Characterization of the analysis of forest vegetation, soil environmental conditions and water quality were performed from March 2003 to March 2007. The two basins were characterized by cultivated area (Kaesim reservoir) and mountain area (Jangchan reservoir), and divided into eleven small basins, where dynamics of pollutants, forest vegetation and soil environmental conditions were surveyed. The vegetation can be divided into 10 types by $Z\ddot{u}rich$-Montpellier school's method. Pearson coefficients between vegetation type and water quality were correlated with dissolved oxygen (DO) in the Quercus variabilis community at the 5% level and total phosphorus (TP) in the Larix leptolepis plantation at the 1% level. Especially total phosphorous and total nitrogen increased in small basins where the proportion of cultivated and residential area increased. The analysis of influences of pollutant discharge on water quality showed that pollutant charge was very low in forest land area ($Y_{T-P}$=-0.0017X+0.2215, r=0.16, $Y_{COD}$=- 0.0395X+8.5051 r=0.47). The soil types of western area were comparatively simple, but those of eastern area were complicated with regosols, red-yellow soils, lithosoles, etc. The pH, total solid (TS) and volatile substance (VS) of the forest and agricultural land soils collected in each site were 5.4~6.9, 75.8~80.2%, and 3.80%~5.80%, respectively. According to the analytical result of soil environmental conditions, heavy metal contents fell short to the mean value of natural conditions. Runoff amount (Y) and depth of topsoil (X) were negatively correlated, $Y_{ron}=-1.0088X_{top}+35.378$ (r=0.68). The correlation was much lower in up-stream but much higher in down-stream, because permeation into soil particle was larger on down-stream due to its more or less gentle slope. Pearson coefficients between soil pH and water pH were statistically significant at 1% level.
This study was performed to predict the future climate envelope of Pinus pumila, a subalpine plant and a Climate-sensitive Biological Indicator Species (CBIS) of Korea. P. pumila is distributed at Mt. seorak in South Korea. Suitable habitat were predicted under two alternative RCPscenarios (IPCC AR5). The SDM used for future prediction was a Maxent model, and the total number of environmental variables for Maxent was 8. It was found that the distribution range of P. pumila in the South Korean was $38^{\circ}7^{\prime}8^{{\prime}{\prime}}N{\sim}38^{\circ}7^{\prime}14^{{\prime}{\prime}}N$ and $128^{\circ}28^{\prime}2^{{\prime}{\prime}}E{\sim}128^{\circ}27^{\prime}38^{{\prime}{\prime}}E$ and 1,586m~1,688m in altitude. The variables that contribute the most to define the climate envelope are altitude. Climate envelope simulation accuracy was evaluated using the ROC's AUC. The P. pumila model's 5-cv AUC was found to be 0.99966. which showed that model accuracy was very high. Under both the RCP4.5 and RCP8.5 scenarios, the climate envelope for P. pumila is predicted to decrease in South Korea. According to the results of the maxent model has been applied in the current climate, suitable habitat is $790.78km^2$. The suitable habitats, are distributed in the region of over 1,400m. Further, in comparison with the suitable habitat of applying RCP4.5 and RCP8.5 suitable habitat current, reduction of area RCP8.5 was greater than RCP4.5. Thus, climate change will affect the distribution of P. pumila. Therefore, governmental measures to conserve this species will be necessary. Additionally, for CBIS vulnerability analysis and studies using sampling techniques to monitor areas based on the outcomes of this study, future study designs should incorporate the use of climatic predictions derived from multiple GCMs, especially GCMs that were not the one used in this study. Furthermore, if environmental variables directly relevant to CBIS distribution other than climate variables, such as the Bioclim parameters, are ever identified, more accurate prediction than in this study will be possible.
