• Journal of Environmental Impact Assessment
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• v.22 no.2
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• pp.183-194
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• 2013

The Ministry of Environment, Ministry of Agriculture, Fishery and Forestry, and Ministry of Construction, Transportation and Maritime Affairs are in charge of livestock manure management. There are national statistics regarding the livestock industry such as the National Pollution Source Survey, Livestock Statistic Survey, and Livestock-breeding Trend Survey. The current statistical data are focused on the scale of livestock breeding and the production of livestock manure using these data, but it is difficult to establish database due to lack of information. In order to plan relevant policies including management of livestock manure, the government established database systems such as the integrated information system of livestock manure, the integrated system of national infectious animal-disease prevention, and the Sae-ol public administrative system. We have tried to suggest improvements for the comprehensive environmental information system of livestock manure management by detecting problems in each level of the livestock manure life-cycle, making use of the existing systems, and considering the electronic transfer system of livestock manure. The services and functions of this comprehensive system include information of livestock farmers, the production, collection, transportation, and treatment of livestock manure, the area of agricultural land used for livestock manure, the report of approval and results on livestock manure products, management of statistical information, management of civil affairs, and relevant mobile application services. The system is made up of three processes: first, establishment of GIS-based management database of livestock manure; second, establishment of a history management system for livestock manure transactions; and third, development of a water quality assessment system.

• KSBB Journal
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• v.30 no.6
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• pp.307-312
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• 2015

Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and $k_La$. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.

• Journal of Korean Society of Rural Planning
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• v.12 no.1 s.30
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• pp.37-48
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• 2006

The objectives of this study were to develop an evaluation method of regional vulnerability to agricultural drought and to classify the vulnerability patterns. In order to test the method, 24 city or county areas of Gyeonggi-do were chose. First, statistic data and digital maps referred for agricultural drought were defined, and the input data of 31 items were set up from 5 categories: land use factor, water resource factor, climate factor, topographic and soil factor, and agricultural production foundation factor. Second, for simplification of the factors, principal component analysis was carried out, and eventually 4 principal components which explain about 80.8% of total variance were extracted. Each of the principal components was explained into the vulnerability components of scale factor, geographical factor, weather factor and agricultural production foundation factor. Next, DVIP (Drought Vulnerability Index for Paddy), was calculated using factor scores from principal components. Last, by means of statistical cluster analysis on the DVIP, the study area was classified as 5 patterns from A to E. The cluster A corresponds to the area where the agricultural industry is insignificant and the agricultural foundation is little equipped, and the cluster B includes typical agricultural areas where the cultivation areas are large but irrigation facilities are still insufficient. As for the cluster C, the corresponding areas are vulnerable to the climate change, and the D cluster applies to the area with extensive forests and high elevation farmlands. The last cluster I indicates the areas where the farmlands are small but most of them are irrigated as much.

• Journal of Dairy Science and Biotechnology
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• v.25 no.2
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• pp.33-40
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• 2007

The Korean dairy industry has been developed continuously for the past seventy years both in quality and quantity; now the total amount of dairy products accounts for 13 percent of that of livestock production - a remarkable turnover. Korea now stands at the 13th economic power around the world and it is inevitable that we must pursue an open-door policy at a global level; the dairy industry should also strengthen its potentialities in the long term in order to compete with advanced dairy countries. It is predicted that the impact of DDA agreement and FTA negotiations on the domestic dairy industry will be great; so it is urgently needed that we should reconsider the current status of the dairy industry and comprehensive mesures for dairy industry development to improve its international competitiveness. Such measures comprise the three aspects: first, reforming the policy of dairy industry-related systems, second, improving the productivity and third, expanding the consumption of dairy products. In terms of the system reforming, the most important things are, above all, the planned production relevant to market needs, the milk supply and demand management, and the raw milk transaction method. Secondly, the appropriate scale of management is important for the enhancement of productivity together with the modernization of dairy facilities. Thirdly, step-up effort is needed to expand the milk consumption; especially, more milk should be provided to the students by improving the standing milk ration system in middle and senior high schools. The government should make more effort to support and develop new dairy products tuned to consumers' taste, accelerating the publicity of diverse and steady milk consumption together with related organizations.

• Horticultural Science & Technology
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• v.30 no.2
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• pp.137-143
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• 2012

Chinese cabbage plant was grown hydroponically for 4 weeks in order to examine the temporal relationship of calcium concentration of the nutrient solution with calcium content in the leaf tissue and susceptibility of the tissue to soft rot disease by $Pectobacterium$ $carotovorum$ ssp. $carotovorum$ (Pcc). Calcium concentration from 0.5 to 32.0 mM was maintained for 1 week using Hoagland & Arnon solution. The calcium content of the leaf was proportionally increased to the concentration of the nutrient in the solution (r = 0.912). In contrast, the severity of soft rot symptom in the young leaves was inversely related with the amount of calcium supplied to the nutrient solution (r = 0.899). Water-soluble chitosan, prepared by hollow fiber filtration (> 100 kDa) was applied into the nutrient solution from 0.0 to 5,000 ppm. The chitosan of 10 ppm was the most effective to promote calcium uptake of the leaf, showing 155% of the control. The same chitosan solution prohibited most soft rot development of the leaf by Pcc, exhibiting only 53% of the control. Among different molecular weight fractions, chitosan fraction obtained from 30-100 kDa molecular weight cut-off promoted calcium uptake the most up to 163% of the control, and reduced the development of soft rot disease recording merely 36% of the control of the leaf tissue. The results obtained in the present study suggest that large scale production of water-soluble chitosan with an optimum molecular weight and its commercial application to Chinese cabbage production will be important to improve yield and quality of the crop.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.153-159
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• 2008

