• Title/Summary/Keyword: seed germination test

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Factors Involved in Promoting Seed Germination of Foeniculum vulgare (회향종자의 발아촉진에 관여하는 요인)

  • Eun Il, Lee;Chu Ho, Choi;Jong Ki, Lee;Seok Hyeon, Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.4
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    • pp.435-445
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    • 1997
  • The effects of temperature, prechilling, chemicals such as $GA_3$, IAA, kinetin and $KNO_3$ on the germinability of Foeniculum vulgare seed of medicinal plant were examined. In Foeniculum vulgare, the germination rate appeared to be around 50~60% or more in general, showing no differences in germination rate with different temperatures, promoting substances, physical or chemical treatments, and prechilling treatments. The observation of embryo under stereoscopic microscope for Foeniculum vulgare in Umbelliferae showed that seeds with or without embryo was almost the same in number. This result suggests that the lower rate of germination in this species is caused by embryolessness of seeds. The straight-shaped embryos as well as Y-shaped embryos were also observed. Foeniculum vulgare of medicinal plants in Umbelliferae were observed under scanning electron microscope, and did not show any opening problem near micropyle area. Final count should be made on 7th day of germination test.

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Changes of Fatty Acid during Germination by Seed Pretreatment, SMP, in Tobacco (SMP종자 전처리에 의한 담배 종자 발아과정의 지방산 변화)

  • Shin Ju-Sik;Kim Young-Sin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.3
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    • pp.156-160
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    • 2005
  • We studied the effect of SMP (solid matrix priming) treatment, seed pretreatment for germination enhancement, to tobacco seeds by measurement of germination rate and contents of fatty acid, energy source for tobacco germination. The results were as follows. In result of germination test, germinative ability was the highest in KF109 at nine day treatment and in KB108 at seven day treatment. The composition of fatty acid in tobacco seed confirmed by gas chromatography were palmitic acid, stearic acid, oleic acid+elaidic acid, linoleic acid+linole­laidic acid, and $\alpha-linolenic$ acid. Palmitic acid, stearic acid, oleic acid and elaidic acid, and linoleic acid and linole-laidic acid were highest in KF109 at eight or nine day treatment and in KB108 at six or seven day treatment. Especially, content of oleic acid + elaidic acid, and linoleic acid+linolelaidic acid were changed largely by treatment, so these might be used for index to examine treatment effect.

Germination Prediction of Cucumber (cucumis sativus) Seed using Raman Spectroscopy (라만분광을 이용한 오이 종자의 발아예측)

  • Mo, Changyeun;Kang, Sukwon;Lee, Kangjin;Kim, Giyoung;Cho, Byoung-Kwan;Lim, Jong-Guk;Lee, Ho-Sun;Park, Jongryul
    • Journal of Biosystems Engineering
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    • v.37 no.6
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    • pp.404-410
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    • 2012
  • Purpose: The objective of this research was to select high quality cucumber (cucumis sativus) seed by classifying into viable or non-viable one using Raman spectroscopy. Method: Both transmission and back-scattering Raman spectra of viable and non-viable seeds in the range from $150cm^{-1}$ to $1890cm^{-1}$ were collected with a laser illumination. Results: The Raman spectra of cucumber seed showed Raman peaks with features of polyunsaturated fatty acids. The partial least squares-discriminant analysis (PLS-DA) to predict viable seeds was developed with measured transmission and backscattering spectra with Raman spectroscopy and germination test results. Various types of spectra pretreatment were investigated to develop the classification models. The results of developed PLS-DA models using the transmission spectra with mean normalization or range normalization, and back-scattering spectra with mean normalization treatment or baseline correction showed 100% discrimination accuracy. Conclusions: These results showed that Raman spectroscopy technologies can be used to select the high quality cucumber seeds.

