• Journal of Forest and Environmental Science
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• 제37권4호
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• pp.309-314
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• 2021

This study aimed to enhance seed productivity and secure genetic resources for Pinus densiflora for. multicaulis. We analyzed the characteristics of cone and seed generated by control pollination between Pinus densiflora (PD) and Pinus densiflora for. multicaulis (PDM). The highest number of cone scales (63.0) was obtained from the self-pollinated (sp) PDM clone B (PDM-sp-B), whereas the lowest number of cone scales (44.7) was obtained from two combinations designated as PDM-A×PD-075 and PDM-A×PD-0111. Both female parents of the hybrids were PDM-A. The highest seed production capacity (80.8) was obtained from the open-pollinated (op) PDM clone B (PDM-op-B). The seed potentials of PDM-B×PD-0111, PDM-op-A, and PDM-sp-B were 67.4, 66.5, and 63.1, respectively. The highest number of fertile scales (41.5) was obtained from PDM-op-B, and the lowest number of fertile scales (28.8) was obtained from PDM-A×PD-075. The total number of aborted ovules and 1st aborted ovules was not statistically significant in the mating design. The cross combination of PDM-B×PD-0111 had the highest number (34.8) of filled seeds and the lowest number of 2nd aborted ovules (5.2) and empty seeds (9). PDM-op-B had the highest number of developed seeds (47.6), although the number of empty seeds was the highest (41.2). Therefore, we conclude that the mating design of PDM-B×PD-0111 is useful for future breeding programs to improve seed yield of PDM. Our results showed that there was a strong correlation between the following two parameter pairs: number of scales and number of fertile scales, and the number of fertility scales and seeds potential (r=0.89 and r=0.84, respectively; both p<0.01).

• 한국양식학회지
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• 제10권3호
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• pp.373-380
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• 1997

순환 여과 시스템에서 나일틸라피아의 종묘생산성을 향상시키기 위한 연구의 일환으로 암컷 친어의 크기, 친어 방양 관리 방법에 따른 생산성과 생산된 난의 인공부화 성공률을 조사하였다. 암컷 친어의 크기가 작고 (평균 어체중 : 340.0g) 방양 밀도가 낮을 때(1.2 kg m 상(-2))의 종묘 생산량이 크기가 크고 (평균 어체중: 611.5g) 방양 밀도가 높을 때 (2.1kg m 상(-2) 보다 유의하게 많은 것으로 조사되었다(P<0.05). 대량 종묘 생산에 있어서 구중 부화 중인 난과 난황 자어의 제거후 인공 부화를 통한 인공부화법 (clutch removal method)은 유영 자어의 수집에 의한 자연부화법 (natural mouthbrooding method)에 비해 총생산량, 단위 면적당 생산량, 암컷 마리당 생산량 및 암컷 단위 어체중당 생산량에서 유의하게 높은 것으로 조사되었다(P<0.05). 암컷 친어의 구강으로부터 제거한 알의 발생 단계별 인공 부화 실시 결과 55.7%-91.6%의 부화율을 보였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제18권2호
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• pp.83-89
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• 2009

Multiplication and spore production of three microsporidia(Nosema bombycis, Nosema sp. 1 and Nosema sp. 2) in selected tissues of pupa and adult of silkworm, Bombyx mori L. were studied in two seasons (SI, SII) with distinct temperature (SI: $20.1{\pm}0.8^{\circ}C$ and SII: $25.1{\pm}0.7^{\circ}C$) regimes. Multiplication of the microsporidia followed a logistic pattern with a lag phase, an exponential phase and a stationary phase. In SII, spore production was significantly (P<0.01) higher in various tissues. Highest spore production was observed 30 days post inoculation (p.i.) in SI and in SII, it was $21{\sim}23$ days p.i. Spore production was significantly (P<0.01) higher in the gut tissues than other tissues. Nosema sp. 2 registered significantly (P<0.01) higher spore production in both the seasons compared to Nosema bombycis and Nosema sp. 1. Results indicate that the multiplication and spore production of microsporidia are tissue specific and extremely sensitive to the temperature at which the host is reared. Through this study, the precise day that the spore numbers of the microsporidia are maximized can be predicted in both pupa and adult in case the infection is initiated in the first instar.

