• Journal of Nutrition and Health
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• 제51권6호
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• pp.507-514
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• 2018

본 연구에서는 모자반목 모자반과 갈조류인 괭생이 모자반의 3가지 서로 다른 추출물의 면역활성 가능성을 비교연구하기 위하여 RWA264.7 대식세포에 서로 다른 괭생이 모자반 추출물을 처리하여 $TNF-{\alpha}$, IL-6 및 NO 분비능과 MAPK 신호전달경로 및 $NF-{\kappa}B$ 활성화 수준을 측정하였다. 괭생이 모자반 열수추출물, 주정추출물, 그리고 초임계추출물 중 괭생이 모자반 열수추출물이 세포독성은 없는 농도 ($50{\mu}g/mL$)에서 $TNF-{\alpha}$, IL-6의 생성뿐 아니라 iNOS 활성화를 통한 NO 분비를 촉진하였다. 이러한 괭생이 모자반 열수추출물의 면역증진 효과는 MAPK 신호전달경로 및 $NF-{\kappa}B$ 활성화를 통해 이루어지고 있음을 확인하였다. 본 연구결과는 괭생이 모자반 열수추출물의 면역활성 증진 가능성을 제시하여 향후 대식세포의 활성 유도를 통한 면역증진 치료에 유용하게 사용될 수 있음을 보여주는 것이다.

• 한국수산과학회지
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• 제1권2호
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• pp.121-127
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• 1968

This research has been dealt with the nutritional component analysis of two kinds of non-edible marine algae, Sargassum herneri (Turner) C. Ag. and Zostera marina Linne which grow abundantly around the southern coast of Korea, These marine algae were mixed in several kinds of samples rates with the combined feed for poultry sold in the market. These were given to 35 chicken in seven test divisions respectively. We have experimented with 35 chicken grown up for two weeks after hatchout. The combined food for poultry obtained from the market was set up as control divisions. The experiments were as follows: 1) The average weight increase in each test division during feeding (Table 5, Fig.2). 2) Food conversion rate and food efficiency in each test division (Table 7). 3) The comparison of digestive rate of crude protein during feeding (Table 8), The results were as follows: 1) The weight increasing rate of the test animal stock fed the food containing $5\~10\%$ of Sargassum horneri (Turner) C. Ag. powder was higher than the rate of those fed only market food for poultry. 2) The stock given food containing $10\%$ Zostera marine powder showed lower growth than the control divison. 3) No apparent trouble owing to salt component involved in the marine algae was found. 4) The stock given food containing sodium glutaminate and Sargassum horneri (Turner) C. Ag. had better result than that without sodium glutaminate.

• ALGAE
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• 제22권3호
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• pp.201-207
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• 2007

For getting primary cue of restoration, the algal flora and intertidal community structure of Deokjeokdo, Yeonpyeongdo and Ganghwado were investigated for from February to November 2006. The total of 28 species, including five Chlorophytes, five Phaeophytes, 17 Rhodophytes and one sea grass were identified. The occurrence of species according to sampling site was 22 species in Deokjeokdo, seven species in Yeonpyeongdo and four species in Ganghwado. Among them Gloiopeltis furcata, Ulva pertusa and Sargassum horneri in Deokjeokdo, Scytosiphon lomentaria in Yeonpyeongdo, Enteromorpha prolifera in Ganghwado were ominants. The algal zonation of intertidal zone was figured out by Gloiopeltis furcata, Caulacanthus okamurae – Corallina pilulifera – Ulva pertusa, Ahnfeltiopsis flabelliformis, Sargassum horneri, Undaria pinnatifida in Deokjeokdo, Bangia atropurpurea – Scytosiphon lomentaria – Ahnfeltiopsis flablleiformis in Yeonpyeongdo from upper to lower zone and Enteromorpha prolifera, Enteromorpha linza – Sargassum thunbergii, Myelophycus simplex in Ganghwado from middle to lower zone. The average of biomass was measured as 80.6 g dry wt. m–2 in Deokjeokdo, 32.2 g dry wt. m–2 in Yeonpyeongdo and less than 1 g dry wt. m–2 in Ganghwado.

• 해양환경안전학회지
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• 제15권2호
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• pp.99-104
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• 2009

진해만 조간대에서 서식하는 해조류 군집 구조를 파악하기 위하여 2007년에 8개 정점을 대상으로 계절별로 조사하였다. 조사기간 동안 총 45종의 해조류가 서식하였으며, 이 중 녹조류 4종, 갈조류 10종, 그리고 홍조류가 29종으로 나타났다. 우점종은 Ulva pertusa, U. conglobata, Sargassum horneri, Gelidium divaricatum, Gracilaria textorii, Polysiphonia morrowii으로 나타났다. 출현종수는 계절 및 정점에 따라 변하였다. 겨울에 가장 많은 42종이 출현하였으며, 여름에는 33종으로 가장 적었다. 정점별로는 정점 1,7,8에서 가장 많았으며, 정점 2와 3에서 가장 낮게 나타났나. 해조류 군집의 공간적 분포를 파악하기 위한 MDS 분석 결과에서는 해조류의 종조성 및 현존량이 각 정점의 물리해양학적인 특징 및 지형적인 차이에 의한 것으로 나타났나.

