• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.5
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• pp.952-959
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• 1998

To investigate the effect of sea tangle on macrophage activity in normal and diabetic states, 10week old ICR mice were fed control(C) and sea tangle(S) diet containing 5%(w/w) cellulose and 13.6%(w/w) dry sea tangle for four weeks, after which two thirds of mice(CD and SD) were made diabetic by intramuscular injection of streptozotocin(150mg/kg bw). At 4th day after diabetes was apparent by urinary glucose, one half of diabetic mice(CDM and SDM) were treated with metformin(500mg/kg bw) orally. Peritoneal macrophages obtained from 3%-thioglycollate treated mice were cultured in the presence of lipopolysaccaride from Salmonella abortus equi(10$\mu\textrm{g}$/ml) for 24 hrs and tumor necrosis factor-$\alpha$(TNF$\alpha$), interleukin-1$\beta$(1L-1$\beta$)and prostaglandin E2(PGE2) were measured in culture media. Release of IL-1$\beta$and PGE2 from macrophage were increased in normal mice by sea tangle diet and had the same tedency in diabetic mice with or without metformin treatment although not statistically significant. Release of TNF$\alpha$ tended to be reduced by diabetes but were not changed significantly by sea tangle diet. Fatty acid compositions of macrophage and liver phospholipids showed that diabetes reduced arachidonic acid/linoleic acid ratio and sea tangle diet appeared to increase contentsof polyunsaturated fatty acids.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.1
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• pp.156-163
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• 1994

To investigate the optimum conditions of seaweed Mooks prepared with sea mustard (Indaria pinnatifida) and sea tangel(Laminaria japonica), pH, viscosity , yield and jelly strength were studied on the sol and /or gel (Mook ) made from homogenized seaweeds. Solubilization conditions of homogenized seaweeds were heating at $65^{\circ}C$ for one hour after mixing homogenized seaweed with 1 % $K_2$HPO$_4$ by 1 ; 1 (v/v) . Gelation conditions were 24 hrs by natural permeation of Ca++ into the sol in three times (v/v) of 1 % CaCl$_2$ solution to solubilized seaweed passed through 60 mesh of sieve. There are generally no relationship between viscosity of solubilized seaweed and jelly strength of Mook produced by gelation of the sol in CaCl$_2$ solution . Jelly strength of sea mustard Mook was more than 500g/$\textrm{cm}^2$ , yield was 87.5% of the solubilized and filtrated seaweed . Jelly strength and specific gravity of sea tangle Mook were higher those of sea mustard Mook, while its yield was lower than that of sea mustard Mook.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.5
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• pp.960-967
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• 1998

The present study was conducted to investigate the effect of sea tangle and hypoglycemic agent on lipid metabolism in normal and dabetic rats. Male Sprague-Dawely rats were fed AIN-76 based experimental diets containing 5%(w/w) cellulose or 15%(w/w) sea tangle for 3 weeks, after which diabetic groups were made diabetic by intramuscular injection of streptozotocin(STZ, 45mg/kg BW). Metformin(350mg/kg BW) as a hypoglycemic agent was given once a day using a feeding tube for 5 days. Body weight grains were reduced significantly by STZ treatment, but not influenced by metformin feeding. Blood glucosel levels in sea tangle groups were reduced, compared with those in cellulose groups. Metformin feeding showed the lowering effect of blood glucose. Plasma levels of triglyceride were increased significantly in diabetic rats, but decreased in metformin group by sea tangel feeding. Total cholestero contents showed a similar tendency with triglyceride, but were reduced in diabetic groups without metformin by sea tangle feeding. Plasma levels of HDL-cholesterol were reduced in diabetic rats, compared with those in normal rats. There was a significant increase in fecal weights in diabetic rats fed sea tangle. Fecal contents of cholesterol were lower in diabetic rats than in normal rats. In normal rats, it tended to increase by sea tangle feeding, but not significantly. Fecal excretions of coprostanol and coprostanone were reduced significantly in diabetic rats, compared with those of normal rats. It tended to increase in diabetic rats by simultaneous feeding of sea tangle and metformin, but not significantly. Diabetes reduced fecal excretion of bile acid, but it was increased by sea tangle and metformin feeding.

• Journal of Life Science
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• v.11 no.5
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• pp.476-482
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• 2001

The study was undertaken to determine the effect of the aqeuous extract of sea tangle, Laminaria japonicus, on the activites of glucokinase and hexokinase in the pancreas of diabetic mellitus mice which were induced by alloxan. After one week of alloxan injection, the levels of serum glucose and insulin secretion were dramatically increased, however, the insulin secretion was decreased with administration of sea tangle extract. Alloxan injection allowed the serum glucose level to increase and level was decreased by sea tangle extract administration. Furthermore, it was observed the sea tangle extract was effective in recovering the levels of insulin secretion. Enzyme activities of the glucokinase and hexokinase were decreased by alloxan treatment. In contrast, sea tangle extract administration to the mice allowed to increase proportionally. These results suggested that sea tangle extract is highly effective in treatment of diabetic mellitus induced by alloxan.