Study on non-destructive sorting technique for lettuce(Lactuca sativa L) seed using fourier transform near-Infrared spectrometer (FT-NIR을 이용한 상추(Lactuca sativa L) 종자의 비파괴 선별 기술에 관한 연구)

  • Ahn, Chi-Kook;Cho, Byoung-Kwan;Kang, Jum-Soon;Lee, Kang-Jin
    • Korean Journal of Agricultural Science
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    • v.39 no.1
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    • pp.111-116
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    • 2012
  • Nondestructive evaluation of seed viability is one of the highly demanding technologies for seed production industry. Conventional seed sorting technologies, such as tetrazolium and standard germination test are destructive, time consuming, and labor intensive methods. Near infrared spectroscopy technique has shown good potential for nondestructive quality measurements for food and agricultural products. In this study, FT-NIR spectroscopy was used to classify normal and artificially aged lettuce seeds. The spectra with the range of 1100~2500 nm were scanned for lettuce seeds and analyzed using the principal component analysis(PCA) method. To classify viable seeds from nonviable seeds, a calibration modeling set was developed with a partial least square(PLS) method. The calibration model developed from PLS resulted in 98% classification accuracy with the Savitzky-Golay $1^{st}$ derivative preprocessing method. The prediction accuracy for the test data set was 93% with the MSC(Multiplicative Scatter Correction) preprocessing method. The results show that FT-NIR has good potential for discriminating non-viable lettuce seeds from viable ones.

Germination and Physical Characteristics of Pelleted Sesame Seed with Different Materials (참깨종자의 과위화 재료에 따른 발아 및 물리적 특성)

  • 오명규;김종태;유숙종;고종철;박문수;이중용
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.5
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    • pp.497-502
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    • 1997
  • A seed pelleting technique was developed for easy handling of small sesame seeds (Chinbaeckkae) and for direct seeding in field. The mixture of pelleting material, binder(sodium alginate) and seeds were dropped in 100mM CaCl$_2$ solution. Charcoal was appeared as a best material among all to make pellets with the characters of hardness, breakdown, destruction rate and germination percentage. Zeolite formed the hardest pellet seeds among the materials and 1~3 seeds contained in a pellet on the treatment of 87g of sesame seeds mixed in 1$\ell$ CaCl$_2$ solution was estimated optimum rate of seed mixure with few appearance of seedless pellet. The moisture absorption rate was greater in the order of peat+charcoal > peat > charcoal > zeolite. Germination percentage of pelleted seeds with charcoal was the best and similar to that of common non-treated seeds. Germination percentage of pelleted seeds by sowing depth of exposing a quarter of pellet seeds on the surface of vermiculite germination test box and were 92% in charcoal treatment and was the best among all released materials.

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Effects of Seed Size Variation on Germination and Seeding Vigour of Sorghum (Sorghum bicolor L.) (수수의 종자크기가 종자활력과 출아에 미치는 영향)

  • Jung, Ki-Yuol;Yun, Eul-Soo;Park, Chang-Young;Choi, Young-Dae;Hwang, Jae-Bok;Jeon, Seung-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.219-225
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    • 2012
  • Seed size has been considered as an effective criteria for selection of the most vigorous seeds in sorghum [(Sorghum bicolor (L) Moench]. The smaller seeds were inferior to the larger sizes in emergence and grain yield. This study was conducted to determine germination rate, field emergence and vigor of sorghum for selection of high quality seed by different seed size. Sorghum cultivar of two (Hwanggeumchal and Tojong) were separated into five seed size proportion (<2.36, 2.80, 3.15, 3.35 and >3.55 mm diam.) according to seed size. The larger seed was more higher 1,000 seeds weight, seed density, carbohydrates and protein content. Total seed germination performing varied 92% at the largest size (>3.55 mm diam.) frequently inferior to slightly 67% at smaller seed (2.36 to 2.80 mm diam.) in the standard germination test. Seed size did have a significant effect on mean emergence time (MET) and maximum emergence rate index (ERI) and percentage of emergence. It should be noted that the results refer to MET of sorghum seeds ranging from 4.26 to 4.74 days. The relationship of seed size was not only to stand establishment but to grain yield. Yield was most affected by seed size and large seeds were superior to the smaller seed in 25~37% of the cases. Especially, yield was significant under 3.15 mm the beginning.

