• Archives of Pharmacal Research
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• 제28권10호
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• pp.1164-1169
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• 2005

Brain microglia are phagocytic cells that are the major inflammatory response cells of the central nervous system and widely held to play important pathophysiologic roles in Alzheimer's disease (AD) in both potentially neurotoxic responses and potentially beneficial phagocytic responses. In the study, we examined whether ginsonoside Rg3, a by-product of red ginseng, enhances the microglial phagocytosis of $A{\beta}$. We found that Rg3 promoted $A{\beta}$ uptake, internalization, and digestion. Increased maximal $A{\beta}$ uptake was observed at 4 and 8 h after Rg3 pretreatment (25 ${\mu}g/mL$), and the internalized $A{\beta}$ was almost completely digested from cells within 36 h when pretreated with Rg3 comparing with single non-Rg3-treated groups. The expression of MSRA (type A MSR) was also up-regulated by Rg3 treatment in a dose- and time-dependent manner which was coincidently identified in western blots for MSRA proteins in cytosol. These results indicate that microglial phagocytosis of $A{\beta}$ may be enhanced by Rg3 and the effect of Rg3 on promoting clearance of $A{\beta}$ may be related to the MSRA-associated action of Rg3. Thus, stimulation of the MSRA might contribute to the therapeutic potentials of Rg3 in microglial phagocytosis and digestion in the treatment of AD.

• 한국잠사곤충학회지
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• 제41권3호
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• pp.216-221
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• 1999

This study was designed to investigate the effects of silk fibroin powder on oxygen radicals and their scavenger enzymes in serum of rats. Sprague-Dawley(SD) male rats (160${\pm}$10 g) were fed experimental diets (SFP-2.5 and SFP-5.0 groups)added 2.5 and 5.0 g/kg BW/day for 6 weeks. Triglyceride levels were remarkably inhibited (15∼25%) in SFP-2.5 and SFP-5.0 groups compared with control group. Hydroxyl radical ($.$OH) formations resulted in a marked decrease (15%∼25%) compared with control group, while superoxide radical and hydrogen peroxide formations resulted in a significant decrease (6∼12% and 10%). Lipid peroxide and oxidized protein (>C=O group) productions resulted in a significant decrease (6∼12% and 6%) compared with control group. Superoxide dismutase (SOD) and catalase (CAT) activities were remarkably increased (35∼55% and 40∼50%), but glutathione peroxidase (GSHPX) activities were significantly increased (13%) compared with control group. These results suggest that anti-aging effect of silk fibroin powder (SFP) may play a pivotal role in attenuating a various age-related changes.

• 한국잠사곤충학회지
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• 제41권3호
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• pp.141-146
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• 1999

This study was designed to investigate the effects of silkworm powder (SWP) on oxygen radicals and their scavenger enzymes in serum of rats. Sprague Dawley (SD) male rats (160${\pm}$10g) were fed experimental diets (SWP-200and SWP-400 groups)added 200 and 400mg/kg BW/day for 6 weeks. Triglyceride (TG)levels were significantly inhibited (10% and 25%) in SWP-200 and SWP-400 groups, but there were no singificant differences in total, LDL-and HDL-cholesterol levels in both SWP-200 and SWP-400 groups. Hydroxyl radical ($.$OH) formations resulted in a marked decreases (about 20%) in both SWP-200 and SWP-400 groups compared with control group. Superoxide radical (O2.-)and hydrogen peroxide formations resulted in a significant decreases in SWP-400 group compared with control group. Lipid peroxide (LPO)and oxidized protein(>C=O group) productions resulted in a significant decreases (SOD) and catalase (CAT) activiteies were remarkably increased (10∼25% and 40∼50%)in SWP-200 and SWP-400groups. Glutathione peroxidase(GSHPX) activities were significantly increased (about 10%) in SWP-400 groups compared with control group. These results suggest that anti-aging effect of silkworm powder (SWP) may play a pivotal role in attenuation a various chronic degenerative diseases age-related changes.

• Archives of Plastic Surgery
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• 제35권6호
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• pp.645-652
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• 2008

Purpose: In skin flap surgery, surgeons often encounter distal ischemia of the flap. If a powerful free radical scavenger is used, it may reduce the formation of free radical and improves the survival of flap. Thus, the present study purposed to examine whether the survival of flap can be enhanced by administering melatonin, which is known to be a powerful free radical scavenger a antioxidant molecule. Methods: We divided 40 Sprague-Dawley rats into 4 groups, 10 in each group. For the control group(n=10), we intraperitoneally injected only carrier solution once 30 minutes before the operation, and once a day for 7 days from the day of operation. Among the experimental groups, a group(n=10) was administered with dimethyl sulfoxide(DMSO), in another group(n=10), melatonin was intraperitoneally injected, and in the other(n=10) melatonin was intraperitoneally injected and applied topically(2 cc of 1% melatonin) to the operation site. Caudally based skin flaps measuring $3{\times}10cm^2$ were elevated on the mid-dorsum of the rats. and then repositioned. On the seventh postoperative day, the survival area of the flap was measured and tissues were examined under the light microscope. Results: The control group, the DMSO group, the melatonin administration group and the melatonin administration and application group showed the mean survival rates of $55.26{\pm}9.2%$, $70.29{\pm}7.47%$, $81.45{\pm}4.14%$ and $86.1{\pm}1.52%$, respectively, for $30cm^2$ of flap. Compared to the control group, the experimental groups showed a significantly high increase in survival area at significance level of 95%. Conclusion: In this study, the survival rate of flap was enhanced through the administration of melatonin after flap surgery. This suggests that melatonin not only functions as a powerful free radical scavenger and oxygen radical scavenger but also stabilizes and protects cells, and by doing so, enhances the survival of moderately injured ischemic sites in the distal end of flap.