• Journal of Ginseng Research
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• 제32권2호
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• pp.155-160
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• 2008

The North American ginseng (Panax quinquefolius L.) seed crop varies from year to year. The ability to hold stratified seed for a year would ensure continuity of seed supply and no interruption in production cycles. Seed drying and rehydration protocols at room temperature $(21{\pm}2^{\circ}C)$ were developed. These protocols and seed storage at 4 ${\pm}1^{\circ}C$ and 35%, or variable, relative humidity (RH) allowed the holding of stratified seed for one year and then establishment of the following five treatments in field plots: Trt.1 : dried 2005 stratified seed (seed harvested Fall 2004) held at $4^{\circ}C$ and at variable humidity; Trt.2 : 2006 stratified seed planted directly into the field; Trt.3 : 2005 stratified seed dried in October 2005 and held at $4^{\circ}C$ and 35% RH ; Trt.4 : 2005 stratified seed held in moist sand from October to December 2005 at room temperature $(21{\pm}2^{\circ}C)$ and then in December dried and held at $4^{\circ}C$ and 35 % RH; Trt.5 : 2005 stratified seed held in moist sand from October to December 2005 at room temperature and then in December dried and held at $-12^{\circ}C$ Seedling emergence was best in Trts. 2 and 4 with 67.3 and 65.1% respectively which is similar to the industry expected rate of 68% after regular stratification. Seedling growth was similar in Trts. 2 and 4 with root dry weights of 172 and 159 mg respectively in mid-August. Therefore, if holding stratified seed in August/September for one year is desired, the seed can be placed in moist sand until December and then dried and stored at $4^{\circ}C$ and 35% RH. These seed can be planted in the following August/September and will germinate and grow in the following year to give an acceptable crop.

• 한국환경과학회지
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• 제24권12호
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• pp.1681-1689
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• 2015

This study was conducted to investigate days after anthesis (DAA) and fruit after-ripening period (FAP) for seed-harvesting of high quality watermelon seeds. Fruit weight and number of seed per fruit increased according to DAA, while those did not significant about FAP. Ratio of cotyledon at whole seed was higher about 2 to 4% compared to seed coat irrespective of DAA and FAP. Germinability of watermelon was not a significant effect by DAA, however, it had differences by FAP. Percent of germination (PB) was below 50%, when 30 days maturated fruits after anthesis was omitted ripening, while PB was increased to 92% by ripening. In addition, seeds at DAA 40 and FAP 20 were higher general seedling vigors (hypocotyl length, diameter etc.) in BP test. Results indicated that considering seed productivity, it had maximized seed viability at DAA 40 and FAP 20.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.223-223
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• 2022

Saengcheonggukjang, known as Natto in Japan, is a Korean fermented soybean food that has various bioactive compounds for antioxidant and antidiabetic activity. The development of soybean (Glycine max L.) cultivars for saengcheonggukjang production relies on the selection of seed traits that influence the quality and sensory properties. One of the important traits for cultivars is seed characteristics such as seed hardness and size. In order to select the lines for breeding good quality saengcheonggukjang varieties, several simple sequence repeat (SSR) markers and quantitative trait loci (QTLs) related to seed quality of Korean cultivars, Pungsannamulkong, Socheongja, Pungwon, Heawon, and Hoseo, were analyzed. Based on the many studies to detect stable QTLs for seed traits, we tested several QTLs related to seed size and water absorbability using SSR markers on Korean cultivars. The results showed that two regions for water absorbability of Pungsannamulkong and one region for seed size traits of Haewon and Hoseo were identified in this study. These results could have applications to soybean breeding for seed size and hardness and it is necessary to narrow it down through further study.