• Journal of Microbiology and Biotechnology
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• 제33권3호
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• pp.363-370
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• 2023

Atopic dermatitis (AD) is a chronic inflammation associated with skin hypersensitivity caused by environmental factors. The objent of this study was to assess the hot water extracts of Sargassum horneri (SHHWE) on AD. AD was induced by spreading 2,4-dinitrochlorobenzene (DNCB) on the BALB/c mice. The efficacy of SHHWE was tested by observing the immunoglobulin E (IgE), cytokine, skin clinical severity score and cytokine secretions in concanavalin A (Con A)-stimulated splenocytes. The levels of interleukine (IL)-4, IL-5 and IgE, the pro-inflammatory cytokines that are closely related, were notably suppressed in a does-dependent manner by SHHWE, whereas the level of interferon γ (IFN-γ), the atopy-related Th1 cytokine inhibiting the production of Th2 cytokines, was increased. Therefore, these results show that SHHWE has a potent anti- inhibitory effect on AD and is highly valuable for cosmetic development.

• 생명과학회지
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• 제29권2호
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• pp.209-214
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• 2019

본 연구에서는 괭생이모자반 에탄올 추출물의 항비만 효과에 대해 알아보고자 3T3-L1 preadipocyte에서 Oil Red O staining과 triglyceride content, adipogenesis의 발현에 대해 조사하였다. MTT assay를 통해 괭생이모자반 에탄올 추출물의 세포 독성을 확인 해 본 결과 $200{\sim}1,000{\mu}g/ml$ 농도에서 90% 이상의 세포 생존율을 확인 할 수 있었다. 또한 insulin, dexamethasone과 IBMX 복합물을 처리하여 분화를 유도하였을 때는 분화유도제를 처리하지 않은 군에 비해 활발하게 지방구를 형성하였으나, 괭생이모자반 에탄올 추출물을 250, 500, $1,000{\mu}g/ml$ 농도로 처리하였을 때는 농도 의존적으로 지방구 형성을 억제하였다. 이를 정량하여 triglyceride content (%)로 나타낸 결과 농도별로 13%, 16%, 23% 억제 효과를 나타내었다. 또한 Western blot assay를 통해 adipogenic transcription factor의 발현양상을 비교해 본 결과, 분화유도제 처리에 의해 $C/EBP{\alpha}$, $C/EBP{\beta}$와 $PPAR{\gamma}$의 발현이 증가하였으나, 괭생이모자반 에탄올 추출물을 처리하였을 때는 이러한 전사인자들의 발현이 농도 의존적으로 감소됨을 확인 할 수 있었다. 이상의 결과를 통해 괭생이모자반 에탄올 추출물은 adipogenic transcription factor의 발현을 조절하여 지방세포의 분화를 억제하였으며, 지방구와 triglyceride 생성을 감소시켜 최종적으로 비만억제 효과를 나타낸다는 것을 알 수 있었다.

• Journal of Plant Biology
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• 제38권2호
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• pp.153-158
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• 1995

Community structure of subtidal macroalgae was investigated at 3 sites of Neobdo Island in August 1994. In total, 66 taxa of red, 21 brown, 14 green, and 2 blue-green algae were identified. The number of species and biomass decreased with increasing depths. Gelidium amansii, Ulva pertusa, Dictyota dichotoma, Sargassum thunbergii, Undaria pinnatifida, Ecklonia cava, and Sargassum horneri had higher frequency and biomass. The vegetation of deeper zone was composed mainly of a few red algae. Substrate characteristic and light intensity were considered as environmental factors determining the underwater vegetation.

• ALGAE
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• 제31권3호
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• pp.243-256
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• 2016