The purpose of this study is to provide some suggestions for future improvement of scientific inquiry activities in Korean elementary science textbook. The modified framework of Lee(2005) and Millar et al.(1998) was used to compare inquiry activities in the Korean and Singaporean science textbooks. The results of this study are as follows: Korean text books have more activities than Singapore's, but both countries have similar time allotment for science classes. In the area of 'inquiry process skill', Singapore is more balanced in 'Basic inquiry process skills' and 'Integrated inquiry process skills' than Korea. Singapore's integrated inquiry rate is also higher than Korea's. Next the results of comparing leaning objectives to scientific inquiry activities shows that Korean text books tend to focus on 'contents objectives', while Singapore's text books focus on balancing 'contents objectives' and 'process objectives'. Korean science textbooks encourage students to communicate the results of experiments but in most case these communication activities are actually not performed. Lastly Korea and Singapore have low degree of openness in inquiry activities. Remarkably 'Suggest questions' are totally conducted by teachers. This study implies that Korean science textbooks should have lower amounts of inquiry activities to accomodate enough time for communication about results. Next we need to make balance not only 'Basic inquiry process skills' and 'Integrated inquiry process skills' but also 'Content objectives' and 'Process objectives'. Lastly we need to make student to be the leader in science classes through encouraging them to plan procedures for experiments and to discover results by themselves.
Studies have observed various phenomena regarding the effect of the interaction between type, price, and brand image of a product on consumers' purchase intent and product quality perception. Yet, few have studied the effect of the interaction between product type and manufacturing method on these factors. However, the advent of three-dimensional (3D) printers added a new manufacturing method, 3D printing, to the traditional methods of handicraft and automated machine-based production, and research is necessary since this new framework might affect consumers' purchase intent and product quality perception. Therefore, this study aimed to verify the effects of the interaction between product type and manufacturing method on purchase intent and product quality perception. To achieve this, in our experiment 1, we selected product types with different characteristics (drone vs. violin vs. cup), and measured whether consumers preferred different manufacturing methods for each product type. The results showed that consumers preferred the 3D printing method for technologically advanced products such as drones, the handmade method for violins, and the automated machine-based manufacturing method, which allows mass production, for cups. Experiment 2 attempted to verify the effects of the differences in manufacturing method preferences for each product type on consumers' purchase intent and product quality perception. Our findings are as follows: for drones, the purchase intent was highest when 3D printing was used; for violins, the purchase intent was highest when the violins were handmade; for cups, the purchase intent was highest when machine-based manufacturing was used. Moreover, whereas the product quality perception for drones did not differ across different manufacturing methods, consumers perceived that handmade violins had the highest quality and that cups manufactured with 3D printing had the lowest quality (the purchase intent for cups was also lowest when 3D printing was used). This study is anticipated to provide a wide range of implications in various areas, including consumer psychology, marketing, and advertising.
The purpose of this study was to develope the transgenic cattle expressing hFSH into the urine using the nuclear transfer. To produce the interest gene in urine, the specific vector was ligated with hFSH gene undo. maUII promoter. The fetal fibroblast cells (KbFF) were isolated from a 45-day male fetus. The hFSH gene was co-transfected with pcDNA3 (neo) vector to KbFF cells by electroporation. The gene-transfected cells were cultured with G-418 selection medium for 2 weeks. Selected colonies were confirmed by PCR. For nuclear transfer, enucleated bovine oocytes were transferred with hFSH transfected or nontransfected fetal fibroblasts. The cleavage and blastocyst formation rates were significantly lower (p<0.05) in cloned embryos transfected with hFSH gene (68.7% and 15.7%) than in those non-transfected (67.6% and 24.5 %), respectively. Apoptosis analysis showed no difference between hFSH transfected and non-transfected blastocysts (p>0.05). The blastocysts were transfected to 77 (control 24, hFSH 53) recipient cows. Two calves were born (1.9%) following transfer with NT embryos transfected with hFSH gene, but they were confirmed not to be transgenic calves. This result shows that the hFSH colonies were mixed with transfected and non transfected cells. Further research will be needed for selection and establishment of gene transfected cells.