The limitations in current technology for generating transgenic animals, such as the time and the expense, hampered its extensive use in recombinant protein production for therapeutic purpose. In this report, we present a simple and less expensive alternative by directly infusing a recombinant adenovirus vector carrying human lactoferrin cDNA into rabbit mammary glands. The milk serum was collected from the infected mammary gland 48 h post-infection and subjected to a 10% SDS-PAGE and Western blotting. An 80-kDa protein was visualized after viral vector infection. With this method, we obtained a high level of expressed human lactoferrin of up to 2.3mg/ml in the milk. Taken together, the method is useful for the transient high-level expression recombinant proteins, and the approach established here is probably one of the most economical and efficient ways for large-scale production of recombinant proteins of biopharmaceutical interest.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.47 no.2
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• pp.61-69
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• 2015

A number of researches have been carried out regarding the utilization of nanocellulose(crystalline nanocellulose, microfibrillated cellulose, nanofibrillated cellulose) for the manufacture of various kinds of composites and functional products. However, only few research works on the manufacturing characteristics of nanocellulose could be found, although some companies started already the production of nanocellulose in commercial scale. However, the most important thing in commercializing of production and utilization of nanocellulose is to develop the economical and efficient process. Thus, this study was carried out in order to investigate the effects of refining, alkaline pre-treatment and grinder clearance on the characteristics of microfibrillated cellulose and energy consumption. There was no significant differences in crystalline index with the degree of microfibrillation. The initial fibrillation could be improved by refining pre-treatment, but its effect was not observed anymore since the fibrillation was done up to certain level by grinding. Refining pre-treatment did not improved the energy efficiency. Alkaline pre-treatment can be helpful because the swelling of pulp fiber will facilitate fibrillation. It was found that the decrease in grinder clearance was helpful to improve the energy efficiency.

• Food Science of Animal Resources
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• v.28 no.1
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• pp.1-8
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• 2008

More safe and natural food was recently needed by consumers. Antimicrobials including sodium azide, penicillin, and vancomycin were used for therapeutic agents against pathogens such as Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7 in dairy and meat industry. These antimicrobials and preservatives were prohibited in stock farm and food because they were caused resistant strain and side effects. Bacteriocins are proteinaceous compounds that may present antimicrobial activity towards important food-borne pathogens and spoilage-related microflora. Therefore, bacteriocins were reported as an alternative of antimicrobials. Due to these properties, bacteriocin-producing strains or purified bacteriocins have a great potential of use in biologically based food preservation systems. Despite the growing number of articles regarding on the isolation of bacteriocinogenic strains, genetic determinants for production, purification and biochemical characterization of these inhibitory substances, there are only limited reports of successful application of bacteriocins to dairy and meats. This review describes bacteriocins related to dairy and meat products for the further use.

• The Journal of the Korea institute of electronic communication sciences
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• v.15 no.2
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• pp.225-230
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• 2020

The development of the industrial revolution can confirm the paradigm of manufacturing is changing from the mass production of small varieties to the form of small-scale production of many varieties. That is a form that can reduce the burden of indiscriminate raising of producers and a change in the industrial form that seeks to make up for the problems of the productivity portion that should change as modern society develops. Looking at the 3D printing environment, there are many problems in reaching the final stage of printing. The problem is not just the low-cost, low-end 3D Pirnter, but the high-priced 3D Printers released as finished products also show the same symptoms. This can cause anxiety factors in the printing process. Therefore, the research was conducted to compensate such problems. Based on the research, we propose the design of IoT beacon-based 3D Printer remote safety and quality control system targeting 3D Printer individual users.

• International Journal of Stem Cells
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• v.9 no.2
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• pp.186-191
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• 2016

Background and Objectives: With the global demand for dairy protein for consumption growing annually, there has been increasing activity in the research field of dairy protein synthesis and production. From a manipulation perspective, it is more difficult to use live cattle for laboratory studies on the production of milk as well as of dairy protein such as casein, as compared with using laboratory animals like rodents. Therefore, we aimed to develop a mouse model of bovine mammary alveolar ducts for laboratory-scale studies. We studied the formation of the bovine mammary gland ductal structure by transplanting the MAC-T bovine alveolar cell line into mice. Methods and Results: MAC-T cells ($1{\times}10^7$) were suspended in Matrigel and injected into the dorsal tissue of 8-week-old male BALB/C nude mice. Histological analysis of tissue dissected from the MAC-T cell-transplanted mice after 6 weeks showed the typical morphology of the tubuloalveolar female gland, as well as glands made up of branching ducts that were surrounded by smooth muscle with small alveoli budding off the ducts. In addition, the epithelial markers CK14 and CK18 were expressed within the duct-like structure. Prolactin was detected in the duct interior in these CK14+ and CK18+ cells but not in the non-transplanted MAC-T cells. Conclusions: These results showed that duct-like tissue had been successfully formed after 6 weeks of transplantation of the CK14+ and CK18+ MAC-T cells into mice dorsal tissue. This mouse model will be a useful tool for further research on the bovine mammary gland.