• Asian-Australasian Journal of Animal Sciences
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• 제17권4호
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• pp.441-444
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• 2004

The genetic diversities and relationships of 10 Chinese indigenous pig breeds and three exotic pig breeds have been evaluated using 26 microsatellites recommended by the Food and Agriculture Organization & the International Society of Animal Genetics (FAO-ISAG). The allele frequencies, genetic heterozygosity (H) and polymorphism information content (PIC) have been calculated. The results showed that genetic diversity of Chinese indigenous pig breeds is higher than that of the introduced pig breeds. The clustering of 10 breeds is generally consistent with their geographical distribution.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.117.1-117.1
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• 2006

• 한국초등과학교육학회지:초등과학교육
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• 제38권2호
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• pp.203-215
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• 2019

The purpose of this study is to clarify the roles of laboratory work in school. Twenty-one literatures were identified about roles of laboratory work in school by applying criteria such as published in CI level journals or used as a reference over 100 times during 1960-2017. Twenty-one literatures were reviewed according to periods such as 1960-1989, 1990-2003, and 2004-2017, and identified the roles of laboratory work in school that commonly presented in more than two literature. Seven roles of laboratory work in school identified were as follows (a) learning scientific knowledge, (b) enhancing attitude toward science, (c) learning scientific inquiry methods, (d) acquiring skills to use specific laboratory instruments, (e) enhancing scientific attitude, (f) understanding the nature of science(NOS), and (g) providing opportunity to experience natural or scientific phenomena. The results of this study can be used to provide school teachers and students a clear meaning of the roles of laboratory work in school.

• BMB Reports
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• 제42권6호
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• pp.344-349
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• 2009

Angiogenesis is crucial for solid tumor growth. By secreting angiogenic factors, tumor cells induce angiogenesis. However, targeting these angiogenic factors for cancer therapy is not always successful, suggesting that other factors may be involved in tumor angiogenesis. This work shows that 25 protein spots were differentially expressed by two-dimensional gel electrophoretic analysis when HepG2 cells induced endothelial cell differentiation to tube in vitro, and most of them were upregulated. Twenty-one proteins were identified with MALDITOF-MS, and the other four were identified by LTQ-MS/MS. Keratins were identified as one class of these upregulated proteins. Further study indicated that the expression of keratin 17 in cultured endothelial cells is likely microenvironment regulated, because its expression can be induced by HepG2 cells and bFGF as well as serum in culture media. Increased expression of keratins in endothelial cells, such as keratin 17, may contribute to the angiogenesis induced by HepG2 cells.

• Interdisciplinary Bio Central
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• 제2권4호
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• pp.12.1-12.7
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• 2010

The National Bio Resource Project-Rat (NBRP-Rat) comprises the largest bank of laboratory rat (Rattus norvegicus) strains in the world. Its main focus is to develop infrastructure that will facilitate the systematic collection, preservation, and provision of rat strains. To breed effectively more than 180 rat strains in living stock, we establish the genetic control system in which a systematic set of genetic diagnoses and genetic monitoring are included. Genetic monitoring is performed by using 20 polymorphic markers. Monitoring is carried out when a living animal stock is re-established by using cryopreserved embryos or sperm or when a rat strain is first introduced to the NBRP-Rat by a depositor. Additional monitoring is then carried out on each strain every two years. Genetic diagnosis is performed largely by employing the Amp-FTA method. Protocols which detail how to perform a genetic diagnosis of 11 transgenes and 24 mutations have been made. Among the mutations, nine can be detected by simple gel electrophoresis of the PCR products, 11 by restriction enzyme treatment of the PCR products, and four by direct PCR product sequencing. Using this genetic control system, the NBRP-Rat can guarantee the genetic quality of its rat strains.

• 한국과학교육학회지
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• 제26권5호
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• pp.660-673
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• 2006

There have been many attempts to determine the value and the role of school laboratory experiment, but it seems hard to find consensus among these attempts from the perspective of education. This difficulty seems mainly due to disagreement on the concept of education, which has caused an instrumental attitude considering the school laboratory only as a means of developing science or pursuing various functions of school. However, the Endogenous Theory of Education (ETE), which claims education as 'a form of life', has recently paved the way for laboratory experiment to be justified as an opportunity of 'educooperation' allowing students to experience the intrinsic values of education in the medium of science. According to this theory, it is not the detailed practicals but the whole context where the laboratory activity is situated that matters in revealing the inherent educational phenomena. Through this new perspective, I observed two biology laboratory classes in a high school and analyzed the pattern of teacher-student and student-student interactions. Some meaningful educooperation was found in students' chattering, which has been traditionally considered as merely noise in the classroom, rather than in teacher-student interactions. This study discusses the reasons for these findings in detail and culminates in suggesting ways for accentuating the educational aspect of school laboratory activity.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.74-74
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• 2002

