• Title/Summary/Keyword: seed deterioration

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Seed Deterioration Response of Different Genes of Sweet Corn during Long-tenn Storage

  • Lee, Myoung-Hoon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.4
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    • pp.317-320
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    • 2001
  • Sweet com seeds deteriorate faster due to low starch content than field com seeds when stored for a long tenn. This study had been conducted to observe the seed deterioration of four different sweet corns in a long tenn storage conditions in room temperature. Four kinds of sweet com genes (sh2, bt, su, and se) were harvested from 15 days to 50 days after silking with 5-day intervals. These seeds were stored in the room temperature and tested for germination percentages from 3 months to 18 months period with 3-month interval. su seeds germinated better than other types of gene. Hybrid Mecca which is sh2 gene germinated better when stored for 3 months to 18 months. For all genes, mean regression equations in relation to storage periods showed linear responses. For regression equation, the slope of sh2 gene was lower than that of su gene. The highest slope value was observed in bt gene showing faster deterioration rate. The rate at which seed deteriorates seems to be affected by the date at which it was harvested. The seeds that were harvested at the optimum time deteriorated more slowly than those which were not.

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Relationship of Seed Germination and Lipoxygenase Activity in Soybean

  • Lee, Suk-Ha;Son, Beom-Young;Lee, Yeong-Ho;Lee, Hong-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.2
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    • pp.123-126
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    • 2002
  • Lipoxygenase might be associated with seed deterioration by catalyzing the incorporation of molecular oxygen into fatty acids and generating free radicals. This study was performed to determine whether seed lipoxygenase activity would alter soybean seed longevity. In this study, germination percentage of lipoxygenase-lacking cultivar Jinpumkong2 (lx1lx1lx2lx2lx3lx3) was lower than that of Taekwangkong (Lx1Lx1Lx2Lx2Lx3Lx3). Segregation ratio for the three lipoxygenase isozymes of the F2-derived from the cross between Taekwangkong and Jinpumkong2 was fitted to 9 (Lx1Lx2Lx3) : 3 (Lx1Lx2lx3) : 3 (lxllx2Lx3) : 1 (lx1lx2lx3), suggesting the tight linkage between the Lx1 and Lx2 loci. Germination percentages varied widely but not differed among lipoxygenase isozyme types of F$_3$ seeds before and after accelerated aging. Seed coat of Jinpumkong2 was damaged severely following accelerated aging, whereas that of Taekwangkong was not. Thus, seed of lipoxygenase-lacking soybean cultivar, Jinpumkong2 showed greater deterioration compared with that of the normal Taekwangkong. However, the presence or absence of lipoxygenase activity had no effect on soybean germination.

The Use of Multiple Tests in Predicting the Vigor of Soybean Seeds (대두 종자 발아세 검정방법 비교)

  • Kim, Seok-Hyeon;Copeland, Lawrence O.;Baalbaki, Riad
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.3
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    • pp.268-276
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    • 1987
  • Five soybean varieties were used to measure seed vigor according to the artificially different deterioration. A vigor index derived from the product of percent germination x hypocotyllength was used as the parameter in comparison of other tests for seed vigor. There were the varietal differences in initial vigor. The warm germination test was the best measurement for seed vigor at the advanced stages of seed deterioration. All other vigor measurements, except ATP and GADA measurements, showed highly significant correlations with the vigor index. Hypocotyl length, conductivity index and cold germination measurements for seed vigor were appropriate for predicting seed quality.

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Seed longevity of glyphosate resistant transgenic creeping bentgrass (Agrostis stolonifera L.) lines

  • Hancock, Daniel;Park, Kee Woong;Mallory-Smith, Carol A.
    • Journal of Ecology and Environment
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    • v.38 no.4
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    • pp.437-442
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    • 2015
  • Studies to estimate seed longevity and dormancy of creeping bentgrass (Agrostis stolonifera L.) were conducted from 2000 to 2005 at Corvallis and Hermiston, Oregon. Seeds from three transgenic glyphosate resistant creeping bentgrass lines, 48-10, 48-13, and ASR368, and one non-transgenic glyphosate susceptible line, SR1020, were used. Creeping bentgrass seeds were buried at 3, 18 and 31 cm in 2000 and removed 6, 12, 18, 24, and 51 months later. Soil type and climatic conditions were different at the two locations. At Corvallis, the soil was a Malabon silty clay loam, and the winters wet and mild. The soil at Hermiston was an Adkins fine sandy loam, and winters drier and colder. Seeds of all creeping bentgrass lines deteriorated faster at Corvallis than at Hermiston. The estimated half-lives of creeping bentgrass lines buried at Corvallis were 8.4 to 20.2 months, while those buried at Hermiston were 8.4 to 37.7 months. At both sites, seeds of the glyphosate resistant lines, 48-10 and 48-13, deteriorated faster than the susceptible line, SR1020. However, seed deterioration in the resistant line, ASR368, was slower than all other creeping bentgrass lines. Based on the germination test, exhumed intact seeds at Corvallis were more dormant than those at Hermiston. If buried, it could be expected that viable creeping bentgrass seeds will persist more than 4 years after the seeds are introduced to a site, but environmental conditions can influence both seed longevity and dormancy.

