• Asia-Pacific Journal of Business
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• v.12 no.2
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• pp.175-186
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• 2021

Purpose - This study intended to identify the effects of varied institutional systems and policies that regulate or define operations of enterprises upon the profitability of private enterprises through case analysis. Design/methodology/approach - The systems and differences in the institution and commodity distribution in Korea and Japan, which define the operation of the "Garak Wholesale Market" and "Ota Wholesale Market". We were examined in the presentthis study, and the statistical data pertinent to the trade volume and profitability of wholesale market corporations that substantially run both markets, were analyzed. Findings - The competition among the corporations that run the "Garak Wholesale Market" substantially is limited due to regulations over the mode of transaction and monopoly of trust thereby significantly higher level of profitability and safety are guaranteed. However, the institutional autonomy that enabled each distributor in the "Ota Wholesale Market" to compete with each other freely caused the distributors therein face difficulties in securing stable profits due to the relatively poor level of profitability resulted from the autonomy. Research implications or Originality - It seems the autonomy in commercial transaction in the "Garak Wholesale Market" in Korea needs to be secured to promote the competition of distributors therein through an amelioration of institutional systems and amendment of relevant laws that prescribe current management of various distributors/operators in the market.

• Journal of The Korean Society of Integrative Medicine
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• v.11 no.1
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• pp.99-111
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• 2023

Purpose : Although quantitative research on alcohol consumption among nursing students is important, qualitative research is needed to determine the subjective views of individual students, such as their feelings and thoughts, and ensure the implementation of a targeted alcohol intervention program. Q-methodology is a systematic approach that examines the subjective perspectives of individuals, including their views, beliefs, and attitudes, enabling understanding of the types and characteristics according to the individual's subjectivity structure. This study examined the subjective perceptions of drinking among freshmen in nursing college using Q methodology. Methods : Q-sorting was conducted, collecting 38 P samples and 40 statements. The data were analyzed using the PC QUANL program. The principal component factor analysis method was used for Q-factor analysis. Results : The results identified four types of drinking perceptions among freshmen in nursing college. Type 1 was "safety and health pursuit," type 2 was "friendship pursuit," type 3 was "'norm-oriented," and type 4 was "sound manners." When looking at the subjective perceptions of drinking among freshmen in nursing college, there was a common opinion that drinking should not be forced and that it is an individual choice. However, the difference in views (positive and negative) of drinking shows the need for customized educational programs and interventions suitable for each type. Conclusion : Nursing freshmen should be prepared to play an important role in health care as an educational role and model in preventing damage from drinking and maintaining health promotion throughout their life by habituating proper drinking behavior during college life. In addition, it is necessary to develop a plan to increase positive awareness of drinking among nursing students through various strategic programs that can participate in sobriety prevention programs within the university.

• Food Science of Animal Resources
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• v.43 no.3
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• pp.454-470
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• 2023

This study analyzed the microbiological (Lactobacillus spp., Staphylococcus spp., mold, yeast, aerobic bacteria) and physicochemical properties [pH, salinity, water activity, volatile basic nitrogen (VBN), and thiobarbituric acid reactive substances]. The starters were used by mixing Debaryomyces hansenii separated from Korean Doenjang (D) and fermented sausage (S). The starter was inoculated with dry-cured ham and aged for 6 weeks at 20℃ and 25℃, respectively. The aerobic bacteria, Lactobacillus spp., and Staphylococcus spp. of D, S, and DS treatment showed significantly higher values at 25℃ than at 20℃. Among them, S25 treatment showed a high tendency. At week 6, the mold of the S25 treatment was significantly higher than the S20 treatment, and the yeast was higher in 25℃ than 20℃ (p<0.05). The pH of all treatment groups increased with the aging period. Compared with that at 25℃, the pH was significantly higher at 20℃ (p<0.05). The water activity showed a significant decrease as the aging period increased, and the treatment of D25, S20, and DS20 showed a significantly higher value at week 6 (p<0.05). Compared with that at 20℃, the VBN content was higher at 25℃. At week 6, the VBN contents of the C20, S25, and DS25 groups were higher than those of the other treatment groups. Therefore, inoculation of D. hansenii separated from fermented sausage produced in Korean starter at 25℃ is expected to improve the safety of harmful microorganisms and physiochemical properties in dry-cured ham.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.700-718
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• 2003

