon the time of treatment and presoaking as well as storage effects after drying back. Reduction patterns, both seedling height and germination rate, between 1 week and 8 weeks after dry back were quite similar. The other experiment was carried out in order to study for the influences on the seedling growth as biological damage with EMS and MMS(Methyl methane sulfonate) treatments among different varieties. The indica-type rice varieties; Taichung Native No.1, Dunghan Shali and IR-8, were used as the experimental materials. From this trial some results were obtained as follows; 1. Both seedling growth and germination rate on all varieties, used, MMS, treatment showed more toxic effects than those of EMS treatment. 2. Seeding growth injury of rice seeds dried back was increased gradually, and then was approached the maximum at 6-8 days after storing. In IR-s variety, otherwise, growth damage was appeared a little. Germination rate of these were quite smilar, even though chemical used is different and storage period was prolonged. 3. It was showed clearly that varietal differences of chemical mutagene treatments were recognized.
Kim, Hye Min;Park, Jin Young;Kim, Eun Young;Song, Jeong Eun;Kwon, Soon Yong;Chung, Jin Wha;Khang, Gilson
Polymer(Korea)
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v.38
no.3
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pp.278-285
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2014
Demineralized bone particles (DBP) and alginate hybrid sponges were fabricated at 10, 20, 40 and 80% DBP/alginate hybrid ratios for seeding chondrocyte. Cell proliferation was measured via MTT assay. Morphological observation, histology, biological assay and RT-PCR were performed at each time point 1, 2 and 3 weeks. The cell viability was better in 20% DBP/alginate sponges than in other sponges. SEM results showed that more attached and more proliferated cells in the 20% DBP/alginate sponges with the lapse of time. Finally, histochemical assay results showed that the phenotype of chondrocyte was well maintained and both acidic mucopolysaccharide and type II collagen was well formed at 20% sponges. This study suggested that DBP/alginate sponge may serve as a potential cell delivery vehicle and a structural basis for tissue engineered articular cartilage.
This study was performed to evaluate the effects of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) on the characteristics of beagle dog's periodontal ligament (BPD) cells and bone marrow (BBM) cells which have the important role on the early stage of periodontal tissue regeneration in vitro. In control group, the cells ($1.5{\times}10^5$cells/ml) were cultured alone with Dulbecco's Modified Eagle's Medium contained with 10% fetal bovine serum, $50{\mu]g/ml$ ascorbic acid, and 10mM/ml ${\beta}-glycerophosphate$. In experimental groups, growth factors, PDGF or EGF(10ng/ml), were added into the above culture condition. And then each group was characterized by examining the cell proliferation rate, amount of total protein synthesis, alkaline phosphatase activity at 1, 5, 9, 13, 17th day after seeding of cells into the culture wells. The results were as follows: 1. Both BPD and BBM cells in PDGF-treated group proliferated more rapidly than non-treated cells. This finding also was observed in EGF-treated group but it was not as prominent as that of PDGF-treated group. The proliferation rates of both cells showed the time-dependent pattern during experimental periods in all three groups. 2. Amount of total protein synthesis was more increased in PDGF-treated group than in control group. But no significant difference between EGF-treated group and control group was observed throughout experimental periods even though the tendency of amount of protein synthesis was time-dependent pattern. 3. Alkaline phosphatase activity also more increased in PDGF-treated group than control group. But slight decrease tendency was seen in both cells of EGF-treated group. From the above results, PDGF appeared to enhance the proliferation and cellular activities including amount of total protein synthesis and alkaline phosphatase activity of BPD and BBM cell, but EGF did not show notable effects. The optimal application of these growth factors was thought to be useful as the adjunctive means in periodontal regeneration procedures.
The study was conducted to obtain the basic information on the diseases epidemics of vegetable crops grown in Namji, Jinju, Gimhae and Suncheon under the extremely varied and specified conditions, plastic film house. The disease survey was conducted from the end of April, 1951 to April 1982. Leaf mold and late blight were serious foliar disease in tomato during the seedling stage, especially when the infected seeds were sown. The diseases increased rapidly 35 days after seeding. In both continuous cultivation of cucumber and rotation with upland crops, incidence of Fusarium wilt was severe while incidence of the disease was negligible in cultivations after paddy rice or grafting on pumpkin. Downy mildew of cucumber was severe in Jinju and Suncheon area, however, it was not so serious in Namji area where the growing season of cucumber was unfavorable for the maximum disease incidence. Cucumber mosaic virus disease was prevalent in the areas surveyed and the disease incidence was increased rapidly after June. Powdery mildew prevailed at the early stage of cucumber growth after transplanting in Namji area. Root rot and wilting caused by Phytophthora capsici was as the most destructive disease in pepper grown under the vinyl house, especially in Namji and Jinju area where the pepper has been cultivated intensively. The Phytophthora attacked most parts of young plants during the winter time and then induced crown rot on the adults plants. Cultivation of pepper in vinyl house was almost impossible because of the Phytophora disease by the end of June. Virus diseases to tomato plants were prevalent throughout the surveyed area and the damage was also severe. In Jinju and Gimhae area leaf mold and late blight showed high infection rate in tomato during the harvest time.
