• Title/Summary/Keyword: seed industry

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A TMT-based quantitative proteomic analysis provides insights into the protein changes in the seeds of high- and low- protein content soybean cultivars

  • Min, Cheol Woo;Gupta, Ravi;Truong, Nguyen Van;Bae, Jin Woo;Ko, Jong Min;Lee, Byong Won;Kim, Sun Tae
    • Journal of Plant Biotechnology
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    • v.47 no.3
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    • pp.209-217
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    • 2020
  • The presence of high amounts of seed storage proteins (SSPs) improves the overall quality of soybean seeds. However, these SSPs pose a major limitation due to their high abundance in soybean seeds. Although various technical advancements including mass-spectrometry and bioinformatics resources were reported, only limited information has been derived to date on soybean seeds at proteome level. Here, we applied a tandem mass tags (TMT)-based quantitative proteomic analysis to identify the significantly modulated proteins in the seeds of two soybean cultivars showing varying protein contents. This approach led to the identification of 5,678 proteins of which 13 and 1,133 proteins showed significant changes in Daewon (low-protein content cultivar) and Saedanbaek (high-protein content cultivar) respectively. Functional annotation revealed that proteins with increased abundance in Saedanbaek were mainly associated with the amino acid and protein metabolism involved in protein synthesis, folding, targeting, and degradation. Taken together, the results presented here provide a pipeline for soybean seed proteome analysis and contribute a better understanding of proteomic changes that may lead to alteration in the protein contents in soybean seeds.

A Case Study on the ODA for Creating Shared Value (CSV) in Agricultural Sector Based on the Value Chain Analysis - Project for Establishment of Seed-Potato Production System in Vietnam - (가치사슬분석법에 기초한 농업분야 공유가치창출(CSV) ODA 사례분석 - 베트남 씨감자 생산체계 구축사업 -)

  • Ji, Seong-Tae
    • Journal of Agricultural Extension & Community Development
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    • v.25 no.1
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    • pp.31-44
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    • 2018
  • This is a case study on "the Project for Production Facility and Technical Support of Processed Seed-Potato (2008-2010)" based on the Value Chain Analysis (VCA) used to create and plan International development cooperation projects. The project is the earliest model of Creating Shared Value (CSV) in the agricultural sector. For the case analysis, a framework was established to assess the effectiveness and impact of the CSV project based on the main factors of the VCA. As a result of the assessment, project participation by OSI was able to increase the overall utility by developing the Vietnamese potato processing industry and meeting consumer demand. Furthermore, it formed a business model to promote win-win cooperation and upgraded the value chain of the potato industry. In addition, it contributed to the improvement of incomes and the quality of life of farmers and communities by providing technical guidance and purchase of contracts, as well as labor division and cooperation with other activity supporters.

Study on non-destructive sorting technique for lettuce(Lactuca sativa L) seed using fourier transform near-Infrared spectrometer (FT-NIR을 이용한 상추(Lactuca sativa L) 종자의 비파괴 선별 기술에 관한 연구)

  • Ahn, Chi-Kook;Cho, Byoung-Kwan;Kang, Jum-Soon;Lee, Kang-Jin
    • Korean Journal of Agricultural Science
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    • v.39 no.1
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    • pp.111-116
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    • 2012
  • Nondestructive evaluation of seed viability is one of the highly demanding technologies for seed production industry. Conventional seed sorting technologies, such as tetrazolium and standard germination test are destructive, time consuming, and labor intensive methods. Near infrared spectroscopy technique has shown good potential for nondestructive quality measurements for food and agricultural products. In this study, FT-NIR spectroscopy was used to classify normal and artificially aged lettuce seeds. The spectra with the range of 1100~2500 nm were scanned for lettuce seeds and analyzed using the principal component analysis(PCA) method. To classify viable seeds from nonviable seeds, a calibration modeling set was developed with a partial least square(PLS) method. The calibration model developed from PLS resulted in 98% classification accuracy with the Savitzky-Golay $1^{st}$ derivative preprocessing method. The prediction accuracy for the test data set was 93% with the MSC(Multiplicative Scatter Correction) preprocessing method. The results show that FT-NIR has good potential for discriminating non-viable lettuce seeds from viable ones.

