• 환경영향평가
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• 제22권2호
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• pp.183-194
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• 2013

The Ministry of Environment, Ministry of Agriculture, Fishery and Forestry, and Ministry of Construction, Transportation and Maritime Affairs are in charge of livestock manure management. There are national statistics regarding the livestock industry such as the National Pollution Source Survey, Livestock Statistic Survey, and Livestock-breeding Trend Survey. The current statistical data are focused on the scale of livestock breeding and the production of livestock manure using these data, but it is difficult to establish database due to lack of information. In order to plan relevant policies including management of livestock manure, the government established database systems such as the integrated information system of livestock manure, the integrated system of national infectious animal-disease prevention, and the Sae-ol public administrative system. We have tried to suggest improvements for the comprehensive environmental information system of livestock manure management by detecting problems in each level of the livestock manure life-cycle, making use of the existing systems, and considering the electronic transfer system of livestock manure. The services and functions of this comprehensive system include information of livestock farmers, the production, collection, transportation, and treatment of livestock manure, the area of agricultural land used for livestock manure, the report of approval and results on livestock manure products, management of statistical information, management of civil affairs, and relevant mobile application services. The system is made up of three processes: first, establishment of GIS-based management database of livestock manure; second, establishment of a history management system for livestock manure transactions; and third, development of a water quality assessment system.

• KSBB Journal
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• 제30권6호
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• pp.307-312
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• 2015

Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and $k_La$. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.

• 농촌계획
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• 제12권1호
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• pp.37-48
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• 2006

The objectives of this study were to develop an evaluation method of regional vulnerability to agricultural drought and to classify the vulnerability patterns. In order to test the method, 24 city or county areas of Gyeonggi-do were chose. First, statistic data and digital maps referred for agricultural drought were defined, and the input data of 31 items were set up from 5 categories: land use factor, water resource factor, climate factor, topographic and soil factor, and agricultural production foundation factor. Second, for simplification of the factors, principal component analysis was carried out, and eventually 4 principal components which explain about 80.8% of total variance were extracted. Each of the principal components was explained into the vulnerability components of scale factor, geographical factor, weather factor and agricultural production foundation factor. Next, DVIP (Drought Vulnerability Index for Paddy), was calculated using factor scores from principal components. Last, by means of statistical cluster analysis on the DVIP, the study area was classified as 5 patterns from A to E. The cluster A corresponds to the area where the agricultural industry is insignificant and the agricultural foundation is little equipped, and the cluster B includes typical agricultural areas where the cultivation areas are large but irrigation facilities are still insufficient. As for the cluster C, the corresponding areas are vulnerable to the climate change, and the D cluster applies to the area with extensive forests and high elevation farmlands. The last cluster I indicates the areas where the farmlands are small but most of them are irrigated as much.

• Journal of Dairy Science and Biotechnology
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• 제25권2호
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• pp.33-40
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• 2007

The Korean dairy industry has been developed continuously for the past seventy years both in quality and quantity; now the total amount of dairy products accounts for 13 percent of that of livestock production - a remarkable turnover. Korea now stands at the 13th economic power around the world and it is inevitable that we must pursue an open-door policy at a global level; the dairy industry should also strengthen its potentialities in the long term in order to compete with advanced dairy countries. It is predicted that the impact of DDA agreement and FTA negotiations on the domestic dairy industry will be great; so it is urgently needed that we should reconsider the current status of the dairy industry and comprehensive mesures for dairy industry development to improve its international competitiveness. Such measures comprise the three aspects: first, reforming the policy of dairy industry-related systems, second, improving the productivity and third, expanding the consumption of dairy products. In terms of the system reforming, the most important things are, above all, the planned production relevant to market needs, the milk supply and demand management, and the raw milk transaction method. Secondly, the appropriate scale of management is important for the enhancement of productivity together with the modernization of dairy facilities. Thirdly, step-up effort is needed to expand the milk consumption; especially, more milk should be provided to the students by improving the standing milk ration system in middle and senior high schools. The government should make more effort to support and develop new dairy products tuned to consumers' taste, accelerating the publicity of diverse and steady milk consumption together with related organizations.

• 원예과학기술지
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• 제30권2호
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• pp.137-143
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• 2012

Chinese cabbage plant was grown hydroponically for 4 weeks in order to examine the temporal relationship of calcium concentration of the nutrient solution with calcium content in the leaf tissue and susceptibility of the tissue to soft rot disease by $Pectobacterium$ $carotovorum$ ssp. $carotovorum$ (Pcc). Calcium concentration from 0.5 to 32.0 mM was maintained for 1 week using Hoagland & Arnon solution. The calcium content of the leaf was proportionally increased to the concentration of the nutrient in the solution (r = 0.912). In contrast, the severity of soft rot symptom in the young leaves was inversely related with the amount of calcium supplied to the nutrient solution (r = 0.899). Water-soluble chitosan, prepared by hollow fiber filtration (> 100 kDa) was applied into the nutrient solution from 0.0 to 5,000 ppm. The chitosan of 10 ppm was the most effective to promote calcium uptake of the leaf, showing 155% of the control. The same chitosan solution prohibited most soft rot development of the leaf by Pcc, exhibiting only 53% of the control. Among different molecular weight fractions, chitosan fraction obtained from 30-100 kDa molecular weight cut-off promoted calcium uptake the most up to 163% of the control, and reduced the development of soft rot disease recording merely 36% of the control of the leaf tissue. The results obtained in the present study suggest that large scale production of water-soluble chitosan with an optimum molecular weight and its commercial application to Chinese cabbage production will be important to improve yield and quality of the crop.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.153-159
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• 2008

The limitations in current technology for generating transgenic animals, such as the time and the expense, hampered its extensive use in recombinant protein production for therapeutic purpose. In this report, we present a simple and less expensive alternative by directly infusing a recombinant adenovirus vector carrying human lactoferrin cDNA into rabbit mammary glands. The milk serum was collected from the infected mammary gland 48 h post-infection and subjected to a 10% SDS-PAGE and Western blotting. An 80-kDa protein was visualized after viral vector infection. With this method, we obtained a high level of expressed human lactoferrin of up to 2.3mg/ml in the milk. Taken together, the method is useful for the transient high-level expression recombinant proteins, and the approach established here is probably one of the most economical and efficient ways for large-scale production of recombinant proteins of biopharmaceutical interest.