Fructan, a polysaccharide existing in plants or produced by microorganisms, is a sugar polymer of fructose with $\beta$-2,6 linkages. In this study, we investigated some cosmeceutical properties of Fructan such as moisturizing effect, cell proliferation effect, anti-inflammation effect and cell cytotoxicity. Zymomonas mobilis, a microorganism producing Fructan, was cultured in a medium containing 10％ sucrose and 2％ yeast extract as main components for 24 hours at 37$^{\circ}C$ and pH 7. Fructan was obtained by precipitation from the cultured medium by adding alcohol (alcohol ratio of 1:3) after removing the enzyme by centrifuging. Fructan exhibited almost same moisturizing effect as hyaluronic acid and cell proliferation effect on human fibroblast and keratinocyte as well. Moreover, on cell proliferation test on bio-artificial skin constructed by 3-dimensional(3-D) culture after inducing primary skin inflammation with 0.5％ sodium lauryl sulfate (SLS), the 3-D artificial skin treated with 0.01 mg/ml, 0.05mg/ml of Fructan exhibited higher cell proliferation than the 3-D artificial skin treated with SLS only. On anti-inflammation test on 3-D artificial skin evaluated by measuring secreted quantity of interleukin-1$\alpha$ (IL-1$\alpha$) which is a pre-inflammatory mediator induced by SLS, the quantity of IL-1$\alpha$on the 3-D artificial skin treated with 0.01 mg/ml, 0.05mg/ml of Fructan was less than the one on the 3-D artificial skin treated with SLS only. As a result of these studies, Fructan has anti-inflammation effect against inflammatory reaction by a skin irritant as well as cell proliferation effect in bio-artificial skin. Fructan was also evaluated as a safe material without any toxicity in safety tests using fibroblasts and animals.

• Korean Journal of Transplantation
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• v.31 no.4
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• pp.157-169
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• 2017

Regulatory T cells (Treg) naturally rein in immune attacks, and they can inhibit rejection of transplanted organs and even reverse the progression of autoimmune diseases in mice. The initial safety trials of Treg against graft-versus-host disease (GVHD) provided evidence that the adoptive transfer of Treg is safe and capable of limiting disease progression. Supported by such evidence, numerous clinical trials have been actively investigating the efficacy of Treg targeting autoimmune diseases, type I diabetes, and organ transplant rejection, including kidney and liver. The limited quantity of Treg cells harvested from peripheral blood and subsequent in vitro culture have posed a great challenge to large-scale clinical application of Treg; nevertheless, the concept of CAR (chimeric antigen receptor)-Treg has emerged as a potential resolution to the problem. Recently, two CAR-T therapies, tisagenlecleucel and axicabtagene ciloleucel, were approved by the US FDA for the treatment of refractory or recurrent acute lymhoblastic leukemia. This approval could serve as a guideline for the production protocols for other genetically engineered T cells for clinical use as well. The phase I and II clinical trials of these agents has demonstrated that genetically engineered and antigen-targeting T cells are safe and efficacious in humans. In conclusion, both the promising results of Treg cell therapy from the clinical studies and the recent FDA approval of CAR-T therapies are paving the way for CAR-Treg therapy in clinical use.

• Journal of the Society of Disaster Information
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• v.19 no.2
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• pp.352-362
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• 2023

Purpose: For effective crisis communication in the event of a disaster, we present policy measures by exploring what activities are required of companies on a daily basis. Method: Employees working for L company were surveyed about the disaster response department, disaster message, and perception of the company during the company's response to COVID-19, as well as the level of response behavior of the employee. Results: First, caller characteristics did not have a significant effect on behavioral changes of members. Second, while the disaster response message within the company had no effect on the members' normative behavior, it had a positive effect on the discretionary behavior. Third, caller characteristics and disaster response messages had a positive effect on public relations, and public relations appeared to mediate caller characteristics and members' discretionary behavior. Conclusion: Studies on disaster response messages are required in the disaster control tower inside the company in normal times, and activities to build a member-friendly corporate culture for strengthening public relations are needed.

• Journal of The Geomorphological Association of Korea
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• v.26 no.4
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• pp.123-140
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• 2019

This study analyzed problems of interpretive signs along Eorimok-Yeongsil Trail on Mt. Hallasan National Park, focusing on factors of distracting visitor's attention from reading and understanding the signs. Eorimok-Yeongsil Trail has a total of 86 signs, and according to their purpose and content, they are classified into thirteen general signs, sixteen location signs, nine safety signs and forty-eight interpretive signs. Interpretive signs provide visitors with information and explanations about the nature and human resources of Mt. Hallasan as well as enhancing the opportunity for self-guided interpretation. The contents of interpretive signs of Eorimok-Yeongsil Trail are composed of landscapes, flora and fauna, topography, geology, history and culture, of which 70.8% of the total are related to plants and animals. Interpretive signs on the Eorimok-Yeongsil Trail do not attract visitors' attention because of the low readability, inappropriate locations and frequent errors in translation from Korean into other languages. The causes of low readability of interpretive signs include thirty-six physically damaged and faded signs, twenty-two improper font sizes and misuse of color schemes and five unclear description signs. The reasons for the incorrect locations include twenty-two long distances from the trails, twenty-one inadequate heights and fifteen inconsistencies in descriptions and locations. Problems with English use include eight grammar and spelling errors, twenty-one incorrect translations, and twenty-six English names missing.