Low-Temperature Storage of Immature (Green) North American Ginseng Seed for Fall Planting

  • Proctor John T.A.;Louttit Dean
    • Journal of Ginseng Research
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    • v.30 no.2
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    • pp.78-81
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    • 2006
  • Freshly harvested, immature (green) seeds of North American ginseng (Panax quinquefolius L.) were stratified for up to 3 years in plastic pails in controlled environment rooms at $5{\pm}1^{\circ}C$ for 9 months and then $21{\pm}2^{\circ}C$ for 3 months (Trt. 1, regular stratification), or continuously at $-2{\pm}0.2^{\circ}C$ (Trt. 2), or continuously at $3{\pm}0.2^{\circ}C$ (Trt. 3). During stratification at -2 and $3^{\circ}C$ embryos did not grow. On seeding in the field embryos grew rapidly and resultant seedlings were comparable to those from regularly stratified seed. Seedling emergence rate was acceptable at the industry expected rate of 68% after one year of storage, but not after two years storage when it declined to 17.5%. Seed rot was so severe in year 3 that no planting was carried out. Seedling and second year growth were similar at the three stratification temperatures; most importantly, root dry weight (economic yield) was similar. Low-temperature storage of freshly-harvested North American ginseng seed is an acceptable method for short-term retention of propagating material.

Development of Multipurpose Seed Paper from Waste Paper ( I ) - Focused on functional chemicals behavior - (폐지를 이용한 기능성 육묘지의 제조(제1보) -기능성 약제의 거동-)

  • Park Soung Bae;Eom Tae Jin
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.36 no.4 s.107
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    • pp.41-48
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    • 2004
  • To make a basepaper for multipurposed seed paper, old news print (ONP) and mixed office waste paper (MOW), modified and unmodified with a commercial cellulase, were investigated. Each handsheet was applied with different chemicals such as insecticides, germicides and herbicides. The interactions behaviors of chemicals used with base papers were evaluated by means of the contents of chemicals impregnation and dissolving behaviors in water and soil. The ONP and MOW treated with the cellulase had higher impregnation and dissolving capacities in both water and soil than untreated ONP and MOW. However, the modified ONP showed lower impregnation and dissolving capacities compared to the modified MOW. The content of impregnation of chemicals would be affected with the degree of microfibrils produced by the modification treatment. Otherwise, dissolving capacities of chemicals depend on the affinity of the residual lignin in the paper.

Rheological Properties of White Pan Bread Dough Prepared with Lotus (Nelumbo nucifera) Seeds Powder (연자육 분말을 첨가한 식빵 반죽의 물리적 특성)

  • Lee, Byung-Gu;Byun, Gwang-In
    • Food Science and Preservation
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    • v.15 no.6
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    • pp.852-858
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    • 2008
  • This study investigated the rheological properties of bread dough supplemented with lotus (Nelumbo nucifera) seed powder. The rheological properties measured were dough volume, farinogram, amylogram, extensogram, pH and outernal. The lotus seed powder contained 7.74% moisture, 20.15% crude protein, 2.11% crude fat, 4.34% crude ash, and 2.78% crude fiber. The farinogram showed that with increasing concentration of lotus seed powder the absorption rate of the dough increased slightly, the development time and stability decreased, and the degree of attenuation tended to be grown along. From the amylogram it was found that the gelatinization onset temperature and the maximum viscosity of the dough tended to increase with increasing content of lotus seed powder. The extensogram showed that the degree of extension of the dough decreased with increasing content of lotus seed powder, while the degree of resistance and resistance/extensibility increased. The dough pH tended to decrease with fermentation time, but increased with increasing content of lotus seed powder. A concentration of $5{\sim}10%$ lotus seed powder appears to be suitable for the preparation of dough.