The present study was evaluated the antibacterial effect of the combination of $Coptidis$$rhizoma$, $Glycyrrhiza$$uralensis$ Fischet, $Schizandra$$chinensis$ and $Corni$$Fructus$(1:1:1) extracts(CGSC10). Furthermore, the effectiveness of CGSC10, sodium chlorate, and the combination of CGSC10 and sodium chlorate(CGSCS10) against $E.$$coli$ O157:H7 infection was studied using ICR female mice. During the incubation period, the dose of 5, 10, and 20% CGSC10 was inhibited the growth of $E.$$coli$ O157:H7 by 34.7, 60.2, and 76.4%, respectively. For 7 days after single challenge with $E.$$coli$ O157:H7, forty female ICR mice were divided into four experimental groups which were administered in drinking water with saline, 10% CGSC10, 15 mM sodium chlorate, and CGSCS10, respectively. On the 3rd day, the number of $E.$$coli$ O157:H7 in mouse feces was significantly decreased by administration of CGSC10, 15 mM sodium chlorate, and CGSCS10 ($p$ < 0.001). On the 7th day-after administration, CGSC10, sodium chlorate, and CGSCS10 were decreased the number of $E.$$coli$ O157:H7 by 27.1, 67.7, and 83.3%, respectively. According to the results of the present study, administration of CGSCS10 to mice can reduce the severity of $E.$$coli$ O157:H7 infection. In addition, it is suggested that CGSCS10 represents a good candidate for the treatment of enteric infections in domestic animals.
The present study was evaluated the antibacterial effect of the combination of Coptidis rhizoma, Lonicerae Flos, and Paeonia japonica (1:1:1) extracts (CLP1000). Also, the effectiveness of CLP1000, dioctahedral smectite (DHS), and the combination of CLP1000 and DHS (CLPS1000) against E. coli O157:H7 infection was studied using ICR female mice. During the incubation period, the dose of 10% and 20% CLP1000 were inhibited the growth of E. coli O157:H7 by 30% and 47%, respectively. For 7 days after single challenge with E. coli O157:H7, forty female ICR mice were divided into four experimental groups which were orally administered with saline, 10% CLP1000, 10% DHS, and 10% CLPS1000, respectively. On the 3rd day, the number of E. coli O157:H7 in mouse feces was significantly decreased by administration of CLP1000 (p < 0.05), DHS (p < 0.05) and CLPS1000 (p < 0.001). On the 7th day, CLP1000 (p < 0.05) and CLPS1000 p < 0.001) administration significantly decreased the number of E. coli O157:H7. According to the results of the present study, administration of CLPS1000 to mice can reduce the severity of E. coli O157:H7 infection. Also, it is suggested that CLPS100 represents a good candidate for the treatment of enteric infections in domestic animals.
The objective of this study was to establish a processing technology for preserved leaves based on the results from the examination of the optimal period and condition for dye-absorbing treatment for Eucalyptus cinerea F. Mull. ex Benth. (silver dollar eucalyptus) being used frequently as plant material for flower design. Cut foliages of E. cinerea with uniformly matured leaves were cut into 20 cm lengths and their lower stem parts were placed in dye solution in growth chambers with different temperatures (10, 20, 30, and $40^{\circ}C$), vapor pressure deficits (VPD; 0.23, 0.70, 1.17, and 1.61 kPa), and photoperiods (0, 6, 12, 24 hours) for 3, 6, 9, and 12 days, and then dried in a room of $20^{\circ}C$ for three days. Lower temperature during preserving dye treatment reduced the changes in leaf color compared with fresh leaves and decreased ${\Delta}E$ value. Especially, high temperature increased red degree (a) and decreased yellow degree (b) due to browning. Lower VPD reduced the change in leaf color compared with fresh leaves and decreased ${\Delta}E$ value. Shorter photoperiod reduced the change in leaf color compared with fresh leaves and decreased ${\Delta}E$ value. The ${\Delta}E$ value increased with increasing absorbing duration under three environmental conditions. The flexibility of stem and leaves after dipped into preserving dye solution and dried for 3 days increased with decreasing temperature, VPD and dipping duration. Therefore, the optimal environment condition for dye treatment was 0.23-0.70 kPa VPD at $10-20^{\circ}C$ in the darkness, and the optimal and economical duration was 3 days. These conditions reduced the speed of water loss by decreasing transpiration, so yellowing or browning by rapid water loss deteriorated the quality of preserved leaves out of these ranges.
