• 한국작물학회지
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• 제46권4호
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• pp.317-320
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• 2001

Sweet com seeds deteriorate faster due to low starch content than field com seeds when stored for a long tenn. This study had been conducted to observe the seed deterioration of four different sweet corns in a long tenn storage conditions in room temperature. Four kinds of sweet com genes (sh2, bt, su, and se) were harvested from 15 days to 50 days after silking with 5-day intervals. These seeds were stored in the room temperature and tested for germination percentages from 3 months to 18 months period with 3-month interval. su seeds germinated better than other types of gene. Hybrid Mecca which is sh2 gene germinated better when stored for 3 months to 18 months. For all genes, mean regression equations in relation to storage periods showed linear responses. For regression equation, the slope of sh2 gene was lower than that of su gene. The highest slope value was observed in bt gene showing faster deterioration rate. The rate at which seed deteriorates seems to be affected by the date at which it was harvested. The seeds that were harvested at the optimum time deteriorated more slowly than those which were not.

• 한국작물학회지
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• 제47권2호
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• pp.123-126
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• 2002

Lipoxygenase might be associated with seed deterioration by catalyzing the incorporation of molecular oxygen into fatty acids and generating free radicals. This study was performed to determine whether seed lipoxygenase activity would alter soybean seed longevity. In this study, germination percentage of lipoxygenase-lacking cultivar Jinpumkong2 (lx1lx1lx2lx2lx3lx3) was lower than that of Taekwangkong (Lx1Lx1Lx2Lx2Lx3Lx3). Segregation ratio for the three lipoxygenase isozymes of the F2-derived from the cross between Taekwangkong and Jinpumkong2 was fitted to 9 (Lx1Lx2Lx3) : 3 (Lx1Lx2lx3) : 3 (lxllx2Lx3) : 1 (lx1lx2lx3), suggesting the tight linkage between the Lx1 and Lx2 loci. Germination percentages varied widely but not differed among lipoxygenase isozyme types of F$_3$ seeds before and after accelerated aging. Seed coat of Jinpumkong2 was damaged severely following accelerated aging, whereas that of Taekwangkong was not. Thus, seed of lipoxygenase-lacking soybean cultivar, Jinpumkong2 showed greater deterioration compared with that of the normal Taekwangkong. However, the presence or absence of lipoxygenase activity had no effect on soybean germination.

• 한국작물학회지
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• 제32권3호
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• pp.268-276
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• 1987

대두종자의 퇴화과정에서 일어나는 생리ㆍ화학적인 변화를 탐색하여 종자세의 차이를 비교하고 종자세 예측에 적합한 방법을 구명하기 위하여 대두 5품종의 종자를 인위노화처리시켰다. 대두종자세의 지표로서 발아율$\times$배축의 길이로 나타낸 값을 매개변수로 하여 각종 종자세 검정성적과 비교검토하였다. 종자세검정의 가장 적합한 방법으로는 발아시험에 의한 방법임을 알 수 있었다. ATP와 GADA 검정법을 제외하고는 모든 검정법이 종자세 지표와 고도의 유의성이 있었다. 대두종자세 검정을 위하여는 배축의 길이. 전기전도도에 의한 검정 및 저온발아 검정법이 적합한 방법임을 알 수 있었다.

• Journal of Ecology and Environment
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• 제38권4호
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• pp.437-442
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• 2015

Studies to estimate seed longevity and dormancy of creeping bentgrass (Agrostis stolonifera L.) were conducted from 2000 to 2005 at Corvallis and Hermiston, Oregon. Seeds from three transgenic glyphosate resistant creeping bentgrass lines, 48-10, 48-13, and ASR368, and one non-transgenic glyphosate susceptible line, SR1020, were used. Creeping bentgrass seeds were buried at 3, 18 and 31 cm in 2000 and removed 6, 12, 18, 24, and 51 months later. Soil type and climatic conditions were different at the two locations. At Corvallis, the soil was a Malabon silty clay loam, and the winters wet and mild. The soil at Hermiston was an Adkins fine sandy loam, and winters drier and colder. Seeds of all creeping bentgrass lines deteriorated faster at Corvallis than at Hermiston. The estimated half-lives of creeping bentgrass lines buried at Corvallis were 8.4 to 20.2 months, while those buried at Hermiston were 8.4 to 37.7 months. At both sites, seeds of the glyphosate resistant lines, 48-10 and 48-13, deteriorated faster than the susceptible line, SR1020. However, seed deterioration in the resistant line, ASR368, was slower than all other creeping bentgrass lines. Based on the germination test, exhumed intact seeds at Corvallis were more dormant than those at Hermiston. If buried, it could be expected that viable creeping bentgrass seeds will persist more than 4 years after the seeds are introduced to a site, but environmental conditions can influence both seed longevity and dormancy.