• 펄프종이기술
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• 제47권2호
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• pp.61-69
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• 2015

A number of researches have been carried out regarding the utilization of nanocellulose(crystalline nanocellulose, microfibrillated cellulose, nanofibrillated cellulose) for the manufacture of various kinds of composites and functional products. However, only few research works on the manufacturing characteristics of nanocellulose could be found, although some companies started already the production of nanocellulose in commercial scale. However, the most important thing in commercializing of production and utilization of nanocellulose is to develop the economical and efficient process. Thus, this study was carried out in order to investigate the effects of refining, alkaline pre-treatment and grinder clearance on the characteristics of microfibrillated cellulose and energy consumption. There was no significant differences in crystalline index with the degree of microfibrillation. The initial fibrillation could be improved by refining pre-treatment, but its effect was not observed anymore since the fibrillation was done up to certain level by grinding. Refining pre-treatment did not improved the energy efficiency. Alkaline pre-treatment can be helpful because the swelling of pulp fiber will facilitate fibrillation. It was found that the decrease in grinder clearance was helpful to improve the energy efficiency.

• 한국축산식품학회지
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• 제28권1호
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• pp.1-8
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• 2008

최근까지 박테리오신 생산균주의 분리, 분자생물학적 메커니즘, 정제, 구조 및 작용기작, 산업적인 적용 등의 논문 등이 보고되고 있다. 또한 50개국 이상에서 치즈, 통조림식품 등에서 식품보존제로서 승인되어 있다. 박테리오신의 여러 장점으로 인해 현재 추진되고 있는 축산업 분야의 HACCP의 범위인, 사육장에서부터 식품제조에 이르기까지 항생물질, 인공적인 방부제나 식품첨가물이 적게 들어가거나 첨가되지 않은 자연식품을 선호하고 있는 실정에 적합하다. 향후 축산업에서 항생제 대체방안의 하나로서 박테리오신의 사용은 확대되리라 기대된다. 경제적인 가격 경쟁력을 갖기 위해, 대량생산에 대한 연구가 이루어져야 하며, 적절한 제형으로 보다 구체적인 적용실험이 수행되어 산업화를 앞당겨야 한다.

• 한국전자통신학회논문지
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• 제15권2호
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• pp.225-230
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• 2020

산업혁명의 발전으로 소품종 대량생산에서 다품종 소량 생산의 형태로 제조의 패러다임이 변화하고 있음을 확인할 수 있는데, 이는 생산자의 무분별한 제고의 부담을 줄일 수 있는 형태로 현대 사회가 발전할수록 바뀌어야 하는 생산성 부분의 문제점을 보완하려는 산업형태의 변화를 보여주고 있다. 3D Printing 환경에서 살펴보면 프린팅의 마지막 단계까지 도달하는 과정에서 많은 문제가 발생한다. 이 문제는 저가의 보급형 3D Pirnter 만이 아니라 완제품으로 출시되는 고가의 3D Printer 또한 같은 증상들이 나타나고 있으며 프린팅을 하는 과정에서 불안요소를 발생시킬 수 있다. 그래서 이와 같은 문제점을 보완하고자 본 연구에서는 3D Printer의 안전성 및 제품 출력물에 대한 품질 관리 시스템에 대한 제안을 연구에 기초하여 3D Printer 개인 사용자를 타겟으로 하는 IoT 비콘 기반 3D Printer 원격 안전·품질 관리 시스템 설계를 제안한다.

• International Journal of Stem Cells
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• 제9권2호
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• pp.186-191
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• 2016

Background and Objectives: With the global demand for dairy protein for consumption growing annually, there has been increasing activity in the research field of dairy protein synthesis and production. From a manipulation perspective, it is more difficult to use live cattle for laboratory studies on the production of milk as well as of dairy protein such as casein, as compared with using laboratory animals like rodents. Therefore, we aimed to develop a mouse model of bovine mammary alveolar ducts for laboratory-scale studies. We studied the formation of the bovine mammary gland ductal structure by transplanting the MAC-T bovine alveolar cell line into mice. Methods and Results: MAC-T cells ($1{\times}10^7$) were suspended in Matrigel and injected into the dorsal tissue of 8-week-old male BALB/C nude mice. Histological analysis of tissue dissected from the MAC-T cell-transplanted mice after 6 weeks showed the typical morphology of the tubuloalveolar female gland, as well as glands made up of branching ducts that were surrounded by smooth muscle with small alveoli budding off the ducts. In addition, the epithelial markers CK14 and CK18 were expressed within the duct-like structure. Prolactin was detected in the duct interior in these CK14+ and CK18+ cells but not in the non-transplanted MAC-T cells. Conclusions: These results showed that duct-like tissue had been successfully formed after 6 weeks of transplantation of the CK14+ and CK18+ MAC-T cells into mice dorsal tissue. This mouse model will be a useful tool for further research on the bovine mammary gland.