No Abstract, See Full Text

• 한국과학교육학회지
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• 제23권3호
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• pp.254-264
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• 2003

실험활동은 과학교육에서 중요한 요소로서, 그 목적과 효과에 대해 많은 논의가 있어왔다. 그러나 이에 관한 연구결과는 회의적이며 그 이유 중 하나는 하나의 실험은 동시에 다양한 목적을 성취할 수 없으며, 교사와 학생들이 실험의 목적에 대해 인식하고 있지 않기 때문이라는 것이다. 그러므로 본 연구에서는 시험의 목적에 대한 중학생들의 인식을 조사하였다. 실험의 목적에 대한 중학생들의 인식을 알아보기 위해 '과학자들의 실험'과 '학교실험'의 두 가지 상황에 대해 서울 소재 중학교 1학년생 147명을 대상으로 실험의 목적에 대해 서술형으로 응답하도록 하였다. 응답을 분석한 결과, 학생들의 56.8%가 과학자들이 새로운 것을 발견하거나 발명하기 위해 시험을 하는 것으로 응답하였으며, 학교실험에 대해서는 82.9%의 학생들이 인지적 영역, 즉 교과서에서 배운 것을 이해하거나 암기하기 위한 것이라고 응답하였다. 성별과 성취도에 따른 응답비율의 차이를 조사한 결과 남학생들의 경우에는 사회적 유용성을, 여학생의 경우에는 개인적 즐거움을 더 많이 응답하였다. 과학자의 시험과 학교실험에 대한 인식의 상화관련을 조사한 결과, 과학자들의 실험이 이론을 증명하는 것이라고 생각하는 학생은 학교 시험의 기능도 유사하게 생각하였다. 마지막으로 학생들의 응답을 정성적으로 분석하였으며, 결론적으로 학생들은 실험에 대해 다양하고 적절한 인식을 가지고 있지 못하며, 학교실험에 대해 회의적인 태도를 보이기도 하였다. 이를 개선하기 위해서 교사는 시험의 다양한 목적에 대해 학생들에게 알려주고, 매 시험마다 그 실험의 목적에 대해 명확히 밝혀야 할 것이다.

• 한국환경교육학회지:환경교육
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• 제20권4호
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• pp.65-83
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• 2007

The purpose of this study was to survey science teachers, perception and attitudes on disposal of school laboratory waste water and materials. To fulfill this aim, a questionnaire was administered to 244 secondary school science teachers. The result of this study revealed that there was no large disparity in the degree of understanding of laboratory waste disposal and the laboratory waste water treatment, and in secondary science teachers' perception and practicing attitude on the environment. While science teachers had deep understanding of laboratory waste disposal and its influence on environmental pollution, they expressed less positive attitude toward the actual disposal of waste materials and waste water, and participation in teacher training. Therefore, it is necessary to provide standards of systematized disposal methods and proper disposal facilities in middle and high school laboratories, as well as to investigate teachers' needs before providing teacher training program.

• Toxicological Research
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• 제21권2호
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• pp.179-186
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• 2005

Dimethylamine (DMA) is a widely used commodity chemical with few toxicity data. Groups of 10 male and female F-344 rats were exposed by inhalation to 0, 5, 10, 20, 40 and 80 ppm of DMA for 6 hrs/day, 5 days/week for 90 days. The changes of body weight, organ weight, hematology, clinical chemistry, and histopathological changes were evaluated after the exposure. As the results, the body weight was significantly decreased at 80 ppm in male and female rats (p<0.05). The absolute lung weight showed no statistically significant changes in any group. In contrast, the relative lung weight significantly increased at 80 ppm in male and female rats (p<0.05). Erythrocytes, mean cell hemoglobin, leukocytes, neutrophil, and platelet numbers were significantly increased in male and female at 40 or 80 ppm of DMA (p<0.05, p<0.01). In addition, the serum values of total protein, urea nitrogen were increased in male and creatine kinase, total protein were increased in female rats at 40 or 80 ppm (p<0.05, p<0.01). Histopathological examinations of the male and female lung samples showed slight hyperplasia and congestion at 80 ppm. Taken together, our study revealed that maximum tolerated dose of DMA would be over 40 ppm.