• 한국산림과학회지
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• 제111권3호
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• pp.418-427
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• 2022

연구는 장기 저장된 소나무 종자의 품질을 평가하고 발아 특성과 생리적 특성 간의 상관 관계를 검증하였으며, 이를 위해 저장 기간과 온도에 따른 종자 발아와 생리적 특성을 조사하였다. 그 결과, 소나무 종자는 4℃에 12년 간 저장되었을 때, 발아율 99%, T50 6.5일로 종자 활력 및 종자세 저하가 거의 일어나지 않았으며, -18℃에 저장할 경우, 저장기간이 더 길어졌다. 또한, 장기 저장에 따른 퇴화 과정 중 활력보다 종자세의 감소가 먼저 일어나는 것으로 나타났다. 종자 침출수의 전기전도도는 4℃에 19년 간 저장되어 활력을 완전히 소실한 종자에서 유의하게 높았으며, 발아율, T50, 평균발아 일수, 발아속도와의 상관관계가 있었다. 침출수에 누출된 무기질 중에서는 K의 농도가 가장 높았으며, 그 다음으로는 Na, Ca, Cu, Mg, Fe 순으로 평균 농도가 높았고, Mn과 Zn은 검출되지 않았다. K, Ca, Cu, Mg, Fe 농도는 처리별로 통계적인 차이가 있었으며, K, Ca, Cu, Na, Mg의 경우, 활력을 완전히 소실한 종자와 그렇지 않은 종자에서 차이를 보였고, Cu의 경우, 2003년에 채집한 종자와 나머지 종자와의 차이가 컸다. 발아율은 Ca, Cu, K, Mg, Na 농도와 부의 상관을 보였으며, 발아균일도는 Cu 농도와 부의 상관을 보였고, 다른 발아 특성은 무기질 농도와 상관이 없었다. 따라서 종자 침출수의 무기질 농도는 개체간의 품질 차이가 적은 경우, 경향이 뚜렷하지 않았으나, 전기전도도는 장기 저장 종자의 활력 뿐 아니라 종자세의 차이를 나타내는 지표가 될 수 있을 것으로 보인다.

• 한국작물학회지
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• 제32권4호
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• pp.417-424
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• 1987

보리종자의 노화과정에서 일어나는 생리화학적인 활성을 비교 검토하여 종자세를 예측하기에 적합한 방법을 구명하기 위하여 새쌀보리, 부호보리 및 두산2002의 3품종을 ‘Wire-mesh tray’ 방법으로 41$^{\circ}C$, 상대습도 100%로 조절하여 1일에서 8일간 인위노화시켰다. 종자세의 지표로서 발아율$\times$유아의 길이로 표시한 값과 각종 매개면수와의 관계를 비교하였다. 발아시험에 의한 방법이 보리종자의 퇴화진전을 확실히 반영해 주고 있으며, ATP 검정과 GADA 검정방법을 제외한 저온발아시험, 전기전도검정 및 테트라조리움 검정방법은 보리 종자세평가에 적합한 방법임을 알 수 있었다. 특히 저온발아시험에 의한 유아장과 데트라조리움 검정법이 가장 효과적인 방법임을 알 수 있었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.31-31
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• 2017

Understanding the mechanism(s) to overcome or prevent seed ageing deterioration during storage is of fundamental interest to seed physiologists. Vitamin E (tocols) is known as a key metabolite to efficiently scavenge lipid peroxy radicals which cause membrane breakdown resulting in seed ageing. However, in rice research this hypothesis has been tested for very few lines only without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing aus, indica, temperate japonica and tropical japonica subpopulations, were used for tocols analysis (quantification of ${\alpha}$-, ${\beta}$-, ${\gamma}$-, ${\delta}$-tocopherol/tocotrienol by ultra performance liquid chromatography) and storage experiments at $45^{\circ}C$ and 10.9% seed moisture content (sample taken for germination testing every 3 days up to 60 days). To examine interactions between DNA sequences and phenotype, the 700k high-density single-nucleotide polymorphism marker data-set was utilized. Both seed longevity (time for viability to fall to 50%; $p_{50}$) and tocols content varied across subpopulations due to heterogeneity in the genetic architecture. Among eight types of tocol homologues, ${\alpha}$-tocopherol and ${\gamma}$-tocotrienol were significantly correlated with $p_{50}$ (negatively and positively, respectively). While temperate japonica varieties were most abundant in ${\alpha}$-tocopherol, indica varieties recorded 1.3 to 1.7-fold higher ${\gamma}$-tocotrienol than those of other subpopulations. It was highlighted that specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.539-546
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• 1996