For the past few years, KISTI has been servicing an online simulation execution platform, called EDISON, allowing users to conduct simulations on various scientific applications supplied by diverse computational science and engineering disciplines. Typically, these simulations accompany large-scale computation and accordingly produce a huge volume of output data. One critical issue arising when conducting those simulations on an online platform stems from the fact that a number of users simultaneously submit to the platform their simulation requests (or jobs) with the same (or almost unchanging) input parameters or files, resulting in charging a significant burden on the platform. In other words, the same computing jobs lead to duplicate consumption computing and storage resources at an undesirably fast pace. To overcome excessive resource usage by such identical simulation requests, in this paper we introduce a novel framework, called IceSheet, to efficiently manage simulation data based on execution metadata, that is, provenance. The IceSheet framework captures and stores each provenance associated with a conducted simulation. The collected provenance records are utilized for not only inspecting duplicate simulation requests but also performing search on existing simulation results via an open-source search engine, ElasticSearch. In particular, this paper elaborates on the core components in the IceSheet framework to support the search and reuse on the stored simulation results. We implemented as prototype the proposed framework using the engine in conjunction with the online simulation execution platform. Our evaluation of the framework was performed on the real simulation execution-provenance records collected on the platform. Once the prototyped IceSheet framework fully functions with the platform, users can quickly search for past parameter values entered into desired simulation software and receive existing results on the same input parameter values on the software if any. Therefore, we expect that the proposed framework contributes to eliminating duplicate resource consumption and significantly reducing execution time on the same requests as previously-executed simulations.
The present study was to determine how seasonal rainfall intensity influences nutrient dynamics, ionic contents, oxygen demands, and suspended solids in a lotic ecosystem. Largest seasonal variabilities in most parameters occurred during the two months of July to August and these were closely associated with large spate of rainfall. Dissolved oxygen (DO) had an inverse function of water temperature (r = = = - 0.986, p<0.001). Minimum pH values of<6.5 were observed in the late August when rainfall peaked in the study site, indicating an ionic dilution of stream water by precipitation. Electrical conductivity (EC) was greater during summer than any other seasons, so the overall conductivity values had direct correlation (r = 0.527, p<0.01) with precipitation. Ionic dilution, however, was evident 4 ${\sim}$ 5 days later in short or 1 ${\sim}$ 2 weeks in long after the intense rain, indicating a time-lag phenomenon of conductivity. Daily COD values varied from 0.8 mg $L^{-1}$ to 7.9 mg $L^{-1}$ and their seasonal pattern was similar (r = 0.548, p<0.001) to that of BOD. Total nitrogen (TN) varied little compared to total phosphorus (TP) and was minimum in the base flow of March. In contrast, major input of TP occurred during the period of summer monsoon and this pattern was similar to suspended solids, implying that TP is closely associated (r = 0.890, p<0.01) with suspended inorganic solids. Mass ratios of TN : TP were determined by TP (r= -0.509, p<0.01) rather than TN (r= -0.209, p<0.01). The N : P ratios indicated that phosphorus was a potential primary limiting nutrient for the stream productivity. Overall data suggest that rainfall intensity was considered as a primary key component regulating water chemistry in the stream and maximum variation in water quality was attributed to the largest runoff spate during the summer monsoon.
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