• 한국작물학회지
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• 제29권1호
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• pp.62-66
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• 1984

옥수수 변형단교잡종 채종재배에 있어서 웅주간파 ♂♀ 1 : 2, 2 : 4 등의 파종양식과 재식밀도가 종자친의 생육 및 채종량과 화분친의 웅수특성에 미치는 영향을 구명하고자 1983년 제천옥의 종자친 KS8/B68과 화분친 KS5를 공시하여 시험한 결과를 요약하면 다음과 같다. 1. 개화기 및 간장은 파종양식이나 재식밀도에 크게 영향을 받지 않았고 종자친의 착수고는 파종양식간에는 차이가 없었으나 재식밀도가 증가함에 따라 직선적으로 증가하였다. 2. 종자친의 100주당 수수는 10a당 종자친 3,500본에서는 파종양식간에 비슷하나 5,000본 이상에서는 2 : 4 재배보다 웅주간파나 1 : 2재배에서 많았으며 재식밀도 증가에 따라 파종양식에 관계없이 직선적으로 감소하였다. 수당입수는 파종양식간에는 차이가 없고 파종양식과 재식밀도의 상호작용도 유의하지 않았으나 재식밀도 증가에 따라 작아졌다. 100입중은 25g 내외로 처리간 차이가 없었다. 3. 화분친의 화분이 제한되지 않은 조건에서의 채종량은 웅주간파, 1 : 2재배, 2 : 4재배순으로 많은 경향이였고 10a당 종자 친 3,500본에서 5,000본으로 증가시킴에 따라 크게 증가하나 5,000본에서 6,500본으로 재식밀도 증가에 따른 채종량 증가는 크지 않았다. 4. 화분친의 웅수장과 웅수당 영화수는 1 : 2나 2 :4재배에 비하여 웅주간파 재배에서 작았고 재식밀도 증가에 따라 1 : 2 및 2 : 4재배에서는 크게 감소하였으나 2: 4재배에서는 유의하게 작아지지 않았다. 5. 변형단교잡종의 채종에는 1 : 2나 2 : 4재배의 10a당 종자친 5,000본 재식이 적합할 것으로 생각된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1993년도 추계 학술대회지
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• pp.50-51
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• 1993

• 한국작물학회:학술대회논문집
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• 한국작물학회 1993년도 추계 학술대회지
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• pp.48-49
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• 1993

• 한국육종학회지
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• 제40권3호
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• pp.263-268
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• 2008

잣나무 채종원의 유전간벌을 위하여 재적생장에 대한 개량 효과와 유효클론수를 이용한 유전다양성을 평가하였다. 유전 간벌은 클론의 일반조합능력으로 나타나는 육종가(breeding value), 암꽃과 수꽃 개화량을 이용한 종자생산 기여도, 클론별 ramet수 등를 이용한 클론별 변이를 기초로 하였다. 차대 검정 결과에 의하여 산출된 재적생장에 대한 클론별 일반조합능력(genetic value) 및 1991년부터 2003년까지 조사된 개화량 성적을 이용하여 잣나무 채종원(1981년 조성) 179클론에 대하여 간벌 강도에 따른 유전다양성 및 개량효과를 분석하였다. 이때 채종원 외부로부터의 화분오염율은 30%(gene migration = 15%)로 설정하였다. 그 결과 재적생장에 대한 개량효과는 간벌율이 10%에서 60%까지 높아짐에 따라서 점차 증가하였다. 하지만 이보다 더 높은 간벌 강도에서 현저한 개량효과 증가는 나타나지 않았다. 유효클론수는 간벌강도 10%에서 60% 사이에서는 40클론으로 나타났으며, 70% 간벌율 이상에서는 증가하거나 감소하는 변동을 나타냈다. 따라서 본 잣나무 채종원에 대해서는 50%에서 60% 수준의 간벌 강도가 적당한 것으로 판단된다.