Quality and Physiological Characteristics of Pinus densiflora Seeds Under Different Storage Periods and Temperatures (장기 저장과 저장 온도에 따른 소나무 종자 품질과 생리적 특성)

  • Gu, Da-Eun;Ku, Ja-Jung;Han, Sim-Hee
    • Journal of Korean Society of Forest Science
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    • v.111 no.3
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    • pp.418-427
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    • 2022
  • Our aim was to evaluate the quality of pine seeds after long-term storage and to analyze the correlation between germination and physiological characteristics. Therefore, we investigated the germination and physiological characteristics of seeds stored for different periods and storage temperatures. Pine seeds lost little viability and vigor after 12 years of storage at 4℃; it is even possible to prolong the storage period at -18℃. The deterioration process operating through long-term storage was characterized by a loss of seed vigor before a loss in seed viability. Electrical conductivity of the seed leachate was significantly higher in seeds that completely lost their viability, and was also found to be significantly correlated with the germination percentage, T50, mean germination time, and germination speed. Among the minerals leaked to the leachate, K had the highest concentration, followed by Na, Ca, Cu, Mg, and Fe, while Mn and Zn were not detected. Concentrations of K, Ca, Cu, Mg, and Fe in the leachate differed significantly by treatment, and in the cases of K, Ca, Cu, Na, and Mg, we observed large differences in the leachate between the seeds that were collected in 2003 and those collected in other years.. Germination percentage was significantly negatively correlated to the concentrations of Ca, Cu, K, Mg, and Na. The coefficient of uniformity of germination was significantly negatively correlated to Cu concentration. The other germination characteristics were not significantly correlated with the mineral concentration. Therefore, the mineral concentrations of pine seed leachate were not reliable indicators of seed deterioration when the differences in quality between seed lots were small. However, electrical conductivity could be an indicator of seed viability and vigor of pine seeds under long-term storage.

Vigor Determination in Barley Seeds by the Multiple Criteria (보리 종자세 검정방법 비교)

  • Seok Hyeon, Kim;Zhin Ryong, Choe;Jin Ho, Kang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.4
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    • pp.417-424
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    • 1987
  • The seeds of three barley varieties of which initial seed vigor were different were used to measure seed vigor of accelerated aging techniques. A vigor index derived from the product of percent germination and plumule length was used to estimate seed vigor. The index was compared with the results of other tests. The results of warm germination test well suited to the measurements of seed vigor at the advanced stages of seed deterioration. Other vigor measurements except ATP and GADA values, showed close relationships with the vigor index. The measurements of plumule length in cold test and tetrazolium test were found to be appropriate for predicting seed quality.

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Contrasting rice sub-populations to tocols ratio associated with seed longevity

  • Lee, Jae-Sung;Kwak, Jieun;Yoon, Mi-Ra;Lee, Jeom-Sig;Hay, Fiona R.
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.31-31
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    • 2017
  • Understanding the mechanism(s) to overcome or prevent seed ageing deterioration during storage is of fundamental interest to seed physiologists. Vitamin E (tocols) is known as a key metabolite to efficiently scavenge lipid peroxy radicals which cause membrane breakdown resulting in seed ageing. However, in rice research this hypothesis has been tested for very few lines only without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing aus, indica, temperate japonica and tropical japonica subpopulations, were used for tocols analysis (quantification of ${\alpha}$-, ${\beta}$-, ${\gamma}$-, ${\delta}$-tocopherol/tocotrienol by ultra performance liquid chromatography) and storage experiments at $45^{\circ}C$ and 10.9% seed moisture content (sample taken for germination testing every 3 days up to 60 days). To examine interactions between DNA sequences and phenotype, the 700k high-density single-nucleotide polymorphism marker data-set was utilized. Both seed longevity (time for viability to fall to 50%; $p_{50}$) and tocols content varied across subpopulations due to heterogeneity in the genetic architecture. Among eight types of tocol homologues, ${\alpha}$-tocopherol and ${\gamma}$-tocotrienol were significantly correlated with $p_{50}$ (negatively and positively, respectively). While temperate japonica varieties were most abundant in ${\alpha}$-tocopherol, indica varieties recorded 1.3 to 1.7-fold higher ${\gamma}$-tocotrienol than those of other subpopulations. It was highlighted that specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.

