• 한국식품영양과학회지
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• 제27권5호
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• pp.952-959
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• 1998

To investigate the effect of sea tangle on macrophage activity in normal and diabetic states, 10week old ICR mice were fed control(C) and sea tangle(S) diet containing 5%(w/w) cellulose and 13.6%(w/w) dry sea tangle for four weeks, after which two thirds of mice(CD and SD) were made diabetic by intramuscular injection of streptozotocin(150mg/kg bw). At 4th day after diabetes was apparent by urinary glucose, one half of diabetic mice(CDM and SDM) were treated with metformin(500mg/kg bw) orally. Peritoneal macrophages obtained from 3%-thioglycollate treated mice were cultured in the presence of lipopolysaccaride from Salmonella abortus equi(10$\mu\textrm{g}$/ml) for 24 hrs and tumor necrosis factor-$\alpha$(TNF$\alpha$), interleukin-1$\beta$(1L-1$\beta$)and prostaglandin E2(PGE2) were measured in culture media. Release of IL-1$\beta$and PGE2 from macrophage were increased in normal mice by sea tangle diet and had the same tedency in diabetic mice with or without metformin treatment although not statistically significant. Release of TNF$\alpha$ tended to be reduced by diabetes but were not changed significantly by sea tangle diet. Fatty acid compositions of macrophage and liver phospholipids showed that diabetes reduced arachidonic acid/linoleic acid ratio and sea tangle diet appeared to increase contentsof polyunsaturated fatty acids.

• 한국식품영양과학회지
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• 제23권1호
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• pp.156-163
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• 1994

To investigate the optimum conditions of seaweed Mooks prepared with sea mustard (Indaria pinnatifida) and sea tangel(Laminaria japonica), pH, viscosity , yield and jelly strength were studied on the sol and /or gel (Mook ) made from homogenized seaweeds. Solubilization conditions of homogenized seaweeds were heating at $65^{\circ}C$ for one hour after mixing homogenized seaweed with 1 % $K_2$HPO$_4$ by 1 ; 1 (v/v) . Gelation conditions were 24 hrs by natural permeation of Ca++ into the sol in three times (v/v) of 1 % CaCl$_2$ solution to solubilized seaweed passed through 60 mesh of sieve. There are generally no relationship between viscosity of solubilized seaweed and jelly strength of Mook produced by gelation of the sol in CaCl$_2$ solution . Jelly strength of sea mustard Mook was more than 500g/$\textrm{cm}^2$ , yield was 87.5% of the solubilized and filtrated seaweed . Jelly strength and specific gravity of sea tangle Mook were higher those of sea mustard Mook, while its yield was lower than that of sea mustard Mook.

• 한국식품영양과학회지
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• 제27권5호
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• pp.960-967
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• 1998

The present study was conducted to investigate the effect of sea tangle and hypoglycemic agent on lipid metabolism in normal and dabetic rats. Male Sprague-Dawely rats were fed AIN-76 based experimental diets containing 5%(w/w) cellulose or 15%(w/w) sea tangle for 3 weeks, after which diabetic groups were made diabetic by intramuscular injection of streptozotocin(STZ, 45mg/kg BW). Metformin(350mg/kg BW) as a hypoglycemic agent was given once a day using a feeding tube for 5 days. Body weight grains were reduced significantly by STZ treatment, but not influenced by metformin feeding. Blood glucosel levels in sea tangle groups were reduced, compared with those in cellulose groups. Metformin feeding showed the lowering effect of blood glucose. Plasma levels of triglyceride were increased significantly in diabetic rats, but decreased in metformin group by sea tangel feeding. Total cholestero contents showed a similar tendency with triglyceride, but were reduced in diabetic groups without metformin by sea tangle feeding. Plasma levels of HDL-cholesterol were reduced in diabetic rats, compared with those in normal rats. There was a significant increase in fecal weights in diabetic rats fed sea tangle. Fecal contents of cholesterol were lower in diabetic rats than in normal rats. In normal rats, it tended to increase by sea tangle feeding, but not significantly. Fecal excretions of coprostanol and coprostanone were reduced significantly in diabetic rats, compared with those of normal rats. It tended to increase in diabetic rats by simultaneous feeding of sea tangle and metformin, but not significantly. Diabetes reduced fecal excretion of bile acid, but it was increased by sea tangle and metformin feeding.

• 생명과학회지
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• 제11권5호
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• pp.476-482
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• 2001

다시마의 열수출물이 당뇨병의 치료에 효과가 있는지를 알아보기 위하여 alloxan으로 처리하여 실험적으로 당뇨병을 유발한 후 glucose의 초기 인산화에 관여하는 glu-cokinase와 hexokinase의 활성을 살펴본 결과 다음과 같은 결론을 얻었다. 1. Alloxan 주사에 의해 체중과 공복시의 glucose 및 insulin 분비가 증가되었으나, 다시마 투여에 의해 공복시의 insulin 분비가 유의성있게 감소하였으며 체중과 공복시의 glucose의 유의성은 없었으나 대조군에 비하여 감소하였다. 2. Alloxan 주사에 의해 혈청중 glucosecl가 정상군에 비하여 현저하게 증가되었으나 다시마 투여에 의해 현저히 감소되었다. Insulin 치의 상승과 분비 지연도 다시마 투여군에서는 정상군과 유사한 경향을 보였다. 3. Glucokinase와 hexokinase의 활성은 alloxan 주사에 의해 현저히 감소되었으나, 다시마 투여에 의해 유의성있게 상승되었다. 이상의 결과로 다시마는 alloxan으로 유발된 당뇨병에서 glcuose 인산화 glucokinase와 hexokinase의 활성을 증가시키는 것으로 나타나 당뇨병의 치료에 효과가 있는 것으로 여겨진다.