Weeding measures is an important issue for cereal crop because weed is superior to crops in early growth stage and there are not many herbicides applicable to cereal crop. Since the concern about well-being and eco-environmental products are increasing, cultivation with polyvinyl chloride (PVC) is needed. Five treatments included as weed-free, control (not remove weed) and covering with polyvinyl chloride removed 20, 30, 40 DAS (day after seeding). At removing PVC, plant height did not differ depending on the growth stage. However, weed-free plot by weed competition were somewhat short. And, removing PVC accelerated the earlier by 2-3 day than weed-free plot. Soil moisture which removed from the control was higher than 20, 30, 40 DAS and weed-free, and it was kept low by evaporation. The chlorophyll meter of weed-free plot was 34.3, 30 DAS was 40.6 at heading time. Weed-free showed that increased in weed population $300.8g\;m^{-2}$. Weed control value of 20 DAS, 30 DAS, 40 DAS were 86.5%, 84.0% and 74.8%, respectively. In 30 DAS, yield of foxtail millet according to treatment of removing PVC were $355kg\;10a^{-1}$. At weed competition early in 30 DAS, the removing PVC was good for control. Thus, 30 DAS was judged to be suitable.
Proceedings of the Korean Society of Crop Science Conference
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2022.10a
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pp.52-52
/
2022
Recently, PE (polyethylene) film has been used increasingly in com cultivation. However, PE films often cause soil and environment contamination. In order to reduce this problem, many researches have been carrying out studies on biodegradable films (BF) that are easily decomposed by soil microorganisms. Therefore, this study was conducted to determine which BF is optimal for growth and yield of com crops while also having the highest rates of film decomposition. BFs Farmsbio (Farm Hannong), Heulgro Film (Sejin Bio), Vonto Film (Eco-Hansung) as well as a selected PE film were used in this study. For the control, we used crops grown without any kind of mulching. Experimental fields were fertilized according to conventional cultivation methods, tilled, and then covered by either BF or PE. After 1 week, com (cv. MIBECK2ho) at the 3-leaf stage (16 days after seeding) was transplanted. Plant height was measured at 18 and 32 days after transplanting and heading stages. Yield components and yield were also measured at harvest. In addition, pH, EC, and decomposition and light transmittance levels of films were investigated during the experimental period. Daily average temperature, relative humidity and organic matter in soils were also measured during the experimental period. There was no significant difference in plant height, heading date, and silking between crops with BFs and PE, but the crops grown with BFs and PE films reached higher growth parameters in a shorter amount of time than the crops in the non-mulching control. Additionally, there were no significant differences in yield components such as length of ears, ear width, ear weight, and yield in crops that were grown using films or crops in the control plot. Light transmittance and decomposition levels of films generally increased with time after transplanting, and was highest in the Heulgro film than other BFs. Soil pH and organic matter in crops using BFs and PE films were significantly higher than in the control plot at 99 and 113 days after transplanting. In general, the EC contents in the control plot was lower than in crops using BFs and PE films. The average daily moisture in soil was higher when BFs and PE films were used than in the control plot. However, the daily average soil temperature was higher in crops using BFs and PE films than in the control plots at the beginning of the experimental period, but there was no consistent difference in soil temperature towards the later part of the experimental period. Therefore, the BFs used in this study were shown to be helpful without causing negative impacts on the growth and yield of com.
To measure the soil-to-plant transfer factors ($TF_a,\;m^2\;kg^{-1}$-fresh) of radionuclides deposited during the growing season of potato, a radioactive solution containing $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ was applied to the soil surfaces in soil boxes 2 d before seeding and three different times during the plant growth. For the pre-seeding application (PSA), radionuclides were mixed with the topsoil (loamy sand and 5.2 in pH). The plant parts investigated were leaves, stems, tuber skin and tuber flesh. The $TF_a$ values of $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ from the PSA were in the ranges of $1.9{\times}10^{-4}{\sim}1.5{\times}10^{-2}$, $1.8{\times}10^{-4}{\sim}7.5{\times}10^{-4}$, $4.0{\times}10^{-4}{\sim}1.6{\times}10^{-2}$, $1.5{\times}10^{-4}{\sim}3.9{\times}10^{-4}$ respectively, for different plant parts. The TFa values from the growing-time applications were on the whole a few times lower than those from the PSA. For $^{54}Mn,\;^{85}Sr$ and $^{137}Cs$, the $TF_a$ values from the early- or middle-growth-stage application were higher than those from the late-growth-stage application, whereas the opposite was true for $^{60}Co$. Leaves and tuber flesh had the highest and lowest $TF_a$ values, respectively, in most cases. The total uptake from soil by the four plant parts was in the range of $0.05{\sim}3.16%$. In the third year following the PSA, the $TF_a$ values of $^{54}Mn,\;^{60}Co$ and $^{137}Cs$ were $11{\sim}25%$, $21{\sim}25%$ and $38{\sim}67%$ of those in the first year, respectively, depending on the plant parts. The present results can be used for estimating the radiological impact of an acute radioactive deposition during the growing season of potato and for testing the validity of relevant food-chain models.