Development of an Economic Assessment Model for the Selection of Indoor Air Pollutant Low Emission Material for G-SEED (G-SEED용 실내공기 오염물질 저방출 자재 선정을 위한 경제성 평가 모델 개발)

  • Kwon, Seong-Min;Kim, Byung-Soo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.41 no.3
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    • pp.289-296
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    • 2021
  • The Korean construction industry has been implementing G-SEED, a certification system that evaluates the environmental properties of buildings for the purpose of reducing environmental burdens such as energy and resource consumption and pollutant emissions. Also, creating a pleasant environment in general is one more purpose of G-SEED certification system. However, G-SEED certification in practice is difficult and time consuming due to the complexity of the certification acquisition process coupled with little economic consideration for the materials of each certification item. Therefore, in this study, we present a model for the optimal selection of materials and economic assessment using a genetic algorithm. The development of the model involves building a material database based on life-cycle costing (LCC) targeted at "Application of Indoor Air Pollutant Low Emission Material" from G-SEED. Next, the model was validated using a real non-residential building case study. The result shows an average cost reduction rate of 74.5 % compared with the existing cost. This model is expected to be used as an economically efficient tool in G-SEED.

Determination and Prediction of the Amino Acid Digestibility of Sunflower Seed Meals in Growing Pigs

  • Liu, J.D.;Li, Q.Y.;Zeng, Z.K.;Li, P.;Xu, X.;Wang, H.L.;Zhang, S.;Piao, X.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.1
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    • pp.86-94
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    • 2015
  • This experiment was conducted to evaluate the chemical composition and amino acid (AA) digestibility of sunflower seed meal (SFSM) and to use this data to develop prediction equations for estimating AA digestibility for growing pigs. Ten SFSM were collected from five provinces in China. Twelve barrows ($38.8{\pm}4.6kg$), fitted with ileal T-cannula were allotted into two $6{\times}6$ Latin square designs. Each of six experimental periods comprised a 5-d adaption period followed by a 2-d collection of ileal digesta. The ten test diets contained 50% SFSM as the sole source of AA. Another nitrogen-free diet was used to measure the basal endogenous losses of crude protein (CP) and AA. Chromic oxide (0.3%) was used as an inert marker in each diet. There was considerable variation (CV>10%) among the ten SFSM in chemical composition (dry matter [DM]). The concentration of CP and ether extract (EE) ranged from 29.33% to 39.09% and 0.88% to 11.33%, respectively. Crude fibre (CF), neutral detergent fibre and acid detergent fibre ranged from 21.46% to 36.42%, 38.15% to 55.40%, and 24.59% to 37.34%, respectively. There was variation among the ten SFSM in apparent ileal digestibility (AID) and standardized ileal digestibility (SID) for lysine and threonine, which ranged from 63.16 to 79.21 and 55.19% to 72.04% for AID and 67.03% to 82.07% and 61.97% to 77.01% for SID, respectively. The variation in CP and methionine ranged from 60.13% to 74.72% and 74.79% to 88.60% for AID and 66.70% to 79.31% and 77.16% to 90.27% for SID, respectively. Methionine was a good indicator to predict AA digestibility. These results indicate that conventional chemical composition of SFSM was variable (CV>10%) among the ten SFSM (DM). The results of AID, SID and prediction equations could be used to evaluate the digestibility of SFSM in growing pigs.

Analysis of reactive species in water activated by plasma and application to seed germination

  • Choi, Ki-Hong;Lee, Han-Ju;Park, Gyungsoon;Choi, Eun-Ha
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.162.1-162.1
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    • 2015
  • The use of plasma has increased in bio-application field in recent years. Particularly, water treated by arc discharge or atmospheric pressure plasma has been actively utilized in bio-industry. In this study, we have developed a plasma activated water generating system. For this system, two kinds of plasma sources; dielectric barrier discharge (DBD) plasma and arc discharge plasma have been used. The discharge energy was calculated using the breakdown voltage and current, and the emission spectrum was measured to investigate the generated reactive species. We also analyzed the amount of reactive oxygen and nitrogen species in water using the chemical methods and nitric oxide sensor. Finally, the influence of plasma generated reactive species on the germination and growth of spinach (Spinacia oleracea) was investigated. Spinach is a green leafy vegetable that contains a large amount of various physiologically active organic compounds. However, it is characterized with a low seed germination rate.

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