• 한국식품과학회지
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• 제27권1호
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• pp.124-128
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• 1995

결명자 추출물을 이용하여 발암성 니트로사민의 직접적인 생성인자인 아질산염 소거작용과 그 소거인자의 분리 및 동정을 시도하였다. 결명자의 메탄올 추출물을 chloroform, ethyl ether, ethyl acetate, 물분획 순으로 순차용매 분리한 결과 ethyl acetate획분이 가장 우수한 것으로 나타났다. Ethyl acetate획분을 silica gel column chromatography하여 얻은 compound A와 B획분 중에서 compound A는 pH 1.2에서 44.5%/200 ${\mu}g$의 아질산염 소거능을, compound B는 극히 낮은 아질산염 소거능을 나타내었다. HPLC를 이용하여 compound A획분으로 부터 compound A-1획분을 분리하고 아질산염 소거효과를 ascorbic acid와 비교하였을 때 compound A-1은 ascorbic acid에 비해 월등한 소거효과를 나타내었으며, UV, IR 및 $^1H-NMR$의 결과로부터 그 구조는 $nor-rubrofusarin-6-{\beta}-mono-D-glucoside$으로 추정하였다.

• The Korean Journal of Pain
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• 제11권1호
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• pp.1-6
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• 1998

Background: The role of nitric oxide(NO) in analgesia from opioids is controversial. On the one hand, IV morphine analgesia is enhanced by IV injection of NO synthase inhibitors. On the other hand, IV morphine results in increased release of NO in the spinal cord. There have been no behavioral studies examining the interaction between IV morphine and intrathecal injection of drugs which affect NO synthesis. Method: Rats were prepared with chronic lumbar intrathecal catheters and were tested withdrawal latency on the hot plate after 3~5 days of surgery. Antinociception was determinined in response to a heat stimulus to the hind paw before and after IV injection of morphine, 2.5 mg/kg. Twenty minutes after morphine injection, rats received intrathecal injection of saline or the NO synthase inhibitors, L-NMMA or TRIM, the NO scavenger, PTIO, or the NO synthase substrate, L-Arginine. Intrathecal injections, separated by 15 min, were made in each rats and measurements were obtained every 5 min. Result: Mophine produced a 60~70% maximal antinociceptive response to a heat stimulus in all animals for 60 min in control experiments. Intrathecal injection of idazoxane decreased antinociception of IV morphine. The NO synthase inhibitors and the NO scavenger produced dose-dependent decreases in antinociceptive effect of morphine, whereas saline as a control group and L-Arginine as the NO substrate had no effect on antinociception of morphine. Conclusion: The present study supports the evidences that systemic morphine increase the nitrite in cerebrospinal fluid and dorsal horn. These data suggest that the synthesis of NO in the spinal cord may be important to the analgesic effect of IV morphine and increased NO in spinal cord has different action from the supraspinal NO.

• Journal of the Korean Wood Science and Technology
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• 제22권1호
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• pp.44-53
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• 1994

This research was carried out to investigate the effect of paper sludge addition on formaldehyde emission, and physical and mechanical properties of UF-particleboard. In order to investigate the effect of paper sludge addition to resin, particleboards were bonded with urea-formaldehyde resins containing 5, 10, 15% paper sludge powders of three types(A Type: -200 mesh, B Type: -100~+200 mesh. C Type: -50~+100 mesh), based on weight of resin solid. Also the effect of paper sludge addition to furnish was studied from particleboards fabricated with ratios of sludge to particle of 5:95, 10:90, 15:85 based on oven-dry weight. Tests were conducted on the manufactured particleboards to determine formaldehyde emission, bending properties, internal bond strength and thickness swelling. The obtained results were summarized as follows: The addition of paper sludge powder to resin yielded a higher pH of cured resin. Formaldehyde emission decreased with the increase of paper sludge powder addition to resin and paper sludge composition ratio to furnish. Particleboard bonded with urea-formaldehyde resin containing paper sludge powder and particleboard mixed with paper sludge have similar bending properties(MOR, MOE) and thickness swelling compared with control particleboard. Internal bond strength of particleboards treated with paper sludge were lower than that of control particleboard. The use of paper sludge as scavenger was achieved reduction of formaldehyde emission without depression of physical and mechanical properties of particleboard. Also the use of paper sludge was able to concluded that there is possibility of partial substitution of wood particle materials.