Concerns about how ocean acidification will impact marine organisms have steadily increased in recent years, but there is a lack of knowledge on the responses of macroalgae. Here, we adopt an outdoor continuous-flowing mesocosm system designed for ocean acidification experiment that allows high CO₂ conditions to vary with natural fluctuations in the environment. Following the establishment of the mesocosm, five species of macroalgae that are common along the coast of Korea (namely Ulva pertusa, Codium fragile, Sargassum thunbergii, S. horneri, and Prionitis cornea) were exposed to three different CO₂ concentrations: ambient (×1) and elevated CO₂ (2× and 4× ambient), over two-week period, and their ecophysiological traits were measured. Results indicated that both photosynthesis and growth exhibited species-specific responses to the different CO₂ concentrations. Most notably, photosynthesis and growth increased in S. thunbergii when exposed to elevated CO₂ conditions but decreased in P. cornea. The preference for different inorganic carbon species (CO₂ and HCO₃⁻), which were estimated by gross photosynthesis in the presence and absence of the external carbonic anhydrase (eCA) inhibitor acetazolamide, were also found to vary among species and CO₂ treatments. Specifically, the two Sargassum species exhibited decreased eCA inhibition of photosynthesis with increased growth when exposed to high CO₂ conditions. In contrast, growth of U. pertusa and C. fragile were not notably affected by increased CO₂. Together, these results suggest that the five species of macroalgae may respond differently to changes in ocean acidity, with species-specific responses based on their differentiated photosynthetic acclimation. Understanding these physiological changes might allow us to better predict future changes in macroalgal communities in a more acidic ocean.

• ALGAE
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• 제23권4호
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• pp.311-316
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• 2008

The seasonal variation of marine algal biomass and community were studied with nondestructive sampling and quadrat method during May 2006 to April 2008 in the intertidal and subtidal zone at Yokjido. As result, total 120 species, 13 Chlorophyta, 34 Phaeophyta, 71 Rhodophyta and 2 Spermatophyta were identified in this study. The major species which contributed significantly to the total biomass were Ulva pertusa, Undaria pinnatifida, Ecklonia stolonifera, Sargassum horneri, S. serratifolium, Gelidium amansii and Prionitis cornea. The mean biomass was 235.5 g wet wt m$^{-2}$ in intertidal, 1,038.9 g wet wt m$^{-2}$ in 1 m, 1,013.1 g wet wt m$^{-2}$ in 5 m, and 19.7 g wet wt m$^{-2}$ in 10 m depths of mean sea level. Seasonal biomass change in intertidal zone, the peak season was in spring, while the lowest was in autumn. The vertical distribution of marine vegetation was characterized by Ulva pertusa - Gelidium divaricatum - Gloiopeltis furcata - Sargassum thunbergii - Hizikia fusiformis at intertidal zone, and Caulerpa okamurae - Gelidium amansii - Chondrus ocellatus - Ecklonia stolonifera - Sargassum horneri - Gracilaria textorii at subtidal zone. These result indicates that the marine algal species and biomass of 1 m and 5 m depths in subtidal zone are greater than intertidal zone, and Ecklonia stolonifera and Sargassum spp. are the dominant species of Yokjido.

• 한국수산과학회지
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• 제18권2호
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• pp.124-130
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• 1985

한국산 갈조류에서 추출한 알긴산의 우론산 조성 및 그 물성을 조사하고자 전보에 이어 괭생이모자반 및 외톨개모자반을 시료로 하여 채취 시기별에 따른 변화를 분석 검토하였다. 알긴산의 연평 균 함량은 외톨개모자반과 괭생이모자반인 각각 $25.2\%,\;26.5\%$이었으며, 연평균 M/G비는 외톨개모자반이 1.97, 괭생이모자반이 1.38이었다. 또한 M/G비의 연중 변화를 보면 외톨개모자반의 경우 최대치는 $12{\sim}l$월, 최소치는 $5{\sim}6$월, $10{\sim}11$월에 나타났다. 괭생이모자반의 경우는 1월에 최대치를 $3{\sim}4$월에 최소치를 나타내었다. 우론산 블럭 배렬비에 있어서는 alternating, M, G block이 외톨개모자반의 경우는 각각 $18.4\%,\;40.4\%,\;41.2\%$ 괭생이모자반의 경우는 각각 $9.8\%,\;33.3\%,\;56.9\%$로 나타났다. 외톨개모자반 알긴산의 점도는 11월 시료가 45.3cP로서 가장 높았으며, 괭생이모자반의 경우는 1월에 최고치 26.0cP를 나타내었다. 또한 점도의 온도의존성은 외톨개모자반의 경우는 11월 시료가 가장 컸으며 괭생이모자반의 경우는 6월 시료가 2월보다 다소 크게 나타났다. 금속이온교환량은 외톨개모자반의 경우 11월 시료가 가장 컸으며 그 교환량은 $Pb^{2+}\;4.4,\;Cu^{2+}\;1.8,\;Zn^{2+}\;2.5,\;Co^{2+}\;2.0\;meq/g. Na-Alg.$이었다. 괭생이모자반의 경우는 6월 시료가 2월 시료에 비하여 크게 나타났는데 그 교환량은 $Pb^{2+}\;4.5,\;Cu^{2+}\;2.2,\;Zn^{2+}\;2.4,\;Co^{2+}\;2.1\;meq/g. Na-Alg.$이었다. 대체적으로 보아 금속이온교환능은 우론산의 블럭비와 관련성이 있는 것으로 보인다.