• Journal of dental hygiene science
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• v.23 no.4
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• pp.369-377
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• 2023

Background: In this study, we investigated the potential use of keratin for oral tissue regeneration. Keratin is well-known for its effectiveness in skin regeneration by promoting keratinization and enhancing the elasticity and activity of fibroblasts. Because of its structural stability, high storability, biocompatibility, and safety in humans, existing research has predominantly focused on its role in skin wound healing. Herein, we propose using keratin proteins as biocompatible materials for dental applications. Methods: To assess the suitability of alpha-keratin protein as a substrate for cell culture, keratin was extracted from human hair via PEGylation. Viabilities of primary human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs) were assessed. Fluorescence immunostaining and migration assays were conducted using a fluorescence microscope and confocal laser scanning microscope. Wound healing and migration assays were performed using automated software to analyze the experimental readout and gap closure rate. Results: We confirmed the extraction of alpha-keratin and formation of the PEG-g-keratin complex. Treatment of HGFs with keratin protein at a concentration of 5 mg/ml promoted proliferation and maintained cell viability in the test group compared to the control group. HOKs treated with 5 mg/ml keratin exhibited a slight decrease in cell proliferation and activity after 48 hours compared to the untreated group, followed by an increase after 72 hours. Wound healing and migration assays revealed rapid closure of the area covered by HOKs over time following keratin treatment. Additionally, HOKs exhibited changes in cell morphology and increased the expression of the mesenchymal marker vimentin. Conclusion: Our study demonstrated the potential of hair keratin for soft tissue regeneration, with potential future applications in clinical settings for wound healing.

• The Journal of the Korea institute of electronic communication sciences
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• v.19 no.1
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• pp.197-208
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• 2024

In today's era, where internet technology and culture are advancing rapidly, the importance of systematic internet ethics education has been underscored to address the insufficient levels of internet ethics among college students. This research primarily focused on assessing the level of internet ethics among college students, who represent a significant proportion of internet users. The innovative Flip Learning teaching method and the introduction of the Wheeler Model, along with the application of frequency measurement theory, allowed us to explore ways to maximize the effectiveness of the education. It was confirmed that students who received internet ethics education demonstrated improved levels of ethics. This study concluded that significant progress has been made in systematizing and effectively promoting internet ethics education. It also proposed the need for systematic ethics education and provided future directions for improving internet ethics education.

• Journal of Microbiology and Biotechnology
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• v.34 no.5
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• pp.1017-1028
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• 2024

Lignocellulolytic enzymes play a crucial role in efficiently converting lignocellulose into valuable platform molecules in various industries. However, they are limited by their production yields, costs, and stability. Consequently, their production by producers adapted to local environments and the choice of low-cost raw materials can address these limitations. Due to the large amounts of olive stones (OS) generated in Morocco which are still undervalued, Penicillium crustosum, Fusarium nygamai, Trichoderma capillare, and Aspergillus calidoustus, are cultivated under different fermentation techniques using this by-product as a local lignocellulosic substrate. Based on a multilevel factorial design, their potential to produce lignocellulolytic enzymes during 15 days of dark incubation was evaluated. The results revealed that P. crustosum expressed a maximum total cellulase activity of 10.9 IU/ml under sequential fermentation (SF) and 3.6 IU/ml of β-glucosidase activity under submerged fermentation (SmF). F. nygamai recorded the best laccase activity of 9 IU/ml under solid-state fermentation (SSF). Unlike T. capillare, SF was the inducive culture for the former activity with 7.6 IU/ml. A. calidoustus produced, respectively, 1,009 ㎍/ml of proteins and 11.5 IU/ml of endoglucanase activity as the best results achieved. Optimum cellulase production took place after the 5th day under SF, while ligninases occurred between the 9th and the 11th days under SSF. This study reports for the first time the lignocellulolytic activities of F. nygamai and A. calidoustus. Furthermore, it underlines the potential of the four fungi as biomass decomposers for environmentally-friendly applications, emphasizing the efficiency of OS as an inducing substrate for enzyme production.