• 한국식품영양과학회지
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• 제22권5호
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• pp.570-575
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• 1993

양조간장 분말을 Sephadex 겔 여과 크로마토그래피법으로 분획한 후, 가장 갈색도가 높은 melanoidin 관련 물질의 획분인 melanoidin related products(MRPs)을 모아 동결 건조시킨 다음, 이의 free redical scavenger 기능, 항산화 효력상승제의 기능과 금속 킬레이터로서의 기능, 리폭시게나아제 활성 저해능, 그리고 저장안전성 등에 대한 항산화 특성을 고찰한 결과 다음과 같다. MRPs는 첨가농도(혹은 갈색도)의 증가에 따라 과산화물의 생성을 저해하였으며, 그 항산화력은 ${\alpha}-토코페롤$ 보다는 낮았다. 그리고 free redical scavenger로서의 능력을 가지고 있어 리놀레산 혼합물의 산호를 지연 시켰으며, 염화제일철에 의하여 촉매된 산화반응에서 synergistic antioxidative effect를 나타내었다. 또한 양조간장 MRPs는 리폭시게나아제 활성을 저해하여 conjugated dienoic acid 생성량을 감소시켰다. 한편, 이 MRPs는 저장 안전성이 상당히 있어, $50^{\circ}C$에서 15일간 저장되어 있어도 항산화 효과의 상실이 거의 없음이 관찰되었다.

• 대한의생명과학회지
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• 제12권4호
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• pp.451-455
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• 2006

It is demonstrated that inorganic mercury has cytotoxic effect on glial cells. Recently, oxygen radicals is involved in methylmercuric chloride (MMC)-induced cytotoxicity. But, the toxic mechanism of MMC is left unknown. The purpose of this study was to examine the cytotoxicity of MMC on $C_6$ glioma cells. The cytotoxicy was measured by cell viability using XTT assay in $C_6$ glioma cells. Colorimetric assay is regarded as a very sensitive screening method for the determination of the cell viability on various agents. In this study, MMC decreased cell viability according to the dose- and time dependent manners after $C_6$ glioma cells were grown with various concentrations of MMC for 48 hours. In the protective effect of allopurinol on MMC-induced cytotoxicity, allopurinol was effective in the prevention of MMC-induced cytotoxicity in these cultures. These results suggest that MMC has highly cytotoxic effect on $C_6$ glioma cells by the decrease of cell viavility, and free radical scavenger such as allopurinol was effective on organic mercury-induced cytotoxicity in these cultures.

• 한국산학기술학회논문지
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• 제20권8호
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• pp.477-484
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• 2019

포유류에 존재하는 Collectin-Placenta 1 (CL-P1)을 포함한 여러 종류의 scavenger 수용체는 주로 내피 세포, 대식 세포 및 평활근 세포 표면에 발현되는 분자이다. 이들 분자는 산화 된 저밀도 지질 단백질 (oxLDL)에 결합하여 처리 할 수 있는 세포 표면 당 단백질이다. 이들 분자 중 케르세틴이 CL-P1 활성화에 어떤 영향을 미치는가를 확인하였다. 케르세틴은 산화 반응을 담당하는 자유 라디칼의 제거제 역할을 하여 산화를 중지시키는 항산화제로 알려져 있다. 본 논문에서는 마우스 CL-P1 유전자 promoter 부분의 전사 시작 점부터 -500 번째 염기까지의 단편을 DNA 중합효소를 이용하여 클로닝 하였다. 그 후에 대식세포 계열인 RAW264.7 및 섬유아세포계열의 NIH3T3 세포에 도입하여 케르세틴이 CL-P1 유전자 발현에 어떠한 영향을 미치는지에 대한 연구를 하였다. 이 부위에는 세포주기 조절 인자인 E2F 결합부위를 비롯해서 여러 종류의 전사 인자가 결합하는 염기서열이 다수 위치하고 있다. 이러한 500염기 단편을 pGL4.10 기본 벡터 및 프로모터에 연결시킨 후 세포에 도입시켰다. 그리고 배양 중에 케르세틴을 처리하여 유전자 발현양을 형광 색소 발현기법으로 측정하였다. 그 결과 유전자 발현이 시작되는 앞쪽 부분의 -250에서 -350사이의 염기들이 CL-P1 단백질을 만드는데 중요하다는 것을 확인하였다. 그 중에서도 E2F결합 부위가 결정적인 것 이라는 것을 DNA 돌연변이 실험을 통해 확인 하였다. 또한 부착 세포인 RAW264.7 배양액에 케르세틴을 첨가 한 결과, 배양용기 표면에서 탈락하는 현상을 확인하였다. 즉 발현된 CL-P1단백질이 케르세틴에 의해 세표 표면의 부착 분자에도 영향을 주는 것을 확인하였다.