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Monitoring of Rapeseed Damaged During Postharvest Handling

  • Stepniewski, A.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.539-546
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    • 1996
  • The physical condition of rapeseed delivered to fat industry plants plays a significant role in the formation of the qualitative features of the raw material for oil production and, consequently , of the oil itself. Rapeseed is stored in silos , frequently for months, before it is subjected to processing. During the long storage, the conditions of the seed cover is very important, as the seed cover provides natural protection of the seed against the effect of the environment. Seeds with damaged seed cover are more easily affected by mildew, and the rate of chemical processes. Deteriorating the quality of oil contained in the cotyledons is faster in such seeds. Cracked seed covers facilitates also the growth and development of microorgaism. So as rapeseed damage occurring inthe course of harvest and the post-harvest processing have a negative effect of the quality and quantity of oil the sees contain. The study presented here was aimed at examining the typical process of purcha ing and handling of rapeseed in fat industry plants, in the aspect of the occurrence of mechanical damage to the seeds. Special attention was paid to the condition of rapeseed immediately after combine harvesting : next , the successive stages of technological handling of the seed were examined. observing the operation parameters of the particular machinery and equipment in order to identify those operations which caused deterioration in the quality of the material (sees). Seed samples were taken successively from the following the hopper, prior to cleaning , after cleaning , prior to drying , after drying, from dry rape silo. The total level of damage increased through the handling. The content of unusable contaminants had the tendency to decrease in the successive operations. though the actual values still exceeded levels permitted by standards. The study allow to indicate the operation fo postharvest technological process, which cause the most seed damage as well as gave quantita ive description of the losses occurred.

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Effect of Harvest Time on Yield Components and Quality in 'Heuksaeng', the Purple-Skin Peanut Variety

  • Oh, Eunyoung;Pae, Suk-Bok;Park, Jae Eun;Kim, Jung-In;Kim, Sungup;Lee, Myoung-Hee;Ha, Tae Joung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.3
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    • pp.248-254
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    • 2020
  • This study was conducted to investigate the impact of harvest time on yield and seed quality in purple seed coat peanut. Compared to the average Virginia peanut variety, 'Heuksaeng', purple peanut variety has been reported to experience yield loss and reduced seed quality under delayed harvest. We examined the yield components and seed quality at different harvest times from 70 to 110 days after flowering (DAF), with harvesting occurring at intervals of 10 days. The number of mature pods per plant reached a maximum of 51.0 at 80 DAF, and average pod and seed yield peaked at 80 DAF with values of 5,229 and 3,532 kg per ha, respectively. Seed quality drastically decreased after 100 DAF, and the concentrations of two major anthocyanin compounds were significantly lower under delayed harvest. The antioxidative activities of DPPH and ABTS decreased to only 69 and 83% of their maximum values at 110 DAF. On the basis of these results, we recommend that 'Heuksaeng' is harvested approximately 10 to 15 days earlier that average Virginia-type cultivars to obtain the highest yield while minimizing the deterioration of seed quality.

Comparision of Combined Stress Tests for Predicting Field Emergence of Lotus corniculatus and Trifolium pratense (Birdsfoot Trefoil과 Red Clover의 포장출현율 예측을 위한 몇가지 Stress 검정법 비교)

  • Kim, Seok-Hyeon;Choe, Zhin-Ryong;Chung, Min-Hong;Han, Kyeong-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.2
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    • pp.171-177
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    • 1992
  • The purpose of this study was to compare the test methods for predicting field emergence of Lotus corniculatus and Trifolium pratense. Four seed lots of two herbage legumes were controlled deteriorated, accelerated aged before germination test and conductivity of each seed lot was determined. Those germination percent and conductivity of seed lots were compared with actual field emergence rate. Field emergence rate could be estimated by the controlled deterioration test ($R^2$=0.687), by the accelerated aging test ($R^2$=0.260), and by the conductivity test ($R^2$=0.238). A multiple regression equation for predicting field emergence rate(equation omitted) was estimated as (equation omitted)=-198.16 + 2.3493X$_1$-1.5564X$_2$ + 1.9812X$_3$where X$_1$, percent germination of controlled deterioration test ; X$_2$, percent germination of accelerated aging test; and X$_3$, conductivity of solute leakage in the conductivity test. Conclusively, unless multiple tests are not available, the controlled deterioration test was comparatively high efficient for predicting field emergence rate, however, the combined measurements of those three tests can enhance the efficiency.

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