The Effect of Ginseng Saponin Fraction on the Germination and Early Growth of Rice Seed (Oryza sativa L.) (인삼사포닌이 벼의 발아 및 초기 생장에 미치는 영향)

  • 이용우;주충노
    • Journal of Ginseng Research
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    • v.11 no.1
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    • pp.77-83
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    • 1987
  • The effects of ginseng saponin on the germination and early growth of rice seeds (Oryza sativa L.) were investigated. The early growth (length) of test rice seeds which were rinsed for 60 hrs in $10^{-4}$% saponin solution prior to transplantation to water agar bed was about 20% faster than that of control seeds, It was also found that the amylase activity of the seeds was most active when the seeds were rinsed in 10-4% saponin solution for 48 hrs. In vitro investigation showed that the amylase activities were stimulated about 30% by the treatment of $10^{-5}$% saponin solution compared with control group. From the above results, it seems that the ginseng saponin might activate amylase of rice seed during germination, resulting in rapid growth of rice.

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Differences of Water Absorption Property and Seed Viability according to Morphological Characters in Soybean Genotypes

  • Kim, Seok-Hyeon;Kim, Ji-Na;Chung, Jong-Il;Shim, Sang-In
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.1
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    • pp.59-65
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    • 2006
  • The impermeable seed coat is valuable trait in soybean because impermeable seed retain viability for longer period than permeable seed under adverse conditions such as delayed harvest or prolonged storage. Soybean seeds of various size showing different seed hardness were examined for their water absorption and seed viability under adverse storage conditions. Of one hundred thirty nine genotypes, eight types of seeds having different seed hardness and seed size were used as material. Soybean genotypes showing high hard seed rate, GSI13125 (89%), GSI10715 (54%), and GSI10284 (42%), were slow in water absorption and low in the electroconductivity of seed leachate in distilled water. Germination of GSI10284 and GSI13125 that have higher hard seed rate was less affected by CSVT and artificial aging treatment indicating higher seed storability. The higher storing ability of both collections was confirmed by electroconductivity test for leachate. GSI10122 showed low seedling emergence when the seeds were artificially aged. This genotype was considered as to having a poor storing ability based on difference of electroconductivity before and after artificial aging. Among tests conducted in the experiment, CSVT could be used for determining storage life in legumes. In conclusion, water absorption property of seed was strongly related to the hardness that is directly related to the seed viability and storing ability in soybean seed.

Allelopathic Effects of Artemisia capillaris on the Selected Species (다른 식물에 미치는 사철쑥의 알레로파시 효과)

  • 길봉섭
    • The Korean Journal of Ecology
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    • v.22 no.1
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    • pp.59-63
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    • 1999
  • To verify allelopathic effects, seed germination and seedling growth test, chemical analysis and bioassay of selected species were carried out with naturally occurring chemicals of Artemisia capillaris. Seed germination ratio of Calamagrostis arundinacea. Youngia denticulata and Lactuca indica var. laciniata showed decrease in proportion to increase in aqueous extracts concentration of A. capillaris. while that of Cosmos bipinnatus and Leonurus sibiricus did not. However, dry weight growth of selected species treated with the same extracts as the above experiment was inhibited remarkably compared to the germination test. In the test at different concentrations of essential oil from A. capillaris, seedling growth of A. princeps var. orientalis and Plantago asiatica was suppressed according to the concentration of the essential oil, and root growth of the selected species was more inhibitory than that of shoot growth. Thirty-six chemical compounds were identified from A. capillaris plant by gas chromatography. Seven compounds out of 36 were bioassayed, and terpinen-4-ol was the most toxic among the tested substances.

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