The physical condition of rapeseed delivered to fat industry plants plays a significant role in the formation of the qualitative features of the raw material for oil production and, consequently , of the oil itself. Rapeseed is stored in silos , frequently for months, before it is subjected to processing. During the long storage, the conditions of the seed cover is very important, as the seed cover provides natural protection of the seed against the effect of the environment. Seeds with damaged seed cover are more easily affected by mildew, and the rate of chemical processes. Deteriorating the quality of oil contained in the cotyledons is faster in such seeds. Cracked seed covers facilitates also the growth and development of microorgaism. So as rapeseed damage occurring inthe course of harvest and the post-harvest processing have a negative effect of the quality and quantity of oil the sees contain. The study presented here was aimed at examining the typical process of purcha ing and handling of rapeseed in fat industry plants, in the aspect of the occurrence of mechanical damage to the seeds. Special attention was paid to the condition of rapeseed immediately after combine harvesting : next , the successive stages of technological handling of the seed were examined. observing the operation parameters of the particular machinery and equipment in order to identify those operations which caused deterioration in the quality of the material (sees). Seed samples were taken successively from the following the hopper, prior to cleaning , after cleaning , prior to drying , after drying, from dry rape silo. The total level of damage increased through the handling. The content of unusable contaminants had the tendency to decrease in the successive operations. though the actual values still exceeded levels permitted by standards. The study allow to indicate the operation fo postharvest technological process, which cause the most seed damage as well as gave quantita ive description of the losses occurred.

• 한국작물학회지
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• 제65권3호
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• pp.248-254
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• 2020

This study was conducted to investigate the impact of harvest time on yield and seed quality in purple seed coat peanut. Compared to the average Virginia peanut variety, 'Heuksaeng', purple peanut variety has been reported to experience yield loss and reduced seed quality under delayed harvest. We examined the yield components and seed quality at different harvest times from 70 to 110 days after flowering (DAF), with harvesting occurring at intervals of 10 days. The number of mature pods per plant reached a maximum of 51.0 at 80 DAF, and average pod and seed yield peaked at 80 DAF with values of 5,229 and 3,532 kg per ha, respectively. Seed quality drastically decreased after 100 DAF, and the concentrations of two major anthocyanin compounds were significantly lower under delayed harvest. The antioxidative activities of DPPH and ABTS decreased to only 69 and 83% of their maximum values at 110 DAF. On the basis of these results, we recommend that 'Heuksaeng' is harvested approximately 10 to 15 days earlier that average Virginia-type cultivars to obtain the highest yield while minimizing the deterioration of seed quality.

• 한국작물학회지
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• 제37권2호
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• pp.171-177
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• 1992

실험실에서 실시한 검정으로 Lotus와 Red clover 종자의 포장출현율 예측에 효과적인 검정방법을 구명하기 위하여 상이한 조건에서 재배된 종자를 퇴화조절검정법, 인위노화검정법과 전기전도도 검정방법으로 검정하였다. 이들을 포장출현율과의 관련성을 구명 하였던 바 1. Lotus와 Red clover의 포장출현율을 예측할 때 퇴화조절검정에서는 (equation omitted)=12.786＋0.65X($R^2$=0.687), 인위노화검정에서는 (equation omitted)=25.989＋0.39X($R^2$=0.260), 전기전도도검정에서는 (equation omitted)=141.932-0.86X($R^2$=0.238)으로써 예측할 수 있었으며 2. 이들 세가지 검정에서의 결과와 포장출현율과는 다음과 같은 다중회귀방정식으로 나타낼수 있었다. (equation omitted)=-198.16＋2.3493X$_1$-1.5564X$_2$＋1.981X$_3$($R^2$=0.916) 여기서, X$_1$, 퇴화조절검정법 ; X$_2$, 인위노화검정법 ; X$_3$, 전기전도도검정임. 3. 이러한 결과는 단일검정법으로 실내에서 포장출현율을 예측하려면 인위노화검정법이나 전기전도검정법 보다도 퇴화조절검정법이 비교적 효율이 높았으며, 이들 세가지 방법을 병용할 때에는 단일검정법보다도 효율을 증가시킬수 있었다.