Cultivation of winter cover crops and its soil utilization for summer main crops in slope upland is very important at the respects of diminution of soil erosion and non-point pollution source. The effects of cover crop, hairy vetch as no-tillage or conventional tillage on prevention of soil erosion and reduction of chemical nitrogen fertilization were investigated in the slope upland with whiter clover living mulch (partial tillage) in Suwon for three years and with rye (conventional tillage) in Hongcheon for two years, respectively. In Suwon, amounts of soil lost by rainfall runoff decreased as much as 90% by hairy vetch-no tillage (HV-NT), white clover-partial tillage (WF-PT) together with the decrease of rainfall runoff compared to winter fallow with conventional tillage (WF-CT). In addition, amounts of weed also decreased as much as 80-90% by HV-NT and WF-PT. Corn yield decreased much at the plot of WF-PT mainly due to competition for soil water and nutrients between clover and corn at the early corn growth stage. On the contrary, corn yield increased by HV-NT compared to WF-CT regardless of weed control. In Hongcheon, amounts of soil eroded during winter season before corn seeding were reduced as much as 95% by cultivation of hairy vetch and rye compared to winter fallow. Amount of soil eroded during waxy corn growing season was reduced as much as 98% by HV-NT compared to WF-CT. Also, soil incorporation of hairy vetch and rye as green manure with conventional tillage at corn seeding time could reduce soil erosion as much as 70% compared to no soil cover with conventional tillage. Ear yields of waxy corn were increased 10% higher at hairy vetch green manure (HV-CT) without nitrogen fertilizer, 20% higher at HV-NT with standard nitrogen fertilizer, respectively than WF-CT. But ear yields of waxy corn were decreased by rye green manure (R-CT) and HV-NT at the condition of no nitrogen fertilizer. It was concluded that hairy vetch was better as winter cover crop to reduce both soil erosion and chemical nitrogen fertilizer simultaneously in slope upland than other cover crops.
Background: The purpose of this study is to improve the quality of the diagnostic procedures in the preoperative evaluation so as to reduce the unnecessary thoracotomy and to ensure resectability in non-small cell lung cancer. Material and Method: Of 616 patients who underwent thoracotomy for primary lung cancer from January 1990 to December 1996, 59 patients(9.6%) turned out to have inoperable lesions after the thoracotomy. We reprospectively reviewed the bronchoscopic findings, methods of tissue diagnosis, CT scans, pulmonary function test and lung perfusion scan, reasons for nonresectability, and adjuvant therapy, and then followed up on the survival rate after exploratory thoracotomy. Result: The cell types were squamous cell carcinoma in 38, adenocarcinoma in 15, large cell carcinoma in 3 and others in 3. Primary loci were RUL in 20, RML in 6, RLL in 8, LUL in 13, LLL in 4 and others in 8. The reasons for non-resectability were various; direct tumor invaison to mediastinal structures(n=41), seeding on pleural cavity(n=8), poor pulmonary function(n=2), invasions to extranodal mediastinal lymph node(n=2), technical non- resectability due to extensive chest wall invasion (n=3), small cell carcinoma (n=1), malignant lymphoma(n=1), and multiple rib metastases(n=1). In the follow-up of 58 patients, 1-year survival rate was 55.2% and 2-year survival rate was 17.2% and the mean survival time was 14 months. When compared according to cell types or postoperative adjuvant therapeutic modalities, no significant difference in the survival rates were found. The squamous cell carcinoma was frequently accompanied by local extension to contiguous structures and was the main cause of non-resectability. In adenocarcinoma, pleural seeding with malignant effusion was frequently encountered, and was the major reason for non-resectability. Conclusion: These data revealed that if appropriate preoperative diagnostic tools had been available, many unnecessary thoracotomies could have been avoided. Both the use of thoracoscopy in selected cases of adenocarcinoma and the more aggressive surgical approach to the locally advanced tumor could reduce the incidence of unnecessary thoracotomies for non-small cell lung cancers.
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