• Title/Summary/Keyword: secondary production

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Plant Regeneration of Hybrid Poplars Through Nodule Culture System (Nodule 배양방법(培養方法)을 이용(利用)한 잡종(雜種)포플러의 식물체(植物體) 재분화(再分化))

  • Chung, Kyung Ho;Chun, Young Woo
    • Journal of Korean Society of Forest Science
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    • v.80 no.1
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    • pp.1-8
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    • 1991
  • Developmental micropropagation method and somatic embryogenesis for hybrid poplars, Populns ehrarnericana Eco28, P. nigra ${\times}$ P. moximowiczii 62-9, were established using nodule culture system. Calli of Eco28 and 62-9 clone were initiated from leaf explant on the medium with 0.5mg/l and 2.0mg/l 2, 4-D, respectively. Cell suspension culture was established from callus derived from leaf explant culture. When suspended on MS medium with optimal combination of BA and NAA fine nodules were obtained after 2 weeks of culture. For shoot regeneration, nodules were transferred into liquid and agar solidified medium. Numerous shoots were regenerated from nodules of 62-9 on liquid media. Organogenesis was effectively achieved on agar solidified regeneration media containing different concentrations of BA and adenine sulfate. Average numbers of 27 and 24 shoots per nodule were induced from 62-1 and Eco28 clones after 8 weeks of culture, respectively. In addition, somatic embryogenesis also occurred in the same regeneration medium. This procedure can be applied to vegetative propagation, utilization of somaclonal variation, production of secondary metabolite and materials of biotechnology research.

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Analysis of Nitrosamines Concentration in Condom by using LC-MS/MS (LC-MS/MS를 이용한 콘돔에 함유된 니트로사민류 농도 분석)

  • Park, Na-Youn;Kim, Sungmin;Jung, Woong;Kho, Younglim
    • Journal of the Korean Chemical Society
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    • v.62 no.3
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    • pp.181-186
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    • 2018
  • Nitrosamines are the nitrosocompounds which are produced by nitrosation reactions of the secondary amine and nitrite, and has been found to be produced through the vulcanization process during the production of rubber products Recently, nitrosamines have been detected in rubber products and become a major topic. Condoms are disposable medical devices, so safety is important because they come into direct contact with the skin and mucous membranes. In this study, we developed an analytical method for nitrosamines in condoms by applying ISO 29941 method. The samples were eluted by distilled water, and target compounds were extracted by liquid-liquid extraction with dichloromethane. And then after concentrated, and quantitatively analyzed by LC-MS/MS. The accuracies of the analytical method were ranged from 85.8 to 108.7%, precisions were lower than 11.5%, and the detection limits were from 0.11 (NDPA and NDBA) to 0.48 (NPYR) ng/mL. Among the 31 condom samples, NDBA was detected from 2 cases by extraction of distilled water, and NDMA were detected from 1 case, NDEA from 4 cases and NDBA from 26 cases by extraction of artificial saliva (pH 4.5). The total amount of nitrosamines in all samples were less than $500{\mu}g/kg$.

Characteristics of Vegetation Structure on the Ridge of the Naknam-Jeongmaek (낙남정맥 마루금 일대의 식생구조 특성)

  • Oh, Koo-Kyoon;Kang, Hyun-Mi;Park, Seok-Gon
    • Korean Journal of Environment and Ecology
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    • v.28 no.6
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    • pp.725-740
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    • 2014
  • To figure out the vegetation situation in the ridge of the Naknam-Jeongmaek, six intensive survey sites were selected considering environmental factors and human interferences, 132 quadrats (area $100m^2$) were installed to survey appearing species of trees and major environmental factors, and the characteristics of vegetation structures were analyzed. The surveyed plots were divided into six groups according to the analysis of classification by TWINSPAN; Quercus mongolica-Q. variabilis community, Pinus densiflora-Q. dentata community, Chamaecyparis obtusa community, Q. mongolica-P. densiflora community, P. densiflora-P. thunbergii community, P. koraiensis community, Rhododendron spp.-Lespedeza cyrtobotrya community. P. densiflora-Q. dentata community, Q. mongolica-P. densiflora community, and P. densiflora-P. thunbergii community are expected to be succeeded by deciduous oaks because the power of deciduous oaks is strong in their lower layer. C. obtusa community, P. densiflora community, and Rhododendron spp.-L.cyrtobotrya community are artificial forests that were artificially formed and are expected to be maintained in the current state for some time because the dominance value of planted species of trees is high. Most vegetations in Naknam-Jeongmaek were secondary forests or artificial forests formed for forest tree production and forestation for erosion control. In particular the top regions and hilly sections of the mountain were mostly dominated by deciduous oaks such as Q. mongolica, Q. variabilis showed some P. densiflora community competing with deciduous oaks. On the other hand, low sections and regions adjacent to the city showed severe artificial interference since exotic species such as P. thunbergiil, C. obtusa, P. koraiensis, and Rhododendron spp. were planted.

Cloning and Functional Analysis of Gene Coding for S-Adenosyl-L-Methionine Synthetase from Streptomyces natalensis (Streptomyces natalensis로부터 S-adenosyl-L-methionine synthetase 유전자의 클로닝 및 기능분석)

  • Yoo, Dong-Min;Hwang, Yong-Il;Choi, Sun-Uk
    • Journal of Life Science
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    • v.21 no.1
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    • pp.96-101
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    • 2011
  • S-Adenosyl-L-methionine synthtase (SAM-s) catalyzes the biosynthesis of SAM from ATP and L-methionine. SAM plays important roles in the primary and secondary metabolism of cells. A metK encoding a SAM-s was searched from Streptomyces natalensis producing natamycin, a predominantly a strong antifungal agent, inhibiting the growth of both yeasts and molds and preventing the formation of aflatoxin in filamentous fungi. To obtain the metK of S. natalensis, PCR using primers designed from the two highly conserved regions for metK genes of Streptomyces strains was carried out, and an intact 1.2-kb metK gene of S. natalensis was cloned by genomic Southern hybridization with PCR product as a probe. To identify the function of the cloned metK gene, it was inserted into pSET152ET for its high expression in the Streptomyces strain, and then introduced into S. lividans TK24 as a host by transconjugation using E. coli ET12567(pUZ8002). The high expression of metK in S. lividans TK24 induced actinorhodin production on R5 solid medium, and its amount in R4 liquid medium was 10-fold higher than that by exconjugant including only pSET152ET.

A geographical study of the Korean Tobacoo forming region (韓國 煙草載培地域의 地理學的 硏究)

  • ;Kim, Kwang Ja
    • Journal of the Korean Geographical Society
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    • v.21
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    • pp.16-37
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    • 1980
  • The purpose of this study is to clarify how the Korean tobacco farming region has been geographically formed by natural and cultural environments, and to examine and cultural environments, and to examine whether it can be recognized as a major part of agricultultural regions. The questions asked in this study are associated with the spatial diffusion of tobacco cultivation, the patterns and processes of the farming region from 1900 to 1960, and the regional characteristics of the concentrated farming area since 1960. The study is inductively approached and most data used were collected from old records and field works. The delineation of the tobacco farming region was derived by applying the concept of the uniform region. Tobacco was introduced through Japan during the years of Kwang Hae Kun (1616-1622). According to the old records, three places, Waekwan, Dongrae, and Ulsan, were the first tobacco raising areas. In the 1700's the well-known tobacco farming regions were scattered all around the nation in places like Jinan, Samdeung, Seongcheon, Gangdong, Yeongweol and Yeongyang. This distributon pattern suggests that tobacco farming in Korea developed spatially along main traffic routes before the 1700's. Untill the 1920's the pattern of tobacco regions was relatively static. Since the 1920's, it has shown a pattern of concentration in the Choongbuk province, where the new highly productive yellow tobacco has been introduced. It was not until the 1960's that yellow tobacco instead of the native variety came to be cultivated all over the country. In the 1960's, the tobacco farming region tended to be concentrated and localized in north western Choongnam, northern Cheonbuk, Choongbuk, and Kyeongbuk including Cheongsong, Andong, and Yeongyang. Since 1970, tobacco production has declined in some of the former major areas of cultivation in terms of its density, while there have appeared highly concentrated areas in Cheongsong and Andong, centered around Yeongyand. There has also emerged a secondary major concentrated area along the coast including such places as Kochang, Yeongkwang and Mooan. The appearance of the Yeongyang tobacco area as the most important core region can be described as follows; at first this area has the disadvantage of being in competition with other places for selling cash crops besides tobacco, because it is located in a mountain zone and it is far from the major metropolises of Seoul and Pusan. Thus has been formed the farming mentality that agricultural management makes the most profit on farming tobacco because tobacco is stable in price and selling routes. As a result of this longstanding belief, these areas (Yeongyang, Cheongsong and Andong) have developed into tobacco concentrated regions. Finally, the tobacco concentrated regions of Korea have changed through time. The factors affecting this change have been the kind of tobacco grown, the monopoly system, agricultural techniques and the expansion of arable land through the clearing of slopes. In conclusion, the research indicates that the localized and concentrated patterns of tobacco cultivation are geographically typical. Thus, recognition of tobacco farming region is important to understanding the agricultural region of Korea as a whole.

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Physical and Chemical Properties of Chlorine Bypass System-Dust from Cement Manufacturing (시멘트 생산 시 발생하는 Chlorine Bypass System-dust의 물리 및 화학적 특성)

  • Han, Min-Cheol;Lee, Dong-Joo
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.7 no.4
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    • pp.310-315
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    • 2019
  • This study conducted a series of studies to find alternative ways to use Chlorine Bypass System-dust(CBS-dust) in cement production. The results of engineering characteristics of CBS-dust are summarized as follows. First of all, the density of CBS-dust is 2.40, lighter than cement and the pH was 12.50 which was strong alkaline. In terms of particle size, it was 11.70 ㎛ which was finer than cement. With chemical properties, calcium oxide(CaO) was the highest as 35.10%, potassium oxide(K2O) was 32.43%, potassium chloride(KCl) was 19.46%, sulfur oxide(SO3) was 6.81%, and the remaining chemical components are SiO2, Fe2O3, Al2O3, MgO, and the like. Therefore, if CBS-dust is used as early-strength chemical admixtures in the concrete secondary products that use a large amount of mineral admixtures without rebar, it can be an effective method for increasing the strength of concrete as an alkali activator and preventing early-frost damage of Cold Weather Concrete.

Effect of cadmium on immune responses and enzyme activities in BALB/c mouse 2. Humoral immune responses (카드뮴이 BALB/c 마우스의 면역반응 및 효소활성에 미치는 영향 2. 체액성 면역반응)

  • Yoon, Chang-yong;Cho, Jeong-gon;Song, Hee-jong
    • Korean Journal of Veterinary Research
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    • v.36 no.4
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    • pp.839-844
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    • 1996
  • This study was designed to investigated the effects of cadmium(Cd) feeding on the humoral immune responses such as PFC-responses and production of immunoglobulins in BALB/c mice. The results obtained were summarized as follows; 1. Total PFCs of direct IgM antibody response were significantly decreased in all Cd-fed goups, whereas total PFCs of IgG antibody response were slightly increased. 2. In secondary immunization, total HA titers were increased in all Cd groups as compared with control, especially in 100ppmm group and also IgG titers were slightly increased except for 50ppm group. 3. The levels of $IgG_1$ were increased to 5.5% 18.7%, 17.4% and 7.2% in 25, 50, 100 and 200ppm groups as compared with control, respectively. And also the levels of IgE were increased to 5.7%, 7.3%, 8.7% and 0.4% in those of Cd groups, in order. Conclusively, concentrations of $IgG_1$, and IgE were increased in all Cd groups. Based on the results of this study and previous report, it was shown that Cd might affect humoral immune responses by modifying the distribution and function of cells playing in the cellular immune response.

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The Effect of Douchi Hataedock Treatment for Dermatophagoides Farinae-Induced Atopic Dermatitis-like Skin Lesions by Controlling IL-4 Activity (두시 하태독법의 IL-4 활성 조절이 D. farinae 유도 아토피유사피부염 발병 조절에 미치는 효과)

  • Ahn, Sang Hyun;Kim, Jae Kyu;Cheon, Jin Hong;Kim, Ki Bong
    • The Journal of Pediatrics of Korean Medicine
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    • v.31 no.1
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    • pp.43-51
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    • 2017
  • Objectives Hataedock method is a Korean medical therapy which removes fetal toxin by orally administering herbal decoction to neonates. This study was to observe skin damage and anti-inflammatory effect via regulating IL-4 activity in NC/Nga mice which were induced atopic dermatitis (AD)-like skin lesion by Dermatophagoides (D.) farinae after applying Douchi Hataedock method. Methods NC/Nga mice with 3 weeks of gestational age were used. Each 10 mice were allocated to the control group (Ctrl), the AD-induced group (AE), and the group which induced AD after administering Douchi extract (GT). After 4 weeks from administering Douchi extract to the mice, the primary AD was induced by applying D. farinae extract 6 times per week for 3 weeks and then the secondary AD was induced by the same method after 1 week from the primary AD induction. To identify the skin damage and anti-inflammatory effect, we observed LxR, IL-4, Fc ${\varepsilon}$ receptor, substance P, and $NF-{\kappa}B$. Results The GT group showed alleviation of skin injury and decrease in capillary angiogenesis. Stratum corneum damage, epithelial cell hyperplasia, lymphocyte infiltration, and capillary distribution relatively decreased in the GT group. LxR-positive reaction in the GT group were increased by 53% than that of the AE group. IL-4 production, $Fc{\varepsilon}$ receptor activity, and substance P-positive reaction in the GT group were decreased by 82%, 42%, and 82% respectively compare to those of the AE group. $NF-{\kappa}B$-positive reaction in the GT group were decreased by 15% compare to that of the AE group. Conclusions Hataedock method with Douchi extract alleviated AD via reducing inflammatory cytokines secreted at the early stage of AD. Thus, Douchi Hataedock method has a beneficial effect for the prevention and treatment of AD.

Characterization of the Immune Regulation Function of Fibroblastic Reticular Cells Originating from Lymph Node Stroma (림프절 스트로마 유래 fibroblastic reticular cell의 면역조절 기능에 대한 특성 규명)

  • Lee, Jong-Hwan
    • Journal of Life Science
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    • v.26 no.7
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    • pp.789-795
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    • 2016
  • A lymph node (LN) is one of the secondary lymphoid organs. An LN consists of a complicated 3 dimensional frame structure and several stromal cells. Fibroblastic reticular cells (FRC) are distributed in the T zone for interaction with T cells. FRC secrete homing chemokines such as CCL19 and CCL21. Moreover, FRC play a pivotal role in the production of extracellular matrix (ECM) into LN for ECM reorganization against pathogen infections. However, not much is known about the involvement of the immune reaction of FRC. The present report is for the characterization of FRC on immune response. For this, FRC were positioned in several infected situations such as co-culture with macrophage, lipopolysaccharide (LPS), and TNFα stimulation. When a co-culture between FRC and macrophage was performed, a morphological change in FRC was observed, and empty space between FRCs was created by this change. The soluble ICAM-1 protein level was up-regulated by co-culturing with Raw264.7 and the treatment of the ROCK inhibitor Y27632. The activity of matrix metalloproteinase (MMP) was up-regulated by LPS onto FRC. Furthermore, the inflammatory cytokine TNFα regulated the expression of ECM in FRC by a gene chip assay. Collectively, it suggests that FRC are involved in immune reactions.

Rice OsACDR1 (Oryza sativa Accelerated Cell Death and Resistance 1) Is a Potential Positive Regulator of Fungal Disease Resistance

  • Kim, Jung-A;Cho, Kyoungwon;Singh, Raksha;Jung, Young-Ho;Jeong, Seung-Hee;Kim, So-Hee;Lee, Jae-eun;Cho, Yoon-Seong;Agrawal, Ganesh K.;Rakwal, Randeep;Tamogami, Shigeru;Kersten, Birgit;Jeon, Jong-Seong;An, Gynheung;Jwa, Nam-Soo
    • Molecules and Cells
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    • v.28 no.5
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    • pp.431-439
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    • 2009
  • Rice Oryza sativa accelerated cell death and resistance 1 (OsACDR1) encodes a putative Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK). We had previously reported upregulation of the OsACDR1 transcript by a range of environmental stimuli involved in eliciting defense-related pathways. Here we apply biochemical, gain and loss-of-function approaches to characterize OsACDR1 function in rice. The OsACDR1 protein showed autophosphorylation and possessed kinase activity. Rice plants overexpressing OsACDR1 exhibited spontaneous hypersensitive response (HR)-like lesions on leaves, upregulation of defense-related marker genes and accumulation of phenolic compounds and secondary metabolites (phytoalexins). These transgenic plants also acquired enhanced resistance to a fungal pathogen (Magnaporthe grisea) and showed inhibition of appressorial penetration on the leaf surface. In contrast, loss-of-function and RNA silenced OsACDR1 rice mutant plants showed downregulation of defense-related marker genes expressions and susceptibility to M. grisea. Furthermore, transient expression of an OsACDR1:GFP fusion protein in rice protoplast and onion epidermal cells revealed its localization to the nucleus. These results indicate that OsACDR1 plays an important role in the positive